Experts and Doctors on two hybrid system techniques in Seoul, South Korea


Locale: Seoul, South Korea
Topic: two hybrid system techniques

Top Publications

  1. Kang K, Kim J, Yong T, Park S. Identification of end-binding 1 (EB1) interacting proteins in Giardia lamblia. Parasitol Res. 2010;106:723-8 pubmed publisher
    ..lamblia. These results suggest that EB1 protein functions via interaction with the cytoskeletal components of G. lamblia. ..
  2. Choi Y, Kim S, Park K, Choi K. Differential transmission of G1 cell cycle arrest and mating signals by Saccharomyces cerevisiae Ste5 mutants in the pheromone pathway. Biochem Cell Biol. 1999;77:459-68 pubmed
    ..cerevisiae. In addition, the roles of Asp-248 and Tyr-421, which are important for pheromone signal transduction were further characterized by site-directed mutagenesis studies. ..
  3. Kim W, Lee H, Park E, Park J, Park S. Gain- and loss-of-function of Rhp51, a Rad51 homolog in fission yeast, reveals dissimilarities in chromosome integrity. Nucleic Acids Res. 2001;29:1724-32 pubmed
    ..The latter also exhibited MMS and UV sensitivity, indicating that Rhp51 K155A has a dominant negative effect. These results suggest an association between DNA replication and Rad51 function. ..
  4. Cho Y, Kim E, Park U, Sin H, Um S. Additional sex comb-like 1 (ASXL1), in cooperation with SRC-1, acts as a ligand-dependent coactivator for retinoic acid receptor. J Biol Chem. 2006;281:17588-98 pubmed
    ..Overall, these results suggest that ASXL1 is a novel coactivator of RAR that cooperates with SRC-1 and implicates it as a potential antitumor target of RA in RA-resistant cancer cells. ..
  5. Shin J, Gu C, Park E, Park S. Identification of phosphotyrosine binding domain-containing proteins as novel downstream targets of the EphA8 signaling function. Mol Cell Biol. 2007;27:8113-26 pubmed
    ..Taken together, our findings support a possible function for Anks family proteins as scaffolding proteins of the EphA8 signaling pathway. ..
  6. Kim J, Song K. The study of Bfa1p(E438K) suggests that Bfa1 control the mitotic exit network in different mechanisms depending on different checkpoint-activating signals. Mol Cells. 2006;21:251-60 pubmed
    ..In addition, the viability of cdc5-2 delta bfa1 cells was not reduced by BFA1(E438K), suggesting that Cdc5p also regulates Bfa1p to activate mitotic exit by other mechanism(s), besides phosphorylation. ..
  7. Shin D, Chun Y, Lee K, Shin H, Park J. Arrest defective-1 controls tumor cell behavior by acetylating myosin light chain kinase. PLoS ONE. 2009;4:e7451 pubmed publisher
    ..Thus, hARD1 could be a therapeutic target in the context of preventing tumor invasion and metastasis. ..
  8. Moon H, Ahn M, Park J, Min K, Kwon Y, Kim K. Negative regulation of hypoxia inducible factor-1alpha by necdin. FEBS Lett. 2005;579:3797-801 pubmed
    ..Collectively, these results suggest that necdin can be a novel negative regulator of HIF-1alpha stability via the direct interaction. ..
  9. Song H, Park J, Seo S, Kim J, Paik S, Chung K. Down syndrome critical region 2 protein inhibits the transcriptional activity of peroxisome proliferator-activated receptor beta in HEK293 cells. Biochem Biophys Res Commun. 2008;376:478-82 pubmed publisher
    ..DSCR2 also inhibited the ligand-induced transcriptional activity of PPARbeta. Furthermore, PPARbeta also decreased the solubility of DSCR2, which increased levels of insoluble DSCR2. ..

More Information


  1. Choi S, Park H. The novel peptide F29 facilitates the DNA-binding ability of hypoxia-inducible factor-1alpha. BMB Rep. 2009;42:737-42 pubmed
    ..Our results suggest that F29 can be a lead compound that directly targets HIF-1alpha and increases its activity. ..
