Experts and Doctors on saccharomyces cerevisiae proteins in Basel, Basel City, Switzerland


Locale: Basel, Basel City, Switzerland
Topic: saccharomyces cerevisiae proteins

Top Publications

  1. Martin G, Keller W. Mutational analysis of mammalian poly(A) polymerase identifies a region for primer binding and catalytic domain, homologous to the family X polymerases, and to other nucleotidyltransferases. EMBO J. 1996;15:2593-603 pubmed
    ..This homology extends as far as cca: tRNA nucleotidyltransferase and streptomycin adenylyltransferase, an antibiotic resistance factor. ..
  2. Hoepfner D, Karkare S, Helliwell S, Pfeifer M, Trunzer M, De Bonnechose S, et al. An integrated approach for identification and target validation of antifungal compounds active against Erg11p. Antimicrob Agents Chemother. 2012;56:4233-40 pubmed publisher
    ..Thus, we have identified non-azole Erg11p inhibitors, using a systematic approach for ligand and target characterization. ..
  3. Hegnauer A, Hustedt N, Shimada K, Pike B, Vogel M, Amsler P, et al. An N-terminal acidic region of Sgs1 interacts with Rpa70 and recruits Rad53 kinase to stalled forks. EMBO J. 2012;31:3768-83 pubmed publisher
    ..We propose that the recruitment of Rad53 by phosphorylated Sgs1 promotes the replication checkpoint response on HU. Loss of the R1 domain increases lethality selectively in cells lacking Mus81, Slx4, Slx5 or Slx8. ..
  4. Krek W. Proteolysis and the G1-S transition: the SCF connection. Curr Opin Genet Dev. 1998;8:36-42 pubmed
    ..The constituents of SCFs are members of evolutionary conserved protein families. SCF-based ubiquitination pathways may play a key role in diverse biological processes, such as cell proliferation, differentiation and development. ..
  5. Huranová M, Muruganandam G, Weiss M, Spang A. Dynamic assembly of the exomer secretory vesicle cargo adaptor subunits. EMBO Rep. 2016;17:202-19 pubmed publisher
    ..The multifactorial assembly pathway results in an exquisitely fine-tuned adaptor complex, enabling the cell to quickly respond and adapt to changes such as stress. ..
  6. Dion V, Kalck V, Seeber A, Schleker T, Gasser S. Cohesin and the nucleolus constrain the mobility of spontaneous repair foci. EMBO Rep. 2013;14:984-91 pubmed publisher
    ..We show that cohesin and nucleolar integrity constrain the mobility of these foci, consistent with the notion that spontaneous, S-phase damage is preferentially repaired from the sister chromatid. ..
  7. Munder T, Furst P. The Saccharomyces cerevisiae CDC25 gene product binds specifically to catalytically inactive ras proteins in vivo. Mol Cell Biol. 1992;12:2091-9 pubmed
    ..Cdc25 binds predominantly to the catalytically inactive GDP-bound form of Ras2, whereas a conformational change of Ras2 to its activated GTP-bound state results in its loss of binding affinity to Cdc25. ..
  8. Nagai S, Dubrana K, Tsai Pflugfelder M, Davidson M, Roberts T, Brown G, et al. Functional targeting of DNA damage to a nuclear pore-associated SUMO-dependent ubiquitin ligase. Science. 2008;322:597-602 pubmed publisher
    ..This suggests that strand breaks are shunted to nuclear pores for a repair pathway controlled by a conserved SUMO-dependent E3 ligase. ..
  9. San Paolo S, Vanacova S, Schenk L, Scherrer T, Blank D, Keller W, et al. Distinct roles of non-canonical poly(A) polymerases in RNA metabolism. PLoS Genet. 2009;5:e1000555 pubmed publisher
    ..This indicates widespread and integrative functions of TRAMP complexes for the coordination of different gene expression regulatory processes. ..

