Genomes and Genes
Experts and Doctors on saccharomyces cerevisiae in Barcelona, Catalonia, Spain
Locale: Barcelona, Catalonia, Spain
Topic: saccharomyces cerevisiae
- Flores O, Deniz O, Soler Lopez M, Orozco M. Fuzziness and noise in nucleosomal architecture. Nucleic Acids Res. 2014;42:4934-46 pubmed publisher..Furthermore, we present a systematic approach that may guide the standardization of MNase-Seq experiments in order to generate reproducible genome-wide nucleosome patterns. ..
- Perapoch J, Planes A, Querol A, Lopez V, Martínez Bendayán I, Tormo R, et al. Fungemia with Saccharomyces cerevisiae in two newborns, only one of whom had been treated with ultra-levura. Eur J Clin Microbiol Infect Dis. 2000;19:468-70 pubmed..This is the first report of infection with Saccharomyces boulardii (Saccharomyces cerevisiae) in a patient who was not being treated with the agent. ..
- Bragulat M, Meyer M, Macias S, Camats M, Labrador M, Vilardell J. RPL30 regulation of splicing reveals distinct roles for Cbp80 in U1 and U2 snRNP cotranscriptional recruitment. RNA. 2010;16:2033-41 pubmed publisher..These results reveal a novel function for CBC in splicing and imply that these molecular events can be the target of a splicing regulator. ..
- Bru S, Samper MartÃn B, Quandt E, HernÃ¡ndez Ortega S, MartÃnez LaÃnez J, GarÃ E, et al. Polyphosphate is a key factor for cell survival after DNA damage in eukaryotic cells. DNA Repair (Amst). 2017;57:171-178 pubmed publisher..In conclusion, we present polyP as a molecule involved in resistance to DNA damage and suggest that polyP may be a putative target for new approaches in cancer treatment or prevention. ..
- Serra M, Gauthier L, Fabrias G, Buist P. Delta11 desaturases of Trichoplusia ni and Spodoptera littoralis exhibit dual catalytic behaviour. Insect Biochem Mol Biol. 2006;36:822-5 pubmed..induced) and the presence or absence of a b5-fusion domain. Our findings suggest that in future, a search for hydroxylated products should be included in functional analyses of insect desaturase genes. ..
- González A, Larroy C, Biosca J, Arino J. Use of the TRP1 auxotrophic marker for gene disruption and phenotypic analysis in yeast: a note of warning. FEMS Yeast Res. 2008;8:2-5 pubmed..Therefore, its use in the past should be revisited and utilization of this marker should be avoided in future analyses. ..
- Serrano P, Casas J, Llebaria A, Zucco M, Emeric G, Delgado A. Parallel synthesis and yeast growth inhibition screening of succinamic acid libraries. J Comb Chem. 2007;9:635-43 pubmed..Results on growth inhibition of the yeast Saccharomyces cerevisiae in the presence of selected library members are also reported as a preliminary evaluation of the antifungal activity...
- Raich L, Borodkin V, Fang W, Castro López J, van Aalten D, Hurtado Guerrero R, et al. A Trapped Covalent Intermediate of a Glycoside Hydrolase on the Pathway to Transglycosylation. Insights from Experiments and Quantum Mechanics/Molecular Mechanics Simulations. J Am Chem Soc. 2016;138:3325-32 pubmed publisher..Our results suggest that changes in the interactions involving the 2-position could have an impact on the transglycosylation activity of several GHs. ..
- Garcia Reyero N, Grau E, Castillo M, Lopez De Alda M, Barcelo D, Pina B. Monitoring of endocrine disruptors in surface waters by the yeast recombinant assay. Environ Toxicol Chem. 2001;20:1152-8 pubmed
- Pagani A, Villarreal L, Capdevila M, Atrian S. The Saccharomyces cerevisiae Crs5 Metallothionein metal-binding abilities and its role in the response to zinc overload. Mol Microbiol. 2007;63:256-69 pubmed
- Ferrer Dalmau J, González A, Platara M, Navarrete C, Martinez J, Barreto L, et al. Ref2, a regulatory subunit of the yeast protein phosphatase 1, is a novel component of cation homoeostasis. Biochem J. 2010;426:355-64 pubmed publisher..Remarkably, the evidence collected indicates that the role of Ref2 in cation homoeostasis may be unrelated to its previously identified function in the formation of mRNA via the APT (for associated with Pta1) complex. ..
