Experts and Doctors on saccharomyces cerevisiae in Singapore, Central Singapore, Singapore


Locale: Singapore, Central Singapore, Singapore
Topic: saccharomyces cerevisiae

Top Publications

  1. Xie W, Kanehara K, Sayeed A, Ng D. Intrinsic conformational determinants signal protein misfolding to the Hrd1/Htm1 endoplasmic reticulum-associated degradation system. Mol Biol Cell. 2009;20:3317-29 pubmed publisher
    ..Only if both elements are present will the substrate be targeted to the ERAD pathway for degradation. ..
  2. Jiang S, Ramachandran S. Comparative and evolutionary analysis of genes encoding small GTPases and their activating proteins in eukaryotic genomes. Physiol Genomics. 2006;24:235-51 pubmed
  3. Goh P, Lim H, Surana U. Cdc20 protein contains a destruction-box but, unlike Clb2, its proteolysisis not acutely dependent on the activity of anaphase-promoting complex. Eur J Biochem. 2000;267:434-49 pubmed
    ..This suggests the existence of proteolytic mechanisms in G1 that can degrade destruction-box containing proteins, such as Cdc20, in an APC-independent manner. ..
  4. Khong J, Zhang T, Gunaratne J, Blackstock W, Surana U. "Reductional anaphase" in replication-defective cells is caused by ubiquitin-conjugating enzyme Cdc34-mediated deregulation of the spindle. Cell Cycle. 2012;11:2896-910 pubmed publisher
    ..This regulatory circuit governs spindle behavior in the early part of the division cycle and precipitates catastrophic chromosome segregation in the absence of DNA replication. ..
  5. Shui G, Guan X, Low C, Chua G, Goh J, Yang H, et al. Toward one step analysis of cellular lipidomes using liquid chromatography coupled with mass spectrometry: application to Saccharomyces cerevisiae and Schizosaccharomyces pombe lipidomics. Mol Biosyst. 2010;6:1008-17 pubmed publisher
    ..We further discussed dynamic changes of phospholipids, sphingolipids and neutral lipids in the progress of programmed cell death in Deltaplh1Deltadga1 cells of S. pombe. ..
  6. Shi S, Liang Y, Zhang M, Ang E, Zhao H. A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae. Metab Eng. 2016;33:19-27 pubmed publisher
    ..The simplicity and high efficiency of the Di-CRISPR platform could provide a superior alternative to high copy plasmids and would render this platform an invaluable tool for genome editing and metabolic engineering in S. cerevisiae. ..
  7. Chai X, Nagarajan S, Kim K, Lee K, Choi J. Regulation of the boundaries of accessible chromatin. PLoS Genet. 2013;9:e1003778 pubmed publisher
  8. Thaker Y, Hunke C, Yau Y, Shochat S, Li Y, Gruber G. Association of the eukaryotic V1VO ATPase subunits a with d and d with A. FEBS Lett. 2009;583:1090-5 pubmed publisher
    ..The data are discussed in light of subunit a and d forming a peripheral stalk, connecting the catalytic A(3)B(3) hexamer with V(O). ..
  9. Wang P, Zhang Y, Li H, Chieu H, Munn A, Yang H. AAA ATPases regulate membrane association of yeast oxysterol binding proteins and sterol metabolism. EMBO J. 2005;24:2989-99 pubmed
    ..Our data suggest that a common mechanism may exist for AAA proteins to regulate the membrane association of yeast OSBP proteins and that these two protein families may function together to control subcellular lipid transport. ..

More Information


  1. Cheng Z, Liu Y, Wang C, Parker R, Song H. Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination. Protein Sci. 2004;13:2673-84 pubmed
    ..The functional implications for Ski8p function in both mRNA decay and meiotic recombination are discussed. ..
  2. Shui G, Guan X, Gopalakrishnan P, Xue Y, Goh J, Yang H, et al. Characterization of substrate preference for Slc1p and Cst26p in Saccharomyces cerevisiae using lipidomic approaches and an LPAAT activity assay. PLoS ONE. 2010;5:e11956 pubmed publisher
    ..Our current findings expand the enzymes and existing scope of acyl-CoA donors for glycerophospholipid biosynthesis. ..