More Information


  1. Walter D, Matter A, Fahrenkrog B. Loss of histone H3 methylation at lysine 4 triggers apoptosis in Saccharomyces cerevisiae. PLoS Genet. 2014;10:e1004095 pubmed publisher
    ..cerevisiae. Given the evolutionary conservation of H3K4 methylation this likely plays a role in apoptosis regulation in a wide range of organisms...
  2. Molinier J, Stamm M, Hohn B. SNM-dependent recombinational repair of oxidatively induced DNA damage in Arabidopsis thaliana. EMBO Rep. 2004;5:994-9 pubmed
    ..Therefore, our results suggest the existence, in plants, of a novel SNM-dependent recombinational repair process of oxidatively induced DNA damage. ..
  3. Ferreira H, Luke B, Schober H, Kalck V, Lingner J, Gasser S. The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast. Nat Cell Biol. 2011;13:867-74 pubmed publisher
    ..We propose that SUMO-dependent association with the nuclear periphery restrains bound telomerase, whereas active elongation correlates with telomere release. ..
  4. Schlatter I, Meira M, Ueberschlag V, Hoepfner D, Movva R, Hynes N. MHO1, an evolutionarily conserved gene, is synthetic lethal with PLC1; Mho1p has a role in invasive growth. PLoS ONE. 2012;7:e32501 pubmed publisher
    ..Moreover, a role for Memo in cell motility/invasion appears to be conserved across species. ..
  5. Rockenbauch U, Ritz A, Sacristan C, Roncero C, Spang A. The complex interactions of Chs5p, the ChAPs, and the cargo Chs3p. Mol Biol Cell. 2012;23:4402-15 pubmed publisher
    ..The cargo Chs3p probably also uses a complex motif for the interaction with Chs6, as the C-terminus of Chs3p interacts with Chs6p and is necessary, but not sufficient, for TGN export. ..
  6. Shimobayashi M, Oppliger W, Moes S, Jeno P, Hall M. TORC1-regulated protein kinase Npr1 phosphorylates Orm to stimulate complex sphingolipid synthesis. Mol Biol Cell. 2013;24:870-81 pubmed publisher
    ..Thus activation of Orm and complex sphingolipid synthesis upon TORC1 inhibition is a physiological response to starvation. ..
  7. Ozonov E, van Nimwegen E. Nucleosome free regions in yeast promoters result from competitive binding of transcription factors that interact with chromatin modifiers. PLoS Comput Biol. 2013;9:e1003181 pubmed publisher
    ..Together our results imply that nucleosome free regions in yeast promoters results from the binding of a specific class of TFs that recruit chromatin remodelers...
  8. Kueng S, Oppikofer M, Gasser S. SIR proteins and the assembly of silent chromatin in budding yeast. Annu Rev Genet. 2013;47:275-306 pubmed publisher
    ..Here, we integrate genetic, structural, and cell biological data into an updated overview of yeast silent chromatin assembly. ..
  9. Shimada K, Gasser S. DNA replication: Pif1 pulls the plug on stalled replication forks. Curr Biol. 2012;22:R404-5 pubmed publisher
    ..Findings in fission yeast extend evidence from budding yeast, and argue for universal mechanisms that ensure replication integrity. ..
  10. Kueng S, Tsai Pflugfelder M, Oppikofer M, Ferreira H, Roberts E, Tsai C, et al. Regulating repression: roles for the sir4 N-terminus in linker DNA protection and stabilization of epigenetic states. PLoS Genet. 2012;8:e1002727 pubmed publisher
  11. Schleker T, Shimada K, Sack R, Pike B, Gasser S. Cell cycle-dependent phosphorylation of Rad53 kinase by Cdc5 and Cdc28 modulates checkpoint adaptation. Cell Cycle. 2010;9:350-63 pubmed
    ..We thus demonstrate that cell cycle-dependent phosphorylation can fine-tune the response of Rad53 to DNA damage. ..
