Genomes and Genes
Experts and Doctors on amino acid sequence in Berkeley, California, United States
Locale: Berkeley, California, United States
Topic: amino acid sequence
Publications314 found, 100 shown here
- Corbo J, Erives A, Di Gregorio A, Chang A, Levine M. Dorsoventral patterning of the vertebrate neural tube is conserved in a protochordate. Development. 1997;124:2335-44 pubmed..We propose that precursors of the floor plate and neural crest were present in a common ancestor of both vertebrates and ascidians...
- Barth A, Dugas J, Ngai J. Noncoordinate expression of odorant receptor genes tightly linked in the zebrafish genome. Neuron. 1997;19:359-69 pubmed..Furthermore, genes from this cluster are activated at different times of development. Together, these results indicate that genomically linked odorant receptor genes are not coordinately regulated. ..
- Nurrish S, Segalat L, Kaplan J. Serotonin inhibition of synaptic transmission: Galpha(0) decreases the abundance of UNC-13 at release sites. Neuron. 1999;24:231-42 pubmed
- Lai L, Yokota H, Hung L, Kim R, Kim S. Crystal structure of archaeal RNase HII: a homologue of human major RNase H. Structure. 2000;8:897-904 pubmed..The involvement of the unique C-terminal domain in substrate recognition explains the different reaction specificity observed between the two classes of RNase H...
- Cheeseman I, Enquist Newman M, MULLER REICHERT T, Drubin D, Barnes G. Mitotic spindle integrity and kinetochore function linked by the Duo1p/Dam1p complex. J Cell Biol. 2001;152:197-212 pubmed..In addition, Duo1p and Dam1p localize to kinetochores in chromosome spreads, suggesting that this complex may serve as a link between the kinetochore and the mitotic spindle. ..
- Labourier E, Adams M, Rio D. Modulation of P-element pre-mRNA splicing by a direct interaction between PSI and U1 snRNP 70K protein. Mol Cell. 2001;8:363-73 pubmed..These data show that the processing of specific target transcripts, such as the P-element mRNA, is regulated by a functional PSI-U1 snRNP interaction in Drosophila. ..
- Fish R, Kane C. Promoting elongation with transcript cleavage stimulatory factors. Biochim Biophys Acta. 2002;1577:287-307 pubmed..However, there are yet many unanswered questions regarding the regulation of these factors and their effects on target genes. ..
- Zang X, Loke P, Kim J, Murphy K, Waitz R, Allison J. B7x: a widely expressed B7 family member that inhibits T cell activation. Proc Natl Acad Sci U S A. 2003;100:10388-92 pubmed..These studies identify a costimulatory pathway that may have a unique function in downregulation of tissue-specific autoimmunity and antitumor responses. ..
- Chen S, Shin D, Pufan R, Kim R, Kim S. Crystal structure of methenyltetrahydrofolate synthetase from Mycoplasma pneumoniae (GI: 13508087) at 2.2 A resolution. Proteins. 2004;56:839-43 pubmed
- Rosenberg O, Deindl S, Sung R, Nairn A, Kuriyan J. Structure of the autoinhibited kinase domain of CaMKII and SAXS analysis of the holoenzyme. Cell. 2005;123:849-60 pubmed
- Young S, Diolaiti D, Conacci Sorrell M, Ruiz Trillo I, Eisenman R, King N. Premetazoan ancestry of the Myc-Max network. Mol Biol Evol. 2011;28:2961-71 pubmed publisher..Furthermore, core features of metazoan Myc function, including heterodimerization with Max, binding to E-box sequences in DNA, and localization to the nucleus, predate the origin of metazoans. ..
- Chou S, Krasileva K, Holton J, Steinbrenner A, Alber T, Staskawicz B. Hyaloperonospora arabidopsidis ATR1 effector is a repeat protein with distributed recognition surfaces. Proc Natl Acad Sci U S A. 2011;108:13323-8 pubmed publisher..These results suggest that ATR1 is a modular repeat protein belonging to an ancient family of oomycete effectors that rapidly evolves to escape host detection and adopt diverse virulence functions. ..
