Experts and Doctors on cell line in Scotland, United Kingdom

Summary

Locale: Scotland, United Kingdom
Topic: cell line

Top Publications

  1. Deak M, Clifton A, Lucocq L, Alessi D. Mitogen- and stress-activated protein kinase-1 (MSK1) is directly activated by MAPK and SAPK2/p38, and may mediate activation of CREB. EMBO J. 1998;17:4426-41 pubmed
    ..These findings, together with other observations, suggest that MSK1 may mediate the growth-factor and stress-induced activation of CREB. ..
  2. Bain J, Plater L, Elliott M, Shpiro N, Hastie C, McLauchlan H, et al. The selectivity of protein kinase inhibitors: a further update. Biochem J. 2007;408:297-315 pubmed
    ..Despite being used widely, many of the compounds that we analysed were too non-specific for useful conclusions to be made, other than to exclude the involvement of particular protein kinases in cellular processes. ..
  3. Fant X, Samejima K, Carvalho A, Ogawa H, Xu Z, Yue Z, et al. Use of DT40 conditional-knockout cell lines to study chromosomal passenger protein function. Biochem Soc Trans. 2010;38:1655-9 pubmed publisher
    ..These lines enabled us to reassess current knowledge of survivin function and to show that INCENP acts as a rheostat for Aurora B activity. ..
  4. Szemiel A, Failloux A, Elliott R. Role of Bunyamwera Orthobunyavirus NSs protein in infection of mosquito cells. PLoS Negl Trop Dis. 2012;6:e1823 pubmed publisher
    ..Bunyamwera virus NSs is required for efficient replication in certain mosquito cell lines and in Ae. aegypti mosquitoes. ..
  5. Vitari A, Deak M, Collins B, Morrice N, Prescott A, Phelan A, et al. WNK1, the kinase mutated in an inherited high-blood-pressure syndrome, is a novel PKB (protein kinase B)/Akt substrate. Biochem J. 2004;378:257-68 pubmed
    ..These results provide the first connection between the PI 3-kinase/PKB pathway and WNK1, suggesting a mechanism by which this pathway may influence blood pressure. ..
  6. Cazalla D, Sanford J, Caceres J. A rapid and efficient protocol to purify biologically active recombinant proteins from mammalian cells. Protein Expr Purif. 2005;42:54-8 pubmed
    ..In summary, we present a protocol based on transient transfection of mammalian cells that results in easy purification of significant amounts of biologically active proteins. ..
  7. Cadalbert L, Sloss C, Cameron P, Plevin R. Conditional expression of MAP kinase phosphatase-2 protects against genotoxic stress-induced apoptosis by binding and selective dephosphorylation of nuclear activated c-jun N-terminal kinase. Cell Signal. 2005;17:1254-64 pubmed
    ..These studies demonstrate the in vivo specificity of MKP-2 for JNK and not ERK and show that nuclear-targeted JNK is involved in genotoxic stress-induced apoptosis. ..
  8. McCoy C, Macdonald A, Morrice N, Campbell D, Deak M, Toth R, et al. Identification of novel phosphorylation sites in MSK1 by precursor ion scanning MS. Biochem J. 2007;402:491-501 pubmed
  9. Festuccia N, Chambers I. Quantification of pluripotency transcription factor levels in embryonic stem cells by flow cytometry. Curr Protoc Stem Cell Biol. 2011;Chapter 1:Unit 1B.9 pubmed publisher
    ..We illustrate the application of this technique to the detection of Oct4 and Nanog proteins and the coupling of this approach with fluorescent reporters of gene activity. ..

More Information

Publications290 found, 100 shown here

  1. Miele G, Manson J, Clinton M. A novel erythroid-specific marker of transmissible spongiform encephalopathies. Nat Med. 2001;7:361-4 pubmed
    ..Our findings indicate a previously unrecognized role for involvement of the erythroid lineage in the etiology of TSE pathogenesis and should provide a new focus for research into diagnostic and therapeutic strategies. ..
  2. Leslie N, Bennett D, Gray A, Pass I, Hoang Xuan K, Downes C. Targeting mutants of PTEN reveal distinct subsets of tumour suppressor functions. Biochem J. 2001;357:427-35 pubmed
    ..Our data show that deregulation of PKB is not a universal feature of tumours carrying PTEN mutations and implicate other processes that may be deregulated in these tumours. ..
  3. Mulero V, Searle S, Blackwell J, Brock J. Solute carrier 11a1 (Slc11a1; formerly Nramp1) regulates metabolism and release of iron acquired by phagocytic, but not transferrin-receptor-mediated, iron uptake. Biochem J. 2002;363:89-94 pubmed
  4. Weber M, Blair E, Simpson C, O Hara M, Blackburn P, Rot A, et al. The chemokine receptor D6 constitutively traffics to and from the cell surface to internalize and degrade chemokines. Mol Biol Cell. 2004;15:2492-508 pubmed
    ..By this mechanism, D6 can target chemokines for degradation without the necessity for cell signaling, and without desensitizing the cell to subsequent chemokine exposure. ..
