Experts and Doctors on molecular sequence data in Majadahonda, Madrid, Spain


Locale: Majadahonda, Madrid, Spain
Topic: molecular sequence data

Top Publications

  1. Trento A, Galiano M, Videla C, Carballal G, García Barreno B, Melero J, et al. Major changes in the G protein of human respiratory syncytial virus isolates introduced by a duplication of 60 nucleotides. J Gen Virol. 2003;84:3115-20 pubmed
    ..The changes reported here expand the examples of drastic genetic alterations that can be introduced into the G protein sequence of HRSV while it replicates in its natural host. ..
  2. Llorente M, Taylor I, López Viñas E, Gomez Puertas P, Calder L, García Barreno B, et al. Structural properties of the human respiratory syncytial virus P protein: evidence for an elongated homotetrameric molecule that is the smallest orthologue within the family of paramyxovirus polymerase cofactors. Proteins. 2008;72:946-58 pubmed publisher
    ..This model will be an useful tool to make rational mutations and test the role of specific amino acids in the oligomerization and functional properties of the HRSV P protein. ..
  3. Gonzalez M, Carrasco L. Viroporins. FEBS Lett. 2003;552:28-34 pubmed
    ..Future work will lengthen the list of viroporins and will provide a deeper understanding of their mechanisms of action. ..
  4. Alcalá B, Salcedo C, Arreaza L, Abad R, Enríquez R, De La Fuente L, et al. Antigenic and/or phase variation of PorA protein in non-subtypable Neisseria meningitidis strains isolated in Spain. J Med Microbiol. 2004;53:515-8 pubmed
    ..Another two presented a homopolymeric tract of eight adenine residues in the coding region, producing a DNA strand-slippage mechanism and PorA phase variation. ..
  5. Balsalobre L, Hernández Madrid A, Llull D, Martin Galiano A, Garcia E, Fenoll A, et al. Molecular characterization of disease-associated streptococci of the mitis group that are optochin susceptible. J Clin Microbiol. 2006;44:4163-71 pubmed
    ..pneumoniae by horizontal gene transfer. ..
  6. Valdezate S, Labayru C, Navarro A, Mantecón M, Ortega M, Coque T, et al. Large clonal outbreak of multidrug-resistant CC17 ST17 Enterococcus faecium containing Tn5382 in a Spanish hospital. J Antimicrob Chemother. 2009;63:17-20 pubmed publisher
    ..This study constitutes the first description of mef(E) in E. faecium. ..
  7. Valdezate S, Navarro A, Medina Pascual M, Carrasco G, Saez Nieto J. Molecular screening for rifampicin and fluoroquinolone resistance in a clinical population of Brucella melitensis. J Antimicrob Chemother. 2010;65:51-3 pubmed publisher
    ..The absence of topoisomerase II-IV mutations, however, cannot rule out fluoroquinolone resistance due to the interplay of different mechanisms. ..
  8. Alastruey Izquierdo A, Cuesta I, Houbraken J, Cuenca Estrella M, Monzon A, Rodriguez Tudela J. In vitro activity of nine antifungal agents against clinical isolates of Aspergillus calidoustus. Med Mycol. 2010;48:97-102 pubmed publisher
    ..Terbinafine and the echinocandins micafungin and anidulafungin, were the drugs most active against these species. ..
  9. Fenoll A, Mu oz R, Garc a E, de la Campa A. Molecular basis of the optochin-sensitive phenotype of pneumococcus: characterization of the genes encoding the F0 complex of the Streptococcus pneumoniae and Streptococcus oralis H(+)-ATPases. Mol Microbiol. 1994;12:587-98 pubmed
    ..pneumoniae wild-type (OptS) strains, and found a 100-fold difference between OptS and OptR strains, both in growth inhibition and in membrane ATPase resistance...

More Information


  1. Mart n Galiano A, Gorgojo B, Kunin C, de la Campa A. Mefloquine and new related compounds target the F(0) complex of the F(0)F(1) H(+)-ATPase of Streptococcus pneumoniae. Antimicrob Agents Chemother. 2002;46:1680-7 pubmed
    ..These results suggest that the transmembrane helices of the c and a subunits interact and that the mutated residues are important for the structure of the F(0) complex and proton translocation...
