Genomes and Genes
Experts and Doctors on adenosine triphosphate in New York, United States
Locale: New York, United States
Topic: adenosine triphosphate
Publications254 found, 100 shown here
- Liu Q, Hendrickson W. Insights into Hsp70 chaperone activity from a crystal structure of the yeast Hsp110 Sse1. Cell. 2007;131:106-20 pubmed..An atomic-level understanding of Hsp70 communication between ATP and substrate-binding domains follows. Requirements on Sse1 for yeast viability are in keeping with the distinct function of Hsp110s as nucleotide exchange factors. ..
- Rosenfeld S, van Duffelen M, Behnke Parks W, Beadle C, Corrreia J, Xing J. The ATPase cycle of the mitotic motor CENP-E. J Biol Chem. 2009;284:32858-68 pubmed publisher..These results suggest that like kinesin 1, features outside of the catalytic domain of CENP-E may also play a role in regulating the processive behavior of this motor. ..
- Escobar Alvarez S, Gardner J, Sheth A, Manfredi G, Yang G, Ouerfelli O, et al. Inhibition of human peptide deformylase disrupts mitochondrial function. Mol Cell Biol. 2010;30:5099-109 pubmed publisher..Therefore, HsPDF appears to have a role in maintenance of mitochondrial respiratory function, and this function is analogous to that of chloroplast PDF. ..
- Khleborodova A, Pan X, Nagre N, Ryan K. An investigation into the role of ATP in the mammalian pre-mRNA 3' cleavage reaction. Biochimie. 2016;125:213-22 pubmed publisher..Fluctuating ATP levels might contribute to the regulation of pre-mRNA 3' cleavage, but the three subunits investigated here do not appear to be responsible for the ATP-stimulation of pre-mRNA cleavage. ..
- Claeys Bouuaert C, Keeney S. Distinct DNA-binding surfaces in the ATPase and linker domains of MutL? determine its substrate specificities and exert separable functions in meiotic recombination and mismatch repair. PLoS Genet. 2017;13:e1006722 pubmed publisher..Taken together, our results offer insights into the structure-function relationships of the MutL? complex and reveal unanticipated genetic relationships between components of the meiotic recombination machinery. ..
- Sung P, Watkins J, Prakash L, Prakash S. Negative superhelicity promotes ATP-dependent binding of yeast RAD3 protein to ultraviolet-damaged DNA. J Biol Chem. 1994;269:8303-8 pubmed..The rad3 Arg-48 mutant protein, which lacks the DNA helicase activity, also binds UV-damaged DNA preferentially, indicating that DNA helicase and damage binding are two distinct and separable functional entities in RAD3. ..
- Mohammadi M, Schlessinger J, Hubbard S. Structure of the FGF receptor tyrosine kinase domain reveals a novel autoinhibitory mechanism. Cell. 1996;86:577-87 pubmed..Finally, the structure provides a basis for rationalizing the effects of kinase mutations in FGF receptors that lead to developmental disorders in nematodes and humans. ..
- Gajewski C, Yang L, Schon E, Manfredi G. New insights into the bioenergetics of mitochondrial disorders using intracellular ATP reporters. Mol Biol Cell. 2003;14:3628-35 pubmed..The severe decrease in nuclear ATP content under "OXPHOS-only" conditions implies that depletion of nuclear ATP plays an important, and hitherto unappreciated, role in patients with mitochondrial dysfunction. ..
- Lavy T, Kumar P, He H, Joshua Tor L. The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation. Genes Dev. 2012;26:294-303 pubmed publisher..The structure of the complex provides a rationale for the phenotypes of several well-known Gal80p and Gal3p mutants as well as the lack of galactokinase activity of Gal3p. ..
- Vivanco I, Robins H, Rohle D, Campos C, Grommes C, Nghiemphu P, et al. Differential sensitivity of glioma- versus lung cancer-specific EGFR mutations to EGFR kinase inhibitors. Cancer Discov. 2012;2:458-71 pubmed publisher..Here, we demonstrate selective sensitivity of glioma-specific EGFR mutants to ATP-site competitive EGFR kinase inhibitors that target the inactive conformation of the catalytic domain. ..