  5. Zeke T, Morrice N, Vázquez Martin C, Cohen P. Human protein phosphatase 5 dissociates from heat-shock proteins and is proteolytically activated in response to arachidonic acid and the microtubule-depolymerizing drug nocodazole. Biochem J. 2005;385:45-56 pubmed
  6. Lowen A, Noonan C, McLees A, Elliott R. Efficient bunyavirus rescue from cloned cDNA. Virology. 2004;330:493-500 pubmed
    ..We suggest that this procedure should be applicable to viruses in other genera of the family Bunyaviridae and perhaps also to arenaviruses. ..
  7. Li C, Potuschak T, COLON CARMONA A, Gutierrez R, Doerner P. Arabidopsis TCP20 links regulation of growth and cell division control pathways. Proc Natl Acad Sci U S A. 2005;102:12978-83 pubmed
  8. Everett R. Interactions between DNA viruses, ND10 and the DNA damage response. Cell Microbiol. 2006;8:365-74 pubmed
    ..Similarly, PML and DNA repair proteins are recruited to sites of DNA damage. The mechanisms by which these events might occur, and the implications for ND10 function in DNA virus infection and chromatin metabolism, are discussed...
  9. Precious B, Carlos T, Goodbourn S, Randall R. Catalytic turnover of STAT1 allows PIV5 to dismantle the interferon-induced anti-viral state of cells. Virology. 2007;368:114-21 pubmed
    ..The importance of this mechanism in terms of the ability of the virus to dismantle the IFN-induced anti-viral state of cells is discussed. ..
  10. Boutell C, Everett R, Hilliard J, Schaffer P, Orr A, Davido D. Herpes simplex virus type 1 ICP0 phosphorylation mutants impair the E3 ubiquitin ligase activity of ICP0 in a cell type-dependent manner. J Virol. 2008;82:10647-56 pubmed publisher
    ..This study suggests that specific cellular environments and context of expression (transfection versus infection) differentially regulate several activities of ICP0 related to its E3 ubiquitin ligase activity via phosphorylation. ..
  11. Calcraft P, Ruas M, Pan Z, Cheng X, Arredouani A, Hao X, et al. NAADP mobilizes calcium from acidic organelles through two-pore channels. Nature. 2009;459:596-600 pubmed publisher
    ..TPCs therefore provide new insights into the regulation and organization of Ca(2+) signals in animal cells, and will advance our understanding of the physiological role of NAADP...
  12. Munoz I, Hain K, Déclais A, Gardiner M, Toh G, Sanchez Pulido L, et al. Coordination of structure-specific nucleases by human SLX4/BTBD12 is required for DNA repair. Mol Cell. 2009;35:116-27 pubmed publisher
    ..Depletion of SLX4 causes a decrease in DSB-induced homologous recombination. These data show that SLX4 is a regulator of structure-specific nucleases and that SLX4 and SLX1 are important regulators of genome stability in human cells. ..
  13. Xu T, Baillie G, Bhari N, Houslay T, Pitt A, Adams D, et al. Mutations of beta-arrestin 2 that limit self-association also interfere with interactions with the beta2-adrenoceptor and the ERK1/2 MAPKs: implications for beta2-adrenoceptor signalling via the ERK1/2 MAPKs. Biochem J. 2008;413:51-60 pubmed publisher
    ..Regulation of beta-arrestin 2 self-association may therefore control beta-arrestin 2-mediated beta2-adrenoceptor-ERK1/2 MAPK signalling. ..
  14. Selby C, Drost E, Brown D, Howie S, MacNee W. Inhibition of neutrophil adherence and movement by acute cigarette smoke exposure. Exp Lung Res. 1992;18:813-27 pubmed
    ..These data suggest that acute cigarette smoke may impair the crucial neutrophil functions of adherence and movement. However, the chronic effects of cigarette smoke exposure may clearly differ. ..
  15. Dani C, Chambers I, Johnstone S, Robertson M, Ebrahimi B, Saito M, et al. Paracrine induction of stem cell renewal by LIF-deficient cells: a new ES cell regulatory pathway. Dev Biol. 1998;203:149-62 pubmed
    ..Operation of this pathway in vivo could play an important role in the regulation of pluripotency in the epiblast and account for the viability of lifr -/- and gp130 -/- embryos. ..
