Experts and Doctors on pseudomonas putida in Granada, Andalusia, Spain


Locale: Granada, Andalusia, Spain
Topic: pseudomonas putida

Top Publications

  1. Castillo J, Romero E, Nogales R. Dynamics of microbial communities related to biochemical parameters during vermicomposting and maturation of agroindustrial lignocellulose wastes. Bioresour Technol. 2013;146:345-354 pubmed publisher
    ..Alphaproteobacteria increased only in O (26%). Despite the different initial lignocellulose wastes, the mature vermicomposts were similar in microbial and biochemical properties. ..
  2. van Dillewijn P, Wittich R, Caballero A, Ramos J. Type II hydride transferases from different microorganisms yield nitrite and diarylamines from polynitroaromatic compounds. Appl Environ Microbiol. 2008;74:6820-3 pubmed publisher
  3. Segura A, Hurtado A, Rivera B, Lazaroaie M. Isolation of new toluene-tolerant marine strains of bacteria and characterization of their solvent-tolerance properties. J Appl Microbiol. 2008;104:1408-16 pubmed
    ..This type of bioreactor will require strains able to grow in a salt-containing environment and able to tolerate a second phase of an organic solvent. ..
  4. Fernandez M, Niqui Arroyo J, Conde S, Ramos J, Duque E. Enhanced tolerance to naphthalene and enhanced rhizoremediation performance for Pseudomonas putida KT2440 via the NAH7 catabolic plasmid. Appl Environ Microbiol. 2012;78:5104-10 pubmed publisher
  5. Molina L, Duque E, Gómez M, Krell T, Lacal J, García Puente A, et al. The pGRT1 plasmid of Pseudomonas putida DOT-T1E encodes functions relevant for survival under harsh conditions in the environment. Environ Microbiol. 2011;13:2315-27 pubmed publisher
    ..Furthermore, this plasmid encodes functions that allow the cells to chemotactically respond to toluene and participate in iron scavenging. ..
  6. Ramos J, Marques S, Timmis K. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annu Rev Microbiol. 1997;51:341-73 pubmed
    ..The two sigma54-dependent promoters are additionally subject to global (catabolite repression) control. ..
  7. Daddaoua A, Krell T, Alfonso C, Morel B, Ramos J. Compartmentalized glucose metabolism in Pseudomonas putida is controlled by the PtxS repressor. J Bacteriol. 2010;192:4357-66 pubmed publisher
    ..Footprint analysis revealed that PtxS binds to an almost perfect palindrome that is present within the three promoters and whose consensus sequence is 5'-TGAAACCGGTTTCA-3'. This palindrome overlaps with the RNA polymerase binding site...
  8. Pini C, Bernal P, Godoy P, Ramos J, Segura A. Cyclopropane fatty acids are involved in organic solvent tolerance but not in acid stress resistance in Pseudomonas putida DOT-T1E. Microb Biotechnol. 2009;2:253-61 pubmed publisher
    ..putida cfaB mutant was as tolerant to acid shock as the wild-type strain. The cfaB mutant was also as tolerant as the parental strain to a number of drugs, antibiotics and other damaging agents...
  9. Fernandez M, Conde S, De La Torre J, Molina Santiago C, Ramos J, Duque E. Mechanisms of resistance to chloramphenicol in Pseudomonas putida KT2440. Antimicrob Agents Chemother. 2012;56:1001-9 pubmed publisher
    ..Transcriptional arrays revealed that AgmR controls the expression of the pqq genes and the operon encoding the ABC extrusion pump from the promoter upstream of open reading frame (ORF) PP2669...

More Information


  1. Fernández Piñar R, Ramos J, Rodriguez Herva J, Espinosa Urgel M. A two-component regulatory system integrates redox state and population density sensing in Pseudomonas putida. J Bacteriol. 2008;190:7666-74 pubmed publisher
    ..A putative RoxR recognition element containing a conserved hexamer (TGCCAG) has also been identified in promoters of genes regulated by this two-component system. ..