- Tam C, Mun J, Evans D, Fleiszig S. Cytokeratins mediate epithelial innate defense through their antimicrobial properties. J Clin Invest. 2012;122:3665-77 pubmed publisher..These data suggest that epithelial cytokeratins function as endogenous antimicrobial peptides in the host defense against infection and that keratin-derived antimicrobials may serve as effective therapeutic agents. ..
- Xia C, Chang B, DeRosa A, Cheng C, White T, Gong X. Cataracts and microphthalmia caused by a Gja8 mutation in extracellular loop 2. PLoS ONE. 2012;7:e52894 pubmed publisher..This work provides molecular insights to understand the cataract and microphthalmia/microcornea phenotype caused by Gja8 mutations in mice and humans. ..
- Hosobuchi M, Kreis T, Schekman R. SEC21 is a gene required for ER to Golgi protein transport that encodes a subunit of a yeast coatomer. Nature. 1992;360:603-5 pubmed..These observations demonstrate that a non-clathrin coat protein plays an essential role in intercompartmental transport. ..
- Nose A, Mahajan V, Goodman C. Connectin: a homophilic cell adhesion molecule expressed on a subset of muscles and the motoneurons that innervate them in Drosophila. Cell. 1992;70:553-67 pubmed..During synapse formation, the protein localizes to synaptic sites; afterward, it largely disappears. Thus, connectin is a novel cell adhesion molecule whose expression suggests a role in target recognition. ..
- Paine Saunders S, Fristrom D, Fristrom J. The Drosophila IMP-E2 gene encodes an apically secreted protein expressed during imaginal disc morphogenesis. Dev Biol. 1990;140:337-51 pubmed..Although the temporal and spatial expression of the IMP-E2 gene are consistent with a role in disc morphogenesis, its specific functions remain to be determined. ..
- Perkins K, Admon A, Patel N, Tjian R. The Drosophila Fos-related AP-1 protein is a developmentally regulated transcription factor. Genes Dev. 1990;4:822-34 pubmed..These results indicate that dFRA is a developmentally regulated transcription factor and suggest that its potential interplay with dJRA plays an important role in cell-type-specific transcription during Drosophila embryonic development. ..
- Carthew R, Rubin G. seven in absentia, a gene required for specification of R7 cell fate in the Drosophila eye. Cell. 1990;63:561-77 pubmed..These data indicate that the sina gene product is necessary at a stage in the determination of R7 cell fate when R7 receives and interprets developmental signals from neighboring cells, and possibly acts by regulating gene expression. ..
- Courchesne W, Kunisawa R, Thorner J. A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae. Cell. 1989;58:1107-19 pubmed..pombe) gene products. The recovery-promoting activity of the KSS1 gene requires a functional WHI1 gene, which encodes a yeast homolog to animal cyclins, suggesting that the KSS1 and WHI1 proteins act in the same growth control pathway. ..
- Duden R, Hosobuchi M, Hamamoto S, Winey M, Byers B, Schekman R. Yeast beta- and beta'-coat proteins (COP). Two coatomer subunits essential for endoplasmic reticulum-to-Golgi protein traffic. J Biol Chem. 1994;269:24486-95 pubmed..Genetic interactions connect sec27-1 and sec21-1 (coatomer gamma subunit) with the ARF1 and ARF2 genes and with the SEC22, BET1, and BOS1 genes, which encode membrane proteins involved in ER-to-Golgi transport. ..
- Akasaka K, Frudakis T, Killian C, George N, Yamasu K, Khaner O, et al. Genomic organization of a gene encoding the spicule matrix protein SM30 in the sea urchin Strongylocentrotus purpuratus. J Biol Chem. 1994;269:20592-8 pubmed..We also demonstrate that 2.6 kilobases of upstream sequence of SM30-alpha are sufficient to direct primary mesenchyme cell-specific expression of a reporter gene construct. ..