  2. Bello G, Casado C, Garcia S, Rodriguez C, del Romero J, Carvajal Rodriguez A, et al. Lack of temporal structure in the short term HIV-1 evolution within asymptomatic naïve patients. Virology. 2007;362:294-303 pubmed
    ..These results support the existence of distinct patterns of env evolution in untreated HIV-1-infected patients. ..
  3. de Arellano E, Alcami J, López M, Soriano V, Holguin A. Drastic decrease of transcription activity due to hypermutated long terminal repeat (LTR) region in different HIV-1 subtypes and recombinants. Antiviral Res. 2010;88:152-9 pubmed publisher
    ..G to A hypermutation at some important transcription binding-factor sites within LTR compromised the activity of the viral promoter, decreasing the in vitro viral transcription of the luciferase gene. ..
  4. Balsalobre L, Ferr ndiz M, Li ares J, Tubau F, de la Campa A. Viridans group streptococci are donors in horizontal transfer of topoisomerase IV genes to Streptococcus pneumoniae. Antimicrob Agents Chemother. 2003;47:2072-81 pubmed
    ..These results suggest that the viridans group streptococci are acting as donors in the horizontal transfer of fluoroquinolone resistance genes to S. pneumoniae...
  5. Ferrer E, Moyano E, Benitez L, Gonzalez L, Bryce D, Foster Cuevas M, et al. Cloning and characterization of Taenia saginata paramyosin cDNA. Parasitol Res. 2003;91:60-7 pubmed
    ..6% of sera from T. solium seropositive cysticercosis patients and 100% of the sera from acute cysticercosis patients. The immunodominant epitope was the carboxyl-terminal fragment of the molecule...
  6. Delgado E, Carrera C, Nebreda P, Fernandez Garcia A, Pinilla M, García V, et al. Identification of new splice sites used for generation of rev transcripts in human immunodeficiency virus type 1 subtype C primary isolates. PLoS ONE. 2012;7:e30574 pubmed publisher
    ..Usage of the newly identified splice sites is consistent with sequence features commonly found in subtype C viruses. These results show that splice site usage may differ between HIV-1 subtypes. ..
  7. Cabrerizo M, Echevarria J, Otero A, Lucas P, Trallero G. Molecular characterization of a coxsackievirus A24 variant that caused an outbreak of acute haemorrhagic conjunctivitis in Spain, 2004. J Clin Virol. 2008;43:323-7 pubmed publisher
    ..Coxsackievirus A24 variant is one of the major etiological agents involved in acute haemorrhagic conjunctivitis...
  8. Alastruey Izquierdo A, Cuenca Estrella M, Monzon A, Rodriguez Tudela J. Prevalence and susceptibility testing of new species of pseudallescheria and scedosporium in a collection of clinical mold isolates. Antimicrob Agents Chemother. 2007;51:748-51 pubmed
    ..Four Scedosporium aurantiacum isolates were detected among the panel of clinical strains. The susceptibility profile of S. aurantiacum was similar to that of Scedosporium apiospermum. ..
  9. Jado I, Escudero R, Gil H, Jiménez Alonso M, Sousa R, Garcia Perez A, et al. Molecular method for identification of Rickettsia species in clinical and environmental samples. J Clin Microbiol. 2006;44:4572-6 pubmed
    ..The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental samples. The generic approach used allowed us to identify new pathogens...
  10. Herrera Le n S, Saco M, Mart nez Silvestre A, Silveira L, Echeita A, Usera M. Molecular characterization of a new serovar of Salmonella bongori 13,22:z39:- isolated from a lizard. Res Microbiol. 2005;156:597-602 pubmed publisher
    ..We conclude that these three isolates are members of the S. bongori species representing a new serovar that will be described in the next supplement to the Kauffmann-White scheme...
  11. Ferrer E, Gonzalez L, Foster Cuevas M, Cortéz M, Dávila I, Rodriguez M, et al. Taenia solium: characterization of a small heat shock protein (Tsol-sHSP35.6) and its possible relevance to the diagnosis and pathogenesis of neurocysticercosis. Exp Parasitol. 2005;110:1-11 pubmed
    ..solium infected pigs and 84% of patients with active, or 71% of patients with inactive NCC were sero-positive. The possible relevance of Tsol-sHSP35.6 in the diagnosis and pathogenesis of NCC is discussed. ..
