Genomes and Genes
Experts and Doctors on amino acid sequence in Dundee, KwaZulu Natal, South Africa
Locale: Dundee, KwaZulu Natal, South Africa
Topic: amino acid sequence
- Bammler T, Smith C, Wolf C. Isolation and characterization of two mouse Pi-class glutathione S-transferase genes. Biochem J. 1994;298 ( Pt 2):385-90 pubmed..Both genes are transcribed at significantly higher levels in male mouse liver than in female, and Gst p-1 mRNA is more abundant in both sexes than Gst p-2. ..
- Boxer D, Zhang H, Gourley D, Hunter W, Kelly S, Price N. Sensing of remote oxyanion binding at the DNA binding domain of the molybdate-dependent transcriptional regulator, ModE. Org Biomol Chem. 2004;2:2829-37 pubmed
- Helps N, Cohen P. Drosophila melanogaster protein phosphatase inhibitor-2: identification of a site important for PP1 inhibition. FEBS Lett. 1999;463:72-6 pubmed..Mutation of Phe residues conserved in I-2 from lower and higher eukaryotes showed that Phe-33 is important for inhibition of PP1c. ..
- Witte C, Isidore E, Tiller S, Davies H, Taylor M. Functional characterisation of urease accessory protein G (ureG) from potato. Plant Mol Biol. 2001;45:169-79 pubmed..The plant gene complements the K. aerogenes ureG mutation, demonstrating that it encodes a urease accessory protein and indicating a structural conservation between the plant and the bacterial urease activation complexes...
- Tetaud E, Giroud C, Prescott A, Parkin D, Baltz D, Biteau N, et al. Molecular characterisation of mitochondrial and cytosolic trypanothione-dependent tryparedoxin peroxidases in Trypanosoma brucei. Mol Biochem Parasitol. 2001;116:171-83 pubmed..In-situ immunofluorescence and immunoelectron microscopy revealed a colocalisation of TRYX and TRYP1 in the cytosol, whereas TRYP2 was principally localised in the mitochondrion...
- Peggie M, MacKelvie S, Bloecher A, Knatko E, Tatchell K, Stark M. Essential functions of Sds22p in chromosome stability and nuclear localization of PP1. J Cell Sci. 2002;115:195-206 pubmed..with Glc7p to promote dephosphorylation of nuclear substrates required for faithful transmission of chromosomes during mitosis, and this role is at least partly mediated by effects of Sds22p on the nuclear distribution of Glc7p ..
- Hensmann M, Li C, Moss C, Lindo V, Greer F, Watts C, et al. Disulfide bonds in merozoite surface protein 1 of the malaria parasite impede efficient antigen processing and affect the in vivo antibody response. Eur J Immunol. 2004;34:639-648 pubmed publisher..Although the reduced form of MSP1(19) does not induce protective antibodies, our data suggest that inclusion of unfolded protein may improve the efficacy of MSP1(19) as a vaccine...
- Turnbull Ross A, Reavy B, Mayo M, Murant A. The nucleotide sequence of parsnip yellow fleck virus: a plant picorna-like virus. J Gen Virol. 1992;73 ( Pt 12):3203-11 pubmed..The 3' untranslated region of PYFV RNA is 509 nts long and does not have a poly(A) tail. The 3'-terminal 121 nts may form a stem-loop structure which resembles that formed in the genomic RNA of mosquito-borne flaviviruses. ..
- Crouch D, Fisher F, La Rocca S, Goding C, Gillespie D. Viral mutations enhance the Max binding properties of the vMyc b-HLH-LZ domain. Nucleic Acids Res. 2005;33:5235-42 pubmed..We therefore propose that this virally derived mutation is functional by significantly contributing to establishing a more hydrophobic interface between the LZs of Myc and Max. ..
- Tiecke E, Bangs F, Blaschke R, Farrell E, Rappold G, Tickle C. Expression of the short stature homeobox gene Shox is restricted by proximal and distal signals in chick limb buds and affects the length of skeletal elements. Dev Biol. 2006;298:585-96 pubmed..These results fit well with the proposed role of Shox in cartilage and bone differentiation and suggest chick embryos as a useful model to study further the role of Shox in limb development. ..
- Scott J, Ross F, Liu J, Hardie D. Regulation of AMP-activated protein kinase by a pseudosubstrate sequence on the gamma subunit. EMBO J. 2007;26:806-15 pubmed..Binding of AMP causes a conformational change that prevents this interaction and relieves the inhibition. We present several lines of evidence supporting this hypothesis. ..
