Experts and Doctors on amino acid sequence in Scotland, United Kingdom

Summary

Locale: Scotland, United Kingdom
Topic: amino acid sequence

Top Publications

  1. Mackinnon C, Carter P, Smyth S, Dunbar B, Fothergill J. Molecular cloning of cDNA for human complement component C1s. The complete amino acid sequence. Eur J Biochem. 1987;169:547-53 pubmed
    ..The sequence of the C-terminal chain (251 residues) agrees with that already established to be the "serine protease" domain of C1s. ..
  2. Doza Y, Cuenda A, Thomas G, Cohen P, Nebreda A. Activation of the MAP kinase homologue RK requires the phosphorylation of Thr-180 and Tyr-182 and both residues are phosphorylated in chemically stressed KB cells. FEBS Lett. 1995;364:223-8 pubmed
  3. Cook J, McGregor A, Lee T, Milligan G, Eidne K. A disulfide bonding interaction role for cysteines in the extracellular domain of the thyrotropin-releasing hormone receptor. Endocrinology. 1996;137:2851-8 pubmed
    ..Cys100 is not thought to have a disulfide bonding interaction role. Results obtained after chemical modification have shown that free sulfhydryl groups within the TRH-R may also have a role in ligand interactions. ..
  4. Spence S, Rena G, Sullivan M, Erdogan S, Houslay M. Receptor-mediated stimulation of lipid signalling pathways in CHO cells elicits the rapid transient induction of the PDE1B isoform of Ca2+/calmodulin-stimulated cAMP phosphodiesterase. Biochem J. 1997;321 ( Pt 1):157-63 pubmed
  5. Sullivan M, Rena G, Begg F, Gordon L, Olsen A, Houslay M. Identification and characterization of the human homologue of the short PDE4A cAMP-specific phosphodiesterase RD1 (PDE4A1) by analysis of the human HSPDE4A gene locus located at chromosome 19p13.2. Biochem J. 1998;333 ( Pt 3):693-703 pubmed
  6. Everett R, Meredith M, Orr A. The ability of herpes simplex virus type 1 immediate-early protein Vmw110 to bind to a ubiquitin-specific protease contributes to its roles in the activation of gene expression and stimulation of virus replication. J Virol. 1999;73:417-26 pubmed
    ..The results indicate that the ability to bind to HAUSP contributes to the functional activities of Vmw110. ..
  7. Chamberlain L, Burgoyne R. Cysteine-string protein: the chaperone at the synapse. J Neurochem. 2000;74:1781-9 pubmed
    ..Targets for Csp include the vesicle protein VAMP/synaptobrevin and the plasma membrane protein syntaxin 1, the significance of which is discussed in possible models to account for current knowledge of Csp function. ..
  8. Zhang J, Nicoll G, Jones C, Crocker P. Siglec-9, a novel sialic acid binding member of the immunoglobulin superfamily expressed broadly on human blood leukocytes. J Biol Chem. 2000;275:22121-6 pubmed
    ..These results show that despite their high degree of sequence similarity, Siglec-7 and Siglec-9 have distinct expression profiles. ..
  9. Clucas C, Cabello J, Büssing I, Schnabel R, Johnstone I. Oncogenic potential of a C.elegans cdc25 gene is demonstrated by a gain-of-function allele. EMBO J. 2002;21:665-74 pubmed
    ..1 activity can cause a failure of proliferation of intestinal and other cell types. That gain and loss of CDC-25.1 activity has opposite effects on cellular proliferation indicates its critical role in controlling C.elegans cell number. ..

More Information

Publications192 found, 100 shown here

  1. Biondi R, Komander D, Thomas C, Lizcano J, Deak M, Alessi D, et al. High resolution crystal structure of the human PDK1 catalytic domain defines the regulatory phosphopeptide docking site. EMBO J. 2002;21:4219-28 pubmed
    ..We discuss the possibility that an analogous phosphate-binding regulatory motif may participate in the activation of other AGC kinases. Furthermore, the structure of PDK1 provides a scaffold for the design of specific PDK1 inhibitors. ..
  2. Cutler C, Cramb G. Two isoforms of the Na+/K+/2Cl- cotransporter are expressed in the European eel (Anguilla anguilla). Biochim Biophys Acta. 2002;1566:92-103 pubmed
    ..NKCC1a mRNA was expressed at similar low levels in the middle intestine of FW- and SW-acclimated yellow or silver eels, suggesting the presence of an ion secretory mechanism in this gut segment. ..
  3. Tian L, Coghill L, MacDonald S, Armstrong D, Shipston M. Leucine zipper domain targets cAMP-dependent protein kinase to mammalian BK channels. J Biol Chem. 2003;278:8669-77 pubmed
    ..Thus LZ1 is required for the association and regulation of mammalian BK channels by PKA, and other putative leucine zippers in the BK channel protein may provide anchoring for other regulatory enzyme complexes. ..
