Experts and Doctors on capsid proteins in New York, United States


Locale: New York, United States
Topic: capsid proteins

Top Publications

  1. Wu B, Chao J, Singer R. Fluorescence fluctuation spectroscopy enables quantitative imaging of single mRNAs in living cells. Biophys J. 2012;102:2936-44 pubmed publisher
    ..This work provides a foundation for quantitative spectroscopy and imaging of single mRNAs directly in live cells. ..
  2. Kelkar S, Pfister K, Crystal R, Leopold P. Cytoplasmic dynein mediates adenovirus binding to microtubules. J Virol. 2004;78:10122-32 pubmed
    ..Partially uncoated capsids isolated from cells 40 min after infection also exhibited microtubule binding. In summary, the primary mode of Ad attachment to microtubules occurs though cytoplasmic dynein-mediated binding. ..
  3. Hueffer K, Truyen U, Parrish C. [Evolution and host variation of the canine parvovirus: molecular basis for the development of a new virus]. Berl Munch Tierarztl Wochenschr. 2004;117:130-5 pubmed
    ..These residues are located on a raised region of the capsid at the three-fold axis of symmetry. Interestingly, adaption of the new virus to the new host appears to correlate with an improved binding to the Tfr receptor. ..
  4. Malik Z, Hailpern S, Burk R. Persistent antibodies to HPV virus-like particles following natural infection are protective against subsequent cervicovaginal infection with related and unrelated HPV. Viral Immunol. 2009;22:445-9 pubmed publisher
  5. Shinozaki K, Suominen E, Carrick F, Sauter B, Kahari V, Lieber A, et al. Efficient infection of tumor endothelial cells by a capsid-modified adenovirus. Gene Ther. 2006;13:52-9 pubmed
    ..Double immunostaining revealed that Ad5/35 colocalized with CD31 and Flk-1 positive endothelial cells. These results indicate that Ad5/35 may be useful in anticancer strategies targeting tumor endothelial cells. ..
  6. Rieder E, Henry T, Duque H, Baxt B. Analysis of a foot-and-mouth disease virus type A24 isolate containing an SGD receptor recognition site in vitro and its pathogenesis in cattle. J Virol. 2005;79:12989-98 pubmed
  7. Song W, Strack R, Jaffrey S. Imaging bacterial protein expression using genetically encoded RNA sensors. Nat Methods. 2013;10:873-5 pubmed publisher
    ..These sensors are key components of a generalizable strategy to image protein expression in a single bacterium in real time. ..
  8. Saiz J, Gonzalez M, Borca M, Sobrino F, Moore D. Identification of neutralizing antigenic sites on VP1 and VP2 of type A5 foot-and-mouth disease virus, defined by neutralization-resistant variants. J Virol. 1991;65:2518-24 pubmed
    ..The relative importance of these two sites in the biological properties of foot-and-mouth disease virus is discussed. ..
  9. Ding R, Medeiros M, Dadhania D, Muthukumar T, Kracker D, Kong J, et al. Noninvasive diagnosis of BK virus nephritis by measurement of messenger RNA for BK virus VP1 in urine. Transplantation. 2002;74:987-94 pubmed
    ..987, <0.00001) for BKV VP1 mRNA levels and 0.562 (95% confidence interval, 0.417 to 0.708, >0.2) for 18S rRNA. Measurement of BKV VP1 mRNA in urinary cells offers a noninvasive and accurate means of diagnosing BKV nephritis. ..

More Information


  1. Briese T, Renwick N, Venter M, Jarman R, Ghosh D, Köndgen S, et al. Global distribution of novel rhinovirus genotype. Emerg Infect Dis. 2008;14:944-7 pubmed publisher
    ..Molecular dating indicates that these viruses have been circulating for at least 250 years. ..
  2. Schoggins J, Falck Pedersen E. Fiber and penton base capsid modifications yield diminished adenovirus type 5 transduction and proinflammatory gene expression with retention of antigen-specific humoral immunity. J Virol. 2006;80:10634-44 pubmed
    ..The fiber-pseudotyped and penton base constructs with RGD deleted have attributes that could be important enhancements in a number of vaccine applications. ..
  3. Kelkar S, De B, Gao G, Wilson J, Crystal R, Leopold P. A common mechanism for cytoplasmic dynein-dependent microtubule binding shared among adeno-associated virus and adenovirus serotypes. J Virol. 2006;80:7781-5 pubmed
    ..Excess AAV2 capsid protein prevented microtubule binding by AAV serotypes 2, 5, and rh.10, as well as adenovirus serotype 5, indicating that similar binding sites are used by these viruses. ..