- Shibahara K, Stillman B. Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. Cell. 1999;96:575-85 pubmed..We suggest that PCNA and CAF-1 connect DNA replication to chromatin assembly and the inheritance of epigenetic chromosome states. ..
- CSANADY L, Chan K, Seto Young D, Kopsco D, Nairn A, Gadsby D. Severed channels probe regulation of gating of cystic fibrosis transmembrane conductance regulator by its cytoplasmic domains. J Gen Physiol. 2000;116:477-500 pubmed..As the maximal opening rates were the same for WT channels and split channels with no R domain, it seems that the phosphorylated R domain does not stimulate opening of CFTR channels; rather, the dephosphorylated R domain inhibits them. ..
- Pallotti F, Baracca A, Hernandez Rosa E, Walker W, Solaini G, Lenaz G, et al. Biochemical analysis of respiratory function in cybrid cell lines harbouring mitochondrial DNA mutations. Biochem J. 2004;384:287-93 pubmed..However, the distinct clinical features associated with some of these mutations still remain to be elucidated. ..
- Tanaka N, Schwer B. Mutations in PRP43 that uncouple RNA-dependent NTPase activity and pre-mRNA splicing function. Biochemistry. 2006;45:6510-21 pubmed..Optimal ATP hydrolysis requires an RNA cofactor of >or=20 nt. Prp43 is largely indifferent to mutations in its C-terminal segment, which is conserved in the DEAH-box splicing factors Prp2, Prp16, and Prp22. ..
- Keppetipola N, Jain R, Shuman S. Novel triphosphate phosphohydrolase activity of Clostridium thermocellum TTM, a member of the triphosphate tunnel metalloenzyme superfamily. J Biol Chem. 2007;282:11941-9 pubmed publisher..Our results underscore the plasticity of CthTTM substrate choice and suggest how novel specificities within the TTM superfamily might evolve through changes in the residues that line the tunnel walls...
- Vives Bauza C, Anand M, Shiraz A, Shirazi A, Magrane J, Gao J, et al. The age lipid A2E and mitochondrial dysfunction synergistically impair phagocytosis by retinal pigment epithelial cells. J Biol Chem. 2008;283:24770-80 pubmed publisher..They suggest that synergy of sub-toxic lysosomal and mitochondrial changes in RPE cells with age may cause RPE dysfunction that is known to contribute to human retinal diseases like age-related macular degeneration. ..
- Zhao Z, Zhao C, Zhang X, Zheng F, Cai W, Vlassara H, et al. Advanced glycation end products inhibit glucose-stimulated insulin secretion through nitric oxide-dependent inhibition of cytochrome c oxidase and adenosine triphosphate synthesis. Endocrinology. 2009;150:2569-76 pubmed publisher..We conclude that AGEs inhibit cytochrome c oxidase and ATP production, leading to the impairment of glucose-stimulated insulin secretion through iNOS-dependent nitric oxide production. ..
- Hicks D, Liu J, Fujisawa M, Krulwich T. F1F0-ATP synthases of alkaliphilic bacteria: lessons from their adaptations. Biochim Biophys Acta. 2010;1797:1362-77 pubmed publisher..ATP synthesis in alkaliphiles depends upon alkaliphile-specific adaptations of the ATP synthase and there is also evidence for alkaliphile-specific adaptations of respiratory chain components. ..
- Emelyanov A, Vershilova E, Ignatyeva M, Pokrovsky D, Lu X, Konev A, et al. Identification and characterization of ToRC, a novel ISWI-containing ATP-dependent chromatin assembly complex. Genes Dev. 2012;26:603-14 pubmed publisher..Thus, Toutatis forms two alternative complexes that have differential distribution and can participate in distinct aspects of nuclear DNA metabolism. ..