- Dawson A, Fyfe P, Hunter W. Specificity and reactivity in menaquinone biosynthesis: the structure of Escherichia coli MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate synthase). J Mol Biol. 2008;384:1353-68 pubmed publisher..This, in addition to structural and sequence comparisons with putative MenD orthologues, provides insight into the specificity and reactivity of MenD and allows a two-stage reaction mechanism to be proposed. ..
- Meunier I, Lenaers G, Bocquet B, Baudoin C, Piro Megy C, Cubizolle A, et al. A dominant mutation in MAPKAPK3, an actor of p38 signaling pathway, causes a new retinal dystrophy involving Bruch's membrane and retinal pigment epithelium. Hum Mol Genet. 2016;25:916-26 pubmed publisher..These findings shed new lights on Bruch's membrane/RPE pathophysiology and will open studies of this signaling pathway in diseases with RPE and Bruch's membrane alterations, such as age-related macular degeneration. ..
- Ziegler A, Natsuaki T, Mayo M, Jolly C, Murant A. The nucleotide sequence of RNA-1 of raspberry bushy dwarf virus. J Gen Virol. 1992;73 ( Pt 12):3213-8 pubmed..These findings support the idea that RBDV should be placed in a distinct virus genus for which the name idaeovirus has been proposed. ..
- Hong Y, Harrison B. Nucleotide sequences from tomato leaf curl viruses from different countries: evidence for three geographically separate branches in evolution of the coat protein of whitefly-transmitted geminiviruses. J Gen Virol. 1995;76 ( Pt 8):2043-9 pubmed..Indeed, the virus isolates causing similar diseases of tomato plants in the different continents are, with few exceptions, not closely related and warrant recognition as separate species. ..
- Turnbull Ross A, Mayo M, Reavy B, Murant A. Sequence analysis of the parsnip yellow fleck virus polyprotein: evidence of affinities with picornaviruses. J Gen Virol. 1993;74 ( Pt 4):555-61 pubmed..This justifies placing it in a separate plant virus genus for which the name 'sequivirus' has been proposed. ..
- Mann D, Dombradi V, Cohen P. Drosophila protein phosphatase V functionally complements a SIT4 mutant in Saccharomyces cerevisiae and its amino-terminal region can confer this complementation to a heterologous phosphatase catalytic domain. EMBO J. 1993;12:4833-42 pubmed..This increase in PPV correlates with introduction of the G2 phase of the cell cycle, elevated zygotic transcription and cellularization, indicating that PPV may play a role in one or more of these processes. ..
- Taylor M, George L, Ross H, Davies H. cDNA cloning and characterisation of an alpha-glucosidase gene from potato (Solanum tuberosum L.). Plant J. 1998;13:419-25 pubmed..A range of maltodextrins were substrates for this activity. The steady-state expression level of the potato alpha-glucosidase gene was low in most tissues examined, the highest levels occurring in sprouting tubers and source leaves. ..
- Jupe F, Pritchard L, Etherington G, Mackenzie K, Cock P, Wright F, et al. Identification and localisation of the NB-LRR gene family within the potato genome. BMC Genomics. 2012;13:75 pubmed publisher..Furthermore, the data provide a blueprint for future efforts to identify and more rapidly clone functional NB-LRR genes from Solanum species. ..
- Shehata S, Hunter R, Ohta E, Peggie M, Lou H, Sicheri F, et al. Analysis of substrate specificity and cyclin Y binding of PCTAIRE-1 kinase. Cell Signal. 2012;24:2085-94 pubmed publisher..Our understanding of the molecular interaction between PCTAIRE-1 and cyclin Y further facilitates future investigation of the functions of PCTAIRE-1 kinase. ..
- Mayo M, Robinson D, Jolly C, Hyman L. Nucleotide sequence of potato leafroll luteovirus RNA. J Gen Virol. 1989;70 ( Pt 5):1037-51 pubmed publisher..The nucleotide sequences reported will appear in the EMBL, GenBank and DDBJ databases under the accession number X14600...
- Hamilton G, Colbert J, Schuettelkopf A, Watts C. Cystatin F is a cathepsin C-directed protease inhibitor regulated by proteolysis. EMBO J. 2008;27:499-508 pubmed publisher..Thus, cystatin F is a latent protease inhibitor itself regulated by proteolysis in the endocytic pathway. By targeting cathepsin C, it may regulate diverse immune cell effector functions. ..