  4. Menendez S, Khan Z, Coomber D, Lane D, Higgins M, Koufali M, et al. Oligomerization of the human ARF tumor suppressor and its response to oxidative stress. J Biol Chem. 2003;278:18720-9 pubmed
    ..These observations suggest that p14ARF activity may be linked to its oligomerization status and sensitive to the redox status of the cell. ..
  5. Merckx A, Le Roch K, Nivez M, Dorin D, Alano P, Gutierrez G, et al. Identification and initial characterization of three novel cyclin-related proteins of the human malaria parasite Plasmodium falciparum. J Biol Chem. 2003;278:39839-50 pubmed
    ..This raises the possibility that "cryptic" cell cycle regulators may be found among the 50% of the open reading frames in the P. falciparum genome that display no homology to any known proteins. ..
  6. Milburn C, Boudeau J, Deak M, Alessi D, van Aalten D. Crystal structure of MO25 alpha in complex with the C terminus of the pseudo kinase STE20-related adaptor. Nat Struct Mol Biol. 2004;11:193-200 pubmed
  7. Vickers T, Fairlamb A. Trypanothione S-transferase activity in a trypanosomatid ribosomal elongation factor 1B. J Biol Chem. 2004;279:27246-56 pubmed
    ..Two eEF1Bgamma genes were cloned from C. fasciculata, but recombinant C. fasciculata eEF1Bgamma had no S-transferase activity, suggesting that eEF1Bgamma is unstable in the absence of the other subunits...
  8. Rincon M, Martin J, Aurilia V, McCrae S, Rucklidge G, Reid M, et al. ScaC, an adaptor protein carrying a novel cohesin that expands the dockerin-binding repertoire of the Ruminococcus flavefaciens 17 cellulosome. J Bacteriol. 2004;186:2576-85 pubmed
  9. Packard E, Parton R, Coote J, Fry N. Sequence variation and conservation in virulence-related genes of Bordetella pertussis isolates from the UK. J Med Microbiol. 2004;53:355-65 pubmed publisher
    ..pertussis from different time periods and from different countries. The variation exhibited by prnA and fim2 suggests that they could be useful, additional epidemiological markers in such a typing scheme...
  10. Boulant S, Montserret R, Hope R, Ratinier M, Targett Adams P, Lavergne J, et al. Structural determinants that target the hepatitis C virus core protein to lipid droplets. J Biol Chem. 2006;281:22236-47 pubmed
    ..They also serve as a unique model for elucidating the specificity of protein-lipid interactions between two membrane-bound organelles. ..
  11. Toole B, Cohen P. The skeletal muscle-specific glycogen-targeted protein phosphatase 1 plays a major role in the regulation of glycogen metabolism by adrenaline in vivo. Cell Signal. 2007;19:1044-55 pubmed
  12. Sharma S, Millam S, Hein I, Bryan G. Cloning and molecular characterisation of a potato SERK gene transcriptionally induced during initiation of somatic embryogenesis. Planta. 2008;228:319-30 pubmed publisher
    ..Expression analysis suggests that the isolated StSERK could be a functional SERK orthologue. The possible role of SERK as a marker of pluripotency, rather than embryogenesis alone, is discussed...
  13. Dardente H, Fustin J, Hazlerigg D. Transcriptional feedback loops in the ovine circadian clock. Comp Biochem Physiol A Mol Integr Physiol. 2009;153:391-8 pubmed publisher
    ..Taken together, these data are consistent with a broad conservation of transcriptional and post-translational mechanisms in the circadian clock of diurnal and nocturnal mammals...
  14. Olorunniji F, Stark W. The catalytic residues of Tn3 resolvase. Nucleic Acids Res. 2009;37:7590-602 pubmed publisher
    ..The involvement of some residues in both synapsis and catalysis suggests that they contribute to a regulatory mechanism, in which engagement of catalytic residues with the substrate is coupled to correct assembly of the synapse. ..
  15. Minghetti M, Leaver M, George S. Multiple Cu-ATPase genes are differentially expressed and transcriptionally regulated by Cu exposure in sea bream, Sparus aurata. Aquat Toxicol. 2010;97:23-33 pubmed publisher
    ..Furthermore, our evidence suggests that fish can detoxify excess dietary Cu relatively efficiently but are unable to cope with excess dissolved Cu in the water, demonstrating that the exposure route is critical to toxicity...
  16. Duro E, Lundin C, Ask K, Sanchez Pulido L, MacArtney T, Toth R, et al. Identification of the MMS22L-TONSL complex that promotes homologous recombination. Mol Cell. 2010;40:632-44 pubmed publisher
    ..Therefore, MMS22L and TONSL are required for the maintenance of genome stability when unscheduled DSBs occur in the vicinity of DNA replication forks. ..
