Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae proteins in Barcelona, Catalonia, Spain
Locale: Barcelona, Catalonia, Spain
Topic: saccharomyces cerevisiae proteins
- Neurohr G, Naegeli A, Titos I, Theler D, Greber B, Diez J, et al. A midzone-based ruler adjusts chromosome compaction to anaphase spindle length. Science. 2011;332:465-8 pubmed publisher..Thus, the anaphase spindle may function as a ruler to adapt the condensation of chromatids, promoting their segregation regardless of chromosome or spindle length. ..
- Macias S, Bragulat M, Tardiff D, Vilardell J. L30 binds the nascent RPL30 transcript to repress U2 snRNP recruitment. Mol Cell. 2008;30:732-42 pubmed publisher..Collectively, our data suggest that L30 represses a spliceosomal rearrangement required for U2 snRNP association with the transcript. ..
- de Nadal E, Posas F. Multilayered control of gene expression by stress-activated protein kinases. EMBO J. 2010;29:4-13 pubmed publisher..Thus, elucidating the control of gene expression by the Hog1 SAPK should help to understand how eukaryotic cells implement a massive and rapid change on their transcriptional capacity in response to adverse conditions. ..
- Meyer M, Vilardell J. The quest for a message: budding yeast, a model organism to study the control of pre-mRNA splicing. Brief Funct Genomic Proteomic. 2009;8:60-7 pubmed publisher..This should provide a valuable system to approach the complex problem of the role of regulated splicing in genomic expression. ..
- Rodriguez Hernandez C, Guinovart J, Murguia J. Anti-diabetic and anti-obesity agent sodium tungstate enhances GCN pathway activation through Glc7p inhibition. FEBS Lett. 2012;586:270-6 pubmed publisher..Therefore tungstate alleviates nutrient stress in yeast by in vivo inhibition of Glc7p. These data uncover a potential role for tungstate in the treatment of PP1 and GCN related diseases. ..
- Marquina M, González A, Barreto L, Gelis S, Munoz I, Ruiz A, et al. Modulation of yeast alkaline cation tolerance by Ypi1 requires calcineurin. Genetics. 2012;190:1355-64 pubmed publisher..These results point to a complex role of Ypi1 on the regulation of cation homeostasis, largely mediated by the calcineurin phosphatase. ..
- Serrano R, Ruiz A, Bernal D, Chambers J, Arino J. The transcriptional response to alkaline pH in Saccharomyces cerevisiae: evidence for calcium-mediated signalling. Mol Microbiol. 2002;46:1319-33 pubmed..These results demonstrate that the transcriptional response to alkaline pH involves different signalling mechanisms, and that calcium signalling is a relevant component of this response. ..
- Ruiz A, Yenush L, Arino J. Regulation of ENA1 Na(+)-ATPase gene expression by the Ppz1 protein phosphatase is mediated by the calcineurin pathway. Eukaryot Cell. 2003;2:937-48 pubmed..In conclusion, we have identified a novel role for calcineurin, but not alkalinization, in the control of ENA1 expression in ppz1 mutants. ..
- Hjelmqvist L, Tuson M, Marfany G, Herrero E, Balcells S, Gonzalez Duarte R. ORMDL proteins are a conserved new family of endoplasmic reticulum membrane proteins. Genome Biol. 2002;3:RESEARCH0027 pubmed..Subcellular localization and the response of yeast mutants to specific agents point to the involvement of ORMDL in protein folding in the ER. ..
- de Nadal E, Calero F, Ramos J, Arino J. Biochemical and genetic analyses of the role of yeast casein kinase 2 in salt tolerance. J Bacteriol. 1999;181:6456-62 pubmed..The possible causes for the salt sensitivity phenotype of casein kinase mutants are discussed in the light of these findings. ..
- Munoz I, Ruiz A, Marquina M, Barceló A, Albert A, Arino J. Functional characterization of the yeast Ppz1 phosphatase inhibitory subunit Hal3: a mutagenesis study. J Biol Chem. 2004;279:42619-27 pubmed..In contrast, the mutation of His378 did not. This finding suggests that Hal3 may have both Ppz1-dependent and independent functions involving different structural elements. ..