  2. Shim E, Moon C, Lee G, Ha Y, Chae S, Lee J. Association of the Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD (SLP-76) with the p85 subunit of phosphoinositide 3-kinase. FEBS Lett. 2004;575:35-40 pubmed
    ..Collectively, these data suggest that SLP-76 may play a role in PI3K signaling pathways. ..
  3. Kim E, Song J, Kim D, Chater K, Lee K. A possible extended family of regulators of sigma factor activity in Streptomyces coelicolor. J Bacteriol. 2008;190:7559-66 pubmed publisher
    ..It appears that streptomycetes may possess an extraordinary abundance of anti-sigma factors, some of which may influence diverse processes through interactions with multiple partners: a novel feature for such regulatory proteins...
  4. Kim M, Go Y, Lee S, Kim Y, Shin J, Min M, et al. Seed-expressed casein kinase I acts as a positive regulator of the SeFAD2 promoter via phosphorylation of the SebHLH transcription factor. Plant Mol Biol. 2010;73:425-37 pubmed publisher
    ..This co-expression revealed that SeCKI enhanced the SebHLH-mediated transactivation of the SeFAD2 gene promoter via phosphorylation of the SebHLH transcription factor...
  5. Choi Y, Son M, Park M, Shin J, Yun Y. SOCS-6 negatively regulates T cell activation through targeting p56lck to proteasomal degradation. J Biol Chem. 2010;285:7271-80 pubmed publisher
    ..Moreover, SOCS-6 overexpression led to repression of TCR-dependent interleukin-2 promoter activity. These results establish that SOCS-6 acts as a negative regulator of T cell activation by promoting ubiquitin-dependent proteolysis. ..
  6. Lee C, Jeong S, Kim B, Choi C, Kim J, Koo B, et al. Dictyostelium CBP3 associates with actin cytoskeleton and is related to slug migration. Biochim Biophys Acta. 2005;1743:281-90 pubmed
    ..Taken together, these results suggest that CBP3 interacts with actin cytoskeleton and has a role during cell aggregation and slug migration of Dictyostelium. ..
  7. Hahn J. Regulation of Nod1 by Hsp90 chaperone complex. FEBS Lett. 2005;579:4513-9 pubmed
    ..These findings suggest a common regulatory mechanism involving the Hsp90 chaperone complex in R-mediated disease resistance in plants and Nod1-mediated innate immune response in mammals. ..
  8. Lee K, Pisarska M, Ko J, Kang Y, Yoon S, Ryou S, et al. Transcriptional factor FOXL2 interacts with DP103 and induces apoptosis. Biochem Biophys Res Commun. 2005;336:876-81 pubmed
    ..Our results present previously undiscovered functions of these proteins, an apoptotic activity of FOXL2 in the ovary and a modulating activity of DP103 by interacting with FOXL2. ..
  9. Hong S, Choi G, Park S, Chung A, Hunter E, Rhee S. Type D retrovirus Gag polyprotein interacts with the cytosolic chaperonin TRiC. J Virol. 2001;75:2526-34 pubmed
    ..Consequently, properly folded Gag molecules carry out the intermolecular interactions involved in self-assembly of the immature capsid. ..
  10. Jang Y, Park H, Kim S, Lee J, Suh M, Chung Y, et al. Survey of rice proteins interacting with OsFCA and OsFY proteins which are homologous to the Arabidopsis flowering time proteins, FCA and FY. Plant Cell Physiol. 2009;50:1479-92 pubmed publisher
    ..Taken together, our data suggest that OsFCA could interact with several proteins other than OsFY through its WW domain and may play several roles in rice. ..
  11. Park U, Kim E, Um S. A novel cytoplasmic adaptor for retinoic acid receptor (RAR) and thyroid receptor functions as a Derepressor of RAR in the absence of retinoic acid. J Biol Chem. 2010;285:34269-78 pubmed publisher
    ..CART1 siRNA treatment in a mouse Sertoli cell line, TM4, allowed RAR to move to the nucleus and blocked the derepressing function of CART1, suggesting that CART1 might be a cytoplasmic, testis-specific derepressor of RAR. ..