  3. Lim M, Siew W, Zhao J, Tay Y, Ang E, Lehming N. Galactose induction of the GAL1 gene requires conditional degradation of the Mig2 repressor. Biochem J. 2011;435:641-9 pubmed publisher
  4. Qiu W, Neo S, Yu X, Cai M. A novel septin-associated protein, Syp1p, is required for normal cell cycle-dependent septin cytoskeleton dynamics in yeast. Genetics. 2008;180:1445-57 pubmed publisher
    ..These results suggest that Syp1p is involved in the regulation of cell cycle-dependent dynamics of the septin cytoskeleton in yeast. ..
  5. Ismail N, Ng D. Have you HRD? Understanding ERAD is DOAble!. Cell. 2006;126:237-9 pubmed
    ..2006) and Carvalho et al. (2006) report that two distinct protein complexes at the endoplasmic reticulum membrane are responsible for the recognition and degradation of specific subsets of protein substrates. ..
  6. Bai C, Xu X, Chan F, Lee R, Wang Y. MNN5 encodes an iron-regulated alpha-1,2-mannosyltransferase important for protein glycosylation, cell wall integrity, morphogenesis, and virulence in Candida albicans. Eukaryot Cell. 2006;5:238-47 pubmed
    ..Of particular interest was the observed role of iron in regulating the enzymatic activity, suggesting an underlying relationship between Mnn5 activity and cellular iron homeostasis. ..
  7. Liang H, Lim H, Venkitaraman A, Surana U. Cdk1 promotes kinetochore bi-orientation and regulates Cdc20 expression during recovery from spindle checkpoint arrest. EMBO J. 2012;31:403-16 pubmed publisher
    ..Our findings provide fresh insights into the regulation of mitosis and have implications for the therapeutic efficacy of anti-mitotic drugs. ..
  8. Thaker Y, Roessle M, Gruber G. The boxing glove shape of subunit d of the yeast V-ATPase in solution and the importance of disulfide formation for folding of this protein. J Bioenerg Biomembr. 2007;39:275-89 pubmed
    ..Cysteins, involved in disulfide bridges, were analyzed by MALDI-TOF mass spectrometry. Finally, the solution structure of subunit d will be discussed in terms of the topological arrangement of the V(1)V(O) ATPase. ..
  9. Ong L, Lin P, Zhang X, Chia S, Yu H. Kinectin-dependent assembly of translation elongation factor-1 complex on endoplasmic reticulum regulates protein synthesis. J Biol Chem. 2006;281:33621-34 pubmed
    ..These findings suggest that anchoring the elongation factor-1 complex onto endoplasmic reticulum via EF-1delta/kinectin interaction is important for regulating protein synthesis in eukaryotic cells. ..
  10. Guan X, Souza C, Pichler H, Dewhurst G, Schaad O, Kajiwara K, et al. Functional interactions between sphingolipids and sterols in biological membranes regulating cell physiology. Mol Biol Cell. 2009;20:2083-95 pubmed publisher
  11. Teh C, Lam K, Loh C, Loo J, Yan T, Lim T. Neuronal PAS domain protein 1 is a transcriptional repressor and requires arylhydrocarbon nuclear translocator for its nuclear localization. J Biol Chem. 2006;281:34617-29 pubmed
  12. Tesker M, Selamat S, Beenstock J, Hayouka R, Livnah O, Engelberg D. Tighter ?C-helix-?L16-helix interactions seem to make p38? less prone to activation by autophosphorylation than Hog1. Biosci Rep. 2016;36: pubmed publisher
    ..The suppressors were kept wobbly, probably to allow activation by induced autophosphorylation, but became stricter in mammalian p38s than in the yeast Hog1. ..
  13. Zeng G, Cai M. Regulation of the actin cytoskeleton organization in yeast by a novel serine/threonine kinase Prk1p. J Cell Biol. 1999;144:71-82 pubmed
    ..Based on these findings, we propose that the Prk1 protein kinase regulates the actin cytoskeleton organization by modulating the activities of some actin cytoskeleton-related proteins such as Pan1p/End3p. ..
  14. Liu S, Tsao M. Inhibition of spoilage yeasts in cheese by killer yeast Williopsis saturnus var. saturnus. Int J Food Microbiol. 2009;131:280-2 pubmed publisher
    ..This study indicated that W. saturnus var. saturnus could be an effective biopreservative for cheese spoilage control. ..
  15. Xu C, Wang S, Thibault G, Ng D. Futile protein folding cycles in the ER are terminated by the unfolded protein O-mannosylation pathway. Science. 2013;340:978-81 pubmed publisher
    ..In an in vitro protein refolding assay, the modification intrinsically and irreversibly disabled the folding potential of the substrate. Thus, protein folding termination can involve a covalent glycosylation event. ..