- de Nadal E, Clotet J, Posas F, Serrano R, Gomez N, Arino J. The yeast halotolerance determinant Hal3p is an inhibitory subunit of the Ppz1p Ser/Thr protein phosphatase. Proc Natl Acad Sci U S A. 1998;95:7357-62 pubmed..Therefore, Hal3p might modulate diverse physiological functions of the Ppz1 phosphatase, such as salt stress tolerance and cell cycle progression, by acting as a inhibitory subunit. ..
- Galao R, Chari A, Alves Rodrigues I, Lobão D, Mas A, Kambach C, et al. LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication. RNA. 2010;16:817-27 pubmed publisher..Our results provide the first evidence that LSm1-7 complexes interact directly with viral RNA genomes and open new perspectives in the understanding of LSm1-7 functions. ..
- Duran J, Anjard C, Stefan C, Loomis W, Malhotra V. Unconventional secretion of Acb1 is mediated by autophagosomes. J Cell Biol. 2010;188:527-36 pubmed publisher..We propose that these autophagosome intermediates fuse with recycling endosomes instead to form multivesicular body carriers that then fuse with the plasma membrane to release cargo. ..
- Sole C, Nadal Ribelles M, Kraft C, Peter M, Posas F, de Nadal E. Control of Ubp3 ubiquitin protease activity by the Hog1 SAPK modulates transcription upon osmostress. EMBO J. 2011;30:3274-84 pubmed publisher..Furthermore, Ubp3 is recruited to osmoresponsive genes to modulate transcriptional initiation as well as elongation. Therefore, Ubp3 activity responds to external stimuli and is required for transcriptional activation upon osmostress. ..
- Ruiz A, Munoz I, Serrano R, González A, Simon E, Arino J. Functional characterization of the Saccharomyces cerevisiae VHS3 gene: a regulatory subunit of the Ppz1 protein phosphatase with novel, phosphatase-unrelated functions. J Biol Chem. 2004;279:34421-30 pubmed..These results indicate that, besides its role as Ppz1 inhibitory subunit, Vhs3 (and probably Hal3) might have important Ppz-independent functions. ..
- González A, Ruiz A, Casamayor A, Arino J. Normal function of the yeast TOR pathway requires the type 2C protein phosphatase Ptc1. Mol Cell Biol. 2009;29:2876-88 pubmed publisher..All of these results provide the first evidence showing that a type 2C protein phosphatase is required for the normal functioning of the TOR pathway. ..
- Hernández Ortega S, Bru S, Ricco N, Ramirez S, Casals N, Jimenez J, et al. Defective in mitotic arrest 1 (Dma1) ubiquitin ligase controls G1 cyclin degradation. J Biol Chem. 2013;288:4704-14 pubmed publisher..This new previously unknown mechanism for G(1) cyclin degradation that we report here could help elucidate the specific roles of the redundant CDK-cyclin complexes in G(1). ..
- Salceda J, Fernandez X, Roca J. Topoisomerase II, not topoisomerase I, is the proficient relaxase of nucleosomal DNA. EMBO J. 2006;25:2575-83 pubmed..We conclude that topoisomerase II is the main modulator of DNA topology in chromatin fibers. The nonessential topoisomerase I then assists DNA relaxation where chromatin structure impairs DNA juxtaposition but allows twist diffusion. ..
- Sutrias Grau M, Bianchi M, Bernues J. High mobility group protein 1 interacts specifically with the core domain of human TATA box-binding protein and interferes with transcription factor IIB within the pre-initiation complex. J Biol Chem. 1999;274:1628-34 pubmed..These results suggest a general involvement of HMG1 at the early stages of polymerase II transcription that may result in subtle activation or repression of individual genes. ..
- Cortes A, Huertas D, Fanti L, Pimpinelli S, Marsellach F, Pina B, et al. DDP1, a single-stranded nucleic acid-binding protein of Drosophila, associates with pericentric heterochromatin and is functionally homologous to the yeast Scp160p, which is involved in the control of cell ploidy. EMBO J. 1999;18:3820-33 pubmed..Expression of DDP1 complements a Deltascp160 deletion in yeast. These results are discussed in view of the possible contribution of DNA structure to the structural organization of pericentric heterochromatin. ..