  17. Anthony D, Sin Y, Vadrevu S, Advant N, Day J, Byrne A, et al. ?-Arrestin 1 inhibits the GTPase-activating protein function of ARHGAP21, promoting activation of RhoA following angiotensin II type 1A receptor stimulation. Mol Cell Biol. 2011;31:1066-75 pubmed publisher
  18. Monte M, Zou J, Wang T, Carrington A, Secombes C. Cloning, expression analysis and bioactivity studies of rainbow trout (Oncorhynchus mykiss) interleukin-22. Cytokine. 2011;55:62-73 pubmed publisher
    ..The Escherichia coli produced recombinant IL-22 enhanced the expression of a number of antimicrobial peptides, promoting host innate immunity against microbes and revealing a biological similarity with its mammalian counterpart...
  19. Shepherd M, McSorley T, Olsen A, Johnston L, Thomson N, Baillie G, et al. Molecular cloning and subcellular distribution of the novel PDE4B4 cAMP-specific phosphodiesterase isoform. Biochem J. 2003;370:429-38 pubmed
    ..The unique tissue distribution and intracellular targeting of PDE4B4 suggests that this isoform may have a distinct functional role in regulating cAMP levels in specific cell types. ..
  20. Gould G, Holman G. The glucose transporter family: structure, function and tissue-specific expression. Biochem J. 1993;295 ( Pt 2):329-41 pubmed
  21. Hudson P, Rizzoli A, Rosa R, Chemini C, Jones L, Gould E. Tick-borne encephalitis virus in northern Italy: molecular analysis, relationships with density and seasonal dynamics of Ixodes ricinus. Med Vet Entomol. 2001;15:304-13 pubmed
    ..ricinus was observed. This study provides evidence to suggest that roe deer may have an important role to play in the maintenance of tick density and in the persistence of TBE virus. ..
  22. Murray J, Campbell D, Peggie M, Mora A, Alfonso M, Cohen P. Identification of filamin C as a new physiological substrate of PKBalpha using KESTREL. Biochem J. 2004;384:489-94 pubmed
    ..These results identify the muscle-specific isoform FLNc as a new physiological substrate for PKB. ..
  23. Jaleel M, McBride A, Lizcano J, Deak M, Toth R, Morrice N, et al. Identification of the sucrose non-fermenting related kinase SNRK, as a novel LKB1 substrate. FEBS Lett. 2005;579:1417-23 pubmed
    ..These results provide evidence that SNRK could mediate some of the physiological effects of LKB1. ..
  24. Balendran A, Currie R, Armstrong C, Avruch J, Alessi D. Evidence that 3-phosphoinositide-dependent protein kinase-1 mediates phosphorylation of p70 S6 kinase in vivo at Thr-412 as well as Thr-252. J Biol Chem. 1999;274:37400-6 pubmed
    ..These observations indicate that PDK1 regulates the activation of p70 S6 kinase and provides evidence that PDK1 mediates the phosphorylation of p70 S6 kinase at Thr-412. ..
  25. McEwan I, McGuinness D, Hay C, Millar R, Saunders P, Fraser H. Identification of androgen receptor phosphorylation in the primate ovary in vivo. Reproduction. 2010;140:93-104 pubmed publisher
    ..These results demonstrate that phosphorylation of the AR occurs, at a subset of serine residues, in a reproductive target tissue in vivo, which appears refractory to hormonal manipulations. ..
  26. Li R, Dooley H, Wang T, Secombes C, Bird S. Characterisation and expression analysis of B-cell activating factor (BAFF) in spiny dogfish (Squalus acanthias): cartilaginous fish BAFF has a unique extra exon that may impact receptor binding. Dev Comp Immunol. 2012;36:707-17 pubmed publisher
    ..The dogfish BAFF is highly expressed in immune tissues, such as spleen, and is up-regulated by PWM in peripheral blood leucocytes, suggesting a potentially important role in the immune system...
  27. Dombradi V, Axton J, Glover D, Cohen P. Molecular cloning and chromosomal localization of a novel Drosophila protein phosphatase. FEBS Lett. 1989;247:391-5 pubmed
    ..These and other comparisons demonstrate that this novel enzyme is not the Drosophila counterpart of mammalian protein phosphatases 1, 2A, 2B, 2C or X. ..
  28. Barber M, Travers M. Elucidation of a promoter activity that directs the expression of acetyl-CoA carboxylase alpha with an alternative N-terminus in a tissue-restricted fashion. Biochem J. 1998;333 ( Pt 1):17-25 pubmed
    ..Promoter activity is also evident in primary sheep mammary epithelial cells. These results demonstrate the presence of a third promoter, PIII, in the ACC-alpha gene that results in the tissue-restricted expression of an ACC isoenzyme. ..
  29. Carter P, Duponchel C, Tosi M, Fothergill J. Complete nucleotide sequence of the gene for human C1 inhibitor with an unusually high density of Alu elements. Eur J Biochem. 1991;197:301-8 pubmed
    ..There is no obvious promoter site of the TATA-box type at the 5' end of the gene, but instead it contains a region of potential H-DNA structure similar to that found upstream of the human c-myc gene. ..
