Experts and Doctors on protein denaturation in Seoul, South Korea


Locale: Seoul, South Korea
Topic: protein denaturation

Top Publications

  1. Heo Y, Kim S, Kim E, Lee K. Quaternary structural analysis of nucleoside diphosphate kinases using capillary electrophoresis. J Chromatogr A. 1997;781:251-61 pubmed
    ..The structural information of NDP kinase obtained by analysis using capillary electrophoresis is valuable for understanding its suggested biological function as a tumor metastasis suppressor and c-myc transcription factor, etc. ..
  2. Oh K, Jung Y, Hwang G, Cho M. Conformational distributions of denatured and unstructured proteins are similar to those of 20 × 20 blocked dipeptides. J Biomol NMR. 2012;53:25-41 pubmed publisher
  3. Lee D, Kim K, Yu Y, Kim K. Substitution of aspartic acid with glutamic acid increases the unfolding transition temperature of a protein. Biochem Biophys Res Commun. 2004;320:900-6 pubmed
    ..5 degrees C. The change of Tm destabilizes the MjTRX by 0.55 kcal/mol and stabilizes the ETRX by 0.45 kcal/mol in free energy. ..
  4. Pack S, Yoo Y. Protein thermostability: structure-based difference of amino acid between thermophilic and mesophilic proteins. J Biotechnol. 2004;111:269-77 pubmed
    ..could be critical factors related with protein thermostability. ..
  5. Rhee J, Kim D, Ahn D, Yun J, Jang S, Shin H, et al. Analysis of the thermostability determinants of hyperthermophilic esterase EstE1 based on its predicted three-dimensional structure. Appl Environ Microbiol. 2006;72:3021-5 pubmed
    ..Our results verified the predicted 3D structure of EstE1 and identified the ion pair networks and hydrophobic interactions that are critical determinants for the thermostability of EstE1. ..
  6. Suh J, Lee Y, Lee H, Kang M, Hur Y, Lee S, et al. Dispersive liquid-liquid microextraction based on solidification of floating organic droplets followed by high performance liquid chromatography for the determination of duloxetine in human plasma. J Pharm Biomed Anal. 2013;75:214-9 pubmed publisher
  7. Chung J, Back J, Park Y, Han Y. Biochemical characterization of a novel hypoxanthine/xanthine dNTP pyrophosphatase from Methanococcus jannaschii. Nucleic Acids Res. 2001;29:3099-107 pubmed
    ..This report is the first identification and functional characterization of an enzyme hydrolyzing non-canonical nucleotides, dITP and XTP...
  8. Kim J, Kim B, Lopez Ferrer D, Petritis K, Smith R. Nanobiocatalysis for protein digestion in proteomic analysis. Proteomics. 2010;10:687-99 pubmed publisher
  9. Ahn K, Park J, Han K, Song J, Lee J. YrhB is a highly stable small protein with unique chaperone-like activity in Escherichia coli BL21(DE3). FEBS Lett. 2012;586:1044-8 pubmed publisher
    ..As a cis-acting fusion partner, YrhB also significantly reduced inclusion body formation of nine aggregation-prone heterologous proteins in BL21(DE3). Unlike conventional small HSPs, YrhB remained monomer under heat shock condition. ..

More Information


  1. Seo E, Lee C, Yu M. Concerted regulation of inhibitory activity of alpha 1-antitrypsin by the native strain distributed throughout the molecule. J Biol Chem. 2002;277:14216-20 pubmed
    ..The results suggest that the native strain of alpha(1)-antitrypsin distributed throughout the molecule regulates the inhibitory function in a concerted manner. ..
  2. Lee S, Kwon H, Kim Y, Lee K, Kim M, Yoon B. Effects of hsp70.1 gene knockout on the mitochondrial apoptotic pathway after focal cerebral ischemia. Stroke. 2004;35:2195-9 pubmed
    ..These findings suggest that the deletion of the hsp70.1 gene increases cytochrome c release into the cytoplasm and subsequent caspase-3 activation, thereby exacerbating apoptosis after focal cerebral ischemia. ..
  3. Han K, Park J, Seo H, Ahn K, Lee J. Multiple stressor-induced proteome responses of Escherichia coli BL21(DE3). J Proteome Res. 2008;7:1891-903 pubmed publisher
    ..The quantitative and systematic proteome analyses that we have performed provide more detailed information on E. coli BL21(DE3), a widely used host strain for recombinant protein overexpression. ..
  4. Lee K, Hong T, Hahn J. Roles of 17-AAG-induced molecular chaperones and Rma1 E3 ubiquitin ligase in folding and degradation of Pendrin. FEBS Lett. 2012;586:2535-41 pubmed publisher
    ..Furthermore, we demonstrate that Rma1, an E3 ubiquitin ligase localized in the ER membrane, is involved in Pendrin degradation. ..