- Nadal Ribelles M, Sol C, Xu Z, Steinmetz L, de Nadal E, Posas F. Control of Cdc28 CDK1 by a stress-induced lncRNA. Mol Cell. 2014;53:549-61 pubmed publisher..The increase in the levels of Cdc28 results in cells able to reenter the cell cycle more efficiently after stress. This may represent a general mechanism to prime expression of genes needed after stresses are alleviated...
- Calam E, GonzÃ¡lez Roca E, FernÃ¡ndez M, Dequin S, ParÃ©s X, Virgili A, et al. Enantioselective Synthesis of Vicinal (R,R)-Diols by Saccharomyces cerevisiae Butanediol Dehydrogenase. Appl Environ Microbiol. 2016;82:1706-1721 pubmed publisher..In conclusion, Bdh1p is an enzyme with biotechnological interest that can be used to synthesize chiral building blocks. A scheme of the favored pathway with the corresponding intermediates is proposed for the Bdh1p reaction. ..
- Bragulat M, Meyer M, Macias S, Camats M, Labrador M, Vilardell J. RPL30 regulation of splicing reveals distinct roles for Cbp80 in U1 and U2 snRNP cotranscriptional recruitment. RNA. 2010;16:2033-41 pubmed publisher..These results reveal a novel function for CBC in splicing and imply that these molecular events can be the target of a splicing regulator. ..
- Bonet R, Ramirez Espain X, Macias M. Solution structure of the yeast URN1 splicing factor FF domain: comparative analysis of charge distributions in FF domain structures-FFs and SURPs, two domains with a similar fold. Proteins. 2008;73:1001-9 pubmed publisher..This cavity is also present in at least CA150FF1 and FF2 structures. Thus, as with WW domains, the FF fold seems to have developed binding-site variations to accommodate an abundant and variable set of ligands. ..
- Molina Arcas M, Bellosillo B, Casado F, Montserrat E, Gil J, Colomer D, et al. Fludarabine uptake mechanisms in B-cell chronic lymphocytic leukemia. Blood. 2003;101:2328-34 pubmed
- Ruiz Roig C, Viéitez C, Posas F, de Nadal E. The Rpd3L HDAC complex is essential for the heat stress response in yeast. Mol Microbiol. 2010;76:1049-62 pubmed publisher..Finally, we found that the large, but not the small Rpd3 complex regulated cell adaptation in response to heat stress. ..
- Joshi R, Pina B, Roca J. Topoisomerase II is required for the production of long Pol II gene transcripts in yeast. Nucleic Acids Res. 2012;40:7907-15 pubmed publisher..Apparently, only topo II relaxes efficiently the (+) DNA supercoils that stall the advancement of Pol II in long genes. A mechanistic model is proposed. ..
- Yaakov G, Duch A, Garcia Rubio M, Clotet J, Jimenez J, Aguilera A, et al. The stress-activated protein kinase Hog1 mediates S phase delay in response to osmostress. Mol Biol Cell. 2009;20:3572-82 pubmed publisher..The two mechanisms of Hog1 action lead to delayed firing of origins and prolonged replication, respectively. The Hog1-dependent delay of replication could be important to allow Hog1 to induce gene expression before replication. ..
- Menoyo S, Ricco N, Bru S, Hernandez Ortega S, Escote X, Aldea M, et al. Phosphate-activated cyclin-dependent kinase stabilizes G1 cyclin to trigger cell cycle entry. Mol Cell Biol. 2013;33:1273-84 pubmed publisher..Taken together, our data indicate that Cln3 is a molecular target of the Pho85 kinase that is required to modulate cell cycle entry in response to environmental changes in nutrient availability. ..
- Mas G, de Nadal E, Dechant R, Rodríguez de la Concepción M, Logie C, Jimeno González S, et al. Recruitment of a chromatin remodelling complex by the Hog1 MAP kinase to stress genes. EMBO J. 2009;28:326-36 pubmed publisher..Our results suggest that the selective targeting of the RSC complex by Hog1 provides the necessary mechanistic basis for this event. ..
- Isasa M, Katz E, Kim W, Yugo V, González S, Kirkpatrick D, et al. Monoubiquitination of RPN10 regulates substrate recruitment to the proteasome. Mol Cell. 2010;38:733-45 pubmed publisher..Our results reveal an unanticipated link between monoubiquitination signal and regulation of proteasome function. ..