  30. Winter A, Myllyharju J, Page A. A hypodermally expressed prolyl 4-hydroxylase from the filarial nematode Brugia malayi is soluble and active in the absence of protein disulfide isomerase. J Biol Chem. 2003;278:2554-62 pubmed
    ..elegans. Although active by itself, the B. malayi PHY-1 was not able to replace enzyme function in a C. elegans P4H mutant...
  31. Roberts C, Roberts F, Henriquez F, Akiyoshi D, Samuel B, Richards T, et al. Evidence for mitochondrial-derived alternative oxidase in the apicomplexan parasite Cryptosporidium parvum: a potential anti-microbial agent target. Int J Parasitol. 2004;34:297-308 pubmed
    ..These results give insight into the evolution of AOX and demonstrate the potential of targeting the alternative pathway of respiration in apicomplexans. ..
  32. Temple G, Cole N, Johnston I. Embryonic temperature and the relative timing of muscle-specific genes during development in herring (Clupea harengus L.). J Exp Biol. 2001;204:3629-37 pubmed
    ..We suggest that the effects of temperature on myofibril synthesis must occur downstream from MyHC transcription either at the level of translation or at the assembly stage. ..
  33. McCoy C, Macdonald A, Morrice N, Campbell D, Deak M, Toth R, et al. Identification of novel phosphorylation sites in MSK1 by precursor ion scanning MS. Biochem J. 2007;402:491-501 pubmed
  34. Morton S, Hesson L, Peggie M, Cohen P. Enhanced binding of TBK1 by an optineurin mutant that causes a familial form of primary open angle glaucoma. FEBS Lett. 2008;582:997-1002 pubmed publisher
    ..The OPTN[E50K] mutant associated with Primary Open Angle Glaucoma (POAG) displayed strikingly enhanced binding to TBK1, suggesting that this interaction may contribute to familial POAG caused by this mutation. ..
  35. Rena G, Guo S, Cichy S, Unterman T, Cohen P. Phosphorylation of the transcription factor forkhead family member FKHR by protein kinase B. J Biol Chem. 1999;274:17179-83 pubmed
    ..The conservation of Thr-24, Ser-256, and Ser-319 and the sequences surrounding them in DAF16 therefore suggests that DAF16 is also a direct substrate for PKB in C. elegans. ..
  36. Pettitt J, Cox E, Broadbent I, Flett A, Hardin J. The Caenorhabditis elegans p120 catenin homologue, JAC-1, modulates cadherin-catenin function during epidermal morphogenesis. J Cell Biol. 2003;162:15-22 pubmed
    ..These results suggest that JAC-1 acts as a positive modulator of cadherin function in C. elegans. ..
  37. Millar R, Lu Z, Pawson A, Flanagan C, Morgan K, Maudsley S. Gonadotropin-releasing hormone receptors. Endocr Rev. 2004;25:235-75 pubmed
    ..Delineation of the structural elements in GnRH and the receptor, which facilitate differential signaling, will contribute to the development of novel interventive GnRH analogs. ..
  38. Wilson L, Reyns G, Darras V, Coughtrie M. cDNA cloning, functional expression, and characterization of chicken sulfotransferases belonging to the SULT1B and SULT1C families. Arch Biochem Biophys. 2004;428:64-72 pubmed
    ..This is the first detailed characterization of sulfotransferases from the chicken, and it demonstrates that the avian and mammalian SULT1 enzymes are closely related in both structure and function. ..
  39. Maslon M, Hrstka R, Vojtesek B, Hupp T. A divergent substrate-binding loop within the pro-oncogenic protein anterior gradient-2 forms a docking site for Reptin. J Mol Biol. 2010;404:418-38 pubmed publisher
  40. Gordon C, McGurk G, Wallace M, Hastie N. A conditional lethal mutant in the fission yeast 26 S protease subunit mts3+ is defective in metaphase to anaphase transition. J Biol Chem. 1996;271:5704-11 pubmed
    ..The deduced amino acid sequences of both mts3+ and NIN1 show homology to peptide sequences obtained from subunit 14 of the 26 S protease purified from bovine or human cells. ..
  41. Nisbet A, Knox D, McNair C, Meikle L, Smith S, Wildblood L, et al. Immune recognition of the surface associated antigen, Tc-SAA-1, from infective larvae of Teladorsagia circumcincta. Parasite Immunol. 2009;31:32-40 pubmed publisher
    ..IgA in abomasal mucus derived from sheep that had received a trickle infection of T. circumcincta bound recombinant Tc-SAA-1. ..
  42. Ninkina N, Mertsalov I, Kulikova D, Alimova Kost M, Simonova O, Korochkin L, et al. Cerd4, third member of the d4 gene family: expression and organization of genomic locus. Mamm Genome. 2001;12:862-6 pubmed
    ..The intron/exon structure of human Cerd4 gene is similar to that of neuro-d4 and ubi-d4/Requiem genes, but most introns of Cerd4 gene are much larger than the corresponding introns of the other two genes. ..
