Genomes and Genes
Experts and Doctors on phosphorylation in Irvine, California, United States
Locale: Irvine, California, United States
- Qin Z, Kaufman R, Khoury R, Khoury M, Aswad D. Isoaspartate accumulation in mouse brain is associated with altered patterns of protein phosphorylation and acetylation, some of which are highly sex-dependent. PLoS ONE. 2013;8:e80758 pubmed publisher..We also uncovered unexpected differences in how male and female mice respond to isoAsp accumulation in the brain. ..
- Tsai C, Ngo B, Tapadia A, Hsu P, Wu G, Lee W. Aurora-A phosphorylates Augmin complex component Hice1 protein at an N-terminal serine/threonine cluster to modulate its microtubule binding activity during spindle assembly. J Biol Chem. 2011;286:30097-106 pubmed publisher..Taken together, these results suggest that Aurora-A modulates the microtubule binding activity of Hice1 in a spatiotemporal manner for proper bipolar spindle assembly. ..
- Clinton L, Blurton Jones M, Myczek K, Trojanowski J, LaFerla F. Synergistic Interactions between Abeta, tau, and alpha-synuclein: acceleration of neuropathology and cognitive decline. J Neurosci. 2010;30:7281-9 pubmed publisher..Together, our data support the notion that Abeta, tau, and alpha-synuclein interact in vivo to promote the aggregation and accumulation of each other and accelerate cognitive dysfunction. ..
- Zhang M, Fu W, Prabhu S, Moore J, Ko J, Kim J, et al. Inhibition of polysome assembly enhances imatinib activity against chronic myelogenous leukemia and overcomes imatinib resistance. Mol Cell Biol. 2008;28:6496-509 pubmed publisher..Together, this work supports the inhibition of translation initiation as a therapeutic strategy for treating cancers fueled by dysregulated translation...
- Wang X, Stanbridge E, Lao X, Cai Q, Fan S, Redpath J. p53-dependent Chk1 phosphorylation is required for maintenance of prolonged G2 Arrest. Radiat Res. 2007;168:706-15 pubmed..The present study suggests the unique role of Chk1 in preventing abrogation of the G2 checkpoint in p53+/+ cells. ..
- Oddo S, Caccamo A, Cheng D, Jouleh B, Torp R, LaFerla F. Genetically augmenting tau levels does not modulate the onset or progression of Abeta pathology in transgenic mice. J Neurochem. 2007;102:1053-63 pubmed
- Li M, Bermak J, Wang Z, Zhou Q. Modulation of dopamine D(2) receptor signaling by actin-binding protein (ABP-280). Mol Pharmacol. 2000;57:446-52 pubmed..Our results suggest a new molecular mechanism of modulating D(2) receptor signaling by cytoskeletal protein interaction. ..
- Nguyen V, Saleh A, Arch A, Yan H, Piazza F, Kim J, et al. Stat2 binding to the interferon-alpha receptor 2 subunit is not required for interferon-alpha signaling. J Biol Chem. 2002;277:9713-21 pubmed
- Shan Y, Yang T, Mestril R, Wang P. Hsp10 and Hsp60 suppress ubiquitination of insulin-like growth factor-1 receptor and augment insulin-like growth factor-1 receptor signaling in cardiac muscle: implications on decreased myocardial protection in diabetic cardiomyopathy. J Biol Chem. 2003;278:45492-8 pubmed..These data suggest that decreased Hsp60 expression and subsequent decline of IGF-1R signaling may be a fundamental mechanism underlying the development of diabetic cardiomyopathy. ..
- Chernyavsky A, Arredondo J, Piser T, Karlsson E, Grando S. Differential coupling of M1 muscarinic and alpha7 nicotinic receptors to inhibition of pemphigus acantholysis. J Biol Chem. 2008;283:3401-8 pubmed..These findings identified novel paradigm of regulation of signaling kinases associated with cholinergic receptors and provided mechanistic explanation of therapeutic activity of cholinomimetics in PV patients. ..
- Bardwell A, Flatauer L, Matsukuma K, Thorner J, Bardwell L. A conserved docking site in MEKs mediates high-affinity binding to MAP kinases and cooperates with a scaffold protein to enhance signal transmission. J Biol Chem. 2001;276:10374-86 pubmed..Thus, both the MEK-MAPK docking interaction and binding to the Ste5 scaffold make mutually reinforcing contributions to the efficiency of signaling by this MAPK cascade in vivo. ..
- Foehr E, Raffioni S, Murray Rust J, Bradshaw R. The role of tyrosine residues in fibroblast growth factor receptor 1 signaling in PC12 cells. Systematic site-directed mutagenesis in the endodomain. J Biol Chem. 2001;276:37529-36 pubmed..However, it does require Tyr(677) and/or Tyr(701), which may function to stabilize the active conformation directly or indirectly. ..