  2. Matilla M, Travieso M, Ramos J, Ramos Gonzalez M. Cyclic diguanylate turnover mediated by the sole GGDEF/EAL response regulator in Pseudomonas putida: its role in the rhizosphere and an analysis of its target processes. Environ Microbiol. 2011;13:1745-66 pubmed publisher
    ..Cellulose and alginate were discarded as the overproduced EPS, and lipopolysaccharide (LPS) core and O-antigen were found to be essential for the development of wrinkly colony morphology. ..
  3. Fillet S, Krell T, Morel B, Lu D, Zhang X, Ramos J. Intramolecular signal transmission in a tetrameric repressor of the IclR family. Proc Natl Acad Sci U S A. 2011;108:15372-7 pubmed publisher
  4. Herrera M, Krell T, Zhang X, Ramos J. PhhR binds to target sequences at different distances with respect to RNA polymerase in order to activate transcription. J Mol Biol. 2009;394:576-86 pubmed publisher
    ..When functioning as an enhancer protein, PhhR-mediated transcription is modulated by the integration host factor protein. PhhR represses transcription from its own promoter and the promoter of the paaY gene by steric hindrance. ..
  5. Krell T, Lacal J, Guazzaroni M, Busch A, Silva Jímenez H, Fillet S, et al. Responses of Pseudomonas putida to toxic aromatic carbon sources. J Biotechnol. 2012;160:25-32 pubmed publisher
    ..Data suggest that transcriptional activation of the TOD pathway occurs at very low toluene concentrations whereas TtgV mediated induction of pump expression sets in as the toluene concentration further increases. ..
  6. Udaondo Z, Duque E, Fernandez M, Molina L, De La Torre J, Bernal P, et al. Analysis of solvent tolerance in Pseudomonas putida DOT-T1E based on its genome sequence and a collection of mutants. FEBS Lett. 2012;586:2932-8 pubmed publisher
    ..Local and global transcriptional regulators participate in a complex network of metabolic functions, acting as the decision makers in the response to solvents...
  7. Martínez Gil M, Romero D, Kolter R, Espinosa Urgel M. Calcium causes multimerization of the large adhesin LapF and modulates biofilm formation by Pseudomonas putida. J Bacteriol. 2012;194:6782-9 pubmed publisher
    ..putida cells interact with each other via LapF in a calcium-dependent manner during the development of biofilms. ..
  8. Mart nez Gil M, Ramos Gonz lez M, Espinosa Urgel M. Roles of cyclic Di-GMP and the Gac system in transcriptional control of the genes coding for the Pseudomonas putida adhesins LapA and LapF. J Bacteriol. 2014;196:1484-95 pubmed publisher
    ..This work represents a further step in our understanding of the regulatory interactions controlling biofilm formation in P. putida...
  9. Rodr guez Herva J, Ramos J. Characterization of an OprL null mutant of Pseudomonas putida. J Bacteriol. 1996;178:5836-40 pubmed
    ..The oprL gene was conserved in a wide variety of the Pseudomonas strains belonging to rRNA group I, which suggests that this gene is important for the maintenance of the cell envelope and cell morphology in this group of microorganisms...
  10. Ronchel M, Molina L, Witte A, Lutbiz W, Molin S, Ramos J, et al. Characterization of cell lysis in Pseudomonas putida induced upon expression of heterologous killing genes. Appl Environ Microbiol. 1998;64:4904-11 pubmed
    ..In both cases, cell death occurred as a result of impaired respiration, altered membrane permeability, and the release of some cytoplasmic contents to the extracellular medium. ..
  11. V lchez S, Molina L, Ramos C, Ramos J. Proline catabolism by Pseudomonas putida: cloning, characterization, and expression of the put genes in the presence of root exudates. J Bacteriol. 2000;182:91-9 pubmed
    ..putida put genes, as determined by using fusions of the put promoters to 'lacZ. The induction ratio for the putA promoter (about 20-fold) was 6-fold higher than the induction ratio for the putP promoter...
  12. Ronchel M, Ramos J. Dual system to reinforce biological containment of recombinant bacteria designed for rhizoremediation. Appl Environ Microbiol. 2001;67:2649-56 pubmed
    ..However, it disappeared in less than 20 to 25 days in soils without the pollutant, whereas an asd(+), biologically contained counterpart such as P. putida CMC4 was still detectable in soils after 100 days. ..