  43. Hussey A, Hayes J. Characterization of a human class-Theta glutathione S-transferase with activity towards 1-menaphthyl sulphate. Biochem J. 1992;286 ( Pt 3):929-35 pubmed
  44. Deka R, Kleanthous C, Coggins J. Identification of the essential histidine residue at the active site of Escherichia coli dehydroquinase. J Biol Chem. 1992;267:22237-42 pubmed
    ..We propose that His-143 is the active site histidine responsible for DEP-mediated inactivation of dehydroquinase and is a good candidate for the general base that has been postulated to participate in the mechanism of this enzyme. ..
  45. Ali S, Clark A. Characterization of the gene encoding ovine beta-lactoglobulin. Similarity to the genes for retinol binding protein and other secretory proteins. J Mol Biol. 1988;199:415-26 pubmed
    ..These proteins are all members of a large, diverse family of secretory proteins, many of which function in binding small hydrophobic molecules. ..
  46. Hayes J, Judah D, Neal G. Resistance to aflatoxin B1 is associated with the expression of a novel aldo-keto reductase which has catalytic activity towards a cytotoxic aldehyde-containing metabolite of the toxin. Cancer Res. 1993;53:3887-94 pubmed
    ..The relative importance of AFB1-AR and the glutathione-S-transferase Yc2 subunit in conferring resistance to aflatoxin B1 is discussed. ..
  47. Mackintosh C, Meek S. Regulation of plant NR activity by reversible phosphorylation, 14-3-3 proteins and proteolysis. Cell Mol Life Sci. 2001;58:205-14 pubmed
    ..Surprisingly, the question of how changes in NR activity actually affect the rate of nitrate assimilation is turning out to be a tough problem. ..
  48. White M, Fothergill Gilmore L. Sequence of the gene encoding phosphoglycerate mutase from Saccharomyces cerevisiae. FEBS Lett. 1988;229:383-7 pubmed
    ..The sequence shows a strong codon bias. The upstream untranslated portion of the gene contains a CT-rich block such as is found in many highly expressed yeast genes, but does not have the associated CAAG sequence. ..
  49. Alexeev D, Bury S, Boys C, Turner M, Sawyer L, Ramsey A, et al. Sequence and crystallization of Escherichia coli dethiobiotin synthetase, the penultimate enzyme of biotin biosynthesis. J Mol Biol. 1994;235:774-6 pubmed
    ..The cell dimensions are a = 72.8 A, b = 49.2 A, c = 61.4 A, beta = 106.2 degrees. The systematic absences are consistent with the monoclinic space group C2 with one polypeptide chain in the asymmetric unit. ..
  50. Cheung P, Trinkle Mulcahy L, Cohen P, Lucocq J. Characterization of a novel phosphatidylinositol 3-phosphate-binding protein containing two FYVE fingers in tandem that is targeted to the Golgi. Biochem J. 2001;355:113-21 pubmed
    ..Cell localization studies using a green fluorescent protein fusion show that TAFF1 is localized to the Golgi, and that the Golgi targeting sequence is located within the N-terminal 187 residues and not in either FYVE domain. ..
  51. Klejnot M, Kozielski F. Structural insights into human Kif7, a kinesin involved in Hedgehog signalling. Acta Crystallogr D Biol Crystallogr. 2012;68:154-9 pubmed publisher
    ..These data are a first step towards structural characterization of a kinesin-4 family member and of this interesting molecular motor of medical significance. ..
  52. Lizcano J, Alrubaie S, Kieloch A, Deak M, Leevers S, Alessi D. Insulin-induced Drosophila S6 kinase activation requires phosphoinositide 3-kinase and protein kinase B. Biochem J. 2003;374:297-306 pubmed
    ..The results of the present study support the view that, in Drosophila cells, dPI3K and dPKB, as well dTOR, are required for the activation of dS6K by insulin. ..
  53. Zeqiraj E, Filippi B, Deak M, Alessi D, van Aalten D. Structure of the LKB1-STRAD-MO25 complex reveals an allosteric mechanism of kinase activation. Science. 2009;326:1707-11 pubmed publisher
    ..The structure also reveals how mutations found in Peutz-Jeghers syndrome and in various sporadic cancers impair LKB1 function. ..
  54. Xu Z, Ogawa H, Vagnarelli P, Bergmann J, Hudson D, Ruchaud S, et al. INCENP-aurora B interactions modulate kinase activity and chromosome passenger complex localization. J Cell Biol. 2009;187:637-53 pubmed publisher
    ..These experiments reveal that INCENP interactions with aurora B in vivo modulate the level of kinase activity, thus regulating CPC localization and functions during mitosis. ..
  55. Moynie L, Schnell R, McMahon S, Sandalova T, Boulkerou W, Schmidberger J, et al. The AEROPATH project targeting Pseudomonas aeruginosa: crystallographic studies for assessment of potential targets in early-stage drug discovery. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013;69:25-34 pubmed publisher
    ..The structural information is expected to provide a firm basis for the improvement of hit compounds identified from fragment-based and high-throughput screening campaigns. ..
