Experts and Doctors on saccharomyces cerevisiae proteins in Umeå, Västerbotten, Sweden


Locale: Umeå, Västerbotten, Sweden
Topic: saccharomyces cerevisiae proteins

Top Publications

  1. Chilkova O, Stenlund P, Isoz I, Stith C, Grabowski P, Lundström E, et al. The eukaryotic leading and lagging strand DNA polymerases are loaded onto primer-ends via separate mechanisms but have comparable processivity in the presence of PCNA. Nucleic Acids Res. 2007;35:6588-97 pubmed
    ..We conclude that Pol epsilon and Pol delta exhibit comparable processivity, but are loaded on the primer-end via different mechanisms. ..
  2. Balagopal V, Fluch L, Nissan T. Ways and means of eukaryotic mRNA decay. Biochim Biophys Acta. 2012;1819:593-603 pubmed publisher
    ..This review will discuss in detail the recent developments in the regulation of mRNA decay focusing on yeast as a model system. This article is part of a Special Issue entitled: Nuclear Transport and RNA Processing. ..
  3. Tsaponina O, Barsoum E, Aström S, Chabes A. Ixr1 is required for the expression of the ribonucleotide reductase Rnr1 and maintenance of dNTP pools. PLoS Genet. 2011;7:e1002061 pubmed publisher
    ..A reduction of the histone gene dosage in the rad53 mutant restores Ixr1 levels. Our results demonstrate that Ixr1, but not Dun1, is required for the proper RNR1 expression both during an unperturbed cell cycle and after DNA damage. ..
  4. Gan H, Yu C, Devbhandari S, Sharma S, Han J, Chabes A, et al. Checkpoint Kinase Rad53 Couples Leading- and Lagging-Strand DNA Synthesis under Replication Stress. Mol Cell. 2017;68:446-455.e3 pubmed publisher
    ..Therefore, we propose that Rad53 prevents the generation of excessive ssDNA under replication stress by coordinating DNA unwinding with synthesis of both strands. ..
  5. Kochenova O, Bezalel Buch R, Tran P, Makarova A, Chabes A, Burgers P, et al. Yeast DNA polymerase ? maintains consistent activity and mutagenicity across a wide range of physiological dNTP concentrations. Nucleic Acids Res. 2017;45:1200-1218 pubmed publisher
    ..The results support a model wherein dNTP elevation is needed to facilitate non-mutagenic tolerance pathways, while Pol? synthesis represents a unique mechanism of rescuing stalled replication when dNTP supply is low. ..
  6. Zhou Y, Chen C, Johansson M. The pre-mRNA retention and splicing complex controls tRNA maturation by promoting TAN1 expression. Nucleic Acids Res. 2013;41:5669-78 pubmed publisher
    ..Our results demonstrate that TAN1 pre-mRNA processing has an unprecedented requirement for RES factors and that the complex controls the formation of ac(4)C in tRNA. ..
  7. Aksenova A, Volkov K, Maceluch J, Pursell Z, Rogozin I, Kunkel T, et al. Mismatch repair-independent increase in spontaneous mutagenesis in yeast lacking non-essential subunits of DNA polymerase ?. PLoS Genet. 2010;6:e1001209 pubmed publisher
  8. Pozdnyakova I, Wittung Stafshede P. Non-linear effects of macromolecular crowding on enzymatic activity of multi-copper oxidase. Biochim Biophys Acta. 2010;1804:740-4 pubmed publisher
    ..The observations are compatible with ordering of a non-optimal substrate-binding site and restricted internal dynamics as a result of excluded volume effects making the protein less structurally 'strained'. ..
  9. Huang B, Johansson M, Byström A. An early step in wobble uridine tRNA modification requires the Elongator complex. RNA. 2005;11:424-36 pubmed
    ..Accordingly, a deletion of any ELP1-ELP6 or KTI11-KTI13 gene prevents an ochre suppressor tRNA that normally contains mcm5U from reading ochre stop codons. ..

More Information


  1. Kegel A, Sjöstrand J, Aström S. Nej1p, a cell type-specific regulator of nonhomologous end joining in yeast. Curr Biol. 2001;11:1611-7 pubmed
    ..Nej1p and Lif1(P), the yeast XRCC4 homolog, interacted in two independent assays, and Nej1p localized to the nucleus, suggesting that Nej1p may have a direct role in NHEJ. ..
  2. Chen C, Huang B, Anderson J, Byström A. Unexpected accumulation of ncm(5)U and ncm(5)S(2) (U) in a trm9 mutant suggests an additional step in the synthesis of mcm(5)U and mcm(5)S(2)U. PLoS ONE. 2011;6:e20783 pubmed publisher
    ..We propose two alternative explanations for this observation. One is that the intermediate cm(5)U is generated from ncm(5)U by a yet unknown mechanism and the other is that cm(5)U is formed before ncm(5)U and mcm(5)U. ..
  3. Esberg A, Moqtaderi Z, Fan X, Lu J, Struhl K, Byström A. Iwr1 protein is important for preinitiation complex formation by all three nuclear RNA polymerases in Saccharomyces cerevisiae. PLoS ONE. 2011;6:e20829 pubmed publisher
    ..Thus, Iwr1 plays an important role in preinitiation complex formation by all three nuclear RNA polymerases. ..
  4. Huang B, Lu J, Byström A. A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. RNA. 2008;14:2183-94 pubmed publisher
    ..Like the absence of the mcm(5) side chain, the lack of the s(2) group renders tRNA(mcm5s2UUC Glu) less sensitive to gamma-toxin, reinforcing the importance of the wobble nucleoside mcm(5)s(2)U for tRNA cleavage by gamma-toxin. ..
  5. Bjork G, Huang B, Persson O, Byström A. A conserved modified wobble nucleoside (mcm5s2U) in lysyl-tRNA is required for viability in yeast. RNA. 2007;13:1245-55 pubmed
    ..Surprisingly, overexpression of the mcm(5)s(2)U-lacking tRNA(Lys) alone was sufficient to restore viability of the double mutant. ..
  6. Rubinstein L, Ungar L, Harari Y, Babin V, Ben Aroya S, Merenyi G, et al. Telomere length kinetics assay (TELKA) sorts the telomere length maintenance (tlm) mutants into functional groups. Nucleic Acids Res. 2014;42:6314-25 pubmed publisher
    ..Thus, our telomere kinetics assay uncovers new functional groups, as well as complex genetic interactions between tlm mutants. ..
  7. Esberg A, Huang B, Johansson M, Byström A. Elevated levels of two tRNA species bypass the requirement for elongator complex in transcription and exocytosis. Mol Cell. 2006;24:139-48 pubmed
    ..Our results indicate that the physiologically relevant function of the evolutionary-conserved Elongator complex is in formation of modified nucleosides in tRNAs. ..
  8. Chereji R, Bharatula V, Elfving N, Blomberg J, Larsson M, Morozov A, et al. Mediator binds to boundaries of chromosomal interaction domains and to proteins involved in DNA looping, RNA metabolism, chromatin remodeling, and actin assembly. Nucleic Acids Res. 2017;45:8806-8821 pubmed publisher
    ..This suggests that Mediator plays a significant role in higher-order genome organization. ..