- Scemes E, Suadicani S, Spray D. Intercellular communication in spinal cord astrocytes: fine tuning between gap junctions and P2 nucleotide receptors in calcium wave propagation. J Neurosci. 2000;20:1435-45 pubmed..Intercellular communication via calcium waves therefore is sustained in Cx43 null mice by a finely tuned interaction between gap junction-dependent and independent mechanisms. ..
- Martin D, Sachs J. Ligands presumed to label high affinity and low affinity ATP binding sites do not interact in an (alpha beta)2 diprotomer in duck nasal gland Na+,K+-ATPase, nor Do the sites coexist in native enzyme. J Biol Chem. 2000;275:24512-7 pubmed..Low affinity sites do not exist on the unmodified enzyme but can be detected only after the high affinity site is modified by FITC...
- Weber T, Parlati F, McNew J, Johnston R, Westermann B, Sollner T, et al. SNAREpins are functionally resistant to disruption by NSF and alphaSNAP. J Cell Biol. 2000;149:1063-72 pubmed..This elegant design allows fusion to proceed locally in the face of an overall environment that massively favors SNARE disruption. ..
- Habelhah H, Shah K, Huang L, Burlingame A, Shokat K, Ronai Z. Identification of new JNK substrate using ATP pocket mutant JNK and a corresponding ATP analogue. J Biol Chem. 2001;276:18090-5 pubmed..The newly identified substrate can be phosphorylated by JNK on amino acids 216 and 353, which contribute to heterogeneous nuclear ribonucleoprotein K mediated transcriptional activities. ..
- Morgan E, Shah N, Symington L. The requirement for ATP hydrolysis by Saccharomyces cerevisiae Rad51 is bypassed by mating-type heterozygosity or RAD54 in high copy. Mol Cell Biol. 2002;22:6336-43 pubmed
- Suadicani S, de Pina Benabou M, Urban Maldonado M, Spray D, Scemes E. Acute downregulation of Cx43 alters P2Y receptor expression levels in mouse spinal cord astrocytes. Glia. 2003;42:160-71 pubmed..On the basis of these results, we propose that Cx43 regulates both modes of ICW by altering P2Y receptor subtype expression in addition to providing intercellular coupling. ..
- Green A, Rumberger J, Stuart C, Ruhoff M. Stimulation of lipolysis by tumor necrosis factor-alpha in 3T3-L1 adipocytes is glucose dependent: implications for long-term regulation of lipolysis. Diabetes. 2004;53:74-81 pubmed..These findings suggest novel mechanisms by which TNF-alpha and hyperglycemia raise FFA levels and induce insulin resistance. ..
- Vergani P, Basso C, Mense M, Nairn A, Gadsby D. Control of the CFTR channel's gates. Biochem Soc Trans. 2005;33:1003-7 pubmed
- Malester B, Tong X, Ghiu I, Kontogeorgis A, Gutstein D, Xu J, et al. Transgenic expression of a dominant negative K(ATP) channel subunit in the mouse endothelium: effects on coronary flow and endothelin-1 secretion. FASEB J. 2007;21:2162-72 pubmed..These data are consistent with a role for endothelial K(ATP) channels to control the coronary blood flow by modulating the release of the vasoconstrictor, endothelin-1. ..
- Taldone T, Sun W, Chiosis G. Discovery and development of heat shock protein 90 inhibitors. Bioorg Med Chem. 2009;17:2225-35 pubmed publisher..This review will discuss the discovery of these different classes, as well as their development as potential clinical agents. ..
- Serban G, Whittaker V, Fan J, Liu Z, Manga K, Khan M, et al. Significance of immune cell function monitoring in renal transplantation after Thymoglobulin induction therapy. Hum Immunol. 2009;70:882-90 pubmed publisher..Although low ATP levels identify patients at increased risk for infection, high ATP values fail to correlate with rejection and do not justify increased immunosuppression. ..
- Zhang L, Gavin T, DeCaprio A, LoPachin R. Gamma-diketone axonopathy: analyses of cytoskeletal motors and highways in CNS myelinated axons. Toxicol Sci. 2010;117:180-9 pubmed publisher..Given their critical role in cytoskeletal physiology, MAPs could represent a relevant target for the induction of gamma-diketone axonopathy. ..