- Webster M, Goya L, Ge Y, Maiyar A, Firestone G. Characterization of sgk, a novel member of the serine/threonine protein kinase gene family which is transcriptionally induced by glucocorticoids and serum. Mol Cell Biol. 1993;13:2031-40 pubmed
- Dynlacht B, Weinzierl R, Admon A, Tjian R. The dTAFII80 subunit of Drosophila TFIID contains beta-transducin repeats. Nature. 1993;363:176-9 pubmed..These findings suggest the formation of TFIID may require an ordered assembly of the TAFs, some of which bind directly to TBP and others that are tethered to the complex as a result of specific TAF/TAF interactions. ..
- Lambert K, Allen K, Sussex I. Cloning and characterization of an esophageal-gland-specific chorismate mutase from the phytoparasitic nematode Meloidogyne javanica. Mol Plant Microbe Interact. 1999;12:328-36 pubmed..Therefore, we hypothesize that this CM is involved in allowing nematodes to parasitize plants. ..
- Brazer S, Williams H, Chappell T, Cande W. A fission yeast kinesin affects Golgi membrane recycling. Yeast. 2000;16:149-66 pubmed..We conclude that Klp3 plays a role in BFA-induced membrane transport. The nucleotide sequence of S. pombe klp3(+) was submitted to GenBank under Accession No. AF154055. ..
- Kim K, Cheong Y, Gupta R, Luan S. Interaction specificity of Arabidopsis calcineurin B-like calcium sensors and their target kinases. Plant Physiol. 2000;124:1844-53 pubmed..These results may provide structural basis for the functional specificity of CBL family of calcium sensors and their targets. ..
- Shin D, Yokota H, Kim R, Kim S. Crystal structure of conserved hypothetical protein Aq1575 from Aquifex aeolicus. Proc Natl Acad Sci U S A. 2002;99:7980-5 pubmed..There are several highly conserved residues in these putative active sites. The structure based molecular properties and thermostability of the protein are discussed. ..
- Shin D, Lou Y, Jancarik J, Yokota H, Kim R, Kim S. Crystal structure of YjeQ from Thermotoga maritima contains a circularly permuted GTPase domain. Proc Natl Acad Sci U S A. 2004;101:13198-203 pubmed..The overall structural features of TmYjeQ make it a good candidate for an RNA-binding protein, which is consistent with the biochemical data of the YjeQ subfamily in binding to the ribosome. ..
- Carrico I, Carlson B, Bertozzi C. Introducing genetically encoded aldehydes into proteins. Nat Chem Biol. 2007;3:321-2 pubmed..This genetically encoded 'aldehyde tag' is no larger than a His(6) tag and can be exploited for numerous protein labeling applications. ..
- Wang H, Long S, Ciferri C, Westermann S, Drubin D, Barnes G, et al. Architecture and flexibility of the yeast Ndc80 kinetochore complex. J Mol Biol. 2008;383:894-903 pubmed publisher..We hypothesize that the kink in Ndc80 is essential for correct kinetochore geometry and could be part of a tension-sensing mechanism at the kinetochore. ..
- Ratcliff K, Corn J, Marqusee S. Structure, stability, and folding of ribonuclease H1 from the moderately thermophilic Chlorobium tepidum: comparison with thermophilic and mesophilic homologues. Biochemistry. 2009;48:5890-8 pubmed publisher..It should now be possible to design RNases H that display the desired thermophilic or mesophilic properties, as defined by their DeltaC(p) values, and therefore fine-tune the energy landscape in a predictable fashion...
- Inwood W, Hall J, Kim K, Fong R, Kustu S. Genetic evidence for an essential oscillation of transmembrane-spanning segment 5 in the Escherichia coli ammonium channel AmtB. Genetics. 2009;183:1341-55 pubmed publisher..Movement of TM 5 appears to control the opening of both the periplasmic entry and the cytoplasmic exit to the pore. ..
