Genomes and Genes
Experts and Doctors on caenorhabditis elegans in Boulder, Colorado, United States
Locale: Boulder, Colorado, United States
Topic: caenorhabditis elegans
- Kniazeva M, Euler T, Han M. A branched-chain fatty acid is involved in post-embryonic growth control in parallel to the insulin receptor pathway and its biosynthesis is feedback-regulated in C. elegans. Genes Dev. 2008;22:2102-10 pubmed publisher..Our data suggest that C17ISO may act as a chemical/nutritional factor in a mechanism that regulates post-embryonic development in response to the metabolic state of the organism. ..
- Wu D, Cypser J, Yashin A, Johnson T. Multiple mild heat-shocks decrease the Gompertz component of mortality in Caenorhabditis elegans. Exp Gerontol. 2009;44:607-12 pubmed publisher..Thus, multiple heat-shocks have anti-aging hormetic effects and represent an effective approach for modulating aging. ..
- Parrish J, Li L, Klotz K, Ledwich D, Wang X, Xue D. Mitochondrial endonuclease G is important for apoptosis in C. elegans. Nature. 2001;412:90-4 pubmed..CPS-6 is the first mitochondrial protein identified to be involved in programmed cell death in C. elegans, underscoring the conserved and important role of mitochondria in the execution of apoptosis. ..
- Zhang X, Zabinsky R, Teng Y, Cui M, Han M. microRNAs play critical roles in the survival and recovery of Caenorhabditis elegans from starvation-induced L1 diapause. Proc Natl Acad Sci U S A. 2011;108:17997-8002 pubmed publisher..The presented results indicate that interactions between multiple miRNAs and likely a large number of their mRNA targets in multiple pathways regulate the response to starvation-induced L1 diapause. ..
- Morita K, Hirono K, Han M. The Caenorhabditis elegans ect-2 RhoGEF gene regulates cytokinesis and migration of epidermal P cells. EMBO Rep. 2005;6:1163-8 pubmed..We propose a model in which ECT-2GEF not only activates RHO-1 for P-cell cytokinesis, but also collaborates with UNC-73GEF and at least two Rac proteins to regulate P-cell migration. ..
- Mapes J, Chen J, Yu J, Xue D. Somatic sex determination in Caenorhabditis elegans is modulated by SUP-26 repression of tra-2 translation. Proc Natl Acad Sci U S A. 2010;107:18022-7 pubmed publisher..Taken together, our results provide further insight into how mRNA-binding factors repress translation and modulate sexual development in different tissues of C. elegans. ..
- Robida M, Singh R. Drosophila polypyrimidine-tract binding protein (PTB) functions specifically in the male germline. EMBO J. 2003;22:2924-33 pubmed..This male-specific expression of PTB is conserved in D.virilis. Thus, PTB appears to be a particularly potent downstream target of the sex-determination pathway in the male germline, since it can regulate multiple mRNAs. ..
- Antoshechkin I, Han M. The C. elegans evl-20 gene is a homolog of the small GTPase ARL2 and regulates cytoskeleton dynamics during cytokinesis and morphogenesis. Dev Cell. 2002;2:579-91 pubmed..Our data indicate that EVL-20 functions in the cytoplasm and at the plasma membrane to regulate cytoskeletal dynamics during cytokinesis and morphogenesis. ..
- Sieburth D, Sundaram M, Howard R, Han M. A PP2A regulatory subunit positively regulates Ras-mediated signaling during Caenorhabditis elegans vulval induction. Genes Dev. 1999;13:2562-9 pubmed..Double mutant analysis suggests that sur-6 PP2A-B acts downstream or in parallel to ras, but likely upstream of raf, and functions with ksr-1 in a common pathway to positively regulate Ras signaling. ..
- Wu D, Rea S, Cypser J, Johnson T. Mortality shifts in Caenorhabditis elegans: remembrance of conditions past. Aging Cell. 2009;8:666-75 pubmed publisher..However, 'b' (the rate of mortality increase with age) is always specified by the current conditions. ..
- Suzuki Y, Yandell M, Roy P, Krishna S, Savage Dunn C, Ross R, et al. A BMP homolog acts as a dose-dependent regulator of body size and male tail patterning in Caenorhabditis elegans. Development. 1999;126:241-50 pubmed..Our study of the dbl-1 expression pattern suggests a role for neuronal cells in global size regulation as well as male tail patterning. ..