  16. Sapkota G, Kieloch A, Lizcano J, Lain S, Arthur J, Williams M, et al. Phosphorylation of the protein kinase mutated in Peutz-Jeghers cancer syndrome, LKB1/STK11, at Ser431 by p90(RSK) and cAMP-dependent protein kinase, but not its farnesylation at Cys(433), is essential for LKB1 to suppress cell vrowth. J Biol Chem. 2001;276:19469-82 pubmed
    ..In contrast, a mutant of LKB1 that cannot be prenylated was still able to suppress the growth of cells. ..
  17. Boudeau J, Deak M, Lawlor M, Morrice N, Alessi D. Heat-shock protein 90 and Cdc37 interact with LKB1 and regulate its stability. Biochem J. 2003;370:849-57 pubmed
    ..Hsp90 inhibitors are being considered as potential anti-cancer agents. However, our observations indicate that prolonged usage of these drugs could possibly lead to tumour development by decreasing cellular levels of LKB1. ..
  18. Rutherford C, Ord Shrimpton F, Sands W, Pediani J, Benovic J, McGrath J, et al. Phosphorylation-independent internalisation and desensitisation of the human sphingosine-1-phosphate receptor S1P3. Cell Signal. 2005;17:997-1009 pubmed
  19. Harrison B, Kraus M, Burch L, Stevens C, Craig A, Gordon Weeks P, et al. DAPK-1 binding to a linear peptide motif in MAP1B stimulates autophagy and membrane blebbing. J Biol Chem. 2008;283:9999-10014 pubmed publisher
    ..These data highlight the utility of peptide aptamers to identify novel binding interfaces and highlight a role for MAP1B in DAPK-1-dependent signaling in autophagy and membrane blebbing. ..
  20. Yue Z, Carvalho A, Xu Z, Yuan X, Cardinale S, Ribeiro S, et al. Deconstructing Survivin: comprehensive genetic analysis of Survivin function by conditional knockout in a vertebrate cell line. J Cell Biol. 2008;183:279-96 pubmed publisher
  21. Paytubi S, Wang X, Lam Y, Izquierdo L, Hunter M, Jan E, et al. ABC50 promotes translation initiation in mammalian cells. J Biol Chem. 2009;284:24061-73 pubmed publisher
    ..Taken together, the present data show that ABC50 plays a key role in translation initiation and has functions that are distinct from those of other non-membrane ABC proteins...
  22. Wadd S, Bryant H, Filhol O, Scott J, Hsieh T, Everett R, et al. The multifunctional herpes simplex virus IE63 protein interacts with heterogeneous ribonucleoprotein K and with casein kinase 2. J Biol Chem. 1999;274:28991-8 pubmed
    ..These data provide important new insights into how this key viral regulatory protein exerts its functions. ..
  23. Everett R, Sourvinos G, Leiper C, Clements J, Orr A. Formation of nuclear foci of the herpes simplex virus type 1 regulatory protein ICP4 at early times of infection: localization, dynamics, recruitment of ICP27, and evidence for the de novo induction of ND10-like complexes. J Virol. 2004;78:1903-17 pubmed
    ..This response is modulated by functional ICP0, and cells that are productively or nonproductively infected in the absence of functional ICP0 can be distinguished by the relative locations of ICP4 foci and ND10 proteins. ..
  24. Schuttelkopf A, Hamilton G, Watts C, van Aalten D. Structural basis of reduction-dependent activation of human cystatin F. J Biol Chem. 2006;281:16570-5 pubmed
  25. Bennett L, Larkin C, Gillespie D, Clarke P. Claspin is phosphorylated in the Chk1-binding domain by a kinase distinct from Chk1. Biochem Biophys Res Commun. 2008;369:973-6 pubmed publisher
    ..These results demonstrate that phosphorylation of Claspin within the Chk1-binding domain is catalysed by an ATR-dependent kinase distinct from Chk1. ..
  26. Shi X, Elliott R. Generation and analysis of recombinant Bunyamwera orthobunyaviruses expressing V5 epitope-tagged L proteins. J Gen Virol. 2009;90:297-306 pubmed publisher
    ..The recombinant viruses expressing tagged L protein will be highly valuable reagents for the detailed dissection of the role of the BUNV L protein in virus replication. ..
  27. Clark K, Plater L, Peggie M, Cohen P. Use of the pharmacological inhibitor BX795 to study the regulation and physiological roles of TBK1 and IkappaB kinase epsilon: a distinct upstream kinase mediates Ser-172 phosphorylation and activation. J Biol Chem. 2009;284:14136-46 pubmed publisher
  28. Jovanović S, Du Q, Sukhodub A, Jovanović A. M-LDH physically associated with sarcolemmal K ATP channels mediates cytoprotection in heart embryonic H9C2 cells. Int J Biochem Cell Biol. 2009;41:2295-301 pubmed publisher
    ..2/SUR2A regulates the activity of sarcolemmal K(ATP) channels as well as an intracellular ATP production during metabolic stress, both of which are important for cell survival. ..