  56. Klejnot M, Gabrielsen M, Cameron J, Mleczak A, Talapatra S, Kozielski F, et al. Analysis of the human cofilin 1 structure reveals conformational changes required for actin binding. Acta Crystallogr D Biol Crystallogr. 2013;69:1780-8 pubmed publisher
    ..8?Å resolution. These studies revealed how the actin-binding helix undergoes a conformational change that increases the number of potential hydrogen bonds available for substrate binding. ..
  57. Armstrong C, Doherty M, Cohen P. Identification of the separate domains in the hepatic glycogen-targeting subunit of protein phosphatase 1 that interact with phosphorylase a, glycogen and protein phosphatase 1. Biochem J. 1998;336 ( Pt 3):699-704 pubmed
    ..16, 1876-1887] located at residues 61-64 of GL and preceding lysine residues at positions 56, 57 and 59. A possible approach for increasing glycogen synthesis in certain disorders is discussed. ..
  58. Najafov A, Shpiro N, Alessi D. Akt is efficiently activated by PIF-pocket- and PtdIns(3,4,5)P3-dependent mechanisms leading to resistance to PDK1 inhibitors. Biochem J. 2012;448:285-95 pubmed publisher
    ..Our results suggest further work is warranted to explore the utility of combining PDK1 and mTOR inhibitors as a therapeutic strategy for treatment of cancers that harbour mutations elevating Akt activity. ..
  59. Jedlitschky G, Cassidy A, Sales M, Pratt N, Burchell B. Cloning and characterization of a novel human olfactory UDP-glucuronosyltransferase. Biochem J. 1999;340 ( Pt 3):837-43 pubmed
    ..Furthermore, some steroids, especially androgens, some drugs and carcinogens were conjugated. The results support a role of the enzyme in olfactory perception and in protection of the neural system against airborne hazardous chemicals. ..
  60. Dowler S, Currie R, Downes C, Alessi D. DAPP1: a dual adaptor for phosphotyrosine and 3-phosphoinositides. Biochem J. 1999;342 ( Pt 1):7-12 pubmed
  61. Stewart M, MacKay N, Hanlon L, Blyth K, Scobie L, Cameron E, et al. Insertional mutagenesis reveals progression genes and checkpoints in MYC/Runx2 lymphomas. Cancer Res. 2007;67:5126-33 pubmed
  62. Rao V, Shepherd S, Owen R, Hunter W. Structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013;69:243-7 pubmed publisher
    ..Specific differences that have been proven to contribute to selectivity against the human enzyme in a study of Cryptosporidium parvum IMPDH are also conserved, highlighting the potential value of IMPDH as a drug target. ..
  63. Nicoll G, Ni J, Liu D, Klenerman P, Munday J, Dubock S, et al. Identification and characterization of a novel siglec, siglec-7, expressed by human natural killer cells and monocytes. J Biol Chem. 1999;274:34089-95 pubmed
    ..Immunoprecipitation experiments indicated that siglec-7 is expressed as a monomer of approximately 65 kDa. ..
  64. Rena G, Begg F, Ross A, MacKenzie C, McPhee I, Campbell L, et al. Molecular cloning, genomic positioning, promoter identification, and characterization of the novel cyclic amp-specific phosphodiesterase PDE4A10. Mol Pharmacol. 2001;59:996-1011 pubmed
    ..We suggest that the unique N-terminal regions of PDE4A isoforms confer distinct properties upon them. ..
  65. Gare D, Boyd J, Connolly B. Developmental regulation and secretion of nematode-specific cysteine-glycine domain proteins in Trichinella spiralis. Mol Biochem Parasitol. 2004;134:257-66 pubmed
    ..Both predicted proteins contain an N-terminal signal peptide and we subsequently confirmed by MALDI-TOF mass spectrometric analyses of excretory/secretory peptide spots excised from two-dimensional gels that Ts-CCG-2 is secreted. ..
  66. Clifton A, Young P, Cohen P. A comparison of the substrate specificity of MAPKAP kinase-2 and MAPKAP kinase-3 and their activation by cytokines and cellular stress. FEBS Lett. 1996;392:209-14 pubmed
    ..These results establish that MAPKAP kinase-3 lies 'downstream' of SAPK-2 and that it is likely to have overlapping or identical substrates to MAPKAP kinase-2 in vivo. ..
  67. Fyfe P, Dawson A, Hutchison M, Cameron S, Hunter W. Structure of Staphylococcus aureus adenylosuccinate lyase (PurB) and assessment of its potential as a target for structure-based inhibitor discovery. Acta Crystallogr D Biol Crystallogr. 2010;66:881-8 pubmed publisher
    ..The distinct subunit-subunit interfaces may provide a potential area to target by exploiting the observation that creation of the enzyme active site is dependent on oligomerization. ..
  68. Bacharach E, Mishra N, Briese T, Zody M, Kembou Tsofack J, Zamostiano R, et al. Characterization of a Novel Orthomyxo-like Virus Causing Mass Die-Offs of Tilapia. MBio. 2016;7:e00431-16 pubmed publisher
    ..We also describe complete genomic and protein sequences that will facilitate TiLV detection and containment and enable vaccine development. ..