  12. Kim K, Kim T, Kang K, Choi K. Amphiphysin IIb-1, a novel splicing variant of amphiphysin II, regulates p73beta function through protein-protein interactions. Oncogene. 2001;20:6689-99 pubmed
    ..These findings offer a new insight into the regulation mechanism of p73beta, and suggest that amphiphysin IIb-1 modulates p73beta function by direct binding. ..
  13. Kim H, Kim B, Soh J, Cho E, Liu J, Youn H. A novel function of Nur77: physical and functional association with protein kinase C. Biochem Biophys Res Commun. 2006;348:950-6 pubmed
    ..Therefore, these findings demonstrate a novel function of Nur77 as a PKC inhibitor and give insights into molecular mechanisms of various Nur77-mediated physiological phenomena. ..
  14. Park M, Park S, Cho S, Kim K. Nicotiana benthamiana protein, NbPCIP1, interacting with Potato virus X coat protein plays a role as susceptible factor for viral infection. Virology. 2009;386:257-69 pubmed publisher
    ..Transient over-expression and transgene silencing of NbPCIP1 assay analysis indicated that NbPCIP1 plays a critical role in viral replication during PVX infection in host plant. ..
  15. Kim H, Yang D, So I. PDZ domain-containing protein as a physiological modulator of TRPV6. Biochem Biophys Res Commun. 2007;361:433-8 pubmed
    ..Knocking down the endogenous PDZK2 of HEK293 cells by RNAi significantly decreased DVF current density. Taken together, we propose that PDZK2 is an essential TRPV6 interacting protein as a physiological modulator of TRPV6. ..
  16. Park D, Yun Y. Tyrosine phosphorylation-dependent yeast two-hybrid system for the identification of the SH2 domain-binding proteins. Mol Cells. 2001;12:244-9 pubmed
    ..The conclusion is that the established tyrosine phosphorylation-dependent yeast two-hybrid system provides a novel and efficient way to define the SH2 domain-binding molecules. ..
  17. Lee D, Kang J, Park H, Kim M, Yim T, Kim J, et al. FBI-1 enhances transcription of the nuclear factor-kappaB (NF-kappaB)-responsive E-selectin gene by nuclear localization of the p65 subunit of NF-kappaB. J Biol Chem. 2005;280:27783-91 pubmed
    ..FBI-1 also interacted with IkappaB alpha and IkappaB beta. ..
  18. Ryu H, Kim S, Park H, You J, Kim B, Lee J, et al. Modulations of AtGSTF10 expression induce stress tolerance and BAK1-mediated cell death. Biochem Biophys Res Commun. 2009;379:417-22 pubmed publisher
  19. Kim O, Jung J, Park C. An Arabidopsis F-box protein regulates tapetum degeneration and pollen maturation during anther development. Planta. 2010;232:353-66 pubmed publisher
    ..It physically interacts with the Arabidopsis-Skp1-like1 protein via the F-box motif. These observations indicate that the RMF gene encodes an F-box protein functioning in tapetum degeneration during anther development. ..
  20. Joe M, Sohn S, Choi Y, Park H, Kee C. Identification of flotillin-1 as a protein interacting with myocilin: implications for the pathogenesis of primary open-angle glaucoma. Biochem Biophys Res Commun. 2005;336:1201-6 pubmed
    ..Although the physiological significance of the interaction has yet to be elucidated, our results showed that the alteration of the interaction by mutations in MYOC might be a key factor of the pathogenesis of POAG. ..
  21. Jeon B, Choi W, Yu M, Yoon A, Kim M, Yun C, et al. ZBTB2, a novel master regulator of the p53 pathway. J Biol Chem. 2009;284:17935-46 pubmed publisher
    ..Overall, our data suggest that ZBTB2 is a potential proto-oncogenic master control gene of the p53 pathway and, in particular, is a potent transcription repressor of the cell cycle arrest gene p21 by inhibiting p53 and Sp1. ..
  22. Lee S, Choi D, Rhim H, Choo H, Ko Y, Kim C, et al. PHB2 interacts with RNF2 and represses CP2c-stimulated transcription. Mol Cell Biochem. 2008;319:69-77 pubmed publisher
    ..Our study suggests a novel mechanism by which RNF2 and PHB2 modulate the CP2-mediated transcriptional pathway. ..