  29. Bennett K, Langmead C, Wise A, Milligan G. Growth hormone secretagogues and growth hormone releasing peptides act as orthosteric super-agonists but not allosteric regulators for activation of the G protein Galpha(o1) by the Ghrelin receptor. Mol Pharmacol. 2009;76:802-11 pubmed publisher
  30. Cohen P. Guidelines for the effective use of chemical inhibitors of protein function to understand their roles in cell regulation. Biochem J. 2009;425:53-4 pubmed publisher
    ..In the present paper, using protein kinase inhibitors as the example, a set of criteria are described that should be met before any investigation using such compounds should be accepted for publication. ..
  31. Ohta S, Bukowski Wills J, Wood L, de Lima Alves F, Chen Z, Rappsilber J, et al. Proteomics of isolated mitotic chromosomes identifies the kinetochore protein Ska3/Rama1. Cold Spring Harb Symp Quant Biol. 2010;75:433-8 pubmed publisher
    ..The approach presented here offers a powerful way to define the functional proteome of complex organelles and structures whose composition is not simple or fixed. ..
  32. Durrani Z, Weir W, Pillai S, Kinnaird J, Shiels B. Modulation of activation-associated host cell gene expression by the apicomplexan parasite Theileria annulata. Cell Microbiol. 2012;14:1434-54 pubmed publisher
  33. Festuccia N, Osorno R, Halbritter F, Karwacki Neisius V, Navarro P, Colby D, et al. Esrrb is a direct Nanog target gene that can substitute for Nanog function in pluripotent cells. Cell Stem Cell. 2012;11:477-90 pubmed publisher
    ..Finally, Esrrb deletion abolishes the defining ability of Nanog to confer LIF-independent ESC self-renewal. These findings are consistent with the functional placement of Esrrb downstream of Nanog...
  34. Smyth A, Yang L, Martin K, Hamilton C, Lu W, Cousin M, et al. Munc18-1 protein molecules move between membrane molecular depots distinct from vesicle docking sites. J Biol Chem. 2013;288:5102-13 pubmed publisher
    ..Our findings demonstrate that Munc18-1 molecules move between membrane depots distinct from vesicle morphological docking sites. ..
  35. Casamayor A, Morrice N, Alessi D. Phosphorylation of Ser-241 is essential for the activity of 3-phosphoinositide-dependent protein kinase-1: identification of five sites of phosphorylation in vivo. Biochem J. 1999;342 ( Pt 2):287-92 pubmed
    ..PDK1 expressed in bacteria was active and phosphorylated at Ser-241, suggesting that PDK1 can phosphorylate itself at this site, leading to its own activation. ..
  36. Parkinson J, Everett R. Alphaherpesvirus proteins related to herpes simplex virus type 1 ICP0 affect cellular structures and proteins. J Virol. 2000;74:10006-17 pubmed
  37. McPhillips M, Veerapraditsin T, Cumming S, Karali D, Milligan S, Boner W, et al. SF2/ASF binds the human papillomavirus type 16 late RNA control element and is regulated during differentiation of virus-infected epithelial cells. J Virol. 2004;78:10598-605 pubmed
  38. Kuma Y, Sabio G, Bain J, Shpiro N, Marquez R, Cuenda A. BIRB796 inhibits all p38 MAPK isoforms in vitro and in vivo. J Biol Chem. 2005;280:19472-9 pubmed
  39. Lowen A, Boyd A, Fazakerley J, Elliott R. Attenuation of bunyavirus replication by rearrangement of viral coding and noncoding sequences. J Virol. 2005;79:6940-6 pubmed
    ..Since BUN MLM virus is attenuated due to an essentially irreversible mutation, the rearrangement of UTRs is a feasible strategy for vaccine design for the more pathogenic members of the Bunyaviridae. ..
  40. Shi X, Brauburger K, Elliott R. Role of N-linked glycans on bunyamwera virus glycoproteins in intracellular trafficking, protein folding, and virus infectivity. J Virol. 2005;79:13725-34 pubmed
    ..The viability of these recombinant viruses and analysis of growth kinetics indicates that the glycans on Gc are not essential for BUN replication, but they do contribute to the efficiency of virus infection. ..
  41. Windheim M, Lang C, Peggie M, Plater L, Cohen P. Molecular mechanisms involved in the regulation of cytokine production by muramyl dipeptide. Biochem J. 2007;404:179-90 pubmed
  42. Holman H, MacLean A. Neurovirulent factor ICP34.5 uniquely expressed in the herpes simplex virus type 1 Delta gamma 1 34.5 mutant 1716. J Neurovirol. 2008;14:28-40 pubmed publisher
    ..5 gene and restore most (but not all) wild-type function. These findings are discussed with respect to the use of the gamma(1)34.5 deleted mutant, 1716, in oncolytic viral vector therapies and future studies for ICP34.5. ..
