Experts and Doctors on genetic complementation test in Berkeley, California, United States


Locale: Berkeley, California, United States
Topic: genetic complementation test

Top Publications

  1. Floor S, Condon K, Sharma D, Jankowsky E, Doudna J. Autoinhibitory Interdomain Interactions and Subfamily-specific Extensions Redefine the Catalytic Core of the Human DEAD-box Protein DDX3. J Biol Chem. 2016;291:2412-21 pubmed publisher
    ..Taken together, these results redefine the structural and functional core of the DDX3 subfamily of DEAD-box proteins. ..
  2. Kirst H, Garcia Cerdan J, Zurbriggen A, Melis A. Assembly of the light-harvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii requires expression of the TLA2-CpFTSY gene. Plant Physiol. 2012;158:930-45 pubmed publisher
    ..The latter exhibit improved solar energy conversion efficiency and photosynthetic productivity under mass culture and bright sunlight conditions. ..
  3. Ryan K, Taylor J, Bowers L. The BAM complex subunit BamE (SmpA) is required for membrane integrity, stalk growth and normal levels of outer membrane {beta}-barrel proteins in Caulobacter crescentus. Microbiology. 2010;156:742-56 pubmed publisher
    ..Based on these findings, we propose that Caulobacter BamE participates in the assembly of outer membrane beta-barrel proteins, including one or more substrates required for the initiation of stalk biogenesis. ..
  4. Song J, Rabinowitz J. The N-terminal, dehydrogenase/cyclohydrolase domain of yeast cytoplasmic trifunctional C1-tetrahydrofolate synthase requires the C-terminal, synthetase domain for the catalytic activity in vitro. FEBS Lett. 1995;376:229-32 pubmed
    ..These results indicate that the N-terminal D/C domain of C1-THF synthase requires the C-terminal 10-formyl-THF synthetase domain for stable catalytic activity in vitro. ..
  5. Casali N. Escherichia coli host strains. Methods Mol Biol. 2003;235:27-48 pubmed
  6. Apuya N, Yadegari R, Fischer R, Harada J, Goldberg R, Harada J. RASPBERRY3 gene encodes a novel protein important for embryo development. Plant Physiol. 2002;129:691-705 pubmed
    ..Our experiments demonstrate the important role of the chloroplast, directly or indirectly, in embryo morphogenesis and development. ..
  7. Wagner D, Hoecker U, Quail P. RED1 is necessary for phytochrome B-mediated red light-specific signal transduction in Arabidopsis. Plant Cell. 1997;9:731-43 pubmed
    ..Together with complementation analysis, the data show that red1 is a novel photomorphogenic mutant. The evidence suggests that red1 represents a putative phytochrome signal transduction mutant potentially specific to the phyB pathway. ..
  8. Linderoth N, Ziermann R, Hagg rd Ljungquist E, Christie G, Calendar R. Nucleotide sequence of the DNA packaging and capsid synthesis genes of bacteriophage P2. Nucleic Acids Res. 1991;19:7207-14 pubmed
    ..coli from a plasmid caused production of a Mr 39,000 Da protein that restored Qam34 growth. This sequence analysis found only genes previously known from analysis of conditional-lethal mutations. No new capsid genes were found...
  9. Calderon R, Garc a Cerd n J, Malno A, Cook R, Russell J, Gaw C, et al. A conserved rubredoxin is necessary for photosystem II accumulation in diverse oxygenic photoautotrophs. J Biol Chem. 2013;288:26688-96 pubmed publisher
    ..Taken together, our data suggest that this rubredoxin is necessary for normal PSII activity in a diverse set of organisms that perform oxygenic photosynthesis...

More Information


  1. Shepherd R, Lindow S. Two dissimilar N-acyl-homoserine lactone acylases of Pseudomonas syringae influence colony and biofilm morphology. Appl Environ Microbiol. 2009;75:45-53 pubmed publisher
    ..While AHL acylases might enable P. syringae B728a to degrade signals of competing species and block expression of their QS-dependent traits, these enzymes also play fundamental roles in biofilm formation. ..