  13. Lacal J, Muñoz Martínez F, Reyes Darias J, Duque E, Matilla M, Segura A, et al. Bacterial chemotaxis towards aromatic hydrocarbons in Pseudomonas. Environ Microbiol. 2011;13:1733-44 pubmed publisher
    ..In vitro experiments show that McpT is methylated by CheR and McpT net methylation was diminished in the presence of hydrocarbons, what influences chemotactic movement towards these chemicals. ..
  14. Ramos J, Duque E, Godoy P, Segura A. Efflux pumps involved in toluene tolerance in Pseudomonas putida DOT-T1E. J Bacteriol. 1998;180:3323-9 pubmed
    ..The mutation in P. putida DOT-T1E-18 was cloned, and the knockedout gene was sequenced and found to be homologous to the drug exclusion gene mexB, which belongs to the efflux pump family of the resistant nodulator division type...
  15. Quesada J, Soriano M, Espinosa Urgel M. Stability of a Pseudomonas putida KT2440 bacteriophage-carried genomic island and its impact on rhizosphere fitness. Appl Environ Microbiol. 2012;78:6963-74 pubmed publisher
  16. Duque E, De La Torre J, Bernal P, Molina Henares M, Alaminos M, Espinosa Urgel M, et al. Identification of reciprocal adhesion genes in pathogenic and non-pathogenic Pseudomonas. Environ Microbiol. 2013;15:36-48 pubmed publisher
    ..In silico analysis showed this set of genes to be well conserved in all sequenced P. putida strains, and that at least eight reciprocal genes involved in attachment are shared by P. putida and P. aeruginosa...
  17. Alaminos M, Ramos J. The methionine biosynthetic pathway from homoserine in Pseudomonas putida involves the metW, metX, metZ, metH and metE gene products. Arch Microbiol. 2001;176:151-4 pubmed
    ..The latter reaction can be mediated by either of the two methionine synthetases present in the cells...
  18. van Dillewijn P, Wittich R, Caballero A, Ramos J. Subfunctionality of hydride transferases of the old yellow enzyme family of flavoproteins of Pseudomonas putida. Appl Environ Microbiol. 2008;74:6703-8 pubmed publisher
  19. Revelles O, Espinosa Urgel M, Fuhrer T, Sauer U, Ramos J. Multiple and interconnected pathways for L-lysine catabolism in Pseudomonas putida KT2440. J Bacteriol. 2005;187:7500-10 pubmed
    ..This study establishes an unequivocal relationship between gene and pathway enzymes in the metabolism of L-lysine, which is of crucial importance for the successful colonization of the rhizosphere of plants by this microorganism...
  20. Krell T, Ter n W, Mayorga O, Rivas G, Jim nez M, Daniels C, et al. Optimization of the palindromic order of the TtgR operator enhances binding cooperativity. J Mol Biol. 2007;369:1188-99 pubmed publisher
    ..Based on the architecture of the operator, the thermodynamics of the process, and the TtgR-operator interactions we propose a model for the binding of TtgR to its target sequence...
  21. Mart nez Gil M, Yousef Coronado F, Espinosa Urgel M. LapF, the second largest Pseudomonas putida protein, contributes to plant root colonization and determines biofilm architecture. Mol Microbiol. 2010;77:549-61 pubmed publisher
    ..These data and the localization of the protein at discrete spots in areas of contact between cells in biofilms suggest that LapF determines the establishment of cell-cell interactions during sessile growth...
  22. Fernandez M, Duque E, Pizarro Tobias P, van Dillewijn P, Wittich R, Ramos J. Microbial responses to xenobiotic compounds. Identification of genes that allow Pseudomonas putida KT2440 to cope with 2,4,6-trinitrotoluene. Microb Biotechnol. 2009;2:287-94 pubmed publisher
    ..The ORF PP1232 whose transcriptional level did not change in response to TNT affected growth in the presence of nitroaromatic compounds and it was found in a screening of 4000 randomly generated mutants. ..
  23. Caballero A, Esteve N ez A, Zylstra G, Ramos J. Assimilation of nitrogen from nitrite and trinitrotoluene in Pseudomonas putida JLR11. J Bacteriol. 2005;187:396-9 pubmed publisher
    ..Three mutants that had insertions in ntrC, nasT, and cnmA, which encode regulatory proteins, failed to grow on nitrite but grew on TNT, although slower than the wild type...