  12. Varela E, Shimada K, Laroche T, Leroy D, Gasser S. Lte1, Cdc14 and MEN-controlled Cdk inactivation in yeast coordinate rDNA decompaction with late telophase progression. EMBO J. 2009;28:1562-75 pubmed publisher
    ..This may argue that different levels of Cdk inactivation control spindle disassembly and chromatin decompaction. Mutation of lte1 also impaired rotation of the nucleus in early G1. ..
  13. Junne T, Schwede T, Goder V, Spiess M. Mutations in the Sec61p channel affecting signal sequence recognition and membrane protein topology. J Biol Chem. 2007;282:33201-9 pubmed
    ..Our results suggest a model in which the regulated opening of the translocon is required for the faithful orientation of membrane proteins. ..
  14. van Attikum H, Fritsch O, Gasser S. Distinct roles for SWR1 and INO80 chromatin remodeling complexes at chromosomal double-strand breaks. EMBO J. 2007;26:4113-25 pubmed
    ..Thus, these two related chromatin remodelers have distinct roles in DSB repair and checkpoint activation. ..
  15. Hungerbuehler A, Philippsen P, Gladfelter A. Limited functional redundancy and oscillation of cyclins in multinucleated Ashbya gossypii fungal cells. Eukaryot Cell. 2007;6:473-86 pubmed
    ..We propose that the cohabitation of different cyclins in nuclei has led to enhanced substrate specificity and limited functional redundancy within classes of cyclins in multinucleated cells. ..
  16. Lisztwan J, Marti A, Sutterlüty H, Gstaiger M, Wirbelauer C, Krek W. Association of human CUL-1 and ubiquitin-conjugating enzyme CDC34 with the F-box protein p45(SKP2): evidence for evolutionary conservation in the subunit composition of the CDC34-SCF pathway. EMBO J. 1998;17:368-83 pubmed
    ..The dependency of p45(SKP2)-p19(SKP1) complex formation on cyclin A-CDK2 may ensure tight coordination of the activities of the cell cycle clock with those of a potential ubiquitin conjugation pathway. ..
  17. Morales Johansson H, Jenoe P, Cooke F, Hall M. Negative regulation of phosphatidylinositol 4,5-bisphosphate levels by the INP51-associated proteins TAX4 and IRS4. J Biol Chem. 2004;279:39604-10 pubmed
    ..The interaction between TAX4/IRS4 and INP51 is analogous to the association of EPS15 with the 5-phosphatase synaptojanin 1 in mammalian cells, suggesting that EPS15 is an activator of synaptojanin 1. ..
  18. Bocquet N, Bizard A, Abdulrahman W, Larsen N, Faty M, Cavadini S, et al. Structural and mechanistic insight into Holliday-junction dissolution by topoisomerase III? and RMI1. Nat Struct Mol Biol. 2014;21:261-8 pubmed publisher
    ..Our results provide a mechanistic rationale for how RMI1 stabilizes TopIII?-gate opening to enable dissolution and illustrate how binding partners modulate topoisomerase function. ..
  19. Jacinto E, Guo B, Arndt K, Schmelzle T, Hall M. TIP41 interacts with TAP42 and negatively regulates the TOR signaling pathway. Mol Cell. 2001;8:1017-26 pubmed
  20. Schmidt A, Schmelzle T, Hall M. The RHO1-GAPs SAC7, BEM2 and BAG7 control distinct RHO1 functions in Saccharomyces cerevisiae. Mol Microbiol. 2002;45:1433-41 pubmed
    ..This suggests that different RHO1GAPs control different RHO1 effector pathways, thus ensuring their individual regulation at the appropriate place and time. ..
  21. Morsomme P, Prescianotto Baschong C, Riezman H. The ER v-SNAREs are required for GPI-anchored protein sorting from other secretory proteins upon exit from the ER. J Cell Biol. 2003;162:403-12 pubmed
    ..Therefore, we propose that v-SNAREs are part of the cargo protein sorting machinery upon exit from the ER and that a correct sorting process is necessary for proper maturation of GPI-anchored proteins. ..