- Sabat M, Navarro F, Mir E, Campoy S, Mirelis B, Barb J, et al. Novel complex sul1-type integron in Escherichia coli carrying bla(CTX-M-9). Antimicrob Agents Chemother. 2002;46:2656-61 pubmed..Furthermore, a new insertion sequence, IS3000, has been found in In60. PCR analysis indicates that integron In60 is present in 33 of 34 nonclonal enterobacterial isolates carrying the putative beta-lactamase CTX-M-9...
- Ruiz A, del Carmen Ruiz M, Sánchez Garrido M, Arino J, Ramos J. The Ppz protein phosphatases regulate Trk-independent potassium influx in yeast. FEBS Lett. 2004;578:58-62 pubmed
- Ferrer Dalmau J, González A, Platara M, Navarrete C, Martinez J, Barreto L, et al. Ref2, a regulatory subunit of the yeast protein phosphatase 1, is a novel component of cation homoeostasis. Biochem J. 2010;426:355-64 pubmed publisher..Remarkably, the evidence collected indicates that the role of Ref2 in cation homoeostasis may be unrelated to its previously identified function in the formation of mRNA via the APT (for associated with Pta1) complex. ..
- Alves Rodrigues I, Mas A, Diez J. Xenopus Xp54 and human RCK/p54 helicases functionally replace yeast Dhh1p in brome mosaic virus RNA replication. J Virol. 2007;81:4378-80 pubmed..This functional conservation, together with the common replication strategies of positive-stranded RNA viruses, suggests that RCK/p54 may also play a role in the replication of positive-stranded RNA viruses that infect humans. ..
- Fernandez M, Porte S, Crosas E, Barbera N, Farres J, Biosca J, et al. Human and yeast zeta-crystallins bind AU-rich elements in RNA. Cell Mol Life Sci. 2007;64:1419-27 pubmed..This supports a role for zeta-crystallins as trans-acting factors that could regulate the turnover of certain mRNAs. ..
- Ruiz A, Arino J. Function and regulation of the Saccharomyces cerevisiae ENA sodium ATPase system. Eukaryot Cell. 2007;6:2175-83 pubmed
- Mosca R, Pons C, Fernandez Recio J, Aloy P. Pushing structural information into the yeast interactome by high-throughput protein docking experiments. PLoS Comput Biol. 2009;5:e1000490 pubmed publisher..All docking results are available at http://gatealoy.pcb.ub.es/HT_docking/. ..
- Marsellach F, Huertas D, Azorín F. The multi-KH domain protein of Saccharomyces cerevisiae Scp160p contributes to the regulation of telomeric silencing. J Biol Chem. 2006;281:18227-35 pubmed..These results are discussed in the context of a model in which Scp160p contributes to silencing by helping telomere clustering. ..
- Ruiz A, González A, García Salcedo R, Ramos J, Arino J. Role of protein phosphatases 2C on tolerance to lithium toxicity in the yeast Saccharomyces cerevisiae. Mol Microbiol. 2006;62:263-77 pubmed..These results indicate that Ptc1p modulates the function of Ena1p by regulating the Hal3/Ppz1,2 pathway. In conclusion, overexpression of PTC3 and lack of PTC1 affect lithium tolerance in yeast, although through different mechanisms. ..
- Clotet J, Gari E, Aldea M, Arino J. The yeast ser/thr phosphatases sit4 and ppz1 play opposite roles in regulation of the cell cycle. Mol Cell Biol. 1999;19:2408-15 pubmed
- Pagani A, Villarreal L, Capdevila M, Atrian S. The Saccharomyces cerevisiae Crs5 Metallothionein metal-binding abilities and its role in the response to zinc overload. Mol Microbiol. 2007;63:256-69 pubmed
- Tio L, Pagani M, Atrian S. Drosophila proteins interacting with metallothioneins: a metal-dependent recognition. Proteomics. 2009;9:2568-77 pubmed publisher..Furthermore, new perspectives to investigate the often hypothesized contribution of MTs to the redox physiological networks are open. ..
- Curwin A, von Blume J, Malhotra V. Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast. Mol Biol Cell. 2012;23:2327-38 pubmed publisher..These findings highlight the conserved role of cofilin and SPCA1/Pmr1 in sorting of the soluble secretory proteins at the TGN/late Golgi membranes in eukaryotes. ..