  16. Tang X, Feng H, Chen W. Metabolic engineering for enhanced fatty acids synthesis in Saccharomyces cerevisiae. Metab Eng. 2013;16:95-102 pubmed publisher
    ..The increased fatty acids from our study may well be potential substrates for the production of hydrocarbon molecules as potential biofuels. ..
  17. Wachtler V, Balasubramanian M. Yeast lipid rafts?--an emerging view. Trends Cell Biol. 2006;16:1-4 pubmed
    ..Here, we attempt to synthesize our understanding of the function and organization of these domains from the study of fungi and identify some outstanding issues. ..
  18. Srihari S, Leong H. Employing functional interactions for characterisation and detection of sparse complexes from yeast PPI networks. Int J Bioinform Res Appl. 2012;8:286-304 pubmed publisher
    ..We demonstrate that our approach is effective in reconstructing significantly many complexes missed previously (104 recovered out of the 123 known complexes or ~47% improvement). ..
  19. Zhou H, Wong L. Comparative analysis and assessment of M. tuberculosis H37Rv protein-protein interaction datasets. BMC Genomics. 2011;12 Suppl 3:S20 pubmed publisher
    ..The results and conclusions from this comparative analysis provide valuable guidance in using these M. tuberculosis H37Rv PPI datasets in subsequent studies for a wide range of purposes. ..
  20. Chai C, Teh E, Yeong F. Unrestrained spindle elongation during recovery from spindle checkpoint activation in cdc15-2 cells results in mis-segregation of chromosomes. Mol Biol Cell. 2010;21:2384-98 pubmed publisher
    ..Our data indicate that a slower rate of transition through the cell division cycle can result in an inadequate level of Pds1p accumulation that can compromise recovery from spindle assembly checkpoint activation. ..
  21. Zhang S, Ren J, Li H, Zhang Q, Armstrong J, Munn A, et al. Ncr1p, the yeast ortholog of mammalian Niemann Pick C1 protein, is dispensable for endocytic transport. Traffic. 2004;5:1017-30 pubmed
    ..We conclude that Ncr1p does not have an essential role in known endocytic transport pathways in yeast. ..
  22. Si X, Zeng Q, Ng C, Hong W, Pallen C. Interaction of farnesylated PRL-2, a protein-tyrosine phosphatase, with the beta-subunit of geranylgeranyltransferase II. J Biol Chem. 2001;276:32875-82 pubmed
    ..Together, these results indicate that the binding of alphaGGT II and PRL-2 to betaGGT II is mutually exclusive, and suggest that PRL-2 may function as a regulator of GGT II activity. ..
  23. Wu M, Li X, Chua H, Kwoh C, Ng S. Integrating diverse biological and computational sources for reliable protein-protein interactions. BMC Bioinformatics. 2010;11 Suppl 7:S8 pubmed publisher
    ..Our proposed method also performed significantly better than merely using each of individual evidence sources, illustrating the importance of integrating various biological and computational sources of data and evidence. ..
  24. Wang S, Ng D. Evasion of endoplasmic reticulum surveillance makes Wsc1p an obligate substrate of Golgi quality control. Mol Biol Cell. 2010;21:1153-65 pubmed publisher
    ..By evading ERAD through these passive and active mechanisms, Wsc1p is fully dependent on the post-ER system for its quality control. ..
  25. Rajmohan R, Wong M, Meng L, Munn A, Thanabalu T. Las17p-Vrp1p but not Las17p-Arp2/3 interaction is important for actin patch polarization in yeast. Biochim Biophys Acta. 2009;1793:825-35 pubmed publisher
    ..Using these mutant and truncated forms of yeast WASP we provide novel evidence that WASP interaction with WIP is more important than interaction with Arp2/3 for polarized actin assembly and endocytosis in yeast. ..
  26. Wang J, Neo S, Cai M. Regulation of the yeast formin Bni1p by the actin-regulating kinase Prk1p. Traffic. 2009;10:528-35 pubmed publisher
    ..This finding extends the roles of Prk1p in the regulation of actin dynamics to be associated with both anterograde and retrograde transport pathways, i.e. exocytosis and endocytosis, in yeast. ..