- Idrissi F, Blasco A, Espinal A, Geli M. Ultrastructural dynamics of proteins involved in endocytic budding. Proc Natl Acad Sci U S A. 2012;109:E2587-94 pubmed..Altogether, this work provides major insights into the molecular mechanisms driving membrane deformation in a cellular context. ..
- Trigueros S, Roca J. Circular minichromosomes become highly recombinogenic in topoisomerase-deficient yeast cells. J Biol Chem. 2001;276:2243-8 pubmed..Yeast minichromosome multimerization provides an appropriate system in which to study mechanistic aspects of DNA recombination. ..
- Ruiz A, del Carmen Ruiz M, Sánchez Garrido M, Arino J, Ramos J. The Ppz protein phosphatases regulate Trk-independent potassium influx in yeast. FEBS Lett. 2004;578:58-62 pubmed
- Vavouri T, Semple J, Garcia Verdugo R, Lehner B. Intrinsic protein disorder and interaction promiscuity are widely associated with dosage sensitivity. Cell. 2009;138:198-208 pubmed publisher..Mass-action-driven interaction promiscuity is a single theoretical framework that can be used to understand, predict, and possibly treat the effects of increased gene expression in evolution and disease. ..
- Tio L, Pagani M, Atrian S. Drosophila proteins interacting with metallothioneins: a metal-dependent recognition. Proteomics. 2009;9:2568-77 pubmed publisher..Furthermore, new perspectives to investigate the often hypothesized contribution of MTs to the redox physiological networks are open. ..
- Ruiz A, González A, García Salcedo R, Ramos J, Arino J. Role of protein phosphatases 2C on tolerance to lithium toxicity in the yeast Saccharomyces cerevisiae. Mol Microbiol. 2006;62:263-77 pubmed..These results indicate that Ptc1p modulates the function of Ena1p by regulating the Hal3/Ppz1,2 pathway. In conclusion, overexpression of PTC3 and lack of PTC1 affect lithium tolerance in yeast, although through different mechanisms. ..
- Panicot M, Minguet E, Ferrando A, Alcázar R, Blázquez M, Carbonell J, et al. A polyamine metabolon involving aminopropyl transferase complexes in Arabidopsis. Plant Cell. 2002;14:2539-51 pubmed..Our data demonstrate the existence of a metabolon involving at least the last two steps of polyamine biosynthesis in Arabidopsis. ..
- Crosas E, Porté S, Moeini A, Farrés J, Biosca J, Parés X, et al. Novel alkenal/one reductase activity of yeast NADPH:quinone reductase Zta1p. Prospect of the functional role for the ?-crystallin family. Chem Biol Interact. 2011;191:32-7 pubmed publisher..The role of Zta1p in the yeast adaptation to some stress types and the general functional significance of ?-crystallins are discussed. ..
- Simon E, Clotet J, Calero F, Ramos J, Arino J. A screening for high copy suppressors of the sit4 hal3 synthetically lethal phenotype reveals a role for the yeast Nha1 antiporter in cell cycle regulation. J Biol Chem. 2001;276:29740-7 pubmed
- Clotet J, Posas F, de Nadal E, Arino J. The NH2-terminal extension of protein phosphatase PPZ1 has an essential functional role. J Biol Chem. 1996;271:26349-55 pubmed..These results indicates that the NH2-terminal half of the protein contains structural determinants that are specific for certain functions and that the phosphatase activity is required but not sufficient for full PPZ1 function. ..
- Marsellach F, Huertas D, Azorín F. The multi-KH domain protein of Saccharomyces cerevisiae Scp160p contributes to the regulation of telomeric silencing. J Biol Chem. 2006;281:18227-35 pubmed..These results are discussed in the context of a model in which Scp160p contributes to silencing by helping telomere clustering. ..