  43. García Martínez J, Alessi D. mTOR complex 2 (mTORC2) controls hydrophobic motif phosphorylation and activation of serum- and glucocorticoid-induced protein kinase 1 (SGK1). Biochem J. 2008;416:375-85 pubmed publisher
    ..The results of the present study indicate that NDRG1 phosphorylation represents an excellent biomarker for mTORC2 activity. ..
  44. Narayan V, Eckert M, Zylicz A, Zylicz M, Ball K. Cooperative regulation of the interferon regulatory factor-1 tumor suppressor protein by core components of the molecular chaperone machinery. J Biol Chem. 2009;284:25889-99 pubmed publisher
    ..This study begins to elucidate the role of the Mf1 domain of IRF-1 in orchestrating the recruitment of regulatory factors that can impact on both its turnover and transcriptional activity. ..
  45. Robertson J, Lang S, Lambert P, Martin P. Peptidoglycan derived from Staphylococcus epidermidis induces Connexin43 hemichannel activity with consequences on the innate immune response in endothelial cells. Biochem J. 2010;432:133-43 pubmed publisher
    ..All ATP responses were blocked with Cx-specific channel blockers. We conclude that acute Cx43 hemichannel signalling plays a role in the initiation of early innate immune responses in the endothelium. ..
  46. Ge X, Blow J. Chk1 inhibits replication factory activation but allows dormant origin firing in existing factories. J Cell Biol. 2010;191:1285-97 pubmed publisher
    ..This minimizes the deleterious consequences of fork stalling and prevents similar problems from arising in unreplicated regions of the genome. ..
  47. de los Heros P, Alessi D, Gourlay R, Campbell D, Deak M, MacArtney T, et al. The WNK-regulated SPAK/OSR1 kinases directly phosphorylate and inhibit the K+-Cl- co-transporters. Biochem J. 2014;458:559-73 pubmed publisher
  48. McLean C, Bucheton A, Finnegan D. The 5' untranslated region of the I factor, a long interspersed nuclear element-like retrotransposon of Drosophila melanogaster, contains an internal promoter and sequences that regulate expression. Mol Cell Biol. 1993;13:1042-50 pubmed
    ..In transformed flies, high levels of expression from nucleotides +1 to +186 are confined to the ovaries of reactive females, suggesting that the promoter is involved in the tissue and cytotype specificity of transposition. ..
  49. Smith K, Scotland G, Beattie J, Trayer I, Houslay M. Determination of the structure of the N-terminal splice region of the cyclic AMP-specific phosphodiesterase RD1 (RNPDE4A1) by 1H NMR and identification of the membrane association domain using chimeric constructs. J Biol Chem. 1996;271:16703-11 pubmed
  50. Pleass R, Andrews P, Kerr M, Woof J. Alternative splicing of the human IgA Fc receptor CD89 in neutrophils and eosinophils. Biochem J. 1996;318 ( Pt 3):771-7 pubmed
    ..Alternative splicing might therefore serve as a means of diversifying Fc alpha R structure and function. ..
  51. Brown C, Ilhan T, Kirvar E, Thomas M, Wilkie G, Leemans I, et al. Theileria lestoquardi and T. annulata in cattle, sheep, and goats. In vitro and in vivo studies. Ann N Y Acad Sci. 1998;849:44-51 pubmed
    ..The importance of these findings in discriminating parasites in epidemiological studies and the control of these diseases with cell culture vaccines is emphasized...
  52. Hendrich B, Bird A. Identification and characterization of a family of mammalian methyl-CpG binding proteins. Mol Cell Biol. 1998;18:6538-47 pubmed
    ..The data demonstrate that MBD2 and MBD4 bind specifically to methyl-CpG in vitro and in vivo and are therefore likely to be mediators of the biological consequences of the methylation signal. ..
  53. Ainsztein A, Kandels Lewis S, Mackay A, Earnshaw W. INCENP centromere and spindle targeting: identification of essential conserved motifs and involvement of heterochromatin protein HP1. J Cell Biol. 1998;143:1763-74 pubmed
    ..Surprisingly, this interaction does not appear to be involved in targeting INCENP to the centromeric heterochromatin, but may instead have a role in its transfer from the chromosomes to the anaphase spindle. ..
  54. Jedlitschky G, Cassidy A, Sales M, Pratt N, Burchell B. Cloning and characterization of a novel human olfactory UDP-glucuronosyltransferase. Biochem J. 1999;340 ( Pt 3):837-43 pubmed
    ..Furthermore, some steroids, especially androgens, some drugs and carcinogens were conjugated. The results support a role of the enzyme in olfactory perception and in protection of the neural system against airborne hazardous chemicals. ..