  27. Zeng G, Yu X, Cai M. Regulation of yeast actin cytoskeleton-regulatory complex Pan1p/Sla1p/End3p by serine/threonine kinase Prk1p. Mol Biol Cell. 2001;12:3759-72 pubmed
    ..These results lend a strong support to the model that the phosphorylation of the Pan1p/Sla1p/End3p complex by Prk1p is one of the important mechanisms by which the organization and functions of the actin cytoskeleton are regulated. ..
  28. Lee P, Ong Y, Yu B, Curran P, Liu S. Profile of volatile compounds during papaya juice fermentation by a mixed culture of Saccharomyces cerevisiae and Williopsis saturnus. Food Microbiol. 2010;27:853-61 pubmed publisher
    ..The study suggests that papaya juice fermentation with a mixed culture of S. cerevisiae and W. saturnus may be able to result in the formation of more complex aroma compounds and higher ethanol level than those using single yeasts...
  29. Huang B, Zeng G, Ng A, Cai M. Identification of novel recognition motifs and regulatory targets for the yeast actin-regulating kinase Prk1p. Mol Biol Cell. 2003;14:4871-84 pubmed
    ..These results allowed us to conclude that Scd5p is likely another regulatory target of Prk1p. ..
  30. Thibault G, Shui G, Kim W, McAlister G, Ismail N, Gygi S, et al. The membrane stress response buffers lethal effects of lipid disequilibrium by reprogramming the protein homeostasis network. Mol Cell. 2012;48:16-27 pubmed publisher
    ..We demonstrate that the UPR maintains protein biogenesis, quality control, and membrane integrity-functions otherwise lethally compromised in lipid dysregulated cells. ..
  31. Chen C, Zheng B, Han J, Lin S. Characterization of a novel mammalian RGS protein that binds to Galpha proteins and inhibits pheromone signaling in yeast. J Biol Chem. 1997;272:8679-85 pubmed
  32. Lim H, Surana U. Cdc20, a beta-transducin homologue, links RAD9-mediated G2/M checkpoint control to mitosis in Saccharomyces cerevisiae. Mol Gen Genet. 1996;253:138-48 pubmed
    ..Therefore, we suggest that the DNA damage-induced checkpoint controls prevent mitosis by inhibiting the nuclear division pathway requiring CDC20 function. ..
  33. Coe J, Lim A, Xu J, Hong W. A role for Tlg1p in the transport of proteins within the Golgi apparatus of Saccharomyces cerevisiae. Mol Biol Cell. 1999;10:2407-23 pubmed
    ..Morphological analyses by electron microscopy reveal that cells depleted of Tlg1p or tlg1 ts mutants incubated at the restrictive temperature accumulate 40- to 50-nm vesicles and experience fragmentation of the vacuole. ..
  34. Crasta K, Huang P, Morgan G, Winey M, Surana U. Cdk1 regulates centrosome separation by restraining proteolysis of microtubule-associated proteins. EMBO J. 2006;25:2551-63 pubmed
  35. Xue X, Lehming N. Nhp6p and Med3p regulate gene expression by controlling the local subunit composition of RNA polymerase II. J Mol Biol. 2008;379:212-30 pubmed publisher
    ..Our results further suggest that Rpb4p inhibits transcription initiation but stimulates transcription elongation and that Nhp6p and Med3p regulate gene expression by controlling the local subunit composition of RNA polymerase II. ..
  36. Krishnan V, Nirantar S, Crasta K, Cheng A, Surana U. DNA replication checkpoint prevents precocious chromosome segregation by regulating spindle behavior. Mol Cell. 2004;16:687-700 pubmed
    ..Thus, the DNA replication checkpoint prevents precocious chromosome segregation, not by inhibiting entry into mitosis as widely believed, but by directly regulating spindle dynamics. ..
  37. Tang H, Munn A, Cai M. EH domain proteins Pan1p and End3p are components of a complex that plays a dual role in organization of the cortical actin cytoskeleton and endocytosis in Saccharomyces cerevisiae. Mol Cell Biol. 1997;17:4294-304 pubmed
  38. Bongards C, Chew B, Lehming N. The TATA-binding protein is not an essential target of the transcriptional activators Gal4p and Gcn4p in Saccharomyces cerevisiae. Biochem J. 2003;370:141-7 pubmed
    ..Furthermore, detailed analysis of a Tbp1 mutant deficient for transcriptional activation by Gal4p revealed that the mutant is defective in interactions with five other proteins involved in the process of transcription. ..