  2. Inwood W, Kane S, DiRuggiero J, Robb F, Clark A. The RadB protein from Pyrococcus does not complement E. coli recA mutations in vivo. Mol Genet Genomics. 2001;265:683-6 pubmed
  3. Ehrenhofer Murray A, Gossen M, Pak D, Botchan M, Rine J. Separation of origin recognition complex functions by cross-species complementation. Science. 1995;270:1671-4 pubmed
    ..The silent state mediated by DmOrc2 was epigenetic; it was propagated during mitotic divisions in a relatively stable way, whereas the nonsilent state was metastable. In contrast, the silent state was erased during meiosis. ..
  4. Vinh D, Drubin D. A yeast TCP-1-like protein is required for actin function in vivo. Proc Natl Acad Sci U S A. 1994;91:9116-20 pubmed
    ..Taken together, these findings demonstrate that TCP-1-like proteins are required for actin and tubulin function in vivo. ..
  5. Hiser L, Basson M, Rine J. ERG10 from Saccharomyces cerevisiae encodes acetoacetyl-CoA thiolase. J Biol Chem. 1994;269:31383-9 pubmed
    ..The deduced amino acid sequence was 40-95% identical to acetoacetyl-CoA thiolases from other organisms. This identity included the active site cysteines located at amino acids 91 and 384 in the Erg10 protein. ..
  6. Li L, He Z, Pandey G, Tsuchiya T, Luan S. Functional cloning and characterization of a plant efflux carrier for multidrug and heavy metal detoxification. J Biol Chem. 2002;277:5360-8 pubmed
    ..Further experiments suggest that AtDTX1 is localized in the plasma membrane in plant cells thereby mediating the efflux of plant-derived or exogenous toxic compounds from the cytoplasm. ..
  7. Huq E, Tepperman J, Quail P. GIGANTEA is a nuclear protein involved in phytochrome signaling in Arabidopsis. Proc Natl Acad Sci U S A. 2000;97:9789-94 pubmed
    ..This result is consistent with the proposed role in phyB signaling, given recent evidence that early phy signaling events are nuclear localized. ..
  8. MacRae I, Li F, Zhou K, Cande W, Doudna J. Structure of Dicer and mechanistic implications for RNAi. Cold Spring Harb Symp Quant Biol. 2006;71:73-80 pubmed
    ..Finally, we combine the structure of Dicer with published biochemical data to propose a model for the architecture of the RNA-induced silencing complex (RISC)-loading complex. ..
  9. Barbash D, Cline T. Genetic and molecular analysis of the autosomal component of the primary sex determination signal of Drosophila melanogaster. Genetics. 1995;141:1451-71 pubmed
  10. Holtzman D, Yang S, Drubin D. Synthetic-lethal interactions identify two novel genes, SLA1 and SLA2, that control membrane cytoskeleton assembly in Saccharomyces cerevisiae. J Cell Biol. 1993;122:635-44 pubmed
  11. Xia C, Lu E, Liu H, Du X, Beutler B, Gong X. The role of Vldlr in intraretinal angiogenesis in mice. Invest Ophthalmol Vis Sci. 2011;52:6572-9 pubmed publisher
    ..Thus, the r26 is a loss-of-function Vldlr mutation. Vldlr on the cell surface probably mediates an antiangiogenic signal to prevent retinal endothelial cells from migrating into the photoreceptor cell layer. ..
  12. Gotwals P, Fristrom J. Three neighboring genes interact with the Broad-Complex and the Stubble-stubbloid locus to affect imaginal disc morphogenesis in Drosophila. Genetics. 1991;127:747-59 pubmed
    ..6-map unit interval between the genetic markers speck and Irregular facets and to the cytological region 60C5-6; 60E9-10 at the tip of chromosome 2R. Genetic evidence is consistent with the view that the BR-C regulates blistered. ..
  13. Ma D, Cook D, Alberti M, Pon N, Nikaido H, Hearst J. Molecular cloning and characterization of acrA and acrE genes of Escherichia coli. J Bacteriol. 1993;175:6299-313 pubmed
    ..coli polypeptides, EnvC and EnvD, respectively. Drug susceptibilities in one acrA mutant (N43) and one envCD mutant (PM61) have been determined and compared. Finally, the possible functions of these proteins are discussed. ..