- Ivannikov M, Sugimori M, Llinas R. Synaptic vesicle exocytosis in hippocampal synaptosomes correlates directly with total mitochondrial volume. J Mol Neurosci. 2013;49:223-30 pubmed publisher..Thus, synaptic vesicle release is linked to the presynaptic ability to regenerate ATP, which itself is a utility of mitochondrial density and activity...
- Gorman J, Wang F, Redding S, Plys A, Fazio T, Wind S, et al. Single-molecule imaging reveals target-search mechanisms during DNA mismatch repair. Proc Natl Acad Sci U S A. 2012;109:E3074-83 pubmed publisher..Our findings highlight a hierarchy of lesion- and ATP-dependent transitions involving both MutS? and MutL?, and help establish how different modes of diffusion can be used during recognition and repair of damaged DNA. ..
- Hong M, Bao L, Kefaloyianni E, Agullo Pascual E, Chkourko H, Foster M, et al. Heterogeneity of ATP-sensitive K+ channels in cardiac myocytes: enrichment at the intercalated disk. J Biol Chem. 2012;287:41258-67 pubmed publisher..Our data demonstrate heterogeneity of K(ATP) channel distribution within a cardiac myocyte. The higher K(ATP) channel density at the intercalated disk implies a possible role at the intercellular junctions during cardiac ischemia. ..
- Vytla V, Ochs R. Metformin increases mitochondrial energy formation in L6 muscle cell cultures. J Biol Chem. 2013;288:20369-77 pubmed publisher..We conclude that, in intact cells, metformin can lead to a stimulation of energy formation, rather than an inhibition. ..
- Rangaraju V, Calloway N, Ryan T. Activity-driven local ATP synthesis is required for synaptic function. Cell. 2014;156:825-35 pubmed publisher..Despite this large reservoir of ATP, we find that several key aspects of presynaptic function are severely impaired following even brief interruptions in activity-stimulated ATP synthesis. ..
- Sorota S, Chlenov M, Du X, Kagan M. ATP-dependent activation of the atrial acetylcholine-induced K+ channel does not require nucleoside diphosphate kinase activity. Circ Res. 1998;82:971-9 pubmed..We hypothesize that the activation by millimolar concentrations of ATP is due to binding of adenine nucleotide to the guanine nucleotide binding site of the G protein(s) responsible for stimulating the acetylcholine-induced K+ current. ..
- Avivar Valderas A, Salas E, Bobrovnikova Marjon E, Diehl J, Nagi C, Debnath J, et al. PERK integrates autophagy and oxidative stress responses to promote survival during extracellular matrix detachment. Mol Cell Biol. 2011;31:3616-29 pubmed publisher..We propose that the normal proautophagic and antioxidant PERK functions may be hijacked to promote the survival of ECM-detached tumor cells in DCIS lesions. ..
- Urban C, Celis R. Purification and properties of a kinase from Escherichia coli K-12 that phosphorylates two periplasmic transport proteins. J Biol Chem. 1990;265:1783-6 pubmed..The ATPase activity of the enzyme yielded an apparent Km value for ATP of 50 microM. A similar value, Km of 100 microM, was calculated for the kinase activity with different concentrations of ATP. The enzyme showed a pH optimum of 7.3. ..
- Jeffrey P, Russo A, Polyak K, Gibbs E, Hurwitz J, Massague J, et al. Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex. Nature. 1995;376:313-20 pubmed..These changes activate the kinase by realigning active site residues and relieving the steric blockade at the entrance of the catalytic cleft. ..
- Leu F, O Donnell M. Interplay of clamp loader subunits in opening the beta sliding clamp of Escherichia coli DNA polymerase III holoenzyme. J Biol Chem. 2001;276:47185-94 pubmed..The implications of these actions for the workings of the E. coli clamp loading machinery and for eukaryotic RFC and PCNA are discussed. ..