  39. Jin M, Cai M. A novel function of Arp2p in mediating Prk1p-specific regulation of actin and endocytosis in yeast. Mol Biol Cell. 2008;19:297-307 pubmed
  40. Goh P, Surana U. Cdc4, a protein required for the onset of S phase, serves an essential function during G(2)/M transition in Saccharomyces cerevisiae. Mol Cell Biol. 1999;19:5512-22 pubmed
    ..Collectively, these observations suggest that, in addition to its involvement in the initiation of S phase, Cdc4 may also be required for the onset of anaphase. ..
  41. Rishikesan S, Gruber G. Structural elements of the C-terminal domain of subunit E (E???????) from the Saccharomyces cerevisiae V?V? ATPase determined by solution NMR spectroscopy. J Bioenerg Biomembr. 2011;43:447-55 pubmed publisher
    ..These new structural details of subunit E are discussed in the light of the structural arrangements of this subunit inside the V?- and V?V? ATPase. ..
  42. Chin C, Bennett A, Ma W, Hall M, Yeong F. Dependence of Chs2 ER export on dephosphorylation by cytoplasmic Cdc14 ensures that septum formation follows mitosis. Mol Biol Cell. 2012;23:45-58 pubmed publisher
    ..Thus, by requiring declining Cdk1 activity and Cdc14 nuclear release for Chs2 ER export, cells ensure that septum formation is contingent upon chromosome separation and exit from mitosis. ..
  43. Prasad R, Kawaguchi S, Ng D. A nucleus-based quality control mechanism for cytosolic proteins. Mol Biol Cell. 2010;21:2117-27 pubmed publisher
    ..Instead, the Hsp70 chaperone system is needed for efficient import and degradation. These data reveal a new function of the nucleus as a compartment central to the quality control of cytosolic proteins. ..
  44. Li B, Cong F, Tan C, Wang S, Goff S. Aph2, a protein with a zf-DHHC motif, interacts with c-Abl and has pro-apoptotic activity. J Biol Chem. 2002;277:28870-6 pubmed
    ..These results suggest that Aph2 may be involved in ER stress-induced apoptosis in which c-Abl plays an important role. ..
  45. Basak S, Balakrishna A, Manimekalai M, Gruber G. Crystallization and preliminary X-ray crystallographic analysis of subunit F (F(1-94)), an essential coupling subunit of the eukaryotic V(1)V(O)-ATPase from Saccharomyces cerevisiae. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012;68:1055-9 pubmed publisher
    ..3 Å and belonged to space group C222(1), with unit-cell parameters a = 47.22, b = 160.83, c = 102.74 Å. Initial phasing and model building suggested the presence of four molecules in the asymmetric unit. ..
  46. Basak S, Lim J, Manimekalai M, Balakrishna A, Gruber G. Crystal and NMR structures give insights into the role and dynamics of subunit F of the eukaryotic V-ATPase from Saccharomyces cerevisiae. J Biol Chem. 2013;288:11930-9 pubmed publisher
  47. Lim C, Luhe A, Jingying C, Balagurunathan B, Wu J, Zhao H. Size of gene specific inverted repeat--dependent gene deletion In Saccharomyces cerevisiae. PLoS ONE. 2013;8:e72137 pubmed publisher
    ..Finally, the use of gene-specific inverted repeats of target genes enables simultaneous gene deletions. The procedure has the potential for application on other yeast strains to achieve precise and efficient removal of gene sequences. ..
  48. Zhu J, Deng S, Lu P, Bu W, Li T, Yu L, et al. The Ccl1-Kin28 kinase complex regulates autophagy under nitrogen starvation. J Cell Sci. 2016;129:135-44 pubmed publisher
    ..The identification of this previously unknown regulatory pathway sheds new light on the complex signaling network that governs autophagy activity. ..
  49. Estoppey D, Lee C, Janoschke M, Lee B, Wan K, Dong H, et al. The Natural Product Cavinafungin Selectively Interferes with Zika and Dengue Virus Replication by Inhibition of the Host Signal Peptidase. Cell Rep. 2017;19:451-460 pubmed publisher
    ..This study provides an effective compound against the eukaryotic signal peptidase and independent confirmation of the recently identified critical role of the signal peptidase in the replicative cycle of flaviviruses. ..
  50. Fei W, Shui G, Gaeta B, Du X, Kuerschner L, Li P, et al. Fld1p, a functional homologue of human seipin, regulates the size of lipid droplets in yeast. J Cell Biol. 2008;180:473-82 pubmed publisher
    ..These results suggest that an evolutionally conserved function of seipin in phospholipid metabolism and LD formation may be functionally important in human adipogenesis. ..