  22. Vogel K, Hörz W, Hinnen A. The two positively acting regulatory proteins PHO2 and PHO4 physically interact with PHO5 upstream activation regions. Mol Cell Biol. 1989;9:2050-7 pubmed
    ..Gel retardation assays with the PHO2 protein revealed a binding region that lay between the two PHO4-binding sites. DNase I footprint analysis suggested a PHO2-binding site covering the region between -277 and -296. ..
  23. Shi T, Bunker R, Mattarocci S, Ribeyre C, Faty M, Gut H, et al. Rif1 and Rif2 shape telomere function and architecture through multivalent Rap1 interactions. Cell. 2013;153:1340-53 pubmed publisher
    ..This molecular Velcro relies on Rif1 and Rif2 to recruit and stabilize Rap1 on telomeric arrays and is required for telomere homeostasis in vivo. ..
  24. Rohner S, Kalck V, Wang X, Ikegami K, Lieb J, Gasser S, et al. Promoter- and RNA polymerase II-dependent hsp-16 gene association with nuclear pores in Caenorhabditis elegans. J Cell Biol. 2013;200:589-604 pubmed publisher
  25. Mattarocci S, Reinert J, Bunker R, Fontana G, Shi T, Klein D, et al. Rif1 maintains telomeres and mediates DNA repair by encasing DNA ends. Nat Struct Mol Biol. 2017;24:588-595 pubmed publisher
    ..We propose that tight associations between the Rif1-NTD and DNA gate access of processing factors to DNA ends, enabling Rif1 to mediate diverse telomere maintenance and DNA repair functions. ..
  26. Stoecklin G, Colombi M, Raineri I, Leuenberger S, Mallaun M, Schmidlin M, et al. Functional cloning of BRF1, a regulator of ARE-dependent mRNA turnover. EMBO J. 2002;21:4709-18 pubmed
    ..This approach, which identified BRF1 as an essential regulator of ARE-dependent mRNA decay, should also be applicable to other cis-elements of mRNA turnover. ..
  27. Hunkeler M, Stuttfeld E, Hagmann A, Imseng S, Maier T. The dynamic organization of fungal acetyl-CoA carboxylase. Nat Commun. 2016;7:11196 pubmed publisher
    ..In contrast to related carboxylases, large-scale conformational changes are required for substrate turnover, and are mediated by the CD under phosphorylation control. ..
  28. Dames S, Mulet J, Rathgeb Szabo K, Hall M, Grzesiek S. The solution structure of the FATC domain of the protein kinase target of rapamycin suggests a role for redox-dependent structural and cellular stability. J Biol Chem. 2005;280:20558-64 pubmed
    ..Because the amount of TOR mRNA is not changed, the redox state of the FATC disulfide bond is probably influencing the degradation of TOR. ..
  29. Vanacova S, Wolf J, Martin G, Blank D, Dettwiler S, Friedlein A, et al. A new yeast poly(A) polymerase complex involved in RNA quality control. PLoS Biol. 2005;3:e189 pubmed
    ..This polyadenylation-mediated RNA surveillance resembles the role of polyadenylation in bacterial RNA turnover. ..
  30. Shimada K, Filipuzzi I, Stahl M, Helliwell S, Studer C, Hoepfner D, et al. TORC2 signaling pathway guarantees genome stability in the face of DNA strand breaks. Mol Cell. 2013;51:829-39 pubmed publisher
    ..These phenocopy TORC2 inhibition on Zeocin, although modulation of calcineurin-sensitive transcription does not. These results implicate TORC2-mediated actin filament regulation in the survival of low levels of DNA damage. ..
  31. Walter D, Wissing S, Madeo F, Fahrenkrog B. The inhibitor-of-apoptosis protein Bir1p protects against apoptosis in S. cerevisiae and is a substrate for the yeast homologue of Omi/HtrA2. J Cell Sci. 2006;119:1843-51 pubmed
    ..Therefore, Bir1p, like its closest metazoan homologues deterin and survivin, has dual functions: it participates in chromosome segregation events and cytokinesis and exhibits anti-apoptotic activity. ..