  14. Jung H, Niyogi K. Mutations in Arabidopsis YCF20-like genes affect thermal dissipation of excess absorbed light energy. Planta. 2010;231:923-37 pubmed publisher
    ..The npq6 npq7 double mutant had an additive NPQ defect, indicating that the YCF20 family members in Arabidopsis have overlapping functions affecting thermal dissipation. ..
  15. Schnell R, D Ari L, Foss M, Goodman D, Rine J. Genetic and molecular characterization of suppressors of SIR4 mutations in Saccharomyces cerevisiae. Genetics. 1989;122:29-46 pubmed
    ..SAN1-mediated suppression was neither due to compensatory mutations in interacting proteins, nor to translational missense suppression. SAN1 may act posttranslationally to control the stability or activity of the SIR4 protein. ..
  16. Schild D, Brake A, Kiefer M, Young D, Barr P. Cloning of three human multifunctional de novo purine biosynthetic genes by functional complementation of yeast mutations. Proc Natl Acad Sci U S A. 1990;87:2916-20 pubmed
    ..The cDNA that complemented ade3 (formyltetrahydrofolate synthetase) is different from the recently isolated human cDNA encoding this enzyme and instead appears to encode a related mitochondrial enzyme. ..
  17. Gallagher J, Babiarz J, Teytelman L, Wolfe K, Rine J. Elaboration, diversification and regulation of the Sir1 family of silencing proteins in Saccharomyces. Genetics. 2009;181:1477-91 pubmed publisher
  18. Vogel J, Roth B, Cigan M, Freeling M. Expression of the two maize TATA binding protein genes and function of the encoded TBP proteins by complementation in yeast. Plant Cell. 1993;5:1627-38 pubmed
    ..This is a conclusive demonstration of in vivo activity for a nonyeast TBP protein, and these complementation results point to particular amino acids in TBP that are likely to influence species-specific protein interactions. ..
  19. Gee S, Krauss S, Miller E, Aoyagi K, Arenas J, Conboy J. Cloning of mDEAH9, a putative RNA helicase and mammalian homologue of Saccharomyces cerevisiae splicing factor Prp43. Proc Natl Acad Sci U S A. 1997;94:11803-7 pubmed
    ..Our results provide additional support for the hypothesis that the spliceosomal machinery that mediates regulated, dynamic changes in conformation of pre-mRNA and snRNP RNAs has been highly conserved through evolution. ..
  20. Fu H, Luan S. AtKuP1: a dual-affinity K+ transporter from Arabidopsis. Plant Cell. 1998;10:63-73 pubmed
    ..We conclude that the AtKUP1 gene product may function as a K+ transporter in Arabidopsis roots over a broad range of [K+] in the soil. ..
  21. Yalovsky S, Kulukian A, Rodríguez Concepción M, Young C, Gruissem W. Functional requirement of plant farnesyltransferase during development in Arabidopsis. Plant Cell. 2000;12:1267-78 pubmed
    ..Together, these results provide a basis for future studies on the involvement of FTase in specific developmental processes and for structure-function analysis of FTase in vivo. ..
  22. Williams S, Senaratne R, Mougous J, Riley L, Bertozzi C. 5'-adenosinephosphosulfate lies at a metabolic branch point in mycobacteria. J Biol Chem. 2002;277:32606-15 pubmed
    ..coli, confirming the ability of this organism to make PAPS. The expression of recombinant M. tuberculosis APS kinase provides a means for the discovery of inhibitors of this enzyme and thus of the biosynthesis of SL-1. ..
  23. Sandler S, Chackerian B, Li J, Clark A. Sequence and complementation analysis of recF genes from Escherichia coli, Salmonella typhimurium, Pseudomonas putida and Bacillus subtilis: evidence for an essential phosphate binding loop. Nucleic Acids Res. 1992;20:839-45 pubmed
    ..Lastly we made two chimeric recF genes by exchanging the amino terminal 48 amino acids of the S. typhimurium and E. coli recF genes. Both chimeras could complement E. coli chromosomal recF mutations. ..