- Querol J, Rodríguez Concepción M, Boronat A, Imperial S. Essential role of residue H49 for activity of Escherichia coli 1-deoxy-D-xylulose 5-phosphate synthase, the enzyme catalyzing the first step of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid Synthesis. Biochem Biophys Res Commun. 2001;289:155-60 pubmed..These findings are consistent with those obtained for yeast TK and demonstrate a key role of H49 for DXS activity. ..
- Kizis D, Pages M. Maize DRE-binding proteins DBF1 and DBF2 are involved in rab17 regulation through the drought-responsive element in an ABA-dependent pathway. Plant J. 2002;30:679-89 pubmed..These results show that ABA plays a role in the regulation of DBF activity, and suggests the existence of an ABA-dependent pathway for the regulation of genes through the C-repeat/DRE element. ..
- Trigueros S, Roca J. Failure to relax negative supercoiling of DNA is a primary cause of mitotic hyper-recombination in topoisomerase-deficient yeast cells. J Biol Chem. 2002;277:37207-11 pubmed
- Clotet J, Gari E, Aldea M, Arino J. The yeast ser/thr phosphatases sit4 and ppz1 play opposite roles in regulation of the cell cycle. Mol Cell Biol. 1999;19:2408-15 pubmed
- Arino J. Integrative responses to high pH stress in S. cerevisiae. OMICS. 2010;14:517-23 pubmed publisher..In this review we will examine these responses, their possible interactions, and the role that they play in tolerance to high pH stress. ..
- Curwin A, von Blume J, Malhotra V. Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast. Mol Biol Cell. 2012;23:2327-38 pubmed publisher..These findings highlight the conserved role of cofilin and SPCA1/Pmr1 in sorting of the soluble secretory proteins at the TGN/late Golgi membranes in eukaryotes. ..
- Casado C, Yenush L, Melero C, Ruiz M, Serrano R, Perez Valle J, et al. Regulation of Trk-dependent potassium transport by the calcineurin pathway involves the Hal5 kinase. FEBS Lett. 2010;584:2415-20 pubmed publisher..This suggests that the control exerted by calcineurin on Hal5 expression may be biologically relevant for Trk1 regulation. ..
- Navarro Sastre A, Tort F, Stehling O, Uzarska M, Arranz J, Del Toro M, et al. A fatal mitochondrial disease is associated with defective NFU1 function in the maturation of a subset of mitochondrial Fe-S proteins. Am J Hum Genet. 2011;89:656-67 pubmed publisher..Importantly, yeast Nfu1 protein carrying the individuals' missense mutation was functionally impaired. We conclude that NFU1 functions as a late-acting maturation factor for a subset of mitochondrial Fe-S proteins. ..
- Erb I, van Nimwegen E. Transcription factor binding site positioning in yeast: proximal promoter motifs characterize TATA-less promoters. PLoS ONE. 2011;6:e24279 pubmed publisher..This close agreement between transcription factor binding site density and nucleosome depletion suggests a direct and general competition between transcription factors and nucleosomes for binding to promoters. ..
- Grötsch H, Giblin J, Idrissi F, Fernández Golbano I, Collette J, Newpher T, et al. Calmodulin dissociation regulates Myo5 recruitment and function at endocytic sites. EMBO J. 2010;29:2899-914 pubmed publisher..Concomitantly, calmodulin dissociation triggered Myo5 binding to Vrp1, extended the myosin-I lifespan at endocytic sites and activated Myo5-induced actin polymerization. ..
- Marfil V, Moya M, Pierreux C, Castell J, Lemaigre F, Real F, et al. Interaction between Hhex and SOX13 modulates Wnt/TCF activity. J Biol Chem. 2010;285:5726-37 pubmed publisher..We conclude that the interaction between Hhex and SOX13 may contribute to control Wnt/TCF signaling in the early embryo. ..
- Simon E, Barceló A, Arino J. Mutagenesis analysis of the yeast Nha1 Na+/H+ antiporter carboxy-terminal tail reveals residues required for function in cell cycle. FEBS Lett. 2003;545:239-45 pubmed..The later is rather conserved in other related antiporters, while the former is not. ..
