Experts and Doctors on escherichia coli in Nottingham, England, United Kingdom

Summary

Locale: Nottingham, England, United Kingdom
Topic: escherichia coli

Top Publications

  1. Old I, Hunter M, Wilson D, Knight S, Weatherston C, Glass R. Cloning and characterization of the genes for the two homocysteine transmethylases of Escherichia coli. Mol Gen Genet. 1988;211:78-87 pubmed
    ..Under conditions whereby both forms of negative metE control are non-functional, the metE gene product represented about 90% of the total protein, and cell growth was severely impaired. ..
  2. Majsec K, Bolt E, Ivančić Baće I. Cas3 is a limiting factor for CRISPR-Cas immunity in Escherichia coli cells lacking H-NS. BMC Microbiol. 2016;16:28 pubmed publisher
    ..Regulation of cas3 is responsive to phase of growth, and to growth temperature in E. coli, impacting on the efficacy of CRISPR-Cas immunity in these experimental systems. ..
  3. Upton A, Grove J, Mahdi A, Briggs G, Milner D, Rudolph C, et al. Cellular location and activity of Escherichia coli RecG proteins shed light on the function of its structurally unresolved C-terminus. Nucleic Acids Res. 2014;42:5702-14 pubmed publisher
    ..More significantly, the extreme C-terminal mutations have little effect on localisation. Mutations that do prevent localisation result in only a slight reduction in the capacity for DNA repair. ..
  4. Mahdi A, Briggs G, Lloyd R. Modulation of DNA damage tolerance in Escherichia coli recG and ruv strains by mutations affecting PriB, the ribosome and RNA polymerase. Mol Microbiol. 2012;86:675-91 pubmed publisher
  5. Stockum A, Lloyd R, Rudolph C. On the viability of Escherichia coli cells lacking DNA topoisomerase I. BMC Microbiol. 2012;12:26 pubmed publisher
    ..Potential reasons for this discrepancy are discussed. ..
  6. Lerner T, Lovering A, Bui N, Uchida K, Aizawa S, Vollmer W, et al. Specialized peptidoglycan hydrolases sculpt the intra-bacterial niche of predatory Bdellovibrio and increase population fitness. PLoS Pathog. 2012;8:e1002524 pubmed publisher
  7. Atterbury R, Hobley L, Till R, Lambert C, Capeness M, Lerner T, et al. Effects of orally administered Bdellovibrio bacteriovorus on the well-being and Salmonella colonization of young chicks. Appl Environ Microbiol. 2011;77:5794-803 pubmed publisher
    ..This work is a first step to applying Bdellovibrio therapeutically for other animal, and possibly human, infections...
  8. Rudolph C, Upton A, Lloyd R. Maintaining replication fork integrity in UV-irradiated Escherichia coli cells. DNA Repair (Amst). 2008;7:1589-602 pubmed publisher
    ..Instead, nascent strands may be protected by the simple expedient of promoting restart. We also report that the efficiency of fork reactivation is not affected in polB mutants. ..
  9. Zou C, Thomas M, Keen J, Glass R. A nested set of C-terminal deletions of the alpha subunit of Escherichia coli RNA polymerase define regions concerned with assembly, proteolysis, stabilization and transcriptional activation in vivo. Genes Cells. 1997;2:81-94 pubmed
    ..Their presence is also essential for transcription activation at class I CRP-dependent promoters. The rpoA341-mediated substitution, K271E, does not define an activation contact site for MelR. ..

More Information

Publications19

  1. Tavender T, Halliday N, Hardie K, Winzer K. LuxS-independent formation of AI-2 from ribulose-5-phosphate. BMC Microbiol. 2008;8:98 pubmed publisher
    ..It does not contribute to AI-2 formation in wildtype E. coli and S. aureus under the conditions tested, but may be responsible for the AI-2-like activities reported for other organisms lacking the luxS gene. ..
  2. Rudolph C, Upton A, Harris L, Lloyd R. Pathological replication in cells lacking RecG DNA translocase. Mol Microbiol. 2009;73:352-66 pubmed publisher
    ..Our results shed light on why replication initiation in bacteria is limited to a single origin and why termination is carefully orchestrated to a single event within a restricted area each cell cycle...
  3. Rudolph C, Upton A, Lloyd R. Replication fork collisions cause pathological chromosomal amplification in cells lacking RecG DNA translocase. Mol Microbiol. 2009;74:940-55 pubmed publisher
  4. Wand M, Sockett R, Evans K, Doherty N, Sharp P, Hardie K, et al. Helicobacter pylori FlhB function: the FlhB C-terminal homologue HP1575 acts as a "spare part" to permit flagellar export when the HP0770 FlhBCC domain is deleted. J Bacteriol. 2006;188:7531-41 pubmed publisher
    ..However, mutation of the proline contained within the NPTH site of HP0770 did not affect motility and flagellar assembly, although it clearly interfered with processing when the protein was heterologously produced in Escherichia coli...
  5. Clark G, Paszkiewicz K, Hale J, Weston V, Constantinidou C, Penn C, et al. Genomic analysis uncovers a phenotypically diverse but genetically homogeneous Escherichia coli ST131 clone circulating in unrelated urinary tract infections. J Antimicrob Chemother. 2012;67:868-77 pubmed publisher
    ..To determine variation at the genome level in Escherichia coli ST131 clinical isolates previously shown to be phenotypically diverse...
  6. McGlynn P, Lloyd R. Genome stability and the processing of damaged replication forks by RecG. Trends Genet. 2002;18:413-9 pubmed
    ..Such direct rescue of stalled forks might help safeguard genome integrity in all organisms. ..
  7. Bolt E, Lloyd R, Sharples G. Genetic analysis of an archaeal Holliday junction resolvase in Escherichia coli. J Mol Biol. 2001;310:577-89 pubmed
    ..These results support models in which RecG acts at a replication fork stalled at a lesion in the DNA, catalysing fork regression and forming a Holliday junction that can then be acted upon by Hjc. ..
  8. Layfield R, Franklin K, Landon M, Walker G, Wang P, Ramage R, et al. Chemically synthesized ubiquitin extension proteins detect distinct catalytic capacities of deubiquitinating enzymes. Anal Biochem. 1999;274:40-9 pubmed
    ..This work demonstrates that the synthetic approach offers an alternative to recombinant methods for the production of small proteins in vitro. ..
  9. Capeness M, Lambert C, Lovering A, Till R, Uchida K, Chaudhuri R, et al. Activity of Bdellovibrio hit locus proteins, Bd0108 and Bd0109, links Type IVa pilus extrusion/retraction status to prey-independent growth signalling. PLoS ONE. 2013;8:e79759 pubmed publisher
    ..We further identify a novel small RNA encoded by the hit locus, the transcription of which is altered in different bd0108 mutation backgrounds. ..
  10. Holland J, Towner K, Williams P. Tn916 insertion mutagenesis in Escherichia coli and Haemophilus influenzae type b following conjugative transfer. J Gen Microbiol. 1992;138:509-15 pubmed
    ..Tn916 insertion mutagenesis, following transfer by conjugation, is a convenient method for isolating mutations in genes concerned with iron acquisition by this important human pathogen. ..