  69. Kelly V, Sherratt P, Crouch D, Hayes J. Novel homodimeric and heterodimeric rat gamma-hydroxybutyrate synthases that associate with the Golgi apparatus define a distinct subclass of aldo-keto reductase 7 family proteins. Biochem J. 2002;366:847-61 pubmed
  70. Caporale M, Arnaud F, Mura M, Golder M, Murgia C, Palmarini M. The signal peptide of a simple retrovirus envelope functions as a posttranscriptional regulator of viral gene expression. J Virol. 2009;83:4591-604 pubmed publisher
    ..JSE-SP is a multifunctional protein, as it moderately enhances nuclear export of unspliced viral mRNA and considerably increases viral particle release by favoring a posttranslational step of the replication cycle...
  71. Dzamko N, Deak M, Hentati F, Reith A, Prescott A, Alessi D, et al. Inhibition of LRRK2 kinase activity leads to dephosphorylation of Ser(910)/Ser(935), disruption of 14-3-3 binding and altered cytoplasmic localization. Biochem J. 2010;430:405-13 pubmed publisher
    ..They will also stimulate further research to understand how phosphorylation of Ser910 and Ser935 is controlled by LRRK2, and establish any relationship to development of Parkinson's disease. ..
  72. Roff M, Thompson J, Rodriguez M, Jacque J, Baleux F, Arenzana Seisdedos F, et al. Role of IkappaBalpha ubiquitination in signal-induced activation of NFkappaB in vivo. J Biol Chem. 1996;271:7844-50 pubmed
    ..Thus signal-induced activation of NF-kappaB involves phosphorylation-dependent ubiquitination of IkappaBalpha, which targets the protein for rapid degradation by the proteasome and releases NF-kappaB for translocation to the nucleus. ..
  73. Dardente H, Birnie M, Lincoln G, Hazlerigg D. RFamide-related peptide and its cognate receptor in the sheep: cDNA cloning, mRNA distribution in the hypothalamus and the effect of photoperiod. J Neuroendocrinol. 2008;20:1252-9 pubmed publisher
    ..Taken together, these data strengthen the emerging view that interplay between ependymal cells and the pars tuberalis might be important for the seasonal timing system...
  74. Zdarsky E, Favor J, Jackson I. The molecular basis of brown, an old mouse mutation, and of an induced revertant to wild type. Genetics. 1990;126:443-9 pubmed
    ..The sequence of the revertant is the first report of DNA sequence of an ethylnitrosourea-induced genetic change in mouse. ..
  75. Kobayashi T, Cohen P. Activation of serum- and glucocorticoid-regulated protein kinase by agonists that activate phosphatidylinositide 3-kinase is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2. Biochem J. 1999;339 ( Pt 2):319-28 pubmed
    ..These findings raise the possibility that some physiological roles ascribed to PKB on the basis of the overexpression of constitutively active PKB mutants might be mediated by SGK. ..
  76. Murray J, Campbell D, Morrice N, Auld G, Shpiro N, Marquez R, et al. Exploitation of KESTREL to identify NDRG family members as physiological substrates for SGK1 and GSK3. Biochem J. 2004;384:477-88 pubmed
    ..Our results identify NDRG1 and NDRG2 as physiological substrates for SGK1, and demonstrate that phosphorylation of NDRG1 by SGK1 primes it for phosphorylation by GSK3. ..
  77. Rousseau S, Peggie M, Campbell D, Nebreda A, Cohen P. Nogo-B is a new physiological substrate for MAPKAP-K2. Biochem J. 2005;391:433-40 pubmed
    ..The anisomycin-induced phosphorylation of Ser107 in HeLa cells can be prevented by 'knockdown' of MAPKAP-K2 using siRNA (small interfering RNA). Taken together, our results identify Nogo-B as a new physiological substrate of MAPKAP-K2. ..
  78. Robinson D, Stewart K, Price N, Chalk P, Coggins J, Lapthorn A. Crystal structures of Helicobacter pylori type II dehydroquinase inhibitor complexes: new directions for inhibitor design. J Med Chem. 2006;49:1282-90 pubmed publisher
  79. Vouyiouklis D, Werner H, Griffiths I, Stewart G, Armin Nave K, Thomson C. Molecular cloning and transfection studies of M6b-2, a novel splice variant of a member of the PLP-DM20/M6 gene family. J Neurosci Res. 1998;52:633-40 pubmed
    ..It is therefore possible that the function(s) of M6b-2 may be regulated intracellularly by phosphorylation during CNS development. ..
  80. Giraud M, Leonard G, Field R, Berlind C, Naismith J. RmlC, the third enzyme of dTDP-L-rhamnose pathway, is a new class of epimerase. Nat Struct Biol. 2000;7:398-402 pubmed
    ..Sequence alignments of other RmlC enzymes confirm that this region is very highly conserved. The enzyme is distinct structurally from other epimerases known and thus, is the first example of a new class of carbohydrate epimerase. ..