  24. Soupene E, Chu T, Corbin R, Hunt D, Kustu S. Gas channels for NH(3): proteins from hyperthermophiles complement an Escherichia coli mutant. J Bacteriol. 2002;184:3396-400 pubmed
    ..Despite low expression levels, His-tagged Aquifex Amt could be purified by heating and nickel chelate affinity chromatography. It could be studied genetically in Escherichia coli. ..
  25. Choi T, Wagner J, Jackson A. Sequence analysis of the trailer region of sonchus yellow net virus genomic RNA. Virology. 1994;202:33-40 pubmed
    ..In this regard, SYNV differs from vesicular stomatitis virus and is similar to other rhabdoviruses which also fail to accumulate detectable minus-strand leader RNAs. ..
  26. Fish R, Ammerman M, Davie J, Lu B, Pham C, Howe L, et al. Genetic interactions between TFIIF and TFIIS. Genetics. 2006;173:1871-84 pubmed
    ..Together, these results suggest a physiologically relevant functional connection between TFIIS and TFIIF. ..
  27. Wiese C, Hinz J, Tebbs R, Nham P, Urbin S, Collins D, et al. Disparate requirements for the Walker A and B ATPase motifs of human RAD51D in homologous recombination. Nucleic Acids Res. 2006;34:2833-43 pubmed
    ..We present a model in which ATPase sites are formed in a bipartite manner between RAD51D and other RAD51 paralogs. ..
  28. Mayfield J, Davies M, Dimster Denk D, Pleskac N, McCarthy S, Boydston E, et al. Surrogate genetics and metabolic profiling for characterization of human disease alleles. Genetics. 2012;190:1309-23 pubmed publisher
  29. Linderoth N, Julien B, Flick K, Calendar R, Christie G. Molecular cloning and characterization of bacteriophage P2 genes R and S involved in tail completion. Virology. 1994;200:347-59 pubmed
    ..We propose that R and S are tail completion proteins that are essential for stable head joining...
  30. Rudner D, Kanaar R, Breger K, Rio D. Mutations in the small subunit of the Drosophila U2AF splicing factor cause lethality and developmental defects. Proc Natl Acad Sci U S A. 1996;93:10333-7 pubmed
    ..Consistent with a general role in splicing, a null allele of dU2AF38 is fully penetrant recessive lethal, like null alleles of the Drosophila U2AF large subunit. ..
  31. Bayer C, von Kalm L, Fristrom J. Relationships between protein isoforms and genetic functions demonstrate functional redundancy at the Broad-Complex during Drosophila metamorphosis. Dev Biol. 1997;187:267-82 pubmed
    ..The establishment of these relationships between BR-C genetic functions and protein isoforms is an important step toward understanding the roles of BR-C proteins in directing metamorphic responses to ecdysone. ..
  32. Elkins C, Nikaido H. Chimeric analysis of AcrA function reveals the importance of its C-terminal domain in its interaction with the AcrB multidrug efflux pump. J Bacteriol. 2003;185:5349-56 pubmed
    ..We conclude that a small region of AcrA close to, but not at, its C terminus is involved in the interaction with its cognate pump protein, AcrB. ..
  33. Inouye C, Dhillon N, Thorner J. Ste5 RING-H2 domain: role in Ste4-promoted oligomerization for yeast pheromone signaling. Science. 1997;278:103-6 pubmed
    ..Thus, the RING-H2 domain mediates Ste4-Ste5 interaction, which is a prerequisite for Ste5-Ste5 self-association and signaling. ..
  34. Heimer S, Evans D, Stern M, Barbieri J, YAHR T, Fleiszig S. Pseudomonas aeruginosa utilizes the type III secreted toxin ExoS to avoid acidified compartments within epithelial cells. PLoS ONE. 2013;8:e73111 pubmed publisher
    ..They also show that variability in ExoS expression by wild-type PA inside cells can differentially influence the fate of individual intracellular bacteria, even within the same cell...
  35. Kirst H, Garcia Cerdan J, Zurbriggen A, Ruehle T, Melis A. Truncated photosystem chlorophyll antenna size in the green microalga Chlamydomonas reinhardtii upon deletion of the TLA3-CpSRP43 gene. Plant Physiol. 2012;160:2251-60 pubmed publisher
    ..The latter exhibit improved solar energy conversion efficiency and photosynthetic productivity under mass culture and bright sunlight conditions...