  23. Lee S, Lee J, Maeng Y, Kim Y, Kwon Y. Langerhans cell protein 1 (LCP1) binds to PNUTS in the nucleus: implications for this complex in transcriptional regulation. Exp Mol Med. 2009;41:189-200 pubmed publisher
    ..These findings suggest that the coordinated spatial and temporal regulation of LCP1 and PNUTS may be a novel mechanism to control the expression of genes that are critical for certain physiological and pathological processes. ..
  24. Kim H, Jeong W, Ahn K, Ahn C, Kang S. Siah-1 interacts with the intracellular region of polycystin-1 and affects its stability via the ubiquitin-proteasome pathway. J Am Soc Nephrol. 2004;15:2042-9 pubmed
    ..These results suggest that polycystin-1 is regulated by Siah-1 through the ubiquitin-dependent proteasome pathway. ..
  25. Oh S, Park J, Yoon S, Kim Y, Park S, Ryu M, et al. The Arabidopsis calcium sensor calcineurin B-like 3 inhibits the 5'-methylthioadenosine nucleosidase in a calcium-dependent manner. Plant Physiol. 2008;148:1883-96 pubmed publisher
    ..We also discuss a possible mechanism by which the CBL3-mediated calcium signaling regulates the biosynthesis of ethylene and polyamines, which are involved in plant growth and development as well as various stress responses. ..
  26. Lee J, Rhee B, Bae G, Han Y, Kim J. Stimulation of Oct-4 activity by Ewing's sarcoma protein. Stem Cells. 2005;23:738-51 pubmed
    ..Taken together, our results suggest that Oct-4-mediated transactivation is stimulated by EWS. ..
  27. Kim S, Hwang S, Chung I, Lee J. Sequence-specific binding to telomeric DNA by CEH-37, a homeodomain protein in the nematode Caenorhabditis elegans. J Biol Chem. 2003;278:28038-44 pubmed
    ..The identification of CEH-37 as a telomere-binding protein may represent an evolutionary conservation of telomere-binding proteins in terms of tertiary protein structure rather than primary amino acid sequence. ..
  28. Kim W, Park E, Lee H, Seong R, Park S. Physical interaction between recombinational proteins Rhp51 and Rad22 in Schizosaccharomyces pombe. J Biol Chem. 2002;277:30264-70 pubmed
    ..These results suggest that the interaction between Rhp51 and Rad22 is crucial for the recombinational repair function of Rhp51. However, the significance of this interaction on the function of Rad22 remains to be characterized further. ..
  29. Kang J, Kim M, Lee J, Park H, Min Nyung L, Auh C, et al. Histone deacetylase-1 represses transcription by interacting with zinc-fingers and interfering with the DNA binding activity of Sp1. Cell Physiol Biochem. 2005;16:23-30 pubmed
  30. Choi Y, Cho S, Kim H, Kim J, Bae S, Park S. Cloning and characterization of mouse disabled 2 interacting protein 2, a mouse orthologue of human NOSTRIN. Biochem Biophys Res Commun. 2005;326:594-9 pubmed
    ..The eNos was not detected in RA-treated F9 cell. These results suggest that mDaIP2 somehow functions in a different fashion from NOSTRIN in F9 cell differentiation and that its function may be concerted with that of mDab2. ..
  31. Kim Y, Kim S, Lee M, Lee I, Park H, Seo P, et al. HD-ZIP III activity is modulated by competitive inhibitors via a feedback loop in Arabidopsis shoot apical meristem development. Plant Cell. 2008;20:920-33 pubmed publisher
    ..We therefore propose that HD-ZIP III activity in regulating SAM development is modulated by, among other things, a feedback loop involving the competitive inhibitors ZPR3 and ZPR4. ..
  32. Son O, Cho S, Kim S, Kim W. In vitro and in vivo interaction of AtRma2 E3 ubiquitin ligase and auxin binding protein 1. Biochem Biophys Res Commun. 2010;393:492-7 pubmed publisher
    ..This suggests that ABP1 is controlled by the Ub/26S proteasome system. Therefore, AtRma2 is likely involved in the cellular regulation of ABP1 expression levels. ..