- Sullivan B, Coscoy L. The U24 protein from human herpesvirus 6 and 7 affects endocytic recycling. J Virol. 2010;84:1265-75 pubmed publisher..Inhibition of a basic cellular process by U24 has interesting implications not only for the pathogenicity of roseoloviruses but also for our understanding of the biology of endosomal transport...
- Hartung S, Arvai A, Wood T, Kolappan S, Shin D, Craig L, et al. Ultrahigh resolution and full-length pilin structures with insights for filament assembly, pathogenic functions, and vaccine potential. J Biol Chem. 2011;286:44254-65 pubmed publisher..tularensis virulence. Combined results define a unified pilin architecture, specialized subdomain roles in pilus assembly and function, and potential therapeutic targets...
- Calderon R, Garc a Cerd n J, Malno A, Cook R, Russell J, Gaw C, et al. A conserved rubredoxin is necessary for photosystem II accumulation in diverse oxygenic photoautotrophs. J Biol Chem. 2013;288:26688-96 pubmed publisher..Taken together, our data suggest that this rubredoxin is necessary for normal PSII activity in a diverse set of organisms that perform oxygenic photosynthesis...
- Conboy J, Chan J, Chasis J, Kan Y, Mohandas N. Tissue- and development-specific alternative RNA splicing regulates expression of multiple isoforms of erythroid membrane protein 4.1. J Biol Chem. 1991;266:8273-80 pubmed..Developmentally controlled alternative RNA splicing in the spectrin-actin-binding region may help regulate remodeling of membrane architecture and mechanical properties that occur during erythropoiesis. ..
- Schnell R, D Ari L, Foss M, Goodman D, Rine J. Genetic and molecular characterization of suppressors of SIR4 mutations in Saccharomyces cerevisiae. Genetics. 1989;122:29-46 pubmed..SAN1-mediated suppression was neither due to compensatory mutations in interacting proteins, nor to translational missense suppression. SAN1 may act posttranslationally to control the stability or activity of the SIR4 protein. ..
- Bazan J, Fletterick R. Detection of a trypsin-like serine protease domain in flaviviruses and pestiviruses. Virology. 1989;171:637-9 pubmed..Structural modeling of the putative flaviviral protease domain suggests amino acids that are crucial for catalytic activity and substrate binding...
- Kadonaga J, Carner K, Masiarz F, Tjian R. Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain. Cell. 1987;51:1079-90 pubmed..To facilitate the identification of mutant variants of Sp1 that are defective in DNA binding, we have also devised a bacterial colony assay for detection of Sp1 binding to DNA. ..
- Chen J, Attardi L, Verrijzer C, Yokomori K, Tjian R. Assembly of recombinant TFIID reveals differential coactivator requirements for distinct transcriptional activators. Cell. 1994;79:93-105 pubmed..These results establish that specific subcomplexes of TFIID can mediate activation by different classes of activators and suggest that TAFs perform multiple functions during activation. ..
- Hiser L, Basson M, Rine J. ERG10 from Saccharomyces cerevisiae encodes acetoacetyl-CoA thiolase. J Biol Chem. 1994;269:31383-9 pubmed..The deduced amino acid sequence was 40-95% identical to acetoacetyl-CoA thiolases from other organisms. This identity included the active site cysteines located at amino acids 91 and 384 in the Erg10 protein. ..
- Goodrich J, Hoey T, Thut C, Admon A, Tjian R. Drosophila TAFII40 interacts with both a VP16 activation domain and the basal transcription factor TFIIB. Cell. 1993;75:519-30 pubmed..These results, taken together with previous studies of Sp1 and dTAFII110, establish that different activators interact with distinct TAFs in the TFIID complex and that TAFs can contact both activators and basal factors. ..
- Yokomori K, Chen J, Admon A, Zhou S, Tjian R. Molecular cloning and characterization of dTAFII30 alpha and dTAFII30 beta: two small subunits of Drosophila TFIID. Genes Dev. 1993;7:2587-97 pubmed..Our studies suggest that both of the small subunits of TFIID play a role in the assembly of the complex and may contribute to the stability of multiple TAF-TAF interactions. ..