  4. Kentsis A, Borden K. Physical mechanisms and biological significance of supramolecular protein self-assembly. Curr Protein Pept Sci. 2004;5:125-34 pubmed
  5. Knipe T, Rieder E, Baxt B, Ward G, Mason P. Characterization of synthetic foot-and-mouth disease virus provirions separates acid-mediated disassembly from infectivity. J Virol. 1997;71:2851-6 pubmed
    ..Taken together, these studies are consistent with a role for soluble VP4 molecules in release of the viral genome from the endosomal compartment of susceptible cells. ..
  6. Krause A, Joh J, Hackett N, Roelvink P, Bruder J, Wickham T, et al. Epitopes expressed in different adenovirus capsid proteins induce different levels of epitope-specific immunity. J Virol. 2006;80:5523-30 pubmed
  7. House J, Stott J, Blanchard M, LaRocco M, Llewellyn M. A blocking ELISA for detection of antibody to a subgroup-reactive epitope of African horsesickness viral protein 7 (VP7) using a novel gamma-irradiated antigen. Ann N Y Acad Sci. 1996;791:333-44 pubmed
    ..An ELISA protocol using a blocking format is described. ..
  8. Schoggins J, Falck Pedersen E. Serotype 5 Adenovirus fiber (F7F41S) chimeric vectors incur packaging deficiencies when targeting peptides are inserted into Ad41 short fiber. Virology. 2009;395:10-20 pubmed publisher
  9. Scherer J, Vallee R. Adenovirus recruits dynein by an evolutionary novel mechanism involving direct binding to pH-primed hexon. Viruses. 2011;3:1417-31 pubmed publisher
  10. Thenin Houssier S, de Vera I, Pedro Rosa L, Brady A, Richard A, Konnick B, et al. Ebselen, a Small-Molecule Capsid Inhibitor of HIV-1 Replication. Antimicrob Agents Chemother. 2016;60:2195-208 pubmed publisher
    ..This study reports the use of TR-FRET screening to successfully identify a novel capsid inhibitor, ebselen, validating HIV-1 capsid as a promising target for drug development. ..
  11. Perez Caballero D, Soll S, Bieniasz P. Evidence for restriction of ancient primate gammaretroviruses by APOBEC3 but not TRIM5alpha proteins. PLoS Pathog. 2008;4:e1000181 pubmed publisher
  12. McKinney C, Perez C, Mohr I. Poly(A) binding protein abundance regulates eukaryotic translation initiation factor 4F assembly in human cytomegalovirus-infected cells. Proc Natl Acad Sci U S A. 2012;109:5627-32 pubmed publisher
    ..This finding demonstrates that viruses can increase host translation initiation factor concentration to foster their replication and defines a unique mechanism whereby control of PABP abundance regulates eIF4F assembly. ..
  13. Louis Jeune V, Joergensen J, Hajjar R, Weber T. Pre-existing anti-adeno-associated virus antibodies as a challenge in AAV gene therapy. Hum Gene Ther Methods. 2013;24:59-67 pubmed publisher
    ..In this review, we discuss the current knowledge of the role of anti-AAV antibodies in AAV-based gene therapy with a particular emphasis on approaches to overcome the hurdle that they pose. ..
  14. Billerbeck E, Horwitz J, Labitt R, Donovan B, Vega K, Budell W, et al. Characterization of human antiviral adaptive immune responses during hepatotropic virus infection in HLA-transgenic human immune system mice. J Immunol. 2013;191:1753-64 pubmed publisher
    ..The adenovirus reporter system used in our study may serve as simple in vivo method to evaluate future strategies for improving human intrahepatic immune responses in humanized mice. ..
  15. Jakubiec A, Yang S, Chua N. Arabidopsis DRB4 protein in antiviral defense against Turnip yellow mosaic virus infection. Plant J. 2012;69:14-25 pubmed publisher
    ..Our findings suggest that the control of viral protein as well as RNA levels might be important for mounting an efficient antiviral response...
  16. Liu Y, Wang C, Mueller S, Paul A, Wimmer E, Jiang P. Direct interaction between two viral proteins, the nonstructural protein 2C and the capsid protein VP3, is required for enterovirus morphogenesis. PLoS Pathog. 2010;6:e1001066 pubmed publisher
    ..These conditions lead to morphogenesis at the site where newly synthesized genomes emerge from the replication complex...