  24. Teran W, Felipe A, Segura A, Rojas A, Ramos J, Gallegos M. Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux pump is mediated by the drug binding repressor TtgR. Antimicrob Agents Chemother. 2003;47:3067-72 pubmed
    ..This is the first case in which the expression of a drug transporter of the resistance-nodulation-division family has been shown to be regulated directly by antibiotics. ..
  25. Pini C, Godoy P, Bernal P, Ramos J, Segura A. Regulation of the cyclopropane synthase cfaB gene in Pseudomonas putida KT2440. FEMS Microbiol Lett. 2011;321:107-14 pubmed publisher
    ..In this regard, in a cti-deficient mutant background, the levels of CFAs were similar to those in the parental one under the same conditions. ..
  26. Segura A, Rojas A, Hurtado A, Huertas M, Ramos J. Comparative genomic analysis of solvent extrusion pumps in Pseudomonas strains exhibiting different degrees of solvent tolerance. Extremophiles. 2003;7:371-6 pubmed publisher
    ..1% (v/v) toluene shock. We also studied the number and type of efflux pumps in different strains in comparison with the P. putida DOT-T1E strain...
  27. Herrera M, Daddaoua A, Fernández Escamilla A, Ramos J. Involvement of the global Crp regulator in cyclic AMP-dependent utilization of aromatic amino acids by Pseudomonas putida. J Bacteriol. 2012;194:406-12 pubmed publisher
    ..This nucleoprotein complex promotes transcription from phhA only in response to L-phenylalanine. ..
  28. Rodríguez Herva J, Reniero D, Galli E, Ramos J. Cell envelope mutants of Pseudomonas putida: physiological characterization and analysis of their ability to survive in soil. Environ Microbiol. 1999;1:479-88 pubmed
    ..putida to colonize soil. In comparison with the wild-type strain, the ability of single mutants to colonize soil decreased; this characteristic was more evident for the double mutant, especially at high temperatures. ..
  29. Ramos J, Krell T, Daniels C, Segura A, Duque E. Responses of Pseudomonas to small toxic molecules by a mosaic of domains. Curr Opin Microbiol. 2009;12:215-20 pubmed publisher
    ..A mosaic of domains has been recruited to sense and metabolize these chemicals in order to obtain energy and carbon. ..
  30. Fillet S, Velez M, Lu D, Zhang X, Gallegos M, Ramos J. TtgV represses two different promoters by recognizing different sequences. J Bacteriol. 2009;191:1901-9 pubmed publisher
    ..TtgV residues R47, R52, L57, and T49 are critical for binding to both operators. Based on three-dimensional models, we propose that these residues contact nucleotides within the major groove of DNA. ..
  31. Lacal J, Guazzaroni M, Gutiérrez del Arroyo P, Busch A, Velez M, Krell T, et al. Two levels of cooperativeness in the binding of TodT to the tod operon promoter. J Mol Biol. 2008;384:1037-47 pubmed publisher
    ..This hairpin seems important for transcriptional activation, since C-TodT binding to PtodX does not stimulate transcription. ..
  32. Busch A, Lacal J, Silva Jímenez H, Krell T, Ramos J. Catabolite repression of the TodS/TodT two-component system and effector-dependent transphosphorylation of TodT as the basis for toluene dioxygenase catabolic pathway control. J Bacteriol. 2010;192:4246-50 pubmed publisher
  33. Lacal J, Alfonso C, Liu X, Parales R, Morel B, Conejero Lara F, et al. Identification of a chemoreceptor for tricarboxylic acid cycle intermediates: differential chemotactic response towards receptor ligands. J Biol Chem. 2010;285:23126-36 pubmed publisher
    ..In this respect clear parallels exist to the Tar receptor and other eukaryotic receptors, which are discussed. ..
  34. Rodr guez Herva J, Ramos Gonzalez M, Ramos J. The Pseudomonas putida peptidoglycan-associated outer membrane lipoprotein is involved in maintenance of the integrity of the cell cell envelope. J Bacteriol. 1996;178:1699-706 pubmed
    ..putida. The protein was identified in coupled transcription and translation assays in vitro...