- Cui M, Allen M, Larsen A, MacMorris M, Han M, Blumenthal T. Genes involved in pre-mRNA 3'-end formation and transcription termination revealed by a lin-15 operon Muv suppressor screen. Proc Natl Acad Sci U S A. 2008;105:16665-70 pubmed publisher..Furthermore, our results implicate a serine/arginine-rich (SR) protein, SRp20, in events following 3'-end cleavage, leading to termination of transcription. ..
- Link C, Taft A, Kapulkin V, Duke K, Kim S, Fei Q, et al. Gene expression analysis in a transgenic Caenorhabditis elegans Alzheimer's disease model. Neurobiol Aging. 2003;24:397-413 pubmed..elegans model. Both CRYAB and TNFAIP1 show increased transcript levels in AD brains, supporting the validity of this approach. ..
- Suzuki Y, Morris G, Han M, Wood W. A cuticle collagen encoded by the lon-3 gene may be a target of TGF-beta signaling in determining Caenorhabditis elegans body shape. Genetics. 2002;162:1631-9 pubmed..These results support the possibility that TGF-beta signaling controls C. elegans body shape by regulating cuticle composition...
- Powell Coffman J, Knight J, Wood W. Onset of C. elegans gastrulation is blocked by inhibition of embryonic transcription with an RNA polymerase antisense RNA. Dev Biol. 1996;178:472-83 pubmed..These results indicate that embryonically transcribed gene products are required for gastrulation initiation. They also demonstrate the efficacy of a method for blocking embryonic transcription that may be useful in other organisms. ..
- McIntosh J, O TOOLE E, Zhudenkov K, Morphew M, Schwartz C, Ataullakhanov F, et al. Conserved and divergent features of kinetochores and spindle microtubule ends from five species. J Cell Biol. 2013;200:459-74 pubmed publisher..Flaring protofilaments linked to chromatin are well placed to exert force on chromosomes, assuring stable attachment and reliable anaphase segregation. ..
- Nichols A, Meelkop E, Linton C, Giordano Santini R, Sullivan R, Donato A, et al. The Apoptotic Engulfment Machinery Regulates Axonal Degeneration in C.Â elegans Neurons. Cell Rep. 2016;14:1673-1683 pubmed publisher..Thus, our results reveal the existence of a WLD(S)/Nmnat-independent axonal degeneration pathway, conservation of the axonal clearance machinery, and a function for CED-7 and NRF-5 in this process. ..
- Kniazeva M, Sieber M, McCauley S, Zhang K, Watts J, Han M. Suppression of the ELO-2 FA elongation activity results in alterations of the fatty acid composition and multiple physiological defects, including abnormal ultradian rhythms, in Caenorhabditis elegans. Genetics. 2003;163:159-69 pubmed..elegans. The presented data indicate that suppression of a single enzyme of the FA elongation machinery is enough to affect various organs and systems in worms. This effect resembles syndromic disorders in humans. ..
- Hassan W, Merin D, Fonte V, Link C. AIP-1 ameliorates beta-amyloid peptide toxicity in a Caenorhabditis elegans Alzheimer's disease model. Hum Mol Genet. 2009;18:2739-47 pubmed publisher..Our results implicate AIP-1 in the regulation of protein turnover and protection against Abeta toxicity and point at AIRAPL as the functional mammalian homologue of AIP-1. ..
- Yochem J, Tuck S, Greenwald I, Han M. A gp330/megalin-related protein is required in the major epidermis of Caenorhabditis elegans for completion of molting. Development. 1999;126:597-606 pubmed..These observations indicate that LRP-1 is related to megalin not only structurally but also functionally. ..
- Wang X, Wang J, Gengyo Ando K, Gu L, Sun C, Yang C, et al. C. elegans mitochondrial factor WAH-1 promotes phosphatidylserine externalization in apoptotic cells through phospholipid scramblase SCRM-1. Nat Cell Biol. 2007;9:541-9 pubmed..Thus WAH-1, after its release from mitochondria during apoptosis, promotes plasma membrane phosphatidylserine externalization through its downstream effector, SCRM-1. ..
- Mapes J, Chen Y, Kim A, Mitani S, Kang B, Xue D. CED-1, CED-7, and TTR-52 regulate surface phosphatidylserine expression on apoptotic and phagocytic cells. Curr Biol. 2012;22:1267-75 pubmed publisher..In addition, some living cells such as macrophages also express exPS...
- Tucker M, Sieber M, Morphew M, Han M. The Caenorhabditis elegans aristaless orthologue, alr-1, is required for maintaining the functional and structural integrity of the amphid sensory organs. Mol Biol Cell. 2005;16:4695-704 pubmed..Genetic interaction tests also suggest that ALR-1 may function cooperatively with the cell adhesion processes in maintaining the amphid sensory organs...