- Ordonez H, Shuman S. Mycobacterium smegmatis Lhr Is a DNA-dependent ATPase and a 3'-to-5' DNA translocase and helicase that prefers to unwind 3'-tailed RNA:DNA hybrids. J Biol Chem. 2013;288:14125-34 pubmed publisher..Lhr homologs are found in bacteria representing eight different phyla, being especially prevalent in Actinobacteria (including M. tuberculosis) and Proteobacteria (including Escherichia coli). ..
- Nurse P, Bahng S, Mossessova E, Marians K. Mutational analysis of Escherichia coli topoisomerase IV. II. ATPase negative mutants of parE induce hyper-DNA cleavage. J Biol Chem. 2000;275:4104-11 pubmed..Thus, while ATP is not required for DNA cleavage, the properties of these mutant enzymes suggests that ATP-hydrolysis informs DNA religation. ..
- Berman M, Frazer A. Importance of tetrahydrofolate and ATP in the anaerobic O-demethylation reaction for phenylmethylethers. Appl Environ Microbiol. 1992;58:925-31 pubmed..woodii is oxygen sensitive. The THF- and ATP-dependent activity measurable in the soluble fraction of cell extracts constituted about 34% of the activity seen with intact cells. ..
- Cheng H, Isoda F, Belsham D, Mobbs C. Inhibition of agouti-related peptide expression by glucose in a clonal hypothalamic neuronal cell line is mediated by glycolysis, not oxidative phosphorylation. Endocrinology. 2008;149:703-10 pubmed..These studies support that cytoplasmic or nuclear NADH, uniquely produced by glucose metabolism, mediates effects of glucose on AgRP expression. ..
- Jankowsky E, Gross C, Shuman S, Pyle A. Active disruption of an RNA-protein interaction by a DExH/D RNA helicase. Science. 2001;291:121-5 pubmed..This indicates that DExH/D proteins can effectively catalyze protein displacement from RNA and thereby participate in the structural reorganization of ribonucleoprotein assemblies. ..
- Rasooly R, New C, Zhang P, Hawley R, Baker B. The lethal(1)TW-6cs mutation of Drosophila melanogaster is a dominant antimorphic allele of nod and is associated with a single base change in the putative ATP-binding domain. Genetics. 1991;129:409-22 pubmed..We propose that the defective protein encoded by the nodDTW allele interferes with proper chromosome movement during both meiosis and mitosis, perhaps by binding irreversibly to microtubules...
- Lima C, Klein M, Hendrickson W. Structure-based analysis of catalysis and substrate definition in the HIT protein family. Science. 1997;278:286-90 pubmed..The approach described here may be useful in identifying structure-function relations between protein families identified through genomics. ..
- Lin J, Lou N, Kang N, Takano T, Hu F, Han X, et al. A central role of connexin 43 in hypoxic preconditioning. J Neurosci. 2008;28:681-95 pubmed publisher..Thus, adaptive modulation of Cx43 can offset environmental stress by adenosine-mediated elevation of cellular resistance. ..
- Favelyukis S, Till J, Hubbard S, Miller W. Structure and autoregulation of the insulin-like growth factor 1 receptor kinase. Nat Struct Biol. 2001;8:1058-63 pubmed..Although the nucleotide binding cleft is conserved between IGF1RK and the insulin receptor kinase, sequence differences in the nearby interlobe linker could potentially be exploited for anticancer drug design. ..
- Varma H, Cheng R, Voisine C, Hart A, Stockwell B. Inhibitors of metabolism rescue cell death in Huntington's disease models. Proc Natl Acad Sci U S A. 2007;104:14525-30 pubmed..Our results reveal a mechanism for activating prosurvival signaling that could be exploited for treating HD and possibly other neurodegenerative disorders. ..
- Wang R, Liu C, Leyh T. Allosteric regulation of the ATP sulfurylase associated GTPase. Biochemistry. 1995;34:490-5 pubmed..These studies provide an in-depth view of the energetic interactions between the two active sites at each step of the APS-forming reaction. ..