  32. Dirnberger D, Seuwen K. Signaling of human frizzled receptors to the mating pathway in yeast. PLoS ONE. 2007;2:e954 pubmed
    ..Our data demonstrate that Frizzled receptors can functionally replace mating factor receptors in yeast and offer an experimental system to study modulators of Frizzled receptors. ..
  33. Grosshans B, Novick P. Identification and verification of Sro7p as an effector of the Sec4p Rab GTPase. Methods Enzymol. 2008;438:95-108 pubmed publisher
    ..We also describe the methods used to identify and verify one candidate, Sro7p, as a bona fide Sec4p effector. This includes tests of the specificity and efficiency of binding both in vitro and in vivo. ..
  34. Martin G, Keller W. RNA-specific ribonucleotidyl transferases. RNA. 2007;13:1834-49 pubmed
    ..2'-5'Oligo(A) synthetases differ from the other rNTrs by synthesizing oligonucleotides with 2'-5'-phosphodiester bonds de novo. ..
  35. Gladfelter A, Hungerbuehler A, Philippsen P. Asynchronous nuclear division cycles in multinucleated cells. J Cell Biol. 2006;172:347-62 pubmed
    ..We hypothesize that the continuous cytoplasm in these cells promoted the evolution of a nuclear division cycle in which CDK inhibitors primarily control CDK activity rather than oscillating mitotic cyclin proteins. ..
  36. Fritsch O, Benvenuto G, Bowler C, Molinier J, Hohn B. The INO80 protein controls homologous recombination in Arabidopsis thaliana. Mol Cell. 2004;16:479-85 pubmed
    ..However, the transcriptional regulation of repair-related genes is unaffected in the mutant. This suggests a dual role for INO80 in transcription and DNA repair by HR. ..
  37. Crespo J, Helliwell S, Wiederkehr C, Demougin P, Fowler B, Primig M, et al. NPR1 kinase and RSP5-BUL1/2 ubiquitin ligase control GLN3-dependent transcription in Saccharomyces cerevisiae. J Biol Chem. 2004;279:37512-7 pubmed
    ..Thus, NPR1 and BUL1/2 antagonistically control GLN3-dependent transcription, suggesting a role for regulated ubiquitination in the control of nutrient-responsive transcription. ..
  38. Fahrenkrog B, Sauder U, Aebi U. The S. cerevisiae HtrA-like protein Nma111p is a nuclear serine protease that mediates yeast apoptosis. J Cell Sci. 2004;117:115-26 pubmed
    ..By contrast, overexpression of Nma111p enhances apoptotic-like cell death. Therefore, Nma111p, like its mammalian homologue HtrA2, mediates apoptosis. ..
  39. Niimi T, Clements J, Gehring W, Callaerts P. Dominant-negative form of the Pax6 homolog eyeless for tissue-specific loss-of-function studies in the developing eye and brain in drosophila. Genesis. 2002;34:74-5 pubmed
  40. van Lookeren Campagne M, Gill R. Tumor-suppressor p53 is expressed in proliferating and newly formed neurons of the embryonic and postnatal rat brain: comparison with expression of the cell cycle regulators p21Waf1/Cip1, p27Kip1, p57Kip2, p16Ink4a, cyclin G1, and the proto-oncogene . J Comp Neurol. 1998;397:181-98 pubmed
    ..The nonoverlapping temporal and spatial expression patterns of p53 and its transcriptional targets Bax, cyclin G1 and p21Waf1 suggest that each of these gene products fulfill independent roles in brain morphogenesis. ..
  41. Deshpande I, Seeber A, Shimada K, Keusch J, Gut H, Gasser S. Structural Basis of Mec1-Ddc2-RPA Assembly and Activation on Single-Stranded DNA at Sites of Damage. Mol Cell. 2017;68:431-445.e5 pubmed publisher