- Alves Rodrigues I, Galao R, Meyerhans A, Diez J. Saccharomyces cerevisiae: a useful model host to study fundamental biology of viral replication. Virus Res. 2006;120:49-56 pubmed..Here, we review the different viruses able to direct replication and gene expression in yeast and discuss their main contributions in the understanding of virus biology. ..
- Gonzalez E, Fernández M, Marco D, Calam E, Sumoy L, Parés X, et al. Role of Saccharomyces cerevisiae oxidoreductases Bdh1p and Ara1p in the metabolism of acetoin and 2,3-butanediol. Appl Environ Microbiol. 2010;76:670-9 pubmed publisher..Furthermore, we have expressed Bdh2p with a histidine tag and have shown it to be inactive toward 2,3-butanediol. A whole-genome expression analysis with microarrays demonstrates that BDH1 and BDH2 are reciprocally regulated. ..
- de Nadal E, Posas F. Multilayered control of gene expression by stress-activated protein kinases. EMBO J. 2010;29:4-13 pubmed publisher..Thus, elucidating the control of gene expression by the Hog1 SAPK should help to understand how eukaryotic cells implement a massive and rapid change on their transcriptional capacity in response to adverse conditions. ..
- Vendrell A, Posas F. Sir2 plays a key role in cell fate determination upon SAPK activation. Aging (Albany NY). 2011;3:1163-8 pubmed..The conserved function of Sir2 in age-related diseases and the conserved role of SAPKs open the possibility of a novel role for sirtuins in cell fate determination in eukaryotic cells. ..
- Porté S, Crosas E, Yakovtseva E, Biosca J, Farrés J, Fernández M, et al. MDR quinone oxidoreductases: the human and yeast zeta-crystallins. Chem Biol Interact. 2009;178:288-94 pubmed publisher..In this context, the physiological role of zeta-crystallins is discussed. ..
- Bruns C, McCaffery J, Curwin A, Duran J, Malhotra V. Biogenesis of a novel compartment for autophagosome-mediated unconventional protein secretion. J Cell Biol. 2011;195:979-92 pubmed publisher..We describe the cellular proteins required for the biogenesis of CUPS, which we believe is the sorting station for Acb1's release from the cells...
- Neurohr G, Naegeli A, Titos I, Theler D, Greber B, Diez J, et al. A midzone-based ruler adjusts chromosome compaction to anaphase spindle length. Science. 2011;332:465-8 pubmed publisher..Thus, the anaphase spindle may function as a ruler to adapt the condensation of chromatids, promoting their segregation regardless of chromosome or spindle length. ..
- Rodriguez Hernandez C, Guinovart J, Murguia J. Anti-diabetic and anti-obesity agent sodium tungstate enhances GCN pathway activation through Glc7p inhibition. FEBS Lett. 2012;586:270-6 pubmed publisher..Therefore tungstate alleviates nutrient stress in yeast by in vivo inhibition of Glc7p. These data uncover a potential role for tungstate in the treatment of PP1 and GCN related diseases. ..
- Sabate R, Villar Piqué A, Espargaro A, Ventura S. Temperature dependence of the aggregation kinetics of Sup35 and Ure2p yeast prions. Biomacromolecules. 2012;13:474-83 pubmed publisher..Overall, we show here that the amyloidogenic pathways of Sup35 and Ure2p prions diverge significantly. ..
- Larroy C, Parés X, Biosca J. Characterization of a Saccharomyces cerevisiae NADP(H)-dependent alcohol dehydrogenase (ADHVII), a member of the cinnamyl alcohol dehydrogenase family. Eur J Biochem. 2002;269:5738-45 pubmed..Simultaneous deletion of ADH6 and ADH7 was not lethal for the yeast. Both enzymes could participate in the synthesis of fusel alcohols, ligninolysis and NADP(H) homeostasis. ..
- Morillas M, Clotet J, Rubi B, Serra D, Arino J, Hegardt F, et al. Inhibition by etomoxir of rat liver carnitine octanoyltransferase is produced through the co-ordinate interaction with two histidine residues. Biochem J. 2000;351 Pt 2:495-502 pubmed..Our data demonstrate that both etomoxir and malonyl-CoA inhibit COT by interacting with the same sites. ..