  33. Kim J, Jang S, Song K. Different levels of Bfa1/Bub2 GAP activity are required to prevent mitotic exit of budding yeast depending on the type of perturbations. Mol Biol Cell. 2008;19:4328-40 pubmed publisher
    ..These findings demonstrate that there is a GAP-independent surveillance mechanism of Bfa1/Bub2, which, together with the GTP/GDP switch of Tem1, may be required for the genomic stability of cells with misaligned spindles. ..
  34. Lee K, Lee S, Kim B, Chang S, Kim S, Paick J, et al. Dazl can bind to dynein motor complex and may play a role in transport of specific mRNAs. EMBO J. 2006;25:4263-70 pubmed
    ..The Dazl-bound mRNAs may be stored at specific sites and would be available for future developmental processes. Our study revealed the presence of an active mRNA transport system in mouse male germ cells. ..
  35. Kim S, Kim J. Reduction of invasion in human fibrosarcoma cells by ribosomal protein S3 in conjunction with Nm23-H1 and ERK. Biochim Biophys Acta. 2006;1763:823-32 pubmed
    ..These results suggest that rpS3 inhibits invasion via blocking the ERK pathway and MMP-9 secretion; the results also suggest that the interaction of rpS3 and nm23-H1 appears to be critical in this inhibition. ..
  36. Kim J, Kim S, Ko J, Yim J, Park J, Park J. Interaction of pro-apoptotic protein HGTD-P with heat shock protein 90 is required for induction of mitochondrial apoptotic cascades. FEBS Lett. 2006;580:3270-5 pubmed
  37. Kang S, Han J, Park J, Skarstad K, Hwang D. SeqA protein stimulates the relaxing and decatenating activities of topoisomerase IV. J Biol Chem. 2003;278:48779-85 pubmed
  38. Kang S, Lim W, Kim K. A protein interaction map of soybean mosaic virus strain G7H based on the yeast two-hybrid system. Mol Cells. 2004;18:122-6 pubmed
    ..Although not all of the protein-protein interactions previously reported in other potyviruses were detected, the interactions revealed here were, in general, similar to those reported previously. ..
  39. Han S, Ryu J, Oh C, Nam K, Nam H, Jang I, et al. The moleskin gene product is essential for Caudal-mediated constitutive antifungal Drosomycin gene expression in Drosophila epithelia. Insect Mol Biol. 2004;13:323-7 pubmed
    ..These in vivo data suggest that DIM-7 mediates Caudal nuclear localization, which is important for the proper Caudal function necessary for regulating innate immune genes in Drosophila. ..
  40. Yun H, Kim S, Kim H, Kostenis E, Kim J, Seong J, et al. The novel cellular mechanism of human 5-HT6 receptor through an interaction with Fyn. J Biol Chem. 2007;282:5496-505 pubmed
    ..These findings suggest that Fyn may play an important role in 5-HT(6)R- mediated signaling pathways in the central nervous system. ..
  41. Seo J, Kang S, Seo B, Jung J, Kim K. Mutational analysis of interaction between coat protein and helper component-proteinase of Soybean mosaic virus involved in aphid transmission. Mol Plant Pathol. 2010;11:265-76 pubmed publisher
    ..Based on the combination of our current results with previous findings, the possibility that aphid transmission may be regulated by more complex molecular interactions than the simple involvement of HC-Pro as a bridge is discussed...
  42. Lee H, Kang S, Bae S, Choi B, Hwang D. SeqA protein aggregation is necessary for SeqA function. J Biol Chem. 2001;276:34600-6 pubmed
    ..SeqA tetramers and aggregates might be involved in the formation of SeqA foci required for the segregation of chromosomal DNA as well as the regulation of chromosomal initiation. ..
  43. Jeong J, Rhee D, Cho S, Hae K, Kim D, Won M, et al. Cloning and characterization of the kinesin-related protein, Krp1p, in Schizosaccharomyces pombe. Mol Cells. 2002;13:389-98 pubmed
    ..AF351206). The data from the overexpression and two-hybrid study of Krplp may provide information that Krplp can have roles in cytokinesis with myosin. ..