- Lichtsteiner S, Tjian R. Cloning and properties of the Caenorhabditis elegans TATA-box-binding protein. Proc Natl Acad Sci U S A. 1993;90:9673-7 pubmed..elegans nuclear extracts. CeTBP is therefore a basal transcription factor. ..
- Julien B, Lefevre P, Calendar R. The two P2 Ogr-like domains of the delta protein from bacteriophage P4 are required for activity. Virology. 1997;230:292-9 pubmed..A monomric Delta from another satellite phage, phi R73, can function efficiently as a covalent dimer but when this Delta is made dimeric with the second half of P4 delta, it activates less efficiently...
- Hanke T, Corral L, Vance R, Raulet D. 2F1 antigen, the mouse homolog of the rat "mast cell function-associated antigen", is a lectin-like type II transmembrane receptor expressed by natural killer cells. Eur J Immunol. 1998;28:4409-17 pubmed..Instead, we find that approximately 40% of mouse NK cells express 2F1-Ag. Thus, MAFA/2F1-Ag may modulate immunological responses on at least two different cell types bridging the specific and innate immune system. ..
- Baum P, Guenther C, Frank C, Pham B, Garriga G. The Caenorhabditis elegans gene ham-2 links Hox patterning to migration of the HSN motor neuron. Genes Dev. 1999;13:472-83 pubmed
- Stroschein S, Bonni S, Wrana J, Luo K. Smad3 recruits the anaphase-promoting complex for ubiquitination and degradation of SnoN. Genes Dev. 2001;15:2822-36 pubmed..Our studies elucidate an important mechanism and pathway for the degradation of SnoN and more importantly, reveal a novel role of the APC in the regulation of TGF-beta signaling. ..
- Zhuang R, Lin M, Napoli J. cis-Retinol/androgen dehydrogenase, isozyme 3 (CRAD3): a short-chain dehydrogenase active in a reconstituted path of 9-cis-retinoic acid biosynthesis in intact cells. Biochemistry. 2002;41:3477-83 pubmed..These data are consistent with the participation of CRAD3 in the biogeneration of 9-cis-retinoic acid. ..
- Huang S, Rio D, Marletta M. Ligand binding and inhibition of an oxygen-sensitive soluble guanylate cyclase, Gyc-88E, from Drosophila. Biochemistry. 2007;46:15115-22 pubmed..82 +/- 0.15 mM under anaerobic conditions. The Ki for oxygen was calculated to be 51 +/- 28 microM. The biochemical properties of Gyc-88E are unique for guanylate cyclases and suggest a possible function as an oxygen sensor. ..
- McNally R, Bowman G, Goedken E, O Donnell M, Kuriyan J. Analysis of the role of PCNA-DNA contacts during clamp loading. BMC Struct Biol. 2010;10:3 pubmed publisher..The interaction of DNA is not, however, restricted to one orientation, as indicated by analysis of the PCNA-DNA co-crystals. ..
- Chen H, Attieh Z, Syed B, Kuo Y, Stevens V, Fuqua B, et al. Identification of zyklopen, a new member of the vertebrate multicopper ferroxidase family, and characterization in rodents and human cells. J Nutr. 2010;140:1728-35 pubmed publisher..Knockdown with small interfering RNA in BeWo cells indicates that this gene represents the previously detected oxidase. We propose calling this new member of the MCF family "zyklopen." ..
- Oyeyemi O, Sours K, Lee T, Kohen A, Resing K, Ahn N, et al. Comparative hydrogen-deuterium exchange for a mesophilic vs thermophilic dihydrofolate reductase at 25 °C: identification of a single active site region with enhanced flexibility in the mesophilic protein. Biochemistry. 2011;50:8251-60 pubmed publisher..The region with increased flexibility in Ec-DHFR corresponds to one of two regions previously proposed to control the enthalpic barrier for hydride transfer in Bs-DHFR [Oyeyemi et al. (2010) Proc. Natl. Acad. Sci. U.S.A. 107, 10074]. ..