  51. Zhang T, Nirantar S, Lim H, Sinha I, Surana U. DNA damage checkpoint maintains CDH1 in an active state to inhibit anaphase progression. Dev Cell. 2009;17:541-51 pubmed publisher
    ..Hence, the DNA damage checkpoint suppresses both cohesin cleavage and spindle elongation to preserve chromosome stability. ..
  52. Lin C, Yuan Y. Structural insights into histone H3 lysine 56 acetylation by Rtt109. Structure. 2008;16:1503-10 pubmed publisher
    ..We have further proposed the unique H3-K56 anchoring pocket and the potential H3alphaN binding groove. Our work has provided structural insights to understand the acetylation mechanism of H3-K56 by Rtt109. ..
  53. Bhunia A, Domadia P, Mohanram H, Bhattacharjya S. NMR structural studies of the Ste11 SAM domain in the dodecyl phosphocholine micelle. Proteins. 2009;74:328-43 pubmed publisher
  54. Tang H, Xu J, Cai M. Pan1p, End3p, and S1a1p, three yeast proteins required for normal cortical actin cytoskeleton organization, associate with each other and play essential roles in cell wall morphogenesis. Mol Cell Biol. 2000;20:12-25 pubmed
    ..These observations suggest that the Pan1p-End3p-Sla1p complex is required not only for normal actin cytoskeleton organization but also for normal cell wall morphogenesis in yeast. ..
  55. Loh E, Hong W. The binary interacting network of the conserved oligomeric Golgi tethering complex. J Biol Chem. 2004;279:24640-8 pubmed
    ..A model for the assembly of the COG complex is presented. ..
  56. Srihari S, Leong H. Temporal dynamics of protein complexes in PPI networks: a case study using yeast cell cycle dynamics. BMC Bioinformatics. 2012;13 Suppl 17:S16 pubmed publisher
    ..We also demonstrate that these findings provide additional support and alternative explanations to findings from existing works on the dynamics in PPI networks. ..
  57. Fan J, Cheng Z, Zhang J, Noble C, Zhou Z, Song H, et al. Solution and crystal structures of mRNA exporter Dbp5p and its interaction with nucleotides. J Mol Biol. 2009;388:1-10 pubmed publisher
    ..Binding of ATP or ADP to NTD induces significant conformational rearrangement too. ..
  58. Hsu C, Prasad R, Blackman C, Ng D. Endoplasmic reticulum stress regulation of the Kar2p/BiP chaperone alleviates proteotoxicity via dual degradation pathways. Mol Biol Cell. 2012;23:630-41 pubmed publisher
    ..Surprisingly, induced BiP/Kar2p molecules are dedicated to alleviating stress. The inability to induce KAR2 under stress had no effect on its known housekeeping functions. ..
  59. Rishikesan S, Gayen S, Thaker Y, Vivekanandan S, Manimekalai M, Yau Y, et al. Assembly of subunit d (Vma6p) and G (Vma10p) and the NMR solution structure of subunit G (G(1-59)) of the Saccharomyces cerevisiae V(1)V(O) ATPase. Biochim Biophys Acta. 2009;1787:242-51 pubmed publisher
    ..Based on the recently determined NMR structure of segment E(18-38) of subunit E of yeast V-ATPase and the presently solved structure of G(1-59), both proteins have been docked and binding epitopes have been analyzed. ..
  60. Thanabalu T, Rajmohan R, Meng L, Ren G, Vajjhala P, Munn A. Verprolin function in endocytosis and actin organization. Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain. FEBS J. 2007;274:4103-25 pubmed
  61. Wang S, Thibault G, Ng D. Routing misfolded proteins through the multivesicular body (MVB) pathway protects against proteotoxicity. J Biol Chem. 2011;286:29376-87 pubmed publisher
    ..Eliminating the MVB sorting signal diverted molecules to the vacuolar limiting membrane, resulting in the generation of toxic by-products. These data demonstrate a new role of the MVB pathway in protein quality control. ..
  62. Lim H, Goh P, Surana U. Cdc20 is essential for the cyclosome-mediated proteolysis of both Pds1 and Clb2 during M phase in budding yeast. Curr Biol. 1998;8:231-4 pubmed
    ..This notion is consistent with our observations that Cdc20 is localized in the nucleus and co-immunoprecipitates with an APC component, Cdc23. ..