- Thomson T, Lozano J, Loukili N, Carrio R, Serras F, Cormand B, et al. Fusion of the human gene for the polyubiquitination coeffector UEV1 with Kua, a newly identified gene. Genome Res. 2000;10:1743-56 pubmed..Therefore, the addition of a Kua domain to UEV in the fused Kua-UEV protein confers new biological properties to this regulator of variant polyubiquitination. ..
- Lumbreras V, Alba M, Kleinow T, Koncz C, Pages M. Domain fusion between SNF1-related kinase subunits during plant evolution. EMBO Rep. 2001;2:55-60 pubmed..Our results suggest that evolution of AKINbetagamma reflects a unique function of SNF1-related protein kinases in plant glucose and stress signalling. ..
- Simon E, Clotet J, Calero F, Ramos J, Arino J. A screening for high copy suppressors of the sit4 hal3 synthetically lethal phenotype reveals a role for the yeast Nha1 antiporter in cell cycle regulation. J Biol Chem. 2001;276:29740-7 pubmed
- Clotet J, Posas F, de Nadal E, Arino J. The NH2-terminal extension of protein phosphatase PPZ1 has an essential functional role. J Biol Chem. 1996;271:26349-55 pubmed..These results indicates that the NH2-terminal half of the protein contains structural determinants that are specific for certain functions and that the phosphatase activity is required but not sufficient for full PPZ1 function. ..
- Casado C, Yenush L, Melero C, Ruiz M, Serrano R, Perez Valle J, et al. Regulation of Trk-dependent potassium transport by the calcineurin pathway involves the Hal5 kinase. FEBS Lett. 2010;584:2415-20 pubmed publisher..This suggests that the control exerted by calcineurin on Hal5 expression may be biologically relevant for Trk1 regulation. ..
- Crosas E, Porté S, Moeini A, Farrés J, Biosca J, Parés X, et al. Novel alkenal/one reductase activity of yeast NADPH:quinone reductase Zta1p. Prospect of the functional role for the ?-crystallin family. Chem Biol Interact. 2011;191:32-7 pubmed publisher..The role of Zta1p in the yeast adaptation to some stress types and the general functional significance of ?-crystallins are discussed. ..
- Arino J. Integrative responses to high pH stress in S. cerevisiae. OMICS. 2010;14:517-23 pubmed publisher..In this review we will examine these responses, their possible interactions, and the role that they play in tolerance to high pH stress. ..
- Navarro Sastre A, Tort F, Stehling O, Uzarska M, Arranz J, Del Toro M, et al. A fatal mitochondrial disease is associated with defective NFU1 function in the maturation of a subset of mitochondrial Fe-S proteins. Am J Hum Genet. 2011;89:656-67 pubmed publisher..Importantly, yeast Nfu1 protein carrying the individuals' missense mutation was functionally impaired. We conclude that NFU1 functions as a late-acting maturation factor for a subset of mitochondrial Fe-S proteins. ..
- Schluter A, Fourcade S, Domènech Estévez E, Gabaldón T, Huerta Cepas J, Berthommier G, et al. PeroxisomeDB: a database for the peroxisomal proteome, functional genomics and disease. Nucleic Acids Res. 2007;35:D815-22 pubmed..PeroxisomeDB should contribute to the systematic characterization of the peroxisomal proteome and facilitate system biology approaches on the organelle...
- Forés O, Arró M, Pahissa A, Ferrero S, Germann M, Stukey J, et al. Arabidopsis thaliana expresses two functional isoforms of Arvp, a protein involved in the regulation of cellular lipid homeostasis. Biochim Biophys Acta. 2006;1761:725-35 pubmed..The results of this study support the notion that plants, yeast and mammals share common molecular mechanisms regulating intracellular lipid homeostasis. ..
- Grötsch H, Giblin J, Idrissi F, Fernández Golbano I, Collette J, Newpher T, et al. Calmodulin dissociation regulates Myo5 recruitment and function at endocytic sites. EMBO J. 2010;29:2899-914 pubmed publisher..Concomitantly, calmodulin dissociation triggered Myo5 binding to Vrp1, extended the myosin-I lifespan at endocytic sites and activated Myo5-induced actin polymerization. ..