- Meyer M, Vilardell J. The quest for a message: budding yeast, a model organism to study the control of pre-mRNA splicing. Brief Funct Genomic Proteomic. 2009;8:60-7 pubmed publisher..This should provide a valuable system to approach the complex problem of the role of regulated splicing in genomic expression. ..
- Munoz I, Ruiz A, Marquina M, Barceló A, Albert A, Arino J. Functional characterization of the yeast Ppz1 phosphatase inhibitory subunit Hal3: a mutagenesis study. J Biol Chem. 2004;279:42619-27 pubmed..In contrast, the mutation of His378 did not. This finding suggests that Hal3 may have both Ppz1-dependent and independent functions involving different structural elements. ..
- Macias S, Bragulat M, Tardiff D, Vilardell J. L30 binds the nascent RPL30 transcript to repress U2 snRNP recruitment. Mol Cell. 2008;30:732-42 pubmed publisher..Collectively, our data suggest that L30 represses a spliceosomal rearrangement required for U2 snRNP association with the transcript. ..
- Serrano R, Ruiz A, Bernal D, Chambers J, Arino J. The transcriptional response to alkaline pH in Saccharomyces cerevisiae: evidence for calcium-mediated signalling. Mol Microbiol. 2002;46:1319-33 pubmed..These results demonstrate that the transcriptional response to alkaline pH involves different signalling mechanisms, and that calcium signalling is a relevant component of this response. ..
- Ruiz A, Munoz I, Serrano R, González A, Simon E, Arino J. Functional characterization of the Saccharomyces cerevisiae VHS3 gene: a regulatory subunit of the Ppz1 protein phosphatase with novel, phosphatase-unrelated functions. J Biol Chem. 2004;279:34421-30 pubmed..These results indicate that, besides its role as Ppz1 inhibitory subunit, Vhs3 (and probably Hal3) might have important Ppz-independent functions. ..
- González A, Ruiz A, Casamayor A, Arino J. Normal function of the yeast TOR pathway requires the type 2C protein phosphatase Ptc1. Mol Cell Biol. 2009;29:2876-88 pubmed publisher..All of these results provide the first evidence showing that a type 2C protein phosphatase is required for the normal functioning of the TOR pathway. ..
- Marquina M, González A, Barreto L, Gelis S, Munoz I, Ruiz A, et al. Modulation of yeast alkaline cation tolerance by Ypi1 requires calcineurin. Genetics. 2012;190:1355-64 pubmed publisher..These results point to a complex role of Ypi1 on the regulation of cation homeostasis, largely mediated by the calcineurin phosphatase. ..
- Duran J, Anjard C, Stefan C, Loomis W, Malhotra V. Unconventional secretion of Acb1 is mediated by autophagosomes. J Cell Biol. 2010;188:527-36 pubmed publisher..We propose that these autophagosome intermediates fuse with recycling endosomes instead to form multivesicular body carriers that then fuse with the plasma membrane to release cargo. ..
- de Nadal E, Calero F, Ramos J, Arino J. Biochemical and genetic analyses of the role of yeast casein kinase 2 in salt tolerance. J Bacteriol. 1999;181:6456-62 pubmed..The possible causes for the salt sensitivity phenotype of casein kinase mutants are discussed in the light of these findings. ..
- Mas G, de Nadal E, Dechant R, Rodríguez de la Concepción M, Logie C, Jimeno González S, et al. Recruitment of a chromatin remodelling complex by the Hog1 MAP kinase to stress genes. EMBO J. 2009;28:326-36 pubmed publisher..Our results suggest that the selective targeting of the RSC complex by Hog1 provides the necessary mechanistic basis for this event. ..
- Joshi R, Pina B, Roca J. Topoisomerase II is required for the production of long Pol II gene transcripts in yeast. Nucleic Acids Res. 2012;40:7907-15 pubmed publisher..Apparently, only topo II relaxes efficiently the (+) DNA supercoils that stall the advancement of Pol II in long genes. A mechanistic model is proposed. ..