  63. Widiastuti H, Kim J, Selvarasu S, Karimi I, Kim H, Seo J, et al. Genome-scale modeling and in silico analysis of ethanologenic bacteria Zymomonas mobilis. Biotechnol Bioeng. 2011;108:655-65 pubmed publisher
    ..mobilis, thus leading to better understanding of this natural ethanol producer. In future, the current model could be employed to identify potential cell engineering targets, thereby enhancing the productivity of ethanol in Z. mobilis...
  64. Chew B, Lehming N. TFIIB/SUA7(E202G) is an allele-specific suppressor of TBP1(E186D). Biochem J. 2007;406:265-71 pubmed
    ..DNA microarray analysis indicated that the TBP1(E186D) temperature-sensitivity and slow-growth phenotypes might have been caused by insufficient amounts of Tbp1p for efficient transcription of the rRNA genes by RNA polymerase I. ..
  65. Guan X, Wenk M. Mass spectrometry-based profiling of phospholipids and sphingolipids in extracts from Saccharomyces cerevisiae. Yeast. 2006;23:465-77 pubmed
    ..The observed changes in lipid profiles are consistent with previous observations and extend our knowledge on in vivo substrate use under permissive growth conditions. ..
  66. Shen H, Mizushima N. At the end of the autophagic road: an emerging understanding of lysosomal functions in autophagy. Trends Biochem Sci. 2014;39:61-71 pubmed publisher
    ..We illustrate these findings in the context of the underlying molecular mechanisms and the relevance to human health and disease. ..
  67. Li X, Cai M. Recovery of the yeast cell cycle from heat shock-induced G(1) arrest involves a positive regulation of G(1) cyclin expression by the S phase cyclin Clb5. J Biol Chem. 1999;274:24220-31 pubmed
    ..Additional evidence is presented to support a role for Clb5 in maintaining the synchrony between budding and DNA synthesis during normal cell division as well. ..
  68. Zheng X, Wang Y, Wang Y. Hgc1, a novel hypha-specific G1 cyclin-related protein regulates Candida albicans hyphal morphogenesis. EMBO J. 2004;23:1845-56 pubmed
    ..C. albicans seems to have adapted a conserved strategy to control specifically hyphal morphogenesis. ..
  69. Huang B, Cai M. Pan1p: an actin director of endocytosis in yeast. Int J Biochem Cell Biol. 2007;39:1760-4 pubmed
    ..The phosphorylation of Pan1p is possibly reversed by the type 1 phosphatase Glc7p, which will allow Pan1p to be reused for coat assembly in the next round of endocytosis. ..
  70. Yu A, Pratomo Juwono N, Foo J, Leong S, Chang M. Metabolic engineering of Saccharomyces cerevisiae for the overproduction of short branched-chain fatty acids. Metab Eng. 2016;34:36-43 pubmed publisher
    ..We envision that this strategy could also be applied to the production of other chemicals in S. cerevisiae and may be extended to other microbes for strain improvement. ..
  71. Ang K, Ee G, Ang E, Koh E, Siew W, Chan Y, et al. Mediator acts upstream of the transcriptional activator Gal4. PLoS Biol. 2012;10:e1001290 pubmed publisher
    ..The ability of Mediator to control the protein degradation of transcriptional inhibitors indicates that Mediator is actually able to direct its own recruitment to gene promoters. ..
  72. Prasad R, Kawaguchi S, Ng D. Biosynthetic mode can determine the mechanism of protein quality control. Biochem Biophys Res Commun. 2012;425:689-95 pubmed publisher
    ..Targeting and integrating a misfolded protein to the ER membrane makes it an exclusive substrate of Doa10 whereas the soluble form of the same protein makes it a substrate of the San1/Ubr1 E3 system...
  73. Kanehara K, Xie W, Ng D. Modularity of the Hrd1 ERAD complex underlies its diverse client range. J Cell Biol. 2010;188:707-16 pubmed publisher
    ..The Hrd1 system does not directly evaluate the folding state of polypeptides. Instead, it does so indirectly, by recognizing specific embedded signals displayed upon misfolding. ..
  74. Rajmohan R, Meng L, Yu S, Thanabalu T. WASP suppresses the growth defect of Saccharomyces cerevisiae las17Delta strain in the presence of WIP. Biochem Biophys Res Commun. 2006;342:529-36 pubmed
  75. Li C, Wang Y, De Zheng X, Liang H, Tang J, Wang Y. The formin family protein CaBni1p has a role in cell polarity control during both yeast and hyphal growth in Candida albicans. J Cell Sci. 2005;118:2637-48 pubmed publisher
    ..Cabni1Delta exhibited reduced virulence in mice. These results indicate that the formins play an important role in Candida albicans polarized growth and CaBni1p's function is required for virulence...