- Spencer A, Orita S, Malone C, Han M. A RHO GTPase-mediated pathway is required during P cell migration in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2001;98:13132-7 pubmed..Finally, we provide evidence to support the idea that other small Rac subfamily small GTPases act redundantly and in parallel to RHO-1 in this specific cell migration event. ..
- Kell A, Ventura N, Kahn N, Johnson T. Activation of SKN-1 by novel kinases in Caenorhabditis elegans. Free Radic Biol Med. 2007;43:1560-6 pubmed..Inhibition of two of these kinases results in shorter life span and increased sensitivity to stress. ..
- Breckenridge D, Kang B, Xue D. Bcl-2 proteins EGL-1 and CED-9 do not regulate mitochondrial fission or fusion in Caenorhabditis elegans. Curr Biol. 2009;19:768-73 pubmed publisher..elegans. Taken together, our results argue against an evolutionarily conserved role for Bcl-2 proteins in regulating mitochondrial fission and fusion. ..
- Darland Ransom M, Wang X, Sun C, Mapes J, Gengyo Ando K, Mitani S, et al. Role of C. elegans TAT-1 protein in maintaining plasma membrane phosphatidylserine asymmetry. Science. 2008;320:528-31 pubmed publisher..Thus, tat-1 appears to function in preventing appearance of PS in the outer leaflet of plasma membrane, and ectopic exposure of PS on the cell surface may result in removal of living cells by neighboring phagocytes. ..
- Wang X, Li W, Zhao D, Liu B, Shi Y, Chen B, et al. Caenorhabditis elegans transthyretin-like protein TTR-52 mediates recognition of apoptotic cells by the CED-1 phagocyte receptor. Nat Cell Biol. 2010;12:655-64 pubmed publisher..TTR-52 is therefore the first bridging molecule identified in C. elegans that mediates recognition of apoptotic cells by crosslinking the PtdSer 'eat me' signal with the phagocyte receptor CED-1. ..
- Chen Z, Han M. Role of C. elegans lin-40 MTA in vulval fate specification and morphogenesis. Development. 2001;128:4911-21 pubmed..This inhibitory function of lin-40 might be carried out by downregulating lin-39 Hox expression. We also show that lin-40 is specifically required for cell divisions along the transverse orientation during vulval morphogenesis. ..
- Suzuki Y, Han M. Genetic redundancy masks diverse functions of the tumor suppressor gene PTEN during C. elegans development. Genes Dev. 2006;20:423-8 pubmed..We provide evidence that daf-18 elicits some functions independent of the downstream gene daf-16...
- Wu D, Cypser J, Yashin A, Johnson T. The U-shaped response of initial mortality in Caenorhabditis elegans to mild heat shock: does it explain recent trends in human mortality?. J Gerontol A Biol Sci Med Sci. 2008;63:660-8 pubmed..The dose of heat shock that coincided with this benefit in initial mortality did not affect the rate of increase in mortality. ..
- Yu H, Lai H, Lin T, Lo S. Autonomous and non-autonomous roles of DNaseÂ II during cell death in C. elegans embryos. Biosci Rep. 2015;35: pubmed publisher..Collectively, we demonstrate that the ToLFP method can be used to differentiate the locations of blastomeres where DNase II acts autonomously or non-autonomously in degrading apoptotic DNA. ..
- Parrish J, Xue D. Functional genomic analysis of apoptotic DNA degradation in C. elegans. Mol Cell. 2003;11:987-96 pubmed..It should now be possible to systematically decipher the mechanisms of apoptotic DNA degradation. ..
- Van Auken K, Weaver D, Robertson B, Sundaram M, Saldi T, Edgar L, et al. Roles of the Homothorax/Meis/Prep homolog UNC-62 and the Exd/Pbx homologs CEH-20 and CEH-40 in C. elegans embryogenesis. Development. 2002;129:5255-68 pubmed
- Johnson T. 25 years after age-1: genes, interventions and the revolution in aging research. Exp Gerontol. 2013;48:640-3 pubmed publisher..This area has been fascinating to those studying the sociology of science as modern aging research has moved to replace the simplistic, poorly controlled and outright fictitious approaches seen in much of the previous aging research. ..
- Geng X, Shi Y, Nakagawa A, Yoshina S, Mitani S, Shi Y, et al. Inhibition of CED-3 zymogen activation and apoptosis in Caenorhabditis elegans by caspase homolog CSP-3. Nat Struct Mol Biol. 2008;15:1094-101 pubmed publisher..However, CSP-3 does not block CED-3 activation induced by CED-4, nor does it inhibit the activity of the activated CED-3 protease. Therefore CSP-3 uses a previously unreported mechanism to protect cells from apoptosis. ..