  44. Park Y, Yoon S, Yoon J. TRIP12 functions as an E3 ubiquitin ligase of APP-BP1. Biochem Biophys Res Commun. 2008;374:294-8 pubmed publisher
    ..Our data suggest that that TRIP12 promotes degradation of APP-BP1 by catalyzing its ubiquitination, which in turn modulates the neddylation pathway. ..
  45. Lee K, Kwak Y, Kim D, Chang M, Lee Y, Lee Y. Human zinc finger protein 161, a novel transcriptional activator of the dopamine transporter. Biochem Biophys Res Commun. 2004;313:969-76 pubmed
    ..Both the HY1 sequence and the downstream region were necessary for activation of the hDAT promoter by ZFP161. This finding suggests that the site of cofactor interaction with ZFP161 may exist downstream of HY1. ..
  46. Kim T, Kim S, Yun H, Chung K, Han Y, Shin H, et al. Modulation of Ca(v)3.1 T-type Ca2+ channels by the ran binding protein RanBPM. Biochem Biophys Res Commun. 2009;378:15-20 pubmed publisher
    ..1 currents, an effect that was abolished by the expression of RanBPM. These results suggest that RanBPM could be a key regulator of Ca(v)3.1 channel-mediated signaling pathways. ..
  47. Lee E, Jo M, Rho S, Park K, Yoo Y, Park J, et al. Dkk3, downregulated in cervical cancer, functions as a negative regulator of beta-catenin. Int J Cancer. 2009;124:287-97 pubmed publisher
    ..We conclude from our finding that Dkk3 is a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway. ..
  48. Han S, Park D, Park S, Hong S. Identification of Rab6 as an N-ethylmaleimide-sensitive fusion protein-binding protein. Biochem J. 2000;352 Pt 1:165-73 pubmed
    ..Taken together, we propose a model in which Rab can form an ATP hydrolysis-regulated complex with NSF, and function as a signalling molecule to deliver the signal of vesicle fusion through the interaction with NSF. ..
  49. Choi I, Jeong M, Ham M, Sung H, Yun C. Novel Ree1 regulates the expression of ENO1 via the Snf1 complex pathway in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2008;377:395-399 pubmed publisher
    ..Taken together, these results suggest that Ree1p functions in the galactose metabolic pathway via the Gal83 protein and that it may control the level of Eno1p, which is also affected by the Snf1 complex, in S. cerevisiae. ..
  50. Kim J, Sim S, Yong T, Park S. Interaction of BOP1, a protein for ribosome biogenesis, with EB1 in Giardia lamblia. Parasitol Res. 2008;103:1459-64 pubmed publisher
    ..lamblia was confirmed by co-immunoprecipitation of in vitro-synthesized Bop1 and EB1 proteins. This result suggests coordination between ribosome biogenesis and cell cycle-related processes such as mitosis...
  51. Cho S, Ryu M, Song C, Kwak J, Kim W. Arabidopsis PUB22 and PUB23 are homologous U-Box E3 ubiquitin ligases that play combinatory roles in response to drought stress. Plant Cell. 2008;20:1899-914 pubmed publisher
    ..Overall, our results suggest that PUB22 and PUB23 coordinately control a drought signaling pathway by ubiquitinating cytosolic RPN12a in Arabidopsis. ..
  52. Lee I, Kim K, Kim H, Lee J, Ryu C, Chun H, et al. MAP, a protein interacting with a tumor suppressor, merlin, through the run domain. Biochem Biophys Res Commun. 2004;325:774-83 pubmed
    ..In addition, merlin and MAP synergistically reduced the colony formation of NIH3T3 cells. These results suggest that MAP may play a cooperative role in the merlin-mediated growth suppression of cells. ..
  53. Kim C, Cho Y, Kang C, Kim M, Lee H, Cho E, et al. Filamin is essential for shedding of the transmembrane serine protease, epithin. EMBO Rep. 2005;6:1045-51 pubmed
    ..These results show that filamin has an essential role in shedding by linking epithin to the as yet unidentified metalloprotease-shedding enzyme(s). ..
  54. Kang J, Kim T, Ko Y, Rho S, Park S, Kim M, et al. Heat shock protein 90 mediates protein-protein interactions between human aminoacyl-tRNA synthetases. J Biol Chem. 2000;275:31682-8 pubmed
    ..Thus, hsp90 appears to mediate protein-protein interactions of mammalian tRNA synthetases. ..