  76. Huang B, Chua L, Bose N, Cai M. Negative regulation of the actin-regulating kinase Prk1p by patch localization-induced autophosphorylation. Traffic. 2009;10:35-41 pubmed publisher
    ..These results suggest that the autophosphorylation of Prk1p may represent a feedback mechanism possibly involved in fine-tuning the pace of progression during actin-coupled endocytosis. ..
  77. Zeng G, Huang B, Neo S, Wang J, Cai M. Scd5p mediates phosphoregulation of actin and endocytosis by the type 1 phosphatase Glc7p in yeast. Mol Biol Cell. 2007;18:4885-98 pubmed
    ..These results suggest that Glc7p antagonizes the activity of the Prk1p kinase in regulating the functions of Pan1p and possibly other actin- and endocytosis-related proteins. ..
  78. Zhang T, Lim H, Cheng C, Surana U. Deficiency of centromere-associated protein Slk19 causes premature nuclear migration and loss of centromeric elasticity. J Cell Sci. 2006;119:519-31 pubmed
    ..Our results suggest that Slk19 participates in regulating nuclear migration and, in conjunction with cohesin complex, may be involved in the maintenance of centromeric tensile strength to resist the pole-ward pull. ..
  79. Liu J, Wang H, Balasubramanian M. A checkpoint that monitors cytokinesis in Schizosaccharomyces pombe. J Cell Sci. 2000;113 ( Pt 7):1223-30 pubmed
    ..We conclude that an F-actin and Wee1p dependent checkpoint blocks G(2)/M transition until previous cytokinesis is completed. ..
  80. Tang B, Zhang T, Low D, Wong E, Horstmann H, Hong W. Mammalian homologues of yeast sec31p. An ubiquitously expressed form is localized to endoplasmic reticulum (ER) exit sites and is essential for ER-Golgi transport. J Biol Chem. 2000;275:13597-604 pubmed
    ..We conclude that Sec31A represents a functional mammalian homologue of yeast Sec31p. ..
  81. Naqvi S, Feng Q, Boulton V, Zahn R, Munn A. Vrp1p functions in both actomyosin ring-dependent and Hof1p-dependent pathways of cytokinesis. Traffic. 2001;2:189-201 pubmed
    ..Vrp1p thus acts in both actomyosin ring formation and function, as well as in Hof1p localisation during cytokinesis. ..
  82. Kamariah N, Eisenhaber F, Adhikari S, Eisenhaber B, Gruber G. Purification and crystallization of yeast glycosylphosphatidylinositol transamidase subunit PIG-S (PIG-S(71-467)). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67:896-9 pubmed publisher
    ..2 Å resolution with 91.6% completeness. The crystals belonged to space group C2, with unit-cell parameters a = 106.72, b = 59.33, c = 124.3 Å, β = 114.19°, and contained two molecules in the asymmetric unit. ..
  83. Rishikesan S, Thaker Y, Gruber G. NMR solution structure of subunit E (fragment E(1-69)) of the Saccharomyces cerevisiae V (1)V (O) ATPase. J Bioenerg Biomembr. 2011;43:187-93 pubmed publisher
    ..The molecule is amphipathic with hydrophobic residues at the N-terminus, predicted to interact with subunit C. The polar epitopes of E(1-69) are discussed as areas interacting with subunits G and H. ..
  84. Luhe A, Tan L, Wu J, Zhao H. Increase of ethanol tolerance of Saccharomyces cerevisiae by error-prone whole genome amplification. Biotechnol Lett. 2011;33:1007-11 pubmed publisher
    ..A few rounds of transformation led to the isolation of mutants that grew in 9% (v/v) ethanol and 100 g glucose l(-1) compared to untransformed yeast which grew only at 6% (v/v) ethanol and 100 g glucose l(-1). ..
  85. Chew B, Siew W, Xiao B, Lehming N. Transcriptional activation requires protection of the TATA-binding protein Tbp1 by the ubiquitin-specific protease Ubp3. Biochem J. 2010;431:391-9 pubmed publisher
    ..Chromatin immunoprecipitation showed that Ubp3 was recruited to the GAL1 and HIS3 promoters upon the induction of the respective gene, indicating that protection of promoter-bound Tbp1 by Ubp3 is required for transcriptional activation. ..