  17. Sharma A, Krause A, Xu Y, Sung B, Wu W, Worgall S. Adenovirus-based vaccine with epitopes incorporated in novel fiber sites to induce protective immunity against Pseudomonas aeruginosa. PLoS ONE. 2013;8:e56996 pubmed publisher
    ..F(FG)Epi8 or AdZ.F(HI)Epi8, but not with AdOprF, induced protective immunity against P. aeruginosa. This suggests that incorporation of epitopes into distinct sites of the Ad fiber is a promising vaccine strategy. ..
  18. Shiratsuchi T, Rai U, Krause A, Worgall S, Tsuji M. Replacing adenoviral vector HVR1 with a malaria B cell epitope improves immunogenicity and circumvents preexisting immunity to adenovirus in mice. J Clin Invest. 2010;120:3688-701 pubmed publisher
    ..yoelii CS transgene was maintained. Overall, this study demonstrates that the HVR1-modifed Ad vastly improves upon Ad as a promising malaria vaccine platform candidate...
  19. Quan P, Wagner T, Briese T, Torgerson T, Hornig M, Tashmukhamedova A, et al. Astrovirus encephalitis in boy with X-linked agammaglobulinemia. Emerg Infect Dis. 2010;16:918-25 pubmed publisher
    ..Our findings expand the spectrum of causative agents associated with encephalitis and highlight unbiased molecular technology as a valuable tool for differential diagnosis of unexplained disease...
  20. Lorieau J, Day L, McDermott A. Conformational dynamics of an intact virus: order parameters for the coat protein of Pf1 bacteriophage. Proc Natl Acad Sci U S A. 2008;105:10366-71 pubmed publisher
    ..The large-amplitude dynamic motion of these positively charged side chains in their interactions with the DNA were not previously expected. The results reveal a highly dynamic aspect of a DNA-protein interface within a virus...
  21. Miles L, Burga L, Gardner E, Bostina M, Poirier J, Rudin C. Anthrax toxin receptor 1 is the cellular receptor for Seneca Valley virus. J Clin Invest. 2017;127:2957-2967 pubmed publisher
    ..These studies identify ANTXR1, a class of receptor that is shared by a mammalian virus and a bacterial toxin, as the cellular receptor for SVV. ..
  22. Cimarelli A, Luban J. Translation elongation factor 1-alpha interacts specifically with the human immunodeficiency virus type 1 Gag polyprotein. J Virol. 1999;73:5388-401 pubmed
  23. Tomko R, Johansson C, Totrov M, Abagyan R, Frisen J, Philipson L. Expression of the adenovirus receptor and its interaction with the fiber knob. Exp Cell Res. 2000;255:47-55 pubmed
    ..In fact a specific antibody interfering with only CVB binding recognizes the IG2 domain in the receptor, suggesting that the CVB interacts with this region or an overlap between the IG1 and the IG2 regions. ..
  24. Aguilar P, Leung L, Wang E, Weaver S, Basler C. A five-amino-acid deletion of the eastern equine encephalitis virus capsid protein attenuates replication in mammalian systems but not in mosquito cells. J Virol. 2008;82:6972-83 pubmed publisher
    ..This region is also critical for EEEV pathogenesis. ..
  25. Cheshenko N, Liu W, Satlin L, Herold B. Focal adhesion kinase plays a pivotal role in herpes simplex virus entry. J Biol Chem. 2005;280:31116-25 pubmed
    ..Together, these results support a novel model for invasion that implicates FAK phosphorylation as important for delivery of viral capsids to the nuclear pore. ..
  26. Song B, Spindler K, Young C. Sequence of the mouse adenovirus serotype-1 DNA encoding the precursor to capsid protein VI. Gene. 1995;152:279-80 pubmed
    ..There is a marked conservation at the C terminus, the last eleven residues of which may be necessary for activating the Ad endoproteinase, and at the N terminus, including the consensus endoproteinase cleavage site. ..
  27. Liu D, Day L. Pf1 virus structure: helical coat protein and DNA with paraxial phosphates. Science. 1994;265:671-4 pubmed
  28. Stone Marschat M, Moss S, Burrage T, Barber M, Roy P, Laegreid W. Immunization with VP2 is sufficient for protection against lethal challenge with African horsesickness virus Type 4. Virology. 1996;220:219-22 pubmed
    ..These results demonstrate that AHSV VP2 alone is sufficient to induce a protective immune response in horses and indicate the usefulness of ELISA-reactive antibodies for differentiation of vaccinated and naturally exposed horses...