  55. Kimber W, Deak M, Prescott A, Alessi D. Interaction of the protein tyrosine phosphatase PTPL1 with the PtdIns(3,4)P2-binding adaptor protein TAPP1. Biochem J. 2003;376:525-35 pubmed
    ..Consistent with this notion we observed RNA-interference-mediated knock-down of TAPP1 in HEK-293 cells, enhanced activation and phosphorylation of PKB following IGF1 stimulation. ..
  56. Canning M, Boutell C, Parkinson J, Everett R. A RING finger ubiquitin ligase is protected from autocatalyzed ubiquitination and degradation by binding to ubiquitin-specific protease USP7. J Biol Chem. 2004;279:38160-8 pubmed
    ..This principle may extend to a number of cellular RING finger E3 ubiquitin ligase proteins that have analogous interactions with ubiquitin-specific cleavage enzymes. ..
  57. McNeill H, Knebel A, Arthur J, Cuenda A, Cohen P. A novel UBA and UBX domain protein that binds polyubiquitin and VCP and is a substrate for SAPKs. Biochem J. 2004;384:391-400 pubmed
    ..We suggest that SAKS1 may be an adaptor that directs VCP to polyubiquitinated proteins, and PNGase to misfolded glycoproteins, facilitating their destruction by the proteasome. ..
  58. Clarke C, Bennett L, Clarke P. Cleavage of claspin by caspase-7 during apoptosis inhibits the Chk1 pathway. J Biol Chem. 2005;280:35337-45 pubmed
    ..These results indicate that cleavage of Claspin by caspase-7 inactivates the Chk1 signaling pathway. This mechanism may regulate the balance between cell cycle arrest and induction of apoptosis during the response to genotoxic stress. ..
  59. Lane J, Powney B, Wise A, Rees S, Milligan G. Protean agonism at the dopamine D2 receptor: (S)-3-(3-hydroxyphenyl)-N-propylpiperidine is an agonist for activation of Go1 but an antagonist/inverse agonist for Gi1,Gi2, and Gi3. Mol Pharmacol. 2007;71:1349-59 pubmed
    ..These results demonstrate (S)-(-)-3-(3-hydroxyphenyl)-N-propylpiperidine to be a protean agonist at the D(2) receptor and may explain in vivo actions of this ligand. ..
  60. Pearce L, Huang X, Boudeau J, Pawłowski R, Wullschleger S, Deak M, et al. Identification of Protor as a novel Rictor-binding component of mTOR complex-2. Biochem J. 2007;405:513-22 pubmed
    ..Protor-1 is a novel Rictor-binding subunit of mTORC2, but further work is required to establish its role. ..
  61. Long J, Pyne N, Pyne S. Lipid phosphate phosphatases form homo- and hetero-oligomers: catalytic competency, subcellular distribution and function. Biochem J. 2008;411:371-7 pubmed publisher
  62. Canals M, Lopez Gimenez J, Milligan G. Cell surface delivery and structural re-organization by pharmacological chaperones of an oligomerization-defective alpha(1b)-adrenoceptor mutant demonstrates membrane targeting of GPCR oligomers. Biochem J. 2009;417:161-72 pubmed publisher
  63. Everett R, Parsy M, Orr A. Analysis of the functions of herpes simplex virus type 1 regulatory protein ICP0 that are critical for lytic infection and derepression of quiescent viral genomes. J Virol. 2009;83:4963-77 pubmed publisher
  64. Mazel Sanchez B, Elliott R. Attenuation of bunyamwera orthobunyavirus replication by targeted mutagenesis of genomic untranslated regions and creation of viable viruses with minimal genome segments. J Virol. 2012;86:13672-8 pubmed publisher
    ..Although the role of this domain remains unknown, we suggest that those changes might be involved in the evolution of the polymerase to recognize the deleted UTRs more efficiently. ..
  65. Elliott R, Blakqori G, van Knippenberg I, Koudriakova E, Li P, McLees A, et al. Establishment of a reverse genetics system for Schmallenberg virus, a newly emerged orthobunyavirus in Europe. J Gen Virol. 2013;94:851-9 pubmed publisher
    ..The development of a robust reverse genetics system for SBV will facilitate investigation of its pathogenic mechanisms as well as the creation of attenuated strains that could be candidate vaccines. ..
  66. Simpson C, Fothergill Gilmore L. Isolation and sequence of a cDNA encoding human platelet phosphofructokinase. Biochem Biophys Res Commun. 1991;180:197-203 pubmed
    ..The platelet gene has been assigned to chromosome 10p15.2-p15.3 by using the cDNA clone as a biotinylated probe against human chromosome spreads (Morrison et al. 1991, submitted to Human Genetics). ..
  67. Wooster R, Sutherland L, Ebner T, Clarke D, Da Cruz e Silva O, Burchell B. Cloning and stable expression of a new member of the human liver phenol/bilirubin: UDP-glucuronosyltransferase cDNA family. Biochem J. 1991;278 ( Pt 2):465-9 pubmed
    ..These results suggest that the two isoenzymes may be derived from the same gene by differential splicing of the gene product. ..