- Lee K, Saiz J, Barton W, Fisher R. Cdc2 activation in fission yeast depends on Mcs6 and Csk1, two partially redundant Cdk-activating kinases (CAKs). Curr Biol. 1999;9:441-4 pubmed..Inactivation of both CAKs is necessary and sufficient to prevent Cdc2 activation and cause a cell-cycle arrest. Mcs6, which is essential, may therefore have required functions other than Cdk activation. ..
- Gatti D, Tzagoloff A. Structure and evolution of a group of related aminoacyl-tRNA synthetases. J Mol Biol. 1991;218:557-68 pubmed
- Iacobas D, Suadicani S, Spray D, Scemes E. A stochastic two-dimensional model of intercellular Ca2+ wave spread in glia. Biophys J. 2006;90:24-41 pubmed..Such modeling is expected to be useful for testing phenomenological hypotheses and in understanding consequences of alteration of system components under experimental or pathological conditions. ..
- Marina A, Waldburger C, Hendrickson W. Structure of the entire cytoplasmic portion of a sensor histidine-kinase protein. EMBO J. 2005;24:4247-59 pubmed publisher..Mutational tests support the functional relevance of interdomain contacts...
- Pocai A, Lam T, Gutierrez Juarez R, Obici S, Schwartz G, Bryan J, et al. Hypothalamic K(ATP) channels control hepatic glucose production. Nature. 2005;434:1026-31 pubmed..These findings suggest that activation of hypothalamic K(ATP) channels normally restrains hepatic gluconeogenesis, and that any alteration within this central nervous system/liver circuit can contribute to diabetic hyperglycaemia...
- Poulikakos P, Zhang C, Bollag G, Shokat K, Rosen N. RAF inhibitors transactivate RAF dimers and ERK signalling in cells with wild-type BRAF. Nature. 2010;464:427-30 pubmed publisher..In agreement with this prediction, RAF inhibitors do not inhibit ERK signalling in cells that coexpress BRAF(V600E) and mutant RAS. ..
- Dworkin M, Dworkin Rastl E. Metabolic regulation during early frog development. Identification of proteins labeled by 32P-glycolytic intermediates. J Biol Chem. 1987;262:17038-45 pubmed..This last result is expected in tissues showing metabolic flux largely in the glycolytic direction. The data indicate that in full-grown oocytes and embryos metabolic flux occurs largely in the gluconeogenic direction. ..
- Morrissey A, Parachuru L, Leung M, Lopez G, Nakamura T, Tong X, et al. Expression of ATP-sensitive K+ channel subunits during perinatal maturation in the mouse heart. Pediatr Res. 2005;58:185-92 pubmed..Our data suggest that the K(ATP) channel composition may change during maturation, which has important implications for K(ATP) channel function in the developing heart...
- Yuan Y, Blecker S, Martsinkevich O, Millen L, Thomas P, Hunt J. The crystal structure of the MJ0796 ATP-binding cassette. Implications for the structural consequences of ATP hydrolysis in the active site of an ABC transporter. J Biol Chem. 2001;276:32313-21 pubmed
- Epshtein V, Nudler E. Cooperation between RNA polymerase molecules in transcription elongation. Science. 2003;300:801-5 pubmed..Such cooperation between RNAP molecules links the rate of elongation to the rate of initiation and explains why elongation is still fast and processive in vivo even without anti-arrest factors. ..
- Seiffert K, Ding W, Wagner J, Granstein R. ATPgammaS enhances the production of inflammatory mediators by a human dermal endothelial cell line via purinergic receptor signaling. J Invest Dermatol. 2006;126:1017-27 pubmed..For example, ATP may contribute to both the vasodilation and the inflammation associated with rosacea. ..
