Experts and Doctors on anisakis in Madrid, Spain


Locale: Madrid, Spain
Topic: anisakis

Top Publications

  1. Valdivieso E, Perteguer M, Hurtado C, Campioli P, Rodríguez E, Saborido A, et al. ANISERP: a new serpin from the parasite Anisakis simplex. Parasit Vectors. 2015;8:399 pubmed publisher
    ..The fact that heparin does not modulate the anticoagulant activity of ANISERP might be explained by the absence in the latter of five of the six positively charged residues usually seen at the AT-III-heparin binding site. ..
  2. Daschner A, De Frutos C, Valls A, Vega F. Anisakis simplex sensitization-associated urticaria: short-lived immediate type or prolonged acute urticaria. Arch Dermatol Res. 2010;302:625-9 pubmed publisher
    ..Thus, in an allergological evaluation of urticaria, we propose a possible benefit of a distinction of the duration period at 48 h, and not 6 weeks, when differentiating acute versus chronic urticaria. ..
  3. Lopez Serrano M, Gomez A, Daschner A, Moreno Ancillo A, de Parga J, Caballero M, et al. Gastroallergic anisakiasis: findings in 22 patients. J Gastroenterol Hepatol. 2000;15:503-6 pubmed
    ..The purpose of this study was to analyse the clinical findings in 22 patients with gastroallergic anisakiasis...
  4. Rojas Perez Ezquerra P, Noguerado Mellado B, Morales Cabeza C, Zambrano Ibarra G, Datino Romaniega T. Atrial Fibrillation in Anaphylaxis. Am J Med. 2017;130:1114-1116 pubmed publisher
  5. Daschner A, Cuellar C, Sánchez Pastor S, Pascual C, Martín Esteban M. Gastro-allergic anisakiasis as a consequence of simultaneous primary and secondary immune response. Parasite Immunol. 2002;24:243-51 pubmed
  6. López Sáez M, Zubeldia J, Caloto M, Olalde S, Pelta R, Rubio M, et al. Is Anisakis simplex responsible for chronic urticaria?. Allergy Asthma Proc. 2003;24:339-45 pubmed
    ..We have not found any causal relationship between the presence of specific IgE to A. simplex and CU. The clinical importance of this finding in this disease is still undetermined. ..
  7. Baeza M, Rodriguez A, Matheu V, Rubio M, Tornero P, De Barrio M, et al. Characterization of allergens secreted by Anisakis simplex parasite: clinical relevance in comparison with somatic allergens. Clin Exp Allergy. 2004;34:296-302 pubmed
    ..Diagnostic methods for the study of allergic reactions to Anisakis simplex (A.s.) based on whole-body extracts of the larva are clearly insufficient...
  8. Alonso Gómez A, Moreno Ancillo A, López Serrano M, Suarez de Parga J, Daschner A, Caballero M, et al. Anisakis simplex only provokes allergic symptoms when the worm parasitises the gastrointestinal tract. Parasitol Res. 2004;93:378-84 pubmed
    ..Allergic symptoms are the most frequent manifestation of A. simplex parasitism. We could not find any patient allergic to the thermostable proteins of parasite...
  9. Caballero M, Umpierrez A, Pérez Piñar T, Moneo I, de Burgos C, Asturias J, et al. Anisakis simplex recombinant allergens increase diagnosis specificity preserving high sensitivity. Int Arch Allergy Immunol. 2012;158:232-40 pubmed publisher
    ..simplex diagnosis while keeping the highest sensitivity. A. simplex recombinant allergens should be included with A. simplex allergy diagnostic tests to improve their specificity. ..