  55. Jang H, Yoon K, Shin Y, Kim J, Lee S. PIAS3 suppresses NF-kappaB-mediated transcription by interacting with the p65/RelA subunit. J Biol Chem. 2004;279:24873-80 pubmed
    ..Taken together, these data suggest that PIAS3 may function in vivo as a modulator in suppressing the transcriptional activity of p65. ..
  56. Um J, Min D, Rhim H, Kim J, Paik S, Chung K. Parkin ubiquitinates and promotes the degradation of RanBP2. J Biol Chem. 2006;281:3595-603 pubmed
    ..Our findings suggested that the intracellular levels of RanBP2 and its functional activity may be modulated by Parkin-mediated ubiquitination and proteasomal pathways. ..
  57. Ha S, Shin S, Lee Y, Kim S, Kim H, Namkoong H, et al. HCCRBP-1 directly interacting with HCCR-1 induces tumorigenesis through P53 stabilization. Int J Cancer. 2008;122:501-8 pubmed
    ..Indeed, HCCRBP-1 decreased p21 promoter activity probably via p53 stabilization leading to the defective function. These results indicate that HCCRBP-1 oncogene induces p53 stabilization and thereby contributes to tumorigenesis. ..
  58. Kim M, Kang M, Nam H, Bae Y, Kim Y, Chung I. Regulation of telomeric repeat binding factor 1 binding to telomeres by casein kinase 2-mediated phosphorylation. J Biol Chem. 2008;283:14144-52 pubmed publisher
    ..Our findings suggest that CK2-mediated phosphorylation of TRF1 plays an important role in modulating telomere length homeostasis by determining the levels of TRF1 at telomeres. ..
  59. Kim E, Park J, Um S. Identification of Daxx interacting with p73, one of the p53 family, and its regulation of p53 activity by competitive interaction with PML. Nucleic Acids Res. 2003;31:5356-67 pubmed
    ..Therefore, Daxx is implicated in the regulation of the cell cycle and apoptosis through transcriptional regulation of p53 and possibly its family members. ..
  60. Lee Y, Lee S, Sin H, Kim E, Um S. Kinase activity-independent suppression of p73alpha by AMP-activated kinase alpha (AMPKalpha). Oncogene. 2009;28:1040-52 pubmed publisher
    ..From these data, we speculate that AMPKalpha may provide a molecular clue to understand the repressive role of the C-terminus of p73alpha in transcription and DNA damage response. ..
  61. Oh H, Kwon H, Sun S, Yang C. QM, a putative tumor suppressor, regulates proto-oncogene c-yes. J Biol Chem. 2002;277:36489-98 pubmed
    ..These results suggest that the QM protein might be a regulator for various signal transduction pathways involving SH3 domain-containing membrane proteins. ..
  62. Rho S, Park Y, Park K, Lee S, Lee J. A novel cervical cancer suppressor 3 (CCS-3) interacts with the BTB domain of PLZF and inhibits the cell growth by inducing apoptosis. FEBS Lett. 2006;580:4073-80 pubmed
    ..These combined results suggest that the potential tumor suppressor activity of CCS-3 may be mediated by its interaction with PLZF. ..
  63. Lee J, Kim H, Ryu C, Kim J, Choi B, Lim Y, et al. Merlin, a tumor suppressor, interacts with transactivation-responsive RNA-binding protein and inhibits its oncogenic activity. J Biol Chem. 2004;279:30265-73 pubmed
    ..These results provide the first clue to the functional interaction between TRBP and merlin and suggest a novel mechanism for the tumor suppressor function of merlin both in vitro and in vivo. ..
  64. Sihn C, Suh E, Lee K, Kim T, Kim S. p55CDC/hCDC20 mutant induces mitotic catastrophe by inhibiting the MAD2-dependent spindle checkpoint activity in tumor cells. Cancer Lett. 2003;201:203-10 pubmed
    ..Taken together, these results suggest that agents blocking the spindle checkpoint response may induce tumor cells to become more sensitive to spindle poison drugs, providing a powerful tool to improve chemotherapy. ..
