Experts and Doctors on electron spin resonance spectroscopy in Norwich, England, United Kingdom


Locale: Norwich, England, United Kingdom
Topic: electron spin resonance spectroscopy

Top Publications

  1. Cheesman M, Thomson A, Greenwood C, Moore G, Kadir F. Bis-methionine axial ligation of haem in bacterioferritin from Pseudomonas aeruginosa. Nature. 1990;346:771-3 pubmed
    ..aeruginosa bacterioferritin which enable the axial ligands to be identified as the thioether side chains of two methionine residues, a ligation scheme not previously reported for haem in any protein. ..
  2. Bennett B, Gruer M, Guest J, Thomson A. Spectroscopic characterisation of an aconitase (AcnA) of Escherichia coli. Eur J Biochem. 1995;233:317-26 pubmed
    ..A novel S = 1/2 EPR signal with g approximately 2 was observed in AcnA upon treatment with EDTA. The species giving rise to this signal is proposed to be an intermediate in cluster deconstruction...
  3. Le Brun N, Wilson M, Andrews S, Guest J, Harrison P, Thomson A, et al. Kinetic and structural characterization of an intermediate in the biomineralization of bacterioferritin. FEBS Lett. 1993;333:197-202 pubmed
  4. Le Brun N, Cheesman M, Thomson A, Moore G, Andrews S, Guest J, et al. An EPR investigation of non-haem iron sites in Escherichia coli bacterioferritin and their interaction with phosphate. A study using nitric oxide as a spin probe. FEBS Lett. 1993;323:261-6 pubmed
    ..Under certain conditions, an iron dimer was also observed. The reaction of phosphate with NHI species has been investigated. Results point to a function for this anion in core nucleation. ..
  5. Cheesman M, Le Brun N, Kadir F, Thomson A, Moore G, Andrews S, et al. Haem and non-haem iron sites in Escherichia coli bacterioferritin: spectroscopic and model building studies. Biochem J. 1993;292 ( Pt 1):47-56 pubmed
    ..293, 164-168] and the possibility that this Fe(III) ion may reside at or near the ferroxidase centre is discussed. ..
  6. Keech A, Le Brun N, Wilson M, Andrews S, Moore G, Thomson A. Spectroscopic studies of cobalt(II) binding to Escherichia coli bacterioferritin. J Biol Chem. 1997;272:422-9 pubmed
    ..Discrimination against oxidation of single iron(II) ions avoids odd electron reduction products of oxygen. ..
  7. Thomson A, Le Brun N, Keech A, Andrews S, Moore G. Pumping iron: does bacterioferritin contain a redox-driven iron pump?. Biochem Soc Trans. 1997;25:96-101 pubmed
  8. Le Brun N, Andrews S, Moore G, Thomson A. Interaction of nitric oxide with non-haem iron sites of Escherichia coli bacterioferritin: reduction of nitric oxide to nitrous oxide and oxidation of iron(II) to iron(III). Biochem J. 1997;326 ( Pt 1):173-9 pubmed
    ..This is consistent with a proposal that the diferric form of the centre is unstable and breaks down to form mononuclear iron species. ..
  9. Bamford V, Angove H, Seward H, Thomson A, Cole J, Butt J, et al. Structure and spectroscopy of the periplasmic cytochrome c nitrite reductase from Escherichia coli. Biochemistry. 2002;41:2921-31 pubmed
    ..Previous spectroscopic studies on NrfA from a number of bacterial species are considered in the light of the structure-based spectro-potentiometric analysis presented for the E. coli NrfA...

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  1. Harris R, Eady R, Hasnain S, Sawers R. Coordinate synthesis of azurin I and copper nitrite reductase in Alcaligenes xylosoxidans during denitrification. Arch Microbiol. 2006;186:241-9 pubmed
    ..Taken together, these findings indicate that in A. xylosoxidans azuA expression is coordinated with denitrification and recombinant Az I is processed and matured in the periplasm of E. coli in the same way it is in A. xylosoxidans. ..
  2. White G, Field S, Marritt S, Oganesyan V, Gennis R, Yap L, et al. An EPR spin label study of the quinol oxidase, E. coli cytochrome bo3: a search for redox induced conformational changes. Biochemistry. 2007;46:2355-63 pubmed
    ..Because kinetic studies of mutants show that the redox gating of protons occurs deep within the D channel close to the heme-copper site, the present study implies that no motion is transmitted to the ends of the helices. ..
  3. Field S, Thorndycroft F, Matorin A, Richardson D, Watmough N. The respiratory nitric oxide reductase (NorBC) from Paracoccus denitrificans. Methods Enzymol. 2008;437:79-101 pubmed publisher
  4. Tucker N, Hicks M, Clarke T, Crack J, Chandra G, Le Brun N, et al. The transcriptional repressor protein NsrR senses nitric oxide directly via a [2Fe-2S] cluster. PLoS ONE. 2008;3:e3623 pubmed publisher
    ..Removal of the [2Fe-2S] cluster to generate apo-NsrR also resulted in loss of DNA binding activity. This is the first demonstration that NsrR contains an NO-sensitive [2Fe-2S] cluster that is required for DNA binding activity. ..