Experts and Doctors on transposases in Berkeley, California, United States
Locale: Berkeley, California, United States
Woodhouse M, Freeling M, Lisch D. The mop1 (mediator of paramutation1) mutant progressively reactivates one of the two genes encoded by the MuDR transposon in maize. Genetics. 2006;172:579-92 pubmed
..Conversely, mudrB expression is never restored, its TIR remains methylated, and new insertions of Mu elements are not observed. These data suggest that mudrA and mudrB silencing may be maintained via distinct mechanisms. ..
Laski F, Rubin G. Analysis of the cis-acting requirements for germ-line-specific splicing of the P-element ORF2-ORF3 intron. Genes Dev. 1989;3:720-8 pubmed
..We find that the cis-acting regulatory sequences required for germ line regulation map near to, but not at, the 5' or 3' splice junctions. ..
Zechiedrich E, Khodursky A, Cozzarelli N. Topoisomerase IV, not gyrase, decatenates products of site-specific recombination in Escherichia coli. Genes Dev. 1997;11:2580-92 pubmed
..We also discovered that topo IV has an unexpectedly strong DNA relaxation activity that, together with gyrase and topo I, is able to set the supercoiling levels in Escherichia coli. ..
Hudson M, Lisch D, Quail P. The FHY3 and FAR1 genes encode transposase-related proteins involved in regulation of gene expression by the phytochrome A-signaling pathway. Plant J. 2003;34:453-71 pubmed
..We propose that FAR1 and FHY3 control the expression of their target genes by a mechanism that has evolved directly from the way that an ancestral, MuDRA-like transposase bound to the TIRs of mobile elements. ..
Sabogal A, Lyubimov A, Corn J, Berger J, Rio D. THAP proteins target specific DNA sites through bipartite recognition of adjacent major and minor grooves. Nat Struct Mol Biol. 2010;17:117-23 pubmed publisher
..These findings reveal the conserved mechanisms by which THAP-family proteins engage specific chromosomal target elements. ..
Sabogal A, Rio D. A green fluorescent protein solubility screen in E. coli reveals domain boundaries of the GTP-binding domain in the P element transposase. Protein Sci. 2010;19:2210-8 pubmed publisher
..This study highlights the use of a GFP-based solubility screen on a large multidomain protein to identify highly expressed, soluble truncated domain subregions. ..
Majumdar S, Singh A, Rio D. The human THAP9 gene encodes an active P-element DNA transposase. Science. 2013;339:446-8 pubmed publisher
..Our results indicate that human THAP9 is an active DNA transposase that, although "domesticated," still retains the catalytic activity to mobilize P transposable elements across species. ..
Farboud B, Nix P, Jow M, Gladden J, Meyer B. Molecular antagonism between X-chromosome and autosome signals determines nematode sex. Genes Dev. 2013;27:1159-78 pubmed publisher
..Ironically, while the recent work of others showed that the fly sex signal does not fit this simple paradigm, our work shows that the worm signal does. ..