  12. Carrera C, Pinilla M, Pérez Alvarez L, Thomson M. Identification of unusual and novel HIV type 1 spliced transcripts generated in vivo. AIDS Res Hum Retroviruses. 2010;26:815-20 pubmed publisher
    ..These results represent the first report on the in vivo generation of diverse novel HIV-1-spliced transcripts, frequently encoding a Nef C-terminal peptide...
  13. Perez Alvarez L, Carmona R, Ocampo A, Asorey A, Miralles C, Pérez de Castro S, et al. Long-term monitoring of genotypic and phenotypic resistance to T20 in treated patients infected with HIV-1. J Med Virol. 2006;78:141-7 pubmed
  14. Casado G, Thomson M, Sierra M, Najera R. Identification of a novel HIV-1 circulating ADG intersubtype recombinant form (CRF19_cpx) in Cuba. J Acquir Immune Defic Syndr. 2005;40:532-7 pubmed
    ..These results allow us to define CRF19_cpx as a second novel CRF of African origin circulating in Cuba, to identify putative representative viruses of its parental strains, and to characterize a unique CRF18/CRF19 recombinant virus. ..
  15. Delgado E, Ríos M, Fernandez J, Pérez Alvarez L, Najera R, Thomson M. Identification of a new HIV type 1 BF intersubtype circulating recombinant form (CRF44_BF) in Chile. AIDS Res Hum Retroviruses. 2010;26:821-6 pubmed publisher
    ..Coincident breakpoints and phylogenetic clustering of the newly identified CRF44_BF with CRF12_BF, CRF17_BF, and CRF38_BF support a common origin of different CRF_BFs identified in Argentina, Uruguay, and Chile. ..
  16. Revilla A, Delgado E, Christian E, Dalrymple J, Vega Y, Carrera C, et al. Construction and phenotypic characterization of HIV type 1 functional envelope clones of subtypes G and F. AIDS Res Hum Retroviruses. 2011;27:889-901 pubmed publisher
  17. Perez Castillo A, Perez Castillo A, Saez Nieto J. Sequence of the penicillin-binding protein 2-encoding gene (penA) of Neisseria perflava/sicca. Gene. 1994;146:91-3 pubmed
    ..perflava/sicca. ..
  18. Oteo J, Hernandez J, Espasa M, Fleites A, Sáez D, Bautista V, et al. Emergence of OXA-48-producing Klebsiella pneumoniae and the novel carbapenemases OXA-244 and OXA-245 in Spain. J Antimicrob Chemother. 2013;68:317-21 pubmed publisher
    ..To describe the molecular and population-level characterization of a selected group of OXA-48-like-producing Klebsiella pneumoniae isolates collected in Spain between January 2011 and May 2012...
  19. Martinez Laso J, Herraiz M, Penaloza J, Barbolla M, Jurado M, Macedo J, et al. Promoter sequences confirm the three different evolutionary lineages described for HLA-G. Hum Immunol. 2013;74:383-8 pubmed publisher
    ..Expression and functional assays could be done considering the differences obtained in the promoter regions involving the HLA-G function (mRNA expression, isoforms). ..
  20. Lorenzo Redondo R, Borderia A, Lopez Galindez C. Dynamics of in vitro fitness recovery of HIV-1. J Virol. 2011;85:1861-70 pubmed publisher
    ..In summary, the HIV-1 in vitro fitness recovery depicts a multiphase process occurring first by generation of mutations followed by fixation of the beneficial ones, depicting a classical Darwinian process. ..
  21. Thomson M, Vinogradova A, Delgado E, Rakhmanova A, Yakovlev A, Cuevas M, et al. Molecular epidemiology of HIV-1 in St Petersburg, Russia: predominance of subtype A, former Soviet Union variant, and identification of intrasubtype subclusters. J Acquir Immune Defic Syndr. 2009;51:332-9 pubmed publisher
    ..The results of this study show a great predominance of AFSU viruses in St Petersburg and point to a few phylogenetically identifiable introductions as the origin of most current HIV-1 AFSU infections in the city. ..