  81. Walden H, Bell G, Russell R, Siebers B, Hensel R, Taylor G. Tiny TIM: a small, tetrameric, hyperthermostable triosephosphate isomerase. J Mol Biol. 2001;306:745-57 pubmed publisher
    ..The PwTIM seems to have removed these regions of potential instability in the formation of the tetramer. This study of PwTIM provides further support for the role of higher oligomerisation states in extreme thermal stabilisation...
  82. Brown T, Mistry R, Gray R, Imrie M, Collie D, Sallenave J. Characterization of the ovine ortholog of secretory leukoprotease inhibitor. Mamm Genome. 2005;16:621-30 pubmed
    ..In summary, the ovine ortholog of SLPI shows similarities to other members of the SLPI family and has all the features of a modulator of innate immunity...
  83. Meng D, Lynch M, Huston E, Beyermann M, Eichhorst J, Adams D, et al. MEK1 binds directly to betaarrestin1, influencing both its phosphorylation by ERK and the timing of its isoprenaline-stimulated internalization. J Biol Chem. 2009;284:11425-35 pubmed publisher
    ..In addition, the MEK disruptor peptide promoted the ability of betaarrestin1 to co-immunoprecipitate with endogenous c-Src and clathrin, facilitating the isoprenaline-stimulated internalization of the beta(2)-adrenergic receptor. ..
  84. Wawra S, Agacan M, Boddey J, Davidson I, Gachon C, Zanda M, et al. Avirulence protein 3a (AVR3a) from the potato pathogen Phytophthora infestans forms homodimers through its predicted translocation region and does not specifically bind phospholipids. J Biol Chem. 2012;287:38101-9 pubmed publisher
    ..However, we show that the observed phospholipid interaction is attributable to a weak association with denatured protein molecules and is therefore most likely physiologically irrelevant...
  85. Gould G, Brant A, Kahn B, Shepherd P, McCoid S, Gibbs E. Expression of the brain-type glucose transporter is restricted to brain and neuronal cells in mice. Diabetologia. 1992;35:304-9 pubmed
  86. Armstrong C, Browne G, Cohen P, Cohen P. PPP1R6, a novel member of the family of glycogen-targetting subunits of protein phosphatase 1. FEBS Lett. 1997;418:210-4 pubmed
    ..The PPP1R6 is associated with glycogen in muscle but is not subject to the same modes of covalent and allosteric regulation as G(M) and G(L). ..
  87. Rembutsu M, Soutar M, van Aalten L, Gourlay R, Hastie C, McLauchlan H, et al. Novel procedure to investigate the effect of phosphorylation on protein complex formation in vitro and in cells. Biochemistry. 2008;47:2153-61 pubmed publisher
    ..Finally, we used this approach to identify the mitochondrial protein ANT as a novel CRMP2 and CRMP4 binding protein. We believe that this approach could be applied generally to the study of phosphorylation-dependent interactions. ..
  88. Alberdi M, Watson E, McAllister G, Harris J, Paxton E, Thomson J, et al. Expression by Lawsonia intracellularis of type III secretion system components during infection. Vet Microbiol. 2009;139:298-303 pubmed publisher
    ..By analogy to other bacteria, this T3SS may be crucial for intracellular development and is likely to play a significant role in the virulence of this unusual pathogen...
  89. Sapkota G, Boudeau J, Deak M, Kieloch A, Morrice N, Alessi D. Identification and characterization of four novel phosphorylation sites (Ser31, Ser325, Thr336 and Thr366) on LKB1/STK11, the protein kinase mutated in Peutz-Jeghers cancer syndrome. Biochem J. 2002;362:481-90 pubmed
  90. D az Rosales P, Bird S, Wang T, Fujiki K, Davidson W, Zou J, et al. Rainbow trout interleukin-2: cloning, expression and bioactivity analysis. Fish Shellfish Immunol. 2009;27:414-22 pubmed publisher
    ..The potential signalling pathways involved and possible use as an adjuvant for fish vaccines are discussed...
  91. Monroig O, Zheng X, Morais S, Leaver M, Taggart J, Tocher D. Multiple genes for functional 6 fatty acyl desaturases (Fad) in Atlantic salmon (Salmo salar L.): gene and cDNA characterization, functional expression, tissue distribution and nutritional regulation. Biochim Biophys Acta. 2010;1801:1072-81 pubmed publisher
    ..The observed differences in tissue expression and nutritional regulation of the fad genes are discussed in relation to gene structures and fish physiology. ..
  92. Philp A, Axton J, Saunders R, Glover D. Mutations in the Drosophila melanogaster gene three rows permit aspects of mitosis to continue in the absence of chromatid segregation. J Cell Sci. 1993;106 ( Pt 1):87-98 pubmed
    ..Chromosomes in the cells of thr embryos do not undertake anaphase separation, but remain at the metaphase plate. Subsequently they decondense. A subset of nuclei go on to replicate their DNA but there is no further mitotic division. ..