  65. Park E, Choi Y, Ahn E, Park I, Yun Y. The adaptor protein LAD/TSAd mediates laminin-dependent T cell migration via association with the 67 kDa laminin binding protein. Exp Mol Med. 2009;41:728-36 pubmed publisher
    ..1 cells overexpressing dominant negative form of TSAd or TSAd shRNA. These data show that, upon TCR plus integrin costimulation, TSAd associates with LBP and mediates T lymphocyte migration. ..
  66. Ko J, Jang S, Kim Y, Kim I, Sung H, Kim H, et al. Human LZIP binds to CCR1 and differentially affects the chemotactic activities of CCR1-dependent chemokines. FASEB J. 2004;18:890-2 pubmed
  67. Lee S, Choi J, Sung Y, Park H, Rhim H, Kang S. E3 ligase activity of RING finger proteins that interact with Hip-2, a human ubiquitin-conjugating enzyme. FEBS Lett. 2001;503:61-4 pubmed
    ..RNF2 showed a ubiquitin ligase (E3) activity in the presence of Hip-2, suggesting that a subset of RING finger proteins may have roles as E3s. ..
  68. Jeong Y, Lee J, Kim K, Yoo J, Rhee K. Characterization of NIP2/centrobin, a novel substrate of Nek2, and its potential role in microtubule stabilization. J Cell Sci. 2007;120:2106-16 pubmed
    ..Based on our results, we propose that NIP2 has a role in stabilizing the microtubule structure. Phosphorylation may be crucial for mobilization of the protein to a new microtubule and stabilizing it. ..
  69. Song K, Jung D, Jung Y, Lee S, Lee I. Interaction of human Ku70 with TRF2. FEBS Lett. 2000;481:81-5 pubmed
    ..This interaction was confirmed using bacterial fusion proteins and co-immunoprecipitations from eukaryotic cells overexpressing TRF2. The transfected TFR2 colocalized with Ku70. ..
  70. Kim J, Han W, Namkung W, Lee J, Kim K, Shin H, et al. Inhibitory regulation of cystic fibrosis transmembrane conductance regulator anion-transporting activities by Shank2. J Biol Chem. 2004;279:10389-96 pubmed
    ..Our results indicate that Shank2 negatively regulates CFTR and that this may play a significant role in maintaining epithelial homeostasis under normal and diseased conditions such as those presented by secretory diarrhea. ..
  71. Kim J, Lee H, Kim Y, Yoo S, Park E, Park S. The SAM domains of Anks family proteins are critically involved in modulating the degradation of EphA receptors. Mol Cell Biol. 2010;30:1582-92 pubmed publisher
    ..Taken together, our studies suggest that the SAM domains of Anks family proteins play a pivotal role in enhancing the stability of EphA receptors by modulating the ubiquitination process. ..
  72. Kim D, Hesketh A, Kim E, Song J, Lee D, Kim I, et al. Complex extracellular interactions of proteases and a protease inhibitor influence multicellular development of Streptomyces coelicolor. Mol Microbiol. 2008;70:1180-93 pubmed publisher
    ..A model is presented that links intracellular regulatory events with an extracellular protease cascade to facilitate normal development...
  73. Park B, Eo H, Jang I, Kang H, Song J, Seo H. Ubiquitination of LHY by SINAT5 regulates flowering time and is inhibited by DET1. Biochem Biophys Res Commun. 2010;398:242-6 pubmed publisher
    ..Interestingly, LHY ubiquitination by SINAT5 was inhibited by DET1. Late flowering of sinat5 mutants indicates that flowering time can be controlled by DET1 through regulation of LHY stability by SINAT5. ..
  74. Park H, Jung H, Choi K, Baik J, Rhim H. Direct interaction and functional coupling between voltage-gated CaV1.3 Ca2+ channel and GABAB receptor subunit 2. FEBS Lett. 2010;584:3317-22 pubmed publisher
    ..3 currents and intracellular Ca2+ via CaV1.3, but not CaV1.2. These results show a physical and functional interaction between CaV1.3 and GABABR, suggesting the potential pivotal roles of CaV1.3 in the CNS. ..