  36. Singhvi A, Frank C, Garriga G. The T-box gene tbx-2, the homeobox gene egl-5 and the asymmetric cell division gene ham-1 specify neural fate in the HSN/PHB lineage. Genetics. 2008;179:887-98 pubmed publisher
    ..Expression of EGL-5 in the HSN/PHB precursor but not in the PHB neuron suggests that EGL-5 specifies precursor fate. These observations reveal a role for both EGL-5 and TBX-2 in neural fate specification in the HSN/PHB lineage. ..
  37. Vinh D, Welch M, Corsi A, Wertman K, Drubin D. Genetic evidence for functional interactions between actin noncomplementing (Anc) gene products and actin cytoskeletal proteins in Saccharomyces cerevisiae. Genetics. 1993;135:275-86 pubmed
    ..Finally, we report the results of tests of two models previously proposed to explain extragenic noncomplementation. ..
  38. Sauer J, Sotelo Troha K, von Moltke J, Monroe K, Rae C, Brubaker S, et al. The N-ethyl-N-nitrosourea-induced Goldenticket mouse mutant reveals an essential function of Sting in the in vivo interferon response to Listeria monocytogenes and cyclic dinucleotides. Infect Immun. 2011;79:688-94 pubmed publisher
    ..Additionally, Sting is required for the response to c-di-GMP and L. monocytogenes in vivo. Our results provide new functions for Sting in the innate interferon response to pathogens. ..
  39. Parsons H, Christiansen K, Knierim B, Carroll A, Ito J, Batth T, et al. Isolation and proteomic characterization of the Arabidopsis Golgi defines functional and novel components involved in plant cell wall biosynthesis. Plant Physiol. 2012;159:12-26 pubmed publisher
    ..Overall, these data show key proteins involved in primary cell wall synthesis and include a mixture of well-characterized and unknown proteins whose biological roles and importance as targets for future research can now be realized...
  40. Karim F, Chang H, Therrien M, Wassarman D, Laverty T, Rubin G. A screen for genes that function downstream of Ras1 during Drosophila eye development. Genetics. 1996;143:315-29 pubmed
    ..At least two suppressors appear to function either between Ras1 and Raf or in parallel to Raf. ..
  41. de Souza A, Ionescu M, Baccari C, da Silva A, Lindow S. Phenotype overlap in Xylella fastidiosa is controlled by the cyclic di-GMP phosphodiesterase Eal in response to antibiotic exposure and diffusible signal factor-mediated cell-cell signaling. Appl Environ Microbiol. 2013;79:3444-54 pubmed publisher
    ..However, the effect of cell density is dominant compared to that of antibiotic stress, since eal is suppressed by RpfF, which may prevent inappropriate behavioral changes in response to antibiotic stress when DSF accumulates. ..
  42. Ziermann R, Bartlett B, Calendar R, Christie G. Functions involved in bacteriophage P2-induced host cell lysis and identification of a new tail gene. J Bacteriol. 1994;176:4974-84 pubmed
    ..Gene X must encode a tail protein, since lysates from nonpermissive cells infected with the X amber mutant were complemented in vitro by similar lysates of cells infected with P2 head mutants but not with tail mutants...
  43. Forrester W, Perens E, Zallen J, Garriga G. Identification of Caenorhabditis elegans genes required for neuronal differentiation and migration. Genetics. 1998;148:151-65 pubmed
    ..Null mutants also fail to express CEH-10, suggesting that CEH-10 regulates its own expression. Finally, we found that ceh-10 is necessary for the differentiation of AIY and RMED, two additional cells that express CEH-10. ..
  44. Enquist Newman M, Faust A, Bravo D, Santos C, Raisner R, Hanel A, et al. Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform. Nature. 2014;505:239-43 pubmed publisher
    ..These results show that all major sugars in brown macroalgae can be used as feedstocks for biofuels and value-added renewable chemicals in a manner that is comparable to traditional arable-land-based feedstocks. ..