- Stevenson L, Sparks A, Allende Vega N, Xirodimas D, Lane D, Saville M. The deubiquitinating enzyme USP2a regulates the p53 pathway by targeting Mdm2. EMBO J. 2007;26:976-86 pubmed..Our data identify the deubiquitinating enzyme USP2a as a novel regulator of the p53 pathway that acts through its ability to selectively target Mdm2. ..
- Simpson G, Clark G, Rothnie H, Boelens W, van Venrooij W, Brown J. Molecular characterization of the spliceosomal proteins U1A and U2B" from higher plants. EMBO J. 1995;14:4540-50 pubmed
- Moss C, Villadangos J, Watts C. Destructive potential of the aspartyl protease cathepsin D in MHC class II-restricted antigen processing. Eur J Immunol. 2005;35:3442-51 pubmed..Our results indicate redundancy among lysosomal aspartyl proteases and show that while processing activities can be productive for MHC class II T cell epitope generation at one level, they can become destructive above an optimal level. ..
- Henderson C, Bammler T, Wolf C. Deduced amino acid sequence of a murine cytochrome P-450 Cyp4a protein: developmental and hormonal regulation in liver and kidney. Biochim Biophys Acta. 1994;1200:182-90 pubmed
- Lizcano J, Göransson O, Toth R, Deak M, Morrice N, Boudeau J, et al. LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1. EMBO J. 2004;23:833-43 pubmed..Between them, these kinases may mediate the physiological effects of LKB1, including its tumour suppressor function. ..
- Barker H, Brewis N, Street A, Spurr N, Cohen P. Three genes for protein phosphatase 1 map to different human chromosomes: sequence, expression and gene localisation of protein serine/threonine phosphatase 1 beta (PPP1CB). Biochim Biophys Acta. 1994;1220:212-8 pubmed..These data show that the genes for three protein phosphatase catalytic subunits PP1 alpha, PP1 beta, PP1 gamma are all located on different chromosomes. ..
- West M, Antoniou A, Prescott A, Azuma T, Kwiatkowski D, Watts C. Membrane ruffling, macropinocytosis and antigen presentation in the absence of gelsolin in murine dendritic cells. Eur J Immunol. 1999;29:3450-5 pubmed..Thus the major rearrangements of the actin cytoskeleton needed for DC antigen uptake and presentation can proceed in the absence of a major actin filament regulatory protein. ..
- Miller J, Wesley S, Naidu R, Reddy D, Mayo M. The nucleotide sequence of RNA-1 of Indian peanut clump furovius. Arch Virol. 1996;141:2301-12 pubmed..Oligonucleotide primers, designed on the basis of the sequences of RNA-1 of IPCV and PCV, were effective in reverse transcription-PCR amplification of these RNAs and that of IPCV isolates of the serologically distinct L and T serotypes. ..
- Groom L, Sneddon A, Alessi D, Dowd S, Keyse S. Differential regulation of the MAP, SAP and RK/p38 kinases by Pyst1, a novel cytosolic dual-specificity phosphatase. EMBO J. 1996;15:3621-32 pubmed
- Rugg E, Baty D, Shemanko C, Magee G, Polak S, Bergman R, et al. DNA based prenatal testing for the skin blistering disorder epidermolysis bullosa simplex. Prenat Diagn. 2000;20:371-7 pubmed..These results demonstrate the feasibility of DNA-based prenatal testing for EBS in families where causative mutations can be found. ..
- Hardie D, Carling D. The AMP-activated protein kinase--fuel gauge of the mammalian cell?. Eur J Biochem. 1997;246:259-73 pubmed..AMPK/SNF1 homologues are found in higher plants, and this protein-kinase cascade appears to be an ancient system which evolved to protect cells against the effects of nutritional or environmental stress. ..
- Taliansky M, Robinson D, Murant A. Complete nucleotide sequence and organization of the RNA genome of groundnut rosette umbravirus. J Gen Virol. 1996;77 ( Pt 9):2335-45 pubmed publisher..In genome organization and in the amino acid sequences of its potential products, the RNA of GRV is similar to that of carrot mottle mimic umbravirus, and to the umbravirus-like RNA-2 of pea enation mosaic virus...
- Goudie D, D Alessandro M, Merriman B, Lee H, Szever nyi I, Avery S, et al. Multiple self-healing squamous epithelioma is caused by a disease-specific spectrum of mutations in TGFBR1. Nat Genet. 2011;43:365-9 pubmed publisher..This distinguishes MSSE from the Marfan syndrome-related disorders in which missense mutations in TGFBR1 lead to developmental defects with vascular involvement but no reported predisposition to cancer...