- Bonet R, Ramirez Espain X, Macias M. Solution structure of the yeast URN1 splicing factor FF domain: comparative analysis of charge distributions in FF domain structures-FFs and SURPs, two domains with a similar fold. Proteins. 2008;73:1001-9 pubmed publisher..This cavity is also present in at least CA150FF1 and FF2 structures. Thus, as with WW domains, the FF fold seems to have developed binding-site variations to accommodate an abundant and variable set of ligands. ..
- Salceda J, Fernandez X, Roca J. Topoisomerase II, not topoisomerase I, is the proficient relaxase of nucleosomal DNA. EMBO J. 2006;25:2575-83 pubmed..We conclude that topoisomerase II is the main modulator of DNA topology in chromatin fibers. The nonessential topoisomerase I then assists DNA relaxation where chromatin structure impairs DNA juxtaposition but allows twist diffusion. ..
- de Nadal E, Clotet J, Posas F, Serrano R, Gomez N, Arino J. The yeast halotolerance determinant Hal3p is an inhibitory subunit of the Ppz1p Ser/Thr protein phosphatase. Proc Natl Acad Sci U S A. 1998;95:7357-62 pubmed..Therefore, Hal3p might modulate diverse physiological functions of the Ppz1 phosphatase, such as salt stress tolerance and cell cycle progression, by acting as a inhibitory subunit. ..
- Ruiz Roig C, Viéitez C, Posas F, de Nadal E. The Rpd3L HDAC complex is essential for the heat stress response in yeast. Mol Microbiol. 2010;76:1049-62 pubmed publisher..Finally, we found that the large, but not the small Rpd3 complex regulated cell adaptation in response to heat stress. ..
- Menoyo S, Ricco N, Bru S, Hernandez Ortega S, Escote X, Aldea M, et al. Phosphate-activated cyclin-dependent kinase stabilizes G1 cyclin to trigger cell cycle entry. Mol Cell Biol. 2013;33:1273-84 pubmed publisher..Taken together, our data indicate that Cln3 is a molecular target of the Pho85 kinase that is required to modulate cell cycle entry in response to environmental changes in nutrient availability. ..
- Nadal Ribelles M, Sol C, Xu Z, Steinmetz L, de Nadal E, Posas F. Control of Cdc28 CDK1 by a stress-induced lncRNA. Mol Cell. 2014;53:549-61 pubmed publisher..The increase in the levels of Cdc28 results in cells able to reenter the cell cycle more efficiently after stress. This may represent a general mechanism to prime expression of genes needed after stresses are alleviated...
- Thomson T, Khalid H, Lozano J, Sancho E, Arino J. Role of UEV-1A, a homologue of the tumor suppressor protein TSG101, in protection from DNA damage. FEBS Lett. 1998;423:49-52 pubmed..We also show that the mutant phenotype is fully complemented by expression of the human UEV-1A cDNA and we propose that UEV-1 proteins could also have a role in protecting higher eukaryotic cells from DNA damaging agents. ..
- Hernández Ortega S, Bru S, Ricco N, Ramirez S, Casals N, Jimenez J, et al. Defective in mitotic arrest 1 (Dma1) ubiquitin ligase controls G1 cyclin degradation. J Biol Chem. 2013;288:4704-14 pubmed publisher..This new previously unknown mechanism for G(1) cyclin degradation that we report here could help elucidate the specific roles of the redundant CDK-cyclin complexes in G(1). ..
- Calam E, GonzÃ¡lez Roca E, FernÃ¡ndez M, Dequin S, ParÃ©s X, Virgili A, et al. Enantioselective Synthesis of Vicinal (R,R)-Diols by Saccharomyces cerevisiae Butanediol Dehydrogenase. Appl Environ Microbiol. 2016;82:1706-1721 pubmed publisher..In conclusion, Bdh1p is an enzyme with biotechnological interest that can be used to synthesize chiral building blocks. A scheme of the favored pathway with the corresponding intermediates is proposed for the Bdh1p reaction. ..