  29. Graziano V, Luo G, Blainey P, Pérez Berná A, McGrath W, FLINT S, et al. Regulation of a viral proteinase by a peptide and DNA in one-dimensional space: II. adenovirus proteinase is activated in an unusual one-dimensional biochemical reaction. J Biol Chem. 2013;288:2068-80 pubmed publisher
    ..This activation mechanism illustrates a new paradigm for virion maturation and a new way, by sliding on DNA, for bimolecular complexes to form among proteins not involved in DNA metabolism. ..
  30. Wang C, Jiang P, Sand C, Paul A, Wimmer E. Alanine scanning of poliovirus 2CATPase reveals new genetic evidence that capsid protein/2CATPase interactions are essential for morphogenesis. J Virol. 2012;86:9964-75 pubmed publisher
  31. Casas I, Avellon A, Mosquera M, Jabado O, Echevarria J, Campos R, et al. Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region. J Clin Microbiol. 2005;43:6176-82 pubmed
    ..Known associations between specific serotypes and clinical syndromes are verified. Possible new associations are described to allow further independent testing...
  32. Yueh A, Leung J, Bhattacharyya S, Perrone L, De los Santos K, Pu S, et al. Interaction of moloney murine leukemia virus capsid with Ubc9 and PIASy mediates SUMO-1 addition required early in infection. J Virol. 2006;80:342-52 pubmed
    ..The results suggest that the SUMOylation of CA mediated by an interaction with Ubc9 and PIASy is required for early events of infection, after reverse transcription and before nuclear entry and viral DNA integration...
  33. Aguilar P, Weaver S, Basler C. Capsid protein of eastern equine encephalitis virus inhibits host cell gene expression. J Virol. 2007;81:3866-76 pubmed
  34. Bremner K, Scherer J, Yi J, Vershinin M, Gross S, Vallee R. Adenovirus transport via direct interaction of cytoplasmic dynein with the viral capsid hexon subunit. Cell Host Microbe. 2009;6:523-35 pubmed publisher
    ..These results identify hexon as a direct receptor for cytoplasmic dynein and demonstrate that hexon recruits dynein for transport to the nucleus by a mechanism distinct from that for physiological dynein cargo...
  35. Goldbourt A, Gross B, Day L, McDermott A. Filamentous phage studied by magic-angle spinning NMR: resonance assignment and secondary structure of the coat protein in Pf1. J Am Chem Soc. 2007;129:2338-44 pubmed
    ..The chemical shifts of the backbone atoms indicate that the coat protein conformation involves a 40-residue continuous alpha-helix extending from residue 6 to the C-terminus...
  36. Melana S, Nepomnaschy I, Hasa J, Djougarian A, Djougarian A, Holland J, et al. Detection of human mammary tumor virus proteins in human breast cancer cells. J Virol Methods. 2010;163:157-61 pubmed publisher
    ..This should be taken into consideration when MMTV antibodies are used to detect HMTV proteins in human tissues. ..
  37. Auerbach M, Brown K, Singh I. Mutational analysis of the N-terminal domain of Moloney murine leukemia virus capsid protein. J Virol. 2007;81:12337-47 pubmed
    ..Future studies are needed to understand the mechanism of these blocks and to target these regions pharmacologically to inhibit retroviral infection at additional stages...
  38. Leopold P, Crystal R. Intracellular trafficking of adenovirus: many means to many ends. Adv Drug Deliv Rev. 2007;59:810-21 pubmed
    ..This review summarizes the classical adenovirus infection pathway along with the exceptions to that trafficking pathway, providing an overview of intracellular trafficking of adenovirus...
  39. Kratovac Z, Virgen C, Bibollet Ruche F, Hahn B, Bieniasz P, Hatziioannou T. Primate lentivirus capsid sensitivity to TRIM5 proteins. J Virol. 2008;82:6772-7 pubmed publisher
    ..TRIM5alpha proteins from other primates exhibited distinct restriction specificities for primate lentivirus capsids. Finally, we identified novel primate lentiviral capsids that are targeted by TRIMCyp proteins...
  40. Sergeyev I, Day L, Goldbourt A, McDermott A. Chemical shifts for the unusual DNA structure in Pf1 bacteriophage from dynamic-nuclear-polarization-enhanced solid-state NMR spectroscopy. J Am Chem Soc. 2011;133:20208-17 pubmed publisher
    ..Overall, these observations characterize and support the DNA model proposed by Liu and Day and refined by Tsuboi et al., which calls for the most highly stretched and twisted naturally occurring DNA yet encountered...