- Wang S, Li Y, Hsu P, Lee S, Kim Y, Lee E. Progesterone receptor A stability is mediated by glycogen synthase kinase-3? in the Brca1-deficient mammary gland. J Biol Chem. 2013;288:26265-74 pubmed publisher..Taken together, these results provide important aspects of tissue specificity of BRCA1-mediated suppression of breast carcinogenesis. ..
- Chabrier M, Blurton Jones M, Agazaryan A, Nerhus J, Martinez Coria H, LaFerla F. Soluble a? promotes wild-type tau pathology in vivo. J Neurosci. 2012;32:17345-50 pubmed publisher..These studies provide critical in vivo evidence for a strong mechanistic link between soluble A?, wild-type tau, and synaptic pathology. ..
- Medeiros R, Kitazawa M, Chabrier M, Cheng D, Baglietto Vargas D, Kling A, et al. Calpain inhibitor A-705253 mitigates Alzheimer's disease-like pathology and cognitive decline in aged 3xTgAD mice. Am J Pathol. 2012;181:616-25 pubmed publisher..Our data provide relevant functional and molecular insights into the beneficial therapeutic effects of inhibiting calpain activation for the management of AD...
- Ho D, Bardwell A, Abdollahi M, Bardwell L. A docking site in MKK4 mediates high affinity binding to JNK MAPKs and competes with similar docking sites in JNK substrates. J Biol Chem. 2003;278:32662-72 pubmed..The MEK1 and MEK2 D-sites displayed a strong selectivity for their cognate MAPK (ERK2) versus a non-cognate MAPK (JNK). In contrast, the MKK4 D-site exhibited only limited selectivity for JNK versus ERK. ..
- Chotiner J, Nielson J, Farris S, Lewandowski G, Huang F, Banos K, et al. Assessment of the role of MAP kinase in mediating activity-dependent transcriptional activation of the immediate early gene Arc/Arg3.1 in the dentate gyrus in vivo. Learn Mem. 2010;17:117-29 pubmed publisher..1 transcription in dentate granule cells in vivo is mediated in part by MAP kinase activation, but other signaling pathways also contribute, especially in the case of Arc/Arg3.1 induction in response to experience. ..
- Sahar S, Zocchi L, Kinoshita C, Borrelli E, Sassone Corsi P. Regulation of BMAL1 protein stability and circadian function by GSK3beta-mediated phosphorylation. PLoS ONE. 2010;5:e8561 pubmed publisher..BMAL1 phosphorylation appears to be an important regulatory step in maintaining the robustness of the circadian clock. ..
- Corbin Lickfett K, Rojas S, Li L, Cocco M, Sandri Goldin R. ICP27 phosphorylation site mutants display altered functional interactions with cellular export factors Aly/REF and TAP/NXF1 but are able to bind herpes simplex virus 1 RNA. J Virol. 2010;84:2212-22 pubmed publisher..These results indicate that these changes in the structure of ICP27 altered in vivo protein interactions that occur in the N terminus but did not prevent RNA binding. ..
- Li L, Johnson L, Dai Ju J, Sandri Goldin R. Hsc70 focus formation at the periphery of HSV-1 transcription sites requires ICP27. PLoS ONE. 2008;3:e1491 pubmed publisher..We propose that one function of the Hsc70 nuclear foci may be to serve to facilitate the process of clearing stalled RNAP II complexes from viral genomes during times of highly active transcription. ..
- Khatiwala C, Kim P, Peyton S, Putnam A. ECM compliance regulates osteogenesis by influencing MAPK signaling downstream of RhoA and ROCK. J Bone Miner Res. 2009;24:886-98 pubmed publisher..These data establish that one possible mechanism by which ECM rigidity regulates osteogenic differentiation involves MAPK activation downstream of the RhoA-ROCK signaling pathway. ..
- Pirino G, Wescott M, Donovan P. Protein kinase A regulates resumption of meiosis by phosphorylation of Cdc25B in mammalian oocytes. Cell Cycle. 2009;8:665-70 pubmed..Our studies suggest that Cdc25B is a direct target of PKA in prophase-arrested oocytes and that Cdc25B phosphorylation results in its inhibition and sequestration by the 14-3-3 protein. ..
- Aiken C, Steffan J, Guerrero C, Khashwji H, Lukacsovich T, Simmons D, et al. Phosphorylation of threonine 3: implications for Huntingtin aggregation and neurotoxicity. J Biol Chem. 2009;284:29427-36 pubmed publisher..Thus, therapeutic strategies that modulate these events could in turn affect Htt pathogenesis. ..
- Sy M, Kitazawa M, Medeiros R, Whitman L, Cheng D, Lane T, et al. Inflammation induced by infection potentiates tau pathological features in transgenic mice. Am J Pathol. 2011;178:2811-22 pubmed publisher..Taken together, infectious agents that trigger central nervous system inflammation may serve as a comorbidity for AD, leading to cognitive impairments by a mechanism that involves exacerbation of tau pathological characteristics. ..