- Machida T, Heerdt P, Reid A, Schäfer U, Silver R, Broekman M, et al. Ectonucleoside triphosphate diphosphohydrolase 1/CD39, localized in neurons of human and porcine heart, modulates ATP-induced norepinephrine exocytosis. J Pharmacol Exp Ther. 2005;313:570-7 pubmed..By curtailing NE release, in addition to its effects as an antithrombotic agent, soluble CD39 may constitute a novel therapeutic approach to ischemic complications in the myocardium. ..
- Thanassi D, Stathopoulos C, Karkal A, Li H. Protein secretion in the absence of ATP: the autotransporter, two-partner secretion and chaperone/usher pathways of gram-negative bacteria (review). Mol Membr Biol. 2005;22:63-72 pubmed..This review will present overviews of these 'self-sufficient' pathways, focusing on recent advances and secretion mechanisms. Similarities among the pathways and with other protein translocation mechanisms will be highlighted. ..
- Dousmanis A, Nairn A, Gadsby D. Distinct Mg(2+)-dependent steps rate limit opening and closing of a single CFTR Cl(-) channel. J Gen Physiol. 2002;119:545-59 pubmed..Such stabilization of the open-channel conformation of CFTR by tight binding, or occlusion, of an ATP molecule echoes the stabilization of the active conformation of a G protein by GTP. ..
- Pei Y, Shuman S. Characterization of the Schizosaccharomyces pombe Cdk9/Pch1 protein kinase: Spt5 phosphorylation, autophosphorylation, and mutational analysis. J Biol Chem. 2003;278:43346-56 pubmed
- Magnet S, Blanchard J. Mechanistic and kinetic study of the ATP-dependent DNA ligase of Neisseria meningitidis. Biochemistry. 2004;43:710-7 pubmed publisher..008 s(-1), is the result of three chemical steps with similar rate constants (approximately 0.025 s(-1)). The Km values for ATP and DNA substrates, in the overall ligation reaction, were 0.4 microM and 30 nM, respectively...
- Bao L, Avshalumov M, Rice M. Partial mitochondrial inhibition causes striatal dopamine release suppression and medium spiny neuron depolarization via H2O2 elevation, not ATP depletion. J Neurosci. 2005;25:10029-40 pubmed..Overall, these novel factors could contribute to parkinsonian motor deficits and neuronal degeneration caused by mitochondrial dysfunction. ..
- Shen J, Snapp E, Lippincott Schwartz J, Prywes R. Stable binding of ATF6 to BiP in the endoplasmic reticulum stress response. Mol Cell Biol. 2005;25:921-32 pubmed..We propose that stable BiP binding is essential for ATF6 regulation and that ER stress dissociates BiP from ATF6 by actively restarting the BiP ATPase cycle. ..
- Ellison V, Stillman B. Biochemical characterization of DNA damage checkpoint complexes: clamp loader and clamp complexes with specificity for 5' recessed DNA. PLoS Biol. 2003;1:E33 pubmed
- Kang J, Kang N, Lovatt D, Torres A, Zhao Z, Lin J, et al. Connexin 43 hemichannels are permeable to ATP. J Neurosci. 2008;28:4702-11 pubmed publisher..Furthermore, a putative Cx43 hemichannel with characteristics identical to the Cx43 hemichannel in C6 cells was identified in the membrane of hippocampal astrocytes in acutely prepared slices. ..
- Thelen M, Rosen A, Nairn A, Aderem A. Regulation by phosphorylation of reversible association of a myristoylated protein kinase C substrate with the plasma membrane. Nature. 1991;351:320-2 pubmed..8, and J. Hartwig et al., manuscript submitted), the cycle of membrane attachment/detachment represents a mechanism through which PKC might reversibly regulate actin-membrane interaction. ..
- Kimura K, Rybenkov V, Crisona N, Hirano T, Cozzarelli N. 13S condensin actively reconfigures DNA by introducing global positive writhe: implications for chromosome condensation. Cell. 1999;98:239-48 pubmed..Condensin bound preferentially to (+) supercoiled DNA in the presence of ATP but not in its absence. Our results suggest a mechanism for the compaction of chromatin by condensin during mitosis. ..