- Zhang L, Ding L, Cheung T, Dong M, Chen J, Sewell A, et al. Systematic identification of C. elegans miRISC proteins, miRNAs, and mRNA targets by their interactions with GW182 proteins AIN-1 and AIN-2. Mol Cell. 2007;28:598-613 pubmed..Our results demonstrate an effective biochemical approach to systematically identify miRNA targets and provide valuable insights regarding the properties of miRNA effector complexes. ..
- Van Auken K, Weaver D, Edgar L, Wood W. Caenorhabditis elegans embryonic axial patterning requires two recently discovered posterior-group Hox genes. Proc Natl Acad Sci U S A. 2000;97:4499-503 pubmed..K.) 126, 1537-1546]. Therefore, essential embryonic patterning in C. elegans requires only Hox genes of the anterior and posterior paralog groups, raising interesting questions about evolution of the medial-group genes. ..
- Parrish J, Metters H, Chen L, Xue D. Demonstration of the in vivo interaction of key cell death regulators by structure-based design of second-site suppressors. Proc Natl Acad Sci U S A. 2000;97:11916-21 pubmed..The structure-based design of second-site suppressors via homology modeling should be widely applicable for probing important molecular interactions that are implicated in fundamental biological processes. ..
- Buvoli M, Buvoli A, Leinwand L. Effects of pathogenic proline mutations on myosin assembly. J Mol Biol. 2012;415:807-18 pubmed publisher..By showing that the two MPD1 mutations can have dominant effects on distinct components of the contractile apparatus, our data provide the first insights into the pathogenesis of the disease. ..
- Than M, Kudlow B, Han M. Functional analysis of neuronal microRNAs in Caenorhabditis elegans dauer formation by combinational genetics and Neuronal miRISC immunoprecipitation. PLoS Genet. 2013;9:e1003592 pubmed publisher..It also suggests that compromising other aspects of gene expression, along with miRISC, can be an effective approach to reveal miRNA functions in specific tissues under specific physiological conditions. ..
- Weaver B, Weaver Y, Mitani S, Han M. Coupled Caspase and N-End Rule Ligase Activities Allow Recognition and Degradation of Pluripotency Factor LIN-28 during Non-Apoptotic Development. Dev Cell. 2017;41:665-673.e6 pubmed publisher..The interdependence of these proteolytic activities provides a paradigm for non-apoptotic caspase-mediated protein inactivation. ..
- Ventura N, Rea S, Testi R. Long-lived C. elegans mitochondrial mutants as a model for human mitochondrial-associated diseases. Exp Gerontol. 2006;41:974-91 pubmed..The identification of such compensatory pathways opens a window of possibility for future preventative therapies for many HMADs. They may also provide a way of potentially extending human life span. ..
- Cui M, Chen J, Myers T, Hwang B, Sternberg P, Greenwald I, et al. SynMuv genes redundantly inhibit lin-3/EGF expression to prevent inappropriate vulval induction in C. elegans. Dev Cell. 2006;10:667-72 pubmed
- Chen Z, Eastburn D, Han M. The Caenorhabditis elegans nuclear receptor gene nhr-25 regulates epidermal cell development. Mol Cell Biol. 2004;24:7345-58 pubmed..Additionally, our data suggest that nhr-25 may also function with another Hox gene, nob-1, during embryogenesis. Overall, our results indicate that nhr-25 plays an integral role in regulating cellular processes of epidermal cells. ..
- Henderson S, Johnson T. daf-16 integrates developmental and environmental inputs to mediate aging in the nematode Caenorhabditis elegans. Curr Biol. 2001;11:1975-80 pubmed..We suggest that changes in the subcellular localization of DAF-16 by environmental cues allows for rapid reallocation of resources in response to a changing environment at all stages of life. ..
- Parrish J, Yang C, Shen B, Xue D. CRN-1, a Caenorhabditis elegans FEN-1 homologue, cooperates with CPS-6/EndoG to promote apoptotic DNA degradation. EMBO J. 2003;22:3451-60 pubmed..Our results suggest that CRN-1/FEN-1 may play a critical role in switching the state of cells from DNA replication/repair to DNA degradation during apoptosis. ..