  22. Gonzalez L, Ramiro R, García L, Parkhouse R, McManus D, Garate T. Genetic variability of the 18 kDa/HP6 protective antigen in Taenia saginata and Taenia asiatica: implications for vaccine development. Mol Biochem Parasitol. 2011;176:131-4 pubmed publisher
    ..solium isolates as previously described. The implications of these findings on the future development of taeniid vaccines are discussed...
  23. Ordonez D, Gómez Lozano N, Rosales L, Vilches C. Molecular characterisation of KIR2DS2*005, a fusion gene associated with a shortened KIR haplotype. Genes Immun. 2011;12:544-51 pubmed publisher
    ..2% of Caucasoids. Using a combination of two monoclonal antibodies, we also demonstrate that KIR2DS2*005 encodes a molecule expressed on the surface of natural killer- and T-lymphocytes. ..
  24. Mu oz R, Bustamante M, de la Campa A. Ser-127-to-Leu substitution in the DNA gyrase B subunit of Streptococcus pneumoniae is implicated in novobiocin resistance. J Bacteriol. 1995;177:4166-70 pubmed
    ..pneumoniae. This amino acid position is equivalent to Val-120 of Escherichia coli GyrB, a residue that lies inside the ATP-binding domain as revealed by the crystal structure of the protein...
  25. Martinez N, García Domínguez C, Domingo B, Oliva J, Zarich N, Sanchez A, et al. Sprouty2 binds Grb2 at two different proline-rich regions, and the mechanism of ERK inhibition is independent of this interaction. Cell Signal. 2007;19:2277-85 pubmed
    ..These results are evidence that the Sprouty2 mechanism of ERK inhibition is independent of Grb2 binding. ..
  26. Asenjo A, Rodr guez L, Villanueva N. Determination of phosphorylated residues from human respiratory syncytial virus P protein that are dynamically dephosphorylated by cellular phosphatases: a possible role for serine 54. J Gen Virol. 2005;86:1109-20 pubmed publisher
    ..Nonetheless, dephosphorylation at S54 could facilitate P-M protein interactions that probably occur during the egress of viral particles...
  27. Balas D, Fern ndez Moreira E, de la Campa A. Molecular characterization of the gene encoding the DNA gyrase A subunit of Streptococcus pneumoniae. J Bacteriol. 1998;180:2854-61 pubmed
    ..Primer extension analysis showed that gyrA transcription initiates 6 bp downstream of an extended -10 promoter. The possible implications of the bent DNA region as a regulatory element in the transcription of gyrA are discussed...
  28. Oliva J, Perez Sala D, Castrillo A, Martinez N, Canada F, Bosca L, et al. The cyclopentenone 15-deoxy-delta 12,14-prostaglandin J2 binds to and activates H-Ras. Proc Natl Acad Sci U S A. 2003;100:4772-7 pubmed
    ..These results describe a mechanism for the activation of the Ras signaling pathway, which results from the chemical modification of H-Ras by formation of a covalent adduct with cyclopentenone prostaglandins. ..
  29. Gonzalez L, Daniel Mwambete K, Montero E, Rosenzvit M, McManus D, Garate T, et al. Further molecular discrimination of Spanish strains of Echinococcus granulosus. Exp Parasitol. 2002;102:46-56 pubmed
    ..This observation could have important consequences for human health in Spain. Furthermore, the Eg9-PCR, Eg16-PCR, and Eg9-PCR-RFLP protocols can be used as additional methods to discriminate various E. granulosus genotypes...
  30. Olivares I, Menendez Arias L, Rodríguez Bernabé A, Martin M, Dopazo J, Lopez Galindez C. Sequence analysis of HIV-1 vif gene in Spanish isolates. Virus Genes. 1995;9:283-8 pubmed
    ..Phylogenetic analysis supports the existence of two virus lineages circulating preferentially within different risk groups. ..
  31. Perteguer M, G mez Puertas P, Ca avate C, Dagger F, G rate T, Valdivieso E. Ddi1-like protein from Leishmania major is an active aspartyl proteinase. Cell Stress Chaperones. 2013;18:171-81 pubmed publisher
    ..Our results indicate that the isolated Ddi1-like protein is a functional aspartyl proteinase in L. major, opening possibility to be considered as a potential target for novel antiparasitic drugs...