- Manfredi G, Fu J, Ojaimi J, Sadlock J, Kwong J, Guy J, et al. Rescue of a deficiency in ATP synthesis by transfer of MTATP6, a mitochondrial DNA-encoded gene, to the nucleus. Nat Genet. 2002;30:394-9 pubmed..This is the first successful demonstration of allotopic expression of an mtDNA-encoded polypeptide in mammalian cells and could form the basis of a genetic approach to treat a number of human mitochondrial disorders. ..
- Caplan A, Mandal A, Theodoraki M. Molecular chaperones and protein kinase quality control. Trends Cell Biol. 2007;17:87-92 pubmed..This requirement might relate to conformational changes that take place during the protein kinase activity cycle. ..
- Santorelli F, Mak S, Vazquez Memije M, Shanske S, Kranz Eble P, Jain K, et al. Clinical heterogeneity associated with the mitochondrial DNA T8993C point mutation. Pediatr Res. 1996;39:914-7 pubmed..These findings suggest that the T8993C mutation is less severe than the more common T8993G mutation. ..
- Frydman J, Hartl F. Principles of chaperone-assisted protein folding: differences between in vitro and in vivo mechanisms. Science. 1996;272:1497-502 pubmed..Thus, folding, oligomerization, and degradation are linked hierarchically to ensure the correct fate of newly synthesized polypeptides. ..
- Schaefer U, Machida T, Broekman M, Marcus A, Levi R. Targeted deletion of ectonucleoside triphosphate diphosphohydrolase 1/CD39 leads to desensitization of pre- and postsynaptic purinergic P2 receptors. J Pharmacol Exp Ther. 2007;322:1269-77 pubmed..Moreover, by virtue of its antithrombotic action CD39 can potentially prevent the transition from myocardial ischemia to infarction. ..
- Liu C, Martin E, Leyh T. GTPase activation of ATP sulfurylase: the mechanism. Biochemistry. 1994;33:2042-7 pubmed..Product inhibition studies indicate that PPi release occurs prior to the addition of sulfate and APS formation. These results are used to construct a proposed mechanism for the GTP-activated synthesis of APS. ..
- Obici S, Feng Z, Morgan K, Stein D, Karkanias G, Rossetti L. Central administration of oleic acid inhibits glucose production and food intake. Diabetes. 2002;51:271-5 pubmed..This is the first demonstration that fatty acids can signal nutrient availability to the CNS, which in turn limits further delivery of nutrients to the circulation. ..
- Prasad T, Yeykal C, Greene E. Visualizing the assembly of human Rad51 filaments on double-stranded DNA. J Mol Biol. 2006;363:713-28 pubmed..The results presented here also provide an important initial framework for beginning to study the biochemical behaviors of Rad51 nucleoprotein filaments using our novel experimental system. ..
- Acin Perez R, Gatti D, Bai Y, Manfredi G. Protein phosphorylation and prevention of cytochrome oxidase inhibition by ATP: coupled mechanisms of energy metabolism regulation. Cell Metab. 2011;13:712-9 pubmed publisher..We propose that S58 COXIV-1 phosphorylation has evolved as a metabolic switch that allows mammalian mitochondria to rapidly toggle between energy utilization and energy storage. ..
- Kaplan D, O Donnell M. DnaB drives DNA branch migration and dislodges proteins while encircling two DNA strands. Mol Cell. 2002;10:647-57 pubmed..DnaB binds to just one DNA strand during branch migration. T7 phage gp4 protein also drives DNA branch migration, suggesting this activity generalizes to other ring-shaped helicases. ..
- Rybin V, Guo J, Steinberg S. Protein kinase D1 autophosphorylation via distinct mechanisms at Ser744/Ser748 and Ser916. J Biol Chem. 2009;284:2332-43 pubmed publisher..Rather, autophosphorylation at Ser916 is required for subsequent autophosphorylation at Ser748. Finally, this study exposes a novel role for Ser916 and/or Ser748 autophosphorylation to terminate the cellular PKD1 signaling response. ..