- Strycharska M, Arias Palomo E, Lyubimov A, Erzberger J, O Shea V, BUSTAMANTE C, et al. Nucleotide and partner-protein control of bacterial replicative helicase structure and function. Mol Cell. 2013;52:844-54 pubmed publisher..Our findings establish the DnaB collar as an autoregulatory hub that controls the ability of the helicase to transition between different functional states in response to both nucleotide and replication initiation/elongation factors. ..
- Janes S, Palcic M, Scaman C, Smith A, Brown D, Dooley D, et al. Identification of topaquinone and its consensus sequence in copper amine oxidases. Biochemistry. 1992;31:12147-54 pubmed..2) and at 575-587 nm in basic solution (1-2 M KOH). These spectral properties, which appear unique to topaquinone, provide a rapid and simple test for the presence of this cofactor in intact enzymes.(ABSTRACT TRUNCATED AT 250 WORDS) ..
- Wedeen C, Price D, Weisblat D. Cloning and sequencing of a leech homolog to the Drosophila engrailed gene. FEBS Lett. 1991;279:300-2 pubmed..These are found within the second and third of the three putative helices of the homeodomain, and in both of the intervening turn regions. ..
- Tong L, de Vos A, Milburn M, Kim S. Crystal structures at 2.2 A resolution of the catalytic domains of normal ras protein and an oncogenic mutant complexed with GDP. J Mol Biol. 1991;217:503-16 pubmed..The structural similarity between ras protein and the bacterial elongation factor Tu suggests that their common structural motif might be conserved for other guanine nucleotide binding proteins. ..
- Sanders D, Gillece Castro B, Stock A, Burlingame A, Koshland D. Identification of the site of phosphorylation of the chemotaxis response regulator protein, CheY. J Biol Chem. 1989;264:21770-8 pubmed..The duration of the signal can be controlled by small structural elements within the phosphorylated protein. ..
- Bieber A, Snow P, Hortsch M, Patel N, Jacobs J, Traquina Z, et al. Drosophila neuroglian: a member of the immunoglobulin superfamily with extensive homology to the vertebrate neural adhesion molecule L1. Cell. 1989;59:447-60 pubmed..We report here on the identification of a lethal mutation in the neuroglian gene. ..
- Johnson T, Yee B, Carlson D, Buchanan B, Johnson R, Mathews W, et al. Thioredoxin from Rhodospirillum rubrum: primary structure and relation to thioredoxins from other photosynthetic bacteria. J Bacteriol. 1988;170:2406-8 pubmed..The results indicate that R. rubrum has an NADP-thioredoxin system similar to that of other photosynthetic purple bacteria. ..
- Kammen H, Marvel C, Hardy L, Penhoet E. Purification, structure, and properties of Escherichia coli tRNA pseudouridine synthase I. J Biol Chem. 1988;263:2255-63 pubmed
- Lincoln C, Long J, Yamaguchi J, Serikawa K, Hake S. A knotted1-like homeobox gene in Arabidopsis is expressed in the vegetative meristem and dramatically alters leaf morphology when overexpressed in transgenic plants. Plant Cell. 1994;6:1859-76 pubmed..The expression pattern of KNAT1 in the shoot meristem combined with the results of transgenic overexpression experiments supports the hypothesis that class 1 kn1-like genes play a role in morphogenesis. ..
- Machin N, Lee J, Barnes G. Microtubule stability in budding yeast: characterization and dosage suppression of a benomyl-dependent tubulin mutant. Mol Biol Cell. 1995;6:1241-59 pubmed..As tub2-404 mutants are also defective for spindle elongation, this provides additional support for a role for JSN1 during anaphase...
- Keck J, Marqusee S. The putative substrate recognition loop of Escherichia coli ribonuclease H is not essential for activity. J Biol Chem. 1996;271:19883-7 pubmed..coli homologue. Surprisingly, the resulting mutant protein is active in Mn2+-dependent fashion. Therefore, the basic helix/loop is not required for RNase H activity. ..