  32. Fenoll A, Munoz R, Garcia E, de la Campa A. Optochin sensitivity is encoded by the atpC gene of the Streptococcus pneumonia atp (F0F1 H(+)-ATPase) operon. Dev Biol Stand. 1995;85:287-91 pubmed
  33. Gonz lez I, Georgiou M, Alcaide F, Balas D, Li ares J, de la Campa A. Fluoroquinolone resistance mutations in the parC, parE, and gyrA genes of clinical isolates of viridans group streptococci. Antimicrob Agents Chemother. 1998;42:2792-8 pubmed
    ..pneumoniae only when mutations in parC were present. These results suggest that DNA topoisomerase IV is a primary target of ciprofloxacin in viridans group streptococci, DNA gyrase being a secondary target...
  34. Gonzalez L, Montero E, Morakote N, Puente S, Díaz de Tuesta J, Serra T, et al. Differential diagnosis of Taenia saginata and Taenia saginata asiatica taeniasis through PCR. Diagn Microbiol Infect Dis. 2004;49:183-8 pubmed
    ..saginata asiatica PCR. These new protocols have immediate potential for the specific, sensitive, and rapid identification of T. saginata asiatica and may assist in taxonomic studies...
  35. Ferrer E, Martínez Escribano J, Barderas M, Gonzalez L, Cortéz M, Dávila I, et al. Peptide epitopes of the Taenia solium antigen Ts8B2 are immunodominant in human and porcine cysticercosis. Mol Biochem Parasitol. 2009;168:168-71 pubmed publisher
  36. García Esteban C, Gil H, Rodríguez Vargas M, Gerrikagoitia X, Barandika J, Escudero R, et al. Molecular method for Bartonella species identification in clinical and environmental samples. J Clin Microbiol. 2008;46:776-9 pubmed
    ..This assay could simultaneously detect 20 different known species and other Bartonella species not described previously...
  37. Mart n Galiano A, Ferr ndiz M, de la Campa A. The promoter of the operon encoding the F0F1 ATPase of Streptococcus pneumoniae is inducible by pH. Mol Microbiol. 2001;41:1327-38 pubmed
    ..A point mutation at the -10 box of the promoter (change to TGCACT) avoided this increase, suggesting a role for this sequence in the pH-inducible regulation...
  38. Rodriguez Tudela J, Alcazar Fuoli L, Mellado E, Alastruey Izquierdo A, Monzon A, Cuenca Estrella M. Epidemiological cutoffs and cross-resistance to azole drugs in Aspergillus fumigatus. Antimicrob Agents Chemother. 2008;52:2468-72 pubmed publisher
    ..The results obtained in this study will help to develop clinical breakpoints for azole drugs and A. fumigatus. ..
  39. Perez Boto D, López Portolés J, Simon C, Valdezate S, Echeita M. Study of the molecular mechanisms involved in high-level macrolide resistance of Spanish Campylobacter jejuni and Campylobacter coli strains. J Antimicrob Chemother. 2010;65:2083-8 pubmed publisher
    ..To investigate the molecular mechanisms involved in the high-level erythromycin resistance of clinical Spanish Campylobacter jejuni and Campylobacter coli strains...
  40. Valdezate S, Miranda C, Navarro A, Freitas A, Cabrera J, Carrasco G, et al. Clonal outbreak of ST17 multidrug-resistant Enterococcus faecium harbouring an Inc18-like::Tn1546 plasmid in a haemo-oncology ward of a Spanish hospital. J Antimicrob Chemother. 2012;67:832-6 pubmed publisher
    ..To report a clonal outbreak of ST17 vancomycin-resistant Enterococcus faecium (VREfm) carrying Tn1546 (vanA) in a haemo-oncology ward of a tertiary teaching hospital in the south of Spain (January-September 2009)...
  41. Calonge E, Alonso Lobo J, Escandon C, Gonzalez N, Bermejo M, Santiago B, et al. c/EBPbeta is a major regulatory element driving transcriptional activation of the CXCL12 promoter. J Mol Biol. 2010;396:463-72 pubmed publisher
    ..Our data show that c/EBPbeta is a major regulatory element driving transcription of the CXCL12 gene in response to cytokines and cell confluence. ..