  45. Le H, Donaldson K, Cook K, Karpen G. A high proportion of genes involved in position effect variegation also affect chromosome inheritance. Chromosoma. 2004;112:269-76 pubmed
  46. Therrien M, Morrison D, Wong A, Rubin G. A genetic screen for modifiers of a kinase suppressor of Ras-dependent rough eye phenotype in Drosophila. Genetics. 2000;156:1231-42 pubmed
    ..One of them corresponds to the kismet locus, which encodes a putative chromatin remodeling factor. The relevance of these loci with respect to the function of KSR and the Ras1 pathway in general is discussed. ..
  47. Harrison S, Solomon N, Rubin G. A genetic analysis of the 63E-64A genomic region of Drosophila melanogaster: identification of mutations in a replication factor C subunit. Genetics. 1995;139:1701-9 pubmed
    ..In addition we have isolated 11 new mutant alleles of the disembodied gene. ..
  48. Chen Y, Holcomb C, Moore H. Expression and localization of two low molecular weight GTP-binding proteins, Rab8 and Rab10, by epitope tag. Proc Natl Acad Sci U S A. 1993;90:6508-12 pubmed
    ..This approach should provide a general tool for the analyses of other members of the YPT/SEC4/rab gene family. ..
  49. Cutforth T, Rubin G. Mutations in Hsp83 and cdc37 impair signaling by the sevenless receptor tyrosine kinase in Drosophila. Cell. 1994;77:1027-36 pubmed
    ..Together, these data support a role for Hsp90 proteins in tyrosine kinase regulation and suggest that signals promoting neuronal differentiation may involve cell cycle control. ..
  50. Windler S, Bilder D. Endocytic internalization routes required for delta/notch signaling. Curr Biol. 2010;20:538-43 pubmed publisher
    ..The absolute requirement for dynamin-dependent endocytosis but not endosomal entry or Rab11 activity supports "pulling force" rather than "recycling" models for Delta activation. ..
  51. Nakano A, Brada D, Schekman R. A membrane glycoprotein, Sec12p, required for protein transport from the endoplasmic reticulum to the Golgi apparatus in yeast. J Cell Biol. 1988;107:851-63 pubmed
    ..In the process of transport, Sec12p itself may migrate to the Golgi apparatus and function in subsequent transport events. ..
  52. Menut L, Vaccari T, Dionne H, Hill J, Wu G, Bilder D. A mosaic genetic screen for Drosophila neoplastic tumor suppressor genes based on defective pupation. Genetics. 2007;177:1667-77 pubmed
    ..The isolation of additional loci that affect hyperplastic as well as neoplastic growth indicates the utility of this screening strategy for studying epithelial growth control. ..
  53. Ashby M, Errada P, Boyartchuk V, Rine J. Isolation and DNA sequence of the STE14 gene encoding farnesyl cysteine: carboxyl methyltransferase. Yeast. 1993;9:907-13 pubmed
    ..In comparisons of the C-terminal methyltransferase amino acid sequence with those in the PIR and Swiss protein databases, no significantly similar sequences were found nor were conserved regions from other methyltransferases present. ..
  54. Peers G, Truong T, Ostendorf E, Busch A, Elrad D, Grossman A, et al. An ancient light-harvesting protein is critical for the regulation of algal photosynthesis. Nature. 2009;462:518-21 pubmed publisher
    ..Thus, these data indicate that plants and algae use different proteins to dissipate harmful excess light energy and protect the photosynthetic apparatus from damage. ..
  55. Protas M, Patel N. Evolution of coloration patterns. Annu Rev Cell Dev Biol. 2008;24:425-46 pubmed publisher
  56. Fairchild C, Schumaker M, Quail P. HFR1 encodes an atypical bHLH protein that acts in phytochrome A signal transduction. Genes Dev. 2000;14:2377-91 pubmed
    ..HFR1 mRNA is 30-fold more abundant in FRc than in continuous red light, suggesting a potential mechanistic basis for the specificity of HFR1 to phyA signaling. ..
  57. Li L, Tutone A, Drummond R, Gardner R, Luan S. A novel family of magnesium transport genes in Arabidopsis. Plant Cell. 2001;13:2761-75 pubmed
    ..Using green fluorescence protein as a reporter, we localized AtMGT1 protein to the plasma membrane in Arabidopsis plants. We suggest that the AtMGT gene family encodes a Mg(2+) transport system in higher plants. ..