- Hilgenberg L, Pham B, Ortega M, Walid S, Kemmerly T, O Dowd D, et al. Agrin regulation of alpha3 sodium-potassium ATPase activity modulates cardiac myocyte contraction. J Biol Chem. 2009;284:16956-65 pubmed publisher..These data demonstrate that the basal frequency of myocyte contraction depends on endogenous agrin-alpha3 Na,K-ATPase interaction and suggest that agrin modulation of the alpha3 Na,K-ATPase is important in regulating heart function. ..
- Ly C, Arechiga A, Melo J, Walsh C, Ong S. Bcr-Abl kinase modulates the translation regulators ribosomal protein S6 and 4E-BP1 in chronic myelogenous leukemia cells via the mammalian target of rapamycin. Cancer Res. 2003;63:5716-22 pubmed..The mTOR inhibitor rapamycin enhanced imatinib-mediated killing of CML cell lines in vitro, and it overcame imatinib resistance in cells with Bcr-Abl gene amplification. ..
- Anzalone A, Lizardi Ortiz J, Ramos M, De Mei C, Hopf F, Iaccarino C, et al. Dual control of dopamine synthesis and release by presynaptic and postsynaptic dopamine D2 receptors. J Neurosci. 2012;32:9023-34 pubmed publisher..This study reveals previously unappreciated control of DA signaling, shedding new light on region-specific regulation of DA-mediated effects. ..
- Zhao Y, Qin S, Atangan L, Molina Y, Okawa Y, Arpawong H, et al. Casein kinase 1alpha interacts with retinoid X receptor and interferes with agonist-induced apoptosis. J Biol Chem. 2004;279:30844-9 pubmed..Our study shows that CK1alpha can promote cell survival by interfering with RXR agonist-induced apoptosis. Inhibition of CK1alpha may enhance the anti-cancer effects of RXR agonists. ..
- Zan H, Casali P. AID- and Ung-dependent generation of staggered double-strand DNA breaks in immunoglobulin class switch DNA recombination: a post-cleavage role for AID. Mol Immunol. 2008;46:45-61 pubmed publisher..Thus, AID and Ung generate staggered DSBs not only by cleaving intact double-strand DNA, but also by processing blunt DSB ends, whose generation is AID- and Ung-independent, thereby outlining a post-cleavage role for AID in CSR. ..
- Friedrich M, Cui M, Hernandez J, Weist B, Andersen H, Zhang X, et al. Modulation of DRAK2 autophosphorylation by antigen receptor signaling in primary lymphocytes. J Biol Chem. 2007;282:4573-84 pubmed
- Qin S, De Vries G. alpha2 But not alpha1 AMP-activated protein kinase mediates oxidative stress-induced inhibition of retinal pigment epithelium cell phagocytosis of photoreceptor outer segments. J Biol Chem. 2008;283:6744-51 pubmed publisher..Taken together, reduction in POS load under oxidative stress might direct RPE cells to a self-protected status. Thus, activating AMPK could have therapeutic potential in treating dry macular degeneration. ..
- Foehr E, Tatavos A, Tanabe E, Raffioni S, Goetz S, Dimarco E, et al. Discoidin domain receptor 1 (DDR1) signaling in PC12 cells: activation of juxtamembrane domains in PDGFR/DDR/TrkA chimeric receptors. FASEB J. 2000;14:973-81 pubmed..These findings suggest that the DDR1 receptors do have signaling capacity but may require additional components or altered conditions to fully activate their kinase domains and/or sustain the activation of the JM sites. ..
- Wang X, Chen C, Baker P, Chen P, Kaiser P, Huang L. Mass spectrometric characterization of the affinity-purified human 26S proteasome complex. Biochemistry. 2007;46:3553-65 pubmed..The detailed proteomic profiling obtained here is significant to future studies aiming at a complete understanding of the structure-function relationship of the human 26S proteasome complex. ..
- Koike M, Garcia F, Kitazawa M, Green K, LaFerla F. Long term changes in phospho-APP and tau aggregation in the 3xTg-AD mice following cerebral ischemia. Neurosci Lett. 2011;495:55-9 pubmed publisher..Furthermore, we found an increase in insoluble total tau 3-months post-injury. Together these findings further elucidate the long-term impact of cerebral hypoperfusion on Alzheimer's disease. ..
- Reissner K, Paranandi M, Luc T, Doyle H, Mamula M, Lowenson J, et al. Synapsin I is a major endogenous substrate for protein L-isoaspartyl methyltransferase in mammalian brain. J Biol Chem. 2006;281:8389-98 pubmed
- Guo X, Wang X, Wang Z, Banerjee S, Yang J, Huang L, et al. Site-specific proteasome phosphorylation controls cell proliferation and tumorigenesis. Nat Cell Biol. 2016;18:202-12 pubmed publisher..These findings define an important mechanism for proteasome regulation and demonstrate the biological significance of proteasome phosphorylation in regulating cell proliferation and tumorigenesis. ..