More Information


  1. Rodriguez Mahillo A, Gonzalez Munoz M, Gomez Aguado F, Rodriguez Perez R, Corcuera M, Caballero M, et al. Cloning and characterisation of the Anisakis simplex allergen Ani s 4 as a cysteine-protease inhibitor. Int J Parasitol. 2007;37:907-17 pubmed
    ..The resulting rAni s 4 retains all allergenic properties of the natural allergen, and can therefore be used in immunodiagnosis of human anisakiasis...
  2. Caballero M, Umpierrez A, Moneo I, Rodriguez Perez R. Ani s 10, a new Anisakis simplex allergen: cloning and heterologous expression. Parasitol Int. 2011;60:209-12 pubmed publisher
    ..A total of 30 of 77 Anisakis allergic patients (39%) were positive both to rAni s 10 and natural Ani s 10 by immunoblotting. The new allergen could be useful in a component-resolved diagnosis system for Anisakis allergy...
  3. Daschner A, Rodero M, De Frutos C, Valls A, Cuellar C. Chronic urticaria is associated with a differential helminth-arthropod-related atopy phenotype. J Dermatol. 2010;37:780-5 pubmed publisher
    ..03), whereas no difference could be stated for PMD sensitization. Compared to RCBA patients, both CU/As+ and CU/As- patients have a higher clinically relevant sensitization rate against HDM, thus displaying a differential atopy phenotype...
  4. Rodriguez E, Anadón A, García Bodas E, Romaris F, Iglesias R, Garate T, et al. Novel sequences and epitopes of diagnostic value derived from the Anisakis simplex Ani s 7 major allergen. Allergy. 2008;63:219-25 pubmed publisher
    ..The aim of this study was to clone and characterize the Ani s 7 major allergen, and to obtain a recombinant fragment suitable for serodiagnosis...
  5. Garcia Hernandez P, Rodero M, Cuellar C. Study of the effect of Anisakis simplex larval products on the early and late components in the classical complement pathway. J Parasitol. 2009;95:240-1 pubmed publisher
    ..Therefore, CE and ES products from A. simplex interact with C3 and C2 complement proteins, which are early components of the complement system, but not with the late complement components. ..
  6. Perteguer M, Chivato T, Montoro A, Cuellar C, Mateos J, Laguna R. Specific and total IgE in patients with recurrent, acute urticaria caused by Anisakis simplex. Ann Trop Med Parasitol. 2000;94:259-68 pubmed
    ..Most (16) of the patients no longer had any urticaria and the others reported significant reductions in the intensity and frequency of their symptoms...
  7. Rodríguez Mahillo A, Gonzalez Munoz M, de las Heras C, Tejada M, Moneo I. Quantification of Anisakis simplex allergens in fresh, long-term frozen, and cooked fish muscle. Foodborne Pathog Dis. 2010;7:967-73 pubmed publisher
    ..Basophil activation tests have proven the capability of the A. simplex-positive fish extracts to induce allergic symptoms. ..
  8. Zuloaga J, Rodríguez Bobada C, Corcuera M, Gomez Aguado F, Gonzalez P, Rodriguez Perez R, et al. A rat model of intragastric infection with Anisakis spp. live larvae: histopathological study. Parasitol Res. 2013;112:2409-11 pubmed publisher
    ..The model of infection described is valid to study the behavior of the larvae inside the host body, the histopathological changes at the invasion site, and the effects of the repeated infections by ingestion of live larvae...
  9. Caballero M, Asero R, Antonicelli L, Kamberi E, Colangelo C, Fazii P, et al. Anisakis allergy component-resolved diagnosis: clinical and immunologic differences between patients from Italy and Spain. Int Arch Allergy Immunol. 2013;162:39-44 pubmed publisher
    ..The results were compared with those obtained previously in a similar Spanish population...
  10. Daschner A, Cuellar C, Valls A. Towards a differential definition of atopy: Anisakis simplex and the relationship between parasites and arthropods in respiratory allergy. Parasite Immunol. 2008;30:417-24 pubmed publisher
    ..We propose a differential definition of atopy, with a special emphasis on arthropod related sensitization. ..
  11. Rodriguez Mahillo A, Gonzalez Munoz M, Moneo I. Identification and allergenic characterisation of a new isoform of the A. simplex allergen Ani s 4. Mol Biochem Parasitol. 2008;160:152-6 pubmed publisher