- Machin N, Lee J, Chamany K, Barnes G. Dosage suppressors of a benomyl-dependent tubulin mutant: evidence for a link between microtubule stability and cellular metabolism. Genetics. 1996;144:1363-73 pubmed
- Nonet M, Staunton J, Kilgard M, Fergestad T, Hartwieg E, Horvitz H, et al. Caenorhabditis elegans rab-3 mutant synapses exhibit impaired function and are partially depleted of vesicles. J Neurosci. 1997;17:8061-73 pubmed..On the basis of the morphological defects at neuromuscular junctions, we postulate that RAB-3 may regulate recruitment of vesicles to the active zone or sequestration of vesicles near release sites. ..
- Zito K, Fetter R, Goodman C, Isacoff E. Synaptic clustering of Fascilin II and Shaker: essential targeting sequences and role of Dlg. Neuron. 1997;19:1007-16 pubmed..Finally, transient expression studies show that the pathway these proteins take to the synapse involves either an active clustering or a selective stabilization in the synaptic membrane. ..
- Yalovsky S, Rodríguez Concepción M, Bracha K, Toledo Ortiz G, Gruissem W. Prenylation of the floral transcription factor APETALA1 modulates its function. Plant Cell. 2000;12:1257-66 pubmed..Together, the results demonstrate that AP1 is a target of farnesyltransferase and suggest that farnesylation alters the function and perhaps specificity of the transcription factor. ..
- Malarkannan S, Horng T, Eden P, Gonzalez F, Shih P, Brouwenstijn N, et al. Differences that matter: major cytotoxic T cell-stimulating minor histocompatibility antigens. Immunity. 2000;13:333-44 pubmed..B immune response. The immunodominance of these antigens can be explained by their differential transcription in the donor versus the host strains and their expression in professional donor antigen-presenting cells. ..
- Weiss E, Bishop A, Shokat K, Drubin D. Chemical genetic analysis of the budding-yeast p21-activated kinase Cla4p. Nat Cell Biol. 2000;2:677-85 pubmed..Our data indicate that CLA4 PAK activity is required at an early stage of budding, after actin polarization and coincident with formation of the septin ring, for early bud morphogenesis and assembly of a cytokinesis site. ..
- Dose A, Hillman D, Wong C, Sohlberg L, Lin Jones J, Burnside B. Myo3A, one of two class III myosin genes expressed in vertebrate retina, is localized to the calycal processes of rod and cone photoreceptors and is expressed in the sacculus. Mol Biol Cell. 2003;14:1058-73 pubmed..The numerous IQ motifs may play a structural or signaling role in the Myo3A, and its localization to calycal processes indicates that this myosin mediates a local function at this site in vertebrate photoreceptors. ..
- Diefenbach A, Hsia J, Hsiung M, Raulet D. A novel ligand for the NKG2D receptor activates NK cells and macrophages and induces tumor immunity. Eur J Immunol. 2003;33:381-91 pubmed..Tumor cells ectopically expressing the molecule were efficiently rejected by naive mice, and induced strong protective immunity to the parental, ligand-negative tumor cells. ..
- Smith H, Hake S. The interaction of two homeobox genes, BREVIPEDICELLUS and PENNYWISE, regulates internode patterning in the Arabidopsis inflorescence. Plant Cell. 2003;15:1717-27 pubmed..The physical association of the PNY and BP proteins suggests that they participate in a complex that regulates early patterning events in the inflorescence meristem. ..
- Doucleff M, Pelton J, Lee P, Nixon B, Wemmer D. Structural basis of DNA recognition by the alternative sigma-factor, sigma54. J Mol Biol. 2007;369:1070-8 pubmed publisher..This structure elucidates the basis for sequence specific recognition of the -24 element, orients sigma(54) on the promoter, and suggests how the C-terminal domain of sigma(54) interacts with RNAP...