  68. Barber M, Travers M. Elucidation of a promoter activity that directs the expression of acetyl-CoA carboxylase alpha with an alternative N-terminus in a tissue-restricted fashion. Biochem J. 1998;333 ( Pt 1):17-25 pubmed
    ..Promoter activity is also evident in primary sheep mammary epithelial cells. These results demonstrate the presence of a third promoter, PIII, in the ACC-alpha gene that results in the tissue-restricted expression of an ACC isoenzyme. ..
  69. Tate P, Lee M, Tweedie S, Skarnes W, Bickmore W. Capturing novel mouse genes encoding chromosomal and other nuclear proteins. J Cell Sci. 1998;111 ( Pt 17):2575-85 pubmed
    ..Motifs in two of the isolated genes suggest new links between cellular regulatory mechanisms (ubiquitination and phosphorylation) and mRNA splicing and chromosome structure/function. ..
  70. Xirodimas D, Saville M, Edling C, Lane D, Lain S. Different effects of p14ARF on the levels of ubiquitinated p53 and Mdm2 in vivo. Oncogene. 2001;20:4972-83 pubmed
    ..These studies reveal important differences in the regulation of the stability of p53 and of Mdm2. ..
  71. Eskelinen E, Prescott A, Cooper J, Brachmann S, Wang L, Tang X, et al. Inhibition of autophagy in mitotic animal cells. Traffic. 2002;3:878-93 pubmed
    ..Our results show that autophagy is under strict mitotic control and indicate a novel role for phosphoinositide 3-kinases or other wortmannin/LY294002-sensitive kinases in mitotic membrane traffic regulation. ..
  72. Barber M, Vallance A, Kennedy H, Travers M. Induction of transcripts derived from promoter III of the acetyl-CoA carboxylase-alpha gene in mammary gland is associated with recruitment of SREBP-1 to a region of the proximal promoter defined by a DNase I hypersensitive site. Biochem J. 2003;375:489-501 pubmed
    ..This indicates that SREBP-1 is a major developmental regulator of the programme of lipid synthesis de novo in the lactating mammary gland. ..
  73. Jowsey P, Doherty A, Rouse J. Human PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. J Biol Chem. 2004;279:55562-9 pubmed
    ..Lowering hPTIP levels also increased cellular sensitivity to IR, suggesting that this protein plays a critical role in maintaining genome stability. ..
  74. Richard K, Ramminger S, Forsyth L, Burchell A, Wilson S. Thyroid hormone potentiates glucocorticoid-evoked airway Na+ transport without affecting alpha-ENaC transcription. FEBS Lett. 2004;576:339-42 pubmed
    ..The facilitation of Na+ transport may thus reflect control over events downstream to transcription. ..
  75. Padmanabhan U, Dollins D, Fridy P, York J, Downes C. Characterization of a selective inhibitor of inositol hexakisphosphate kinases: use in defining biological roles and metabolic relationships of inositol pyrophosphates. J Biol Chem. 2009;284:10571-82 pubmed publisher
    ..TNP thus provides a means of selectively and rapidly modulating cellular InsP(7) levels, providing a new and versatile tool to study the biological function and metabolic relationships of inositol pyrophosphates...
  76. Garcia Mata C, Wang J, Gajdanowicz P, Gonzalez W, Hills A, Donald N, et al. A minimal cysteine motif required to activate the SKOR K+ channel of Arabidopsis by the reactive oxygen species H2O2. J Biol Chem. 2010;285:29286-94 pubmed publisher
    ..These, and additional data identify Cys(168) as a critical target for H(2)O(2), and implicate ROS-mediated control of the K(+) channel in regulating mineral nutrient partitioning within the plant. ..
  77. Pou C, Mannoury La Cour C, Stoddart L, Millan M, Milligan G. Functional homomers and heteromers of dopamine D2L and D3 receptors co-exist at the cell surface. J Biol Chem. 2012;287:8864-78 pubmed publisher
    ..These data are of potential importance both to disorders in which D(2long) and D(3) receptors are implicated, like schizophrenia and Parkinson disease, and also to drugs exerting their actions via these sites. ..
  78. Bridgen A, Weber F, Fazakerley J, Elliott R. Bunyamwera bunyavirus nonstructural protein NSs is a nonessential gene product that contributes to viral pathogenesis. Proc Natl Acad Sci U S A. 2001;98:664-9 pubmed
    ..These results suggest that, although not essential for growth in tissue culture or in mice, the bunyavirus NSs protein has several functions in the virus life cycle and contributes to viral pathogenesis...