- Guymer D, Maillard J, Agacan M, Brearley C, Sargent F. Intrinsic GTPase activity of a bacterial twin-arginine translocation proofreading chaperone induced by domain swapping. FEBS J. 2010;277:511-25 pubmed publisher..A D68W variant TorD protein was found to exhibit an ATPase activity not observed for native TorD, and an in vivo assay established that this variant was defective in the Tat proofreading process. ..
- Sneddon A, Cohen P, Stark M. Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genes. EMBO J. 1990;9:4339-46 pubmed..Measurement of type 2A protein phosphatase activity in yeast strains lacking one or other of the genes indicates that they account for most, if not all, protein phosphatase 2A activity in the cell. ..
- Walker K, Watt P, Cohen P. Phosphorylation of the skeletal muscle glycogen-targetting subunit of protein phosphatase 1 in response to adrenaline in vivo. FEBS Lett. 2000;466:121-4 pubmed..Thus the phosphorylation of G(M) at Ser48 by MAPKAP-K1 or other insulin-stimulated protein kinases is not involved in the activation of glycogen synthase by insulin. ..
- Belelli D, Balcarek J, Hope A, Peters J, Lambert J, Blackburn T. Cloning and functional expression of a human 5-hydroxytryptamine type 3AS receptor subunit. Mol Pharmacol. 1995;48:1054-62 pubmed
- Hope A, Downie D, Sutherland L, Lambert J, Peters J, Burchell B. Cloning and functional expression of an apparent splice variant of the murine 5-HT3 receptor A subunit. Eur J Pharmacol. 1993;245:187-92 pubmed..Upon expression in Xenopus oocytes, the homo-oligomeric receptor displayed pharmacological properties which define it as a functional 5-HT3 receptor. ..
- Chen M, McPartlin A, Brown L, Chen Y, Barker H, Cohen P. A novel human protein serine/threonine phosphatase, which possesses four tetratricopeptide repeat motifs and localizes to the nucleus. EMBO J. 1994;13:4278-90 pubmed..This suggests that, like other nuclear TPR-containing proteins, it may play a role in the regulation of RNA biogenesis and/or mitosis. ..
- Ariza A, Richard D, White M, Bond C. Conformational flexibility revealed by the crystal structure of a crenarchaeal RadA. Nucleic Acids Res. 2005;33:1465-73 pubmed..Additional analysis reveals that the interaction specificity of the five human Rad51 paralogues can be predicted using a simple model based on the RadA structure. ..
- Machray G, Burch L, Hedley P, Davies H, Waugh R. Characterisation of a complementary DNA encoding a novel plant enzyme with sucrolytic activity. FEBS Lett. 1994;354:123-7 pubmed..Expression of the cDNA in E. coli yields a fusion protein with sucrolytic activity. ..
- Pasare S, Wright K, Campbell R, Morris W, Ducreux L, Chapman S, et al. The sub-cellular localisation of the potato (Solanum tuberosum L.) carotenoid biosynthetic enzymes, CrtRb2 and PSY2. Protoplasma. 2013;250:1381-92 pubmed publisher..We conclude that it is important to consider the location of biosynthetic enzymes when engineering the carotenoid metabolic pathway in storage organs such as tubers. ..
- Terron Kwiatkowski A, Paller A, Compton J, Atherton D, McLean W, Irvine A. Two cases of primarily palmoplantar keratoderma associated with novel mutations in keratin 1. J Invest Dermatol. 2002;119:966-71 pubmed..These mutations appear to have a less damaging effect than previously reported mis-sense mutations sited in the helix boundary motifs. This report extends the range of phenotypes associated with mutations in KRT1. ..
- Mehellou Y, Alessi D, MacArtney T, Szklarz M, Knapp S, Elkins J. Structural insights into the activation of MST3 by MO25. Biochem Biophys Res Commun. 2013;431:604-9 pubmed publisher..These data provide molecular understanding of the mechanism by which MO25 isoforms regulates the activity of STE20 family protein kinases. ..
- Liu W, Midgley C, Stephen C, Saville M, Lane D. Biological significance of a small highly conserved region in the N terminus of the p53 tumour suppressor protein. J Mol Biol. 2001;313:711-31 pubmed..In support of this, the deletion was found to induce second-site reversion of the Val135 temperature-sensitive mutant of murine p53. ..