- Shen Y, Bonnot F, Imsand E, RoseFigura J, Sjolander K, Klinman J. Distribution and properties of the genes encoding the biosynthesis of the bacterial cofactor, pyrroloquinoline quinone. Biochemistry. 2012;51:2265-75 pubmed publisher..These studies propose previously unidentified roles for several of the gene products, as well as identifying possible new targets for antibiotic design and application. ..
- Kim H, Nikaido H. Different functions of MdtB and MdtC subunits in the heterotrimeric efflux transporter MdtB(2)C complex of Escherichia coli. Biochemistry. 2012;51:4188-97 pubmed publisher..Three-dimensional models of MdtB and MdtC, based on sequence homology with the AcrB transporter, also support this interpretation. ..
- Bond Watts B, Weeks A, Chang M. Biochemical and structural characterization of the trans-enoyl-CoA reductase from Treponema denticola. Biochemistry. 2012;51:6827-37 pubmed
- Reindl S, Ghosh A, Williams G, Lassak K, Neiner T, Henche A, et al. Insights into FlaI functions in archaeal motor assembly and motility from structures, conformations, and genetics. Mol Cell. 2013;49:1069-82 pubmed publisher..Collective structural and mutational results identify in vivo functional components for assembly and motility, phosphate-triggered rearrangements by ATP hydrolysis, and molecular predictors for distinct ATPase superfamily functions...
- Gaul U, Mardon G, Rubin G. A putative Ras GTPase activating protein acts as a negative regulator of signaling by the Sevenless receptor tyrosine kinase. Cell. 1992;68:1007-19 pubmed..Our results suggest that Gap1 acts as a negative regulator of signaling by Sevenless by down-regulating the activity of the Ras1 protein, which has been shown to be a key element in signaling by Sevenless. ..
- Mirel D, Lustre V, Chamberlin M. An operon of Bacillus subtilis motility genes transcribed by the sigma D form of RNA polymerase. J Bacteriol. 1992;174:4197-204 pubmed..coli and Salmonella typhimurium, and these factors appear to be representative of a family of factors implicated in flagellar synthesis in many bacterial species, which we propose to designate the sigma 28 family. ..
- Cyert M, Kunisawa R, Kaim D, Thorner J. Yeast has homologs (CNA1 and CNA2 gene products) of mammalian calcineurin, a calmodulin-regulated phosphoprotein phosphatase. Proc Natl Acad Sci U S A. 1991;88:7376-80 pubmed..Thus, calcineurin action antagonizes the mating-pheromone response pathway. ..
- Hemmati Brivanlou A, de la Torre J, Holt C, Harland R. Cephalic expression and molecular characterization of Xenopus En-2. Development. 1991;111:715-24 pubmed..In addition to the main band of expression at the midbrain-hindbrain boundary, we show that the protein is expressed in three novel areas: the mandibular arch, the optic tectum and the region of anterior pituitary. ..
- Gloss L, Planas A, Kirsch J. Contribution to catalysis and stability of the five cysteines in Escherichia coli aspartate aminotransferase. Preparation and properties of a cysteine-free enzyme. Biochemistry. 1992;31:32-9 pubmed..The changes manifested in the mutation of the conserved Cys-191 to alanine are no greater than those observed with the four nonconserved cysteines. We consider the evolutionary implications of these findings. ..
- Heilig J, Freeman M, Laverty T, Lee K, Campos A, Rubin G, et al. Isolation and characterization of the disconnected gene of Drosophila melanogaster. EMBO J. 1991;10:809-15 pubmed..Mobilization of a P-element inserted near the gene resulted in the deletion of the 5' end of the gene and produced flies indistinguishable from those carrying the disco allele. ..
- Mimura C, Admon A, Hurt K, Ames G. The nucleotide-binding site of HisP, a membrane protein of the histidine permease. Identification of amino acid residues photoaffinity labeled by 8-azido-ATP. J Biol Chem. 1990;265:19535-42 pubmed..These consensus motifs are found in many purine nucleotide-binding proteins. The relationship between the location of these residues and the overall structure of the ATP-binding site is discussed...