    ..Both isoforms of Ani s 4 showed different capacities to bind IgE from sensitised patients and different potencies in the basophil activation test. ..
  12. Puente P, Anadón A, Rodero M, Romaris F, Ubeira F, Cuellar C. Anisakis simplex: the high prevalence in Madrid (Spain) and its relation with fish consumption. Exp Parasitol. 2008;118:271-4 pubmed
    ..These results are discussed in relation to the much lower prevalence observed in Galicia (N.W. Spain)...
  13. Garcia Hernandez P, Rodero M, Cuellar C. Anisakis simplex: the activity of larval products on the complement system. Exp Parasitol. 2007;115:1-8 pubmed
    ..Heating the crude parasite extract led to a notable loss of haemolysis inhibition activity, and the addition of PMSF (a serine protease inhibitor) also cause variation in the activity of the crude extract...
  14. Arias Diaz J, Zuloaga J, Vara E, Balibrea J, Balibrea J. Efficacy of albendazole against Anisakis simplex larvae in vitro. Dig Liver Dis. 2006;38:24-6 pubmed
    ..Albendazole is effective in killing A. simplex larvae at different pH in vitro, suggesting that this molecule could be useful in treating clinical manifestations of human anisakidosis. ..
  15. Daschner A, Pascual C. Anisakis simplex: sensitization and clinical allergy. Curr Opin Allergy Clin Immunol. 2005;5:281-5 pubmed
    ..This article reviews recent knowledge of allergic disorders ascribed to A. simplex contact or parasitism...
  16. Perteguer M, Aguila C, Fenoy S, Guillen J, Cuellar C. Cross-reactivity induced by Anisakis simplex and Toxocara canis in mice. J Helminthol. 2003;77:331-4 pubmed
    ..canis eggs were compared with those obtained by testing sera from mice infected with one A. simplex L3, an increase in cross-reactions was observed using the C57BL/10 strain...
  17. Gutierrez R, Cuellar C. Immunoglobulins anti-Anisakis simplex in patients with gastrointestinal diseases. J Helminthol. 2002;76:131-6 pubmed
    ..Specific IgA is associated with a higher activity index of Crohn's disease. Specific antibodies were observed against A. simplex in patients with appendicitis and gastrointestinal cancer, indicating a higher rate of positivity for IgA. ..
  18. Cuellar C, Perteguer M, Rodero M. Presence of IL-4-like molecules in larval excretory-secretory products and crude extracts from Anisakis simplex. Scand J Immunol. 2001;53:483-8 pubmed
  19. Caballero M, Moneo I, Gomez Aguado F, Corcuera M, Casado I, Rodriguez Perez R. Isolation of Ani s 5, an excretory-secretory and highly heat-resistant allergen useful for the diagnosis of Anisakis larvae sensitization. Parasitol Res. 2008;103:1231-3 pubmed publisher
  20. Rodriguez Perez R, Moneo I, Rodriguez Mahillo A, Caballero M. Cloning and expression of Ani s 9, a new Anisakis simplex allergen. Mol Biochem Parasitol. 2008;159:92-7 pubmed publisher
    ..8%) were positive to rAni s 9 and natural Ani s 9 by immunodetection. In conclusion, Ani s 9 is a new allergen in Anisakis allergy and it has been cloned and successfully expressed in E. coli...
  21. Jurado Palomo J, Lopez Serrano M, Moneo I. Multiple acute parasitization by Anisakis simplex. J Investig Allergol Clin Immunol. 2010;20:437-41 pubmed
    ..However, the patient only developed an immune response with specific immunoglobulin E and eosinophilia peaking at day 18 and decreasing during the 17-month follow-up. Only eosinophilia reached normal limits. ..
  22. Cuellar C, Daschner A, Valls A, De Frutos C, Fernández Fígares V, Anadón A, et al. Ani s 1 and Ani s 7 recombinant allergens are able to differentiate distinct Anisakis simplex-associated allergic clinical disorders. Arch Dermatol Res. 2012;304:283-8 pubmed publisher
    ..simplex-associated allergic disorders. The IgE responses to Ani s 1 are mainly associated with GAA, while this molecule cannot be considered a major allergen in CU+ patients...
  23. González Fernández J, Rivas L, Luque Ortega J, Núñez Ramírez R, Campioli P, Garate T, et al. Recombinant vs native Anisakis haemoglobin (Ani s 13): Its appraisal as a new gold standard for the diagnosis of allergy. Exp Parasitol. 2017;181:119-129 pubmed publisher
    ..None of the non-Anisakis sensitised patients were positive. Combined use of purified native form of Ani s 13 with current gold standards would improve the sensitivity and specificity for diagnosing anisakiasis. ..