- English G, Trunk K, Rao V, Srikannathasan V, Hunter W, Coulthurst S. New secreted toxins and immunity proteins encoded within the Type VI secretion system gene cluster of Serratia marcescens. Mol Microbiol. 2012;86:921-36 pubmed publisher..marcescens T6SS, before horizontal acquisition of other T6SS-secreted toxins. Molecular insight has been provided into how pathogens utilize antibacterial T6SSs to overcome competitors and succeed in polymicrobial niches...
- Mann D, Campbell D, McGowan C, Cohen P. Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and comparative analysis of amino acid sequences. Biochim Biophys Acta. 1992;1130:100-4 pubmed
- Thorpe P, Cock P, Bos J. Comparative transcriptomics and proteomics of three different aphid species identifies core and diverse effector sets. BMC Genomics. 2016;17:172 pubmed publisher..Insights into the overlap and differences in aphid effector repertoires are important in understanding how different species successfully infest different ranges of plant species. ..
- Park J, Al Ramahi I, Tan Q, Mollema N, Diaz Garcia J, Gallego Flores T, et al. RAS-MAPK-MSK1 pathway modulates ataxin 1 protein levels and toxicity in SCA1. Nature. 2013;498:325-331 pubmed publisher..Collectively, these data reveal new therapeutic entry points for SCA1 and provide a proof-of-principle for tackling other classes of intractable neurodegenerative diseases. ..
- Ryan D, Sundaramoorthy R, Martin D, Singh V, Owen Hughes T. The DNA-binding domain of the Chd1 chromatin-remodelling enzyme contains SANT and SLIDE domains. EMBO J. 2011;30:2596-609 pubmed publisher..Point mutations to conserved amino acids within the human CHD7 SLIDE domain have been identified in patients with CHARGE syndrome. ..
- Hardie D. Regulation of fatty acid and cholesterol metabolism by the AMP-activated protein kinase. Biochim Biophys Acta. 1992;1123:231-8 pubmed
- McPartlin A, Barker H, Cohen P. Identification of a third alternatively spliced cDNA encoding the catalytic subunit of protein phosphatase 2B beta. Biochim Biophys Acta. 1991;1088:308-10 pubmed..The deletion results in the loss of ten amino acids between the putative calmodulin site and a postulated autoinhibitory domain. An identical deletion occurs in one of the two alternatively spliced isoforms of PP2B alpha. ..
- Cartlidge R, Knebel A, Peggie M, Alexandrov A, Phizicky E, Cohen P. The tRNA methylase METTL1 is phosphorylated and inactivated by PKB and RSK in vitro and in cells. EMBO J. 2005;24:1696-705 pubmed..In contrast, expression of METTL1 or METTL1[S27A] partially rescued growth. These results demonstrate that METTL1 is inactivated by PKB and RSK in cells, and the potential implications of this finding are discussed. ..
- Fukuzawa M, Williams J. OSBPa, a predicted oxysterol binding protein of Dictyostelium, is required for regulated entry into culmination. FEBS Lett. 2002;527:37-42 pubmed..Thus a plasma membrane-enriched OSBP family member is essential for correct regulation of the slug-fruiting body switch. ..
- Yin Y, Stephen C, Luciani M, Fahraeus R. p53 Stability and activity is regulated by Mdm2-mediated induction of alternative p53 translation products. Nat Cell Biol. 2002;4:462-7 pubmed..Thus, Mdm2 controls the expression levels of p53 through a dual mechanism that involves induction of synthesis and targeting for degradation. ..
- Nicolas F, Moore W, Zhang C, Clarke P. XMog1, a nuclear ran-binding protein in Xenopus, is a functional homologue of Schizosaccharomyces pombe mog1p that co-operates with RanBP1 to control generation of Ran-GTP. J Cell Sci. 2001;114:3013-23 pubmed..We propose that Mog1-related proteins, together with RanBP1, facilitate the generation of Ran-GTP from Ran-GDP in the nucleus. ..
- Alessi D, Kozlowski M, Weng Q, Morrice N, Avruch J. 3-Phosphoinositide-dependent protein kinase 1 (PDK1) phosphorylates and activates the p70 S6 kinase in vivo and in vitro. Curr Biol. 1998;8:69-81 pubmed..PDK1 is one of the components of the signaling pathway recruited by Pl 3-kinase for the activation of p70 S6 kinase as well as of PKB, and serves as a multifunctional effector downstream of the Pl 3-kinase. ..
- Dumaz N, Milne D, Meek D. Protein kinase CK1 is a p53-threonine 18 kinase which requires prior phosphorylation of serine 15. FEBS Lett. 1999;463:312-6 pubmed