  79. Bonnesen C, Eggleston I, Hayes J. Dietary indoles and isothiocyanates that are generated from cruciferous vegetables can both stimulate apoptosis and confer protection against DNA damage in human colon cell lines. Cancer Res. 2001;61:6120-30 pubmed
    ..Similar results were obtained with H(2)O(2) as the genotoxic agent. Together, these phytochemicals may prevent colon tumorigenesis by both stimulating apoptosis and enhancing intracellular defenses against genotoxic agents. ..
  80. Tian L, Coghill L, MacDonald S, Armstrong D, Shipston M. Leucine zipper domain targets cAMP-dependent protein kinase to mammalian BK channels. J Biol Chem. 2003;278:8669-77 pubmed
    ..Thus LZ1 is required for the association and regulation of mammalian BK channels by PKA, and other putative leucine zippers in the BK channel protein may provide anchoring for other regulatory enzyme complexes. ..
  81. Moore R, Redhead N, Selfridge J, Hope J, Manson J, Melton D. Double replacement gene targeting for the production of a series of mouse strains with different prion protein gene alterations. Biotechnology (N Y). 1995;13:999-1004 pubmed
    ..We anticipate that this procedure will have applications to the study of human inherited diseases and the development of therapies. ..
  82. Chatziandreou N, Young D, Andrejeva J, Goodbourn S, Randall R. Differences in interferon sensitivity and biological properties of two related isolates of simian virus 5: a model for virus persistence. Virology. 2002;293:234-42 pubmed
  83. Bridgen A, Dalrymple D, Elliott R. Dugbe nairovirus S segment: correction of published sequence and comparison of five isolates. Virology. 2002;294:364-71 pubmed
    ..Phylogenetic trees were constructed for these nairovirus S segment sequences. ..
  84. Moore W, Zhang C, Clarke P. Targeting of RCC1 to chromosomes is required for proper mitotic spindle assembly in human cells. Curr Biol. 2002;12:1442-7 pubmed
    ..Defects in this system may result in abnormal chromosome segregation and genomic instability, which are characteristic of many cancer cells. ..
  85. Clark P, Jordan F, Pearson C, Walker I, Sattar N, Ellison J, et al. Intercellular adhesion molecule-1 (ICAM-1) expression is upregulated by thrombin in human monocytes and THP-1 cells in vitro and in pregnant subjects in vivo. Thromb Haemost. 2003;89:1043-51 pubmed
  86. Staines A, Sindelar P, Coughtrie M, Burchell B. Farnesol is glucuronidated in human liver, kidney and intestine in vitro, and is a novel substrate for UGT2B7 and UGT1A1. Biochem J. 2004;384:637-45 pubmed
    ..Thus glucuronidation may modulate the physiological and/or pharmacological properties of this potent signalling molecule. ..
  87. Collet B, Collins C. Comparative gene expression profile in two Atlantic salmon cell lines TO and SHK-1. Vet Immunol Immunopathol. 2009;130:92-5 pubmed publisher
    ..The SHK-1 cell line, or a subpopulation of SHK-1 cells, shares characteristics with dendritic cells whilst the TO cell line shows characteristics more traditionally associated with macrophages. ..
  88. Terry S, Riches A, Bryant P. Suppression of topoisomerase IIalpha expression and function in human cells decreases chromosomal radiosensitivity. Mutat Res. 2009;663:40-5 pubmed publisher
  89. Mah L, O Prey J, Baudot A, Hoekstra A, Ryan K. DRAM-1 encodes multiple isoforms that regulate autophagy. Autophagy. 2012;8:18-28 pubmed publisher
    ..In summary, these findings identify new autophagy regulators that provide insight into the control of autophagy downstream of p53. ..
  90. Carlton Smith C, Elliott R. Viperin, MTAP44, and protein kinase R contribute to the interferon-induced inhibition of Bunyamwera Orthobunyavirus replication. J Virol. 2012;86:11548-57 pubmed publisher
    ..Taken together, our data show that the restriction of BUNV replication mediated by IFN is an accumulated effect of at least three IFN-stimulated genes that probably act on different stages of the viral replication cycle. ..
  91. Wu H, Bremner D, Li H, Shi Q, Wu J, Xiao R, et al. A novel multifunctional biomedical material based on polyacrylonitrile: Preparation and characterization. Mater Sci Eng C Mater Biol Appl. 2016;62:702-9 pubmed publisher
    ..This study provides reference data to aid the development of multifunctional textiles and to explore their use in the biomedical material field. ..
  92. Enokido Y, de Sauvage F, Hongo J, Ninkina N, Rosenthal A, Buchman V, et al. GFR alpha-4 and the tyrosine kinase Ret form a functional receptor complex for persephin. Curr Biol. 1998;8:1019-22 pubmed
    ..These results demonstrate that GFR alpha-4 is the ligand-binding subunit for persephin and that persephin, like GDNF and neurturin, also requires Ret for signalling. ..