  41. Grubman M, de Los Santos T. Rapid control of foot-and-mouth disease outbreaks: is RNAi a possible solution?. Trends Immunol. 2005;26:65-8 pubmed
  42. Hatziioannou T, Perez Caballero D, Cowan S, Bieniasz P. Cyclophilin interactions with incoming human immunodeficiency virus type 1 capsids with opposing effects on infectivity in human cells. J Virol. 2005;79:176-83 pubmed
    ..We speculate that CypA and/or CypA-related proteins affect the fate of incoming HIV-1 capsid either directly or by modulating interactions with unidentified host cell factors. ..
  43. Agirrezabala X, Méndez López E, Lasso G, Sánchez Pina M, Aranda M, Valle M. The near-atomic cryoEM structure of a flexible filamentous plant virus shows homology of its coat protein with nucleoproteins of animal viruses. elife. 2015;4:e11795 pubmed publisher
    ..The homology suggests gene transfer between eukaryotic (+) and (-)ssRNA viruses. ..
  44. Graziano V, McGrath W, Suomalainen M, Greber U, Freimuth P, Blainey P, et al. Regulation of a viral proteinase by a peptide and DNA in one-dimensional space: I. binding to DNA AND to hexon of the precursor to protein VI, pVI, of human adenovirus. J Biol Chem. 2013;288:2059-67 pubmed publisher
    ..Three molecules of pVI bound to one molecule of the hexon trimer with an equilibrium dissociation constant K(d)((app)) of 1.1 nm...
  45. Huffman J, Daniel G, Falck Pedersen E, Huet A, Smith G, Conway J, et al. The C Terminus of the Herpes Simplex Virus UL25 Protein Is Required for Release of Viral Genomes from Capsids Bound to Nuclear Pores. J Virol. 2017;91: pubmed publisher
    ..pUL25 is found at each of the capsid vertices as part of the capsid vertex-specific component and implicates the importance of this complex for NPC binding and genome release. ..
  46. Eryilmaz E, Benach J, Su M, Seetharaman J, Dutta K, Wei H, et al. Structure and dynamics of the P7 protein from the bacteriophage phi 12. J Mol Biol. 2008;382:402-22 pubmed publisher
    ..Given the requirement of P7 in maintaining genome packaging efficiency and transcriptional fidelity, our data suggest a central biological role for P7-RNA interactions...
  47. Zheng Y, Kielian M. Imaging of the alphavirus capsid protein during virus replication. J Virol. 2013;87:9579-89 pubmed publisher
    ..Together, our studies provide the first dynamic views of the alphavirus capsid protein in living cells and a system to define detailed mechanisms during alphavirus infection. ..
  48. Bouamr F, Cornilescu C, Goff S, Tjandra N, Carter C. Structural and dynamics studies of the D54A mutant of human T cell leukemia virus-1 capsid protein. J Biol Chem. 2005;280:6792-801 pubmed publisher
  49. Borca M, Pacheco J, Holinka L, Carrillo C, Hartwig E, Garriga D, et al. Role of arginine-56 within the structural protein VP3 of foot-and-mouth disease virus (FMDV) O1 Campos in virus virulence. Virology. 2012;422:37-45 pubmed publisher
    ..O1Ca-VP3-56H was thermo stable and induced typical clinical signs of FMD, whereas O1Ca-VP3-56R presented a ts phenotype and was nonpathogenic unless VP3 position 56 reverted in vivo to either H or cysteine (C)...
  50. Manel N, Hogstad B, Wang Y, Levy D, Unutmaz D, Littman D. A cryptic sensor for HIV-1 activates antiviral innate immunity in dendritic cells. Nature. 2010;467:214-7 pubmed publisher
    ..The virulence of HIV-1 may be related to evasion of this response, the manipulation of which may be necessary to generate an effective HIV-1 vaccine. ..
  51. Sebastian S, Grütter C, Strambio de Castillia C, Pertel T, Olivari S, Grutter M, et al. An invariant surface patch on the TRIM5alpha PRYSPRY domain is required for retroviral restriction but dispensable for capsid binding. J Virol. 2009;83:3365-73 pubmed publisher
    ..These results indicate that, in addition to CA-specific binding, the PRYSPRY domain possesses a second function, possibly binding of a cofactor, that is essential for retroviral restriction activity by TRIM5alpha. ..
  52. Hatziioannou T, Princiotta M, Piatak M, Yuan F, Zhang F, Lifson J, et al. Generation of simian-tropic HIV-1 by restriction factor evasion. Science. 2006;314:95 pubmed
  53. Kim M, Racaniello V. Enterovirus 70 receptor utilization is controlled by capsid residues that also regulate host range and cytopathogenicity. J Virol. 2007;81:8648-55 pubmed
    ..The five EV70 residues define a region of the capsid that controls viral host range, DAF utilization, and cytopathogenicity...