- Dostal V, Roberts C, Link C. Genetic mechanisms of coffee extract protection in a Caenorhabditis elegans model of ?-amyloid peptide toxicity. Genetics. 2010;186:857-66 pubmed publisher..These results suggest that the reported protective effects of coffee in multiple neurodegenerative diseases may result from a general activation of the Nrf2 phase II detoxification pathway. ..
- Kapulkin W, Kapulkin V, Hiester B, Link C. Compensatory regulation among ER chaperones in C. elegans. FEBS Lett. 2005;579:3063-8 pubmed..We show that this compensatory regulation is specific for ER chaperones, not dependent on RNA interference, and required for maintaining viability in worms containing a deletion of the hsp-3 gene. ..
- Kniazeva M, Crawford Q, Seiber M, Wang C, Han M. Monomethyl branched-chain fatty acids play an essential role in Caenorhabditis elegans development. PLoS Biol. 2004;2:E257 pubmed..This study exposes unexpected and crucial physiological functions of C15ISO and C17ISO in C. elegans and suggests a potentially important role for mmBCFAs in other eukaryotes. ..
- Skop A, Bergmann D, Mohler W, White J. Completion of cytokinesis in C. elegans requires a brefeldin A-sensitive membrane accumulation at the cleavage furrow apex. Curr Biol. 2001;11:735-46 pubmed
- Chen Z, Han M. C. elegans Rb, NuRD, and Ras regulate lin-39-mediated cell fusion during vulval fate specification. Curr Biol. 2001;11:1874-9 pubmed
- Bergmann D, Lee M, Robertson B, Tsou M, Rose L, Wood W. Embryonic handedness choice in C. elegans involves the Galpha protein GPA-16. Development. 2003;130:5731-40 pubmed..We will henceforth refer to this gene as gpa-16. These results demonstrate for the first time involvement of heterotrimeric G proteins in establishment of embryonic LR asymmetry and suggest how they might act. ..
- Cui M, Kim E, Han M. Diverse chromatin remodeling genes antagonize the Rb-involved SynMuv pathways in C. elegans. PLoS Genet. 2006;2:e74 pubmed..Our findings suggest that multiple chromatin remodeling complexes are involved in regulating the expression of specific genes that play critical roles in developmental decisions. ..
- Kahn N, Rea S, Moyle S, Kell A, Johnson T. Proteasomal dysfunction activates the transcription factor SKN-1 and produces a selective oxidative-stress response in Caenorhabditis elegans. Biochem J. 2008;409:205-13 pubmed
- Cui M, Fay D, Han M. lin-35/Rb cooperates with the SWI/SNF complex to control Caenorhabditis elegans larval development. Genetics. 2004;167:1177-85 pubmed..Our results suggest that LIN-35/Rb and a certain class B synMuv proteins collaborate with the SWI/SNF protein complex to regulate the T cell division as well as other events essential for larval growth...
- Hanna Rose W, Han M. The Caenorhabditis elegans EGL-26 protein mediates vulval cell morphogenesis. Dev Biol. 2002;241:247-58 pubmed..EGL-26 is localized at the apical edge of the vulE cell. It is thus possible that vulE acts to instruct morphological changes in the neighboring cell, vulF, in an interaction mediated by EGL-26. ..
- Seamen E, Blanchette J, Han M. P-type ATPase TAT-2 negatively regulates monomethyl branched-chain fatty acid mediated function in post-embryonic growth and development in C. elegans. PLoS Genet. 2009;5:e1000589 pubmed publisher..This work indicates a novel connection between a P-type ATPase and the critical regulatory function of a specific fatty acid. ..
- Arum O, Johnson T. Reduced expression of the Caenorhabditis elegans p53 ortholog cep-1 results in increased longevity. J Gerontol A Biol Sci Med Sci. 2007;62:951-9 pubmed..This study clarifies the inverse relationship between cep-1 expression and C. elegans life span, and, by extrapolation, that between p53 expression and mammalian life span. ..
- Starr D, Hermann G, Malone C, Fixsen W, Priess J, Horvitz H, et al. unc-83 encodes a novel component of the nuclear envelope and is essential for proper nuclear migration. Development. 2001;128:5039-50 pubmed..We favor a model in which UNC-84 directly recruits UNC-83 to the nuclear envelope where they help transfer force between the cytoskeleton and the nucleus...
- Tang H, Han M. Fatty Acids Regulate Germline Sex Determination through ACS-4-Dependent Myristoylation. Cell. 2017;169:457-469.e13 pubmed publisher..These findings, including a similar role of ACS-4 in a male/female species, uncover a likely conserved mechanism by which FA, an environmental factor, regulates sex determination and reproductive development. ..