Experts and Doctors on cytoskeleton in Singapore, Central Singapore, Singapore


Locale: Singapore, Central Singapore, Singapore
Topic: cytoskeleton

Top Publications

  1. Leung T, Chen X, Manser E, Lim L. The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton. Mol Cell Biol. 1996;16:5313-27 pubmed
  2. Zhu H, Yeo S, Ng J, Htoon H, Beuerman R. Moesin as a key cytoskeleton regulator in corneal fibrosis. Ocul Surf. 2013;11:119-32 pubmed publisher
    ..58-, 0.53-, and 0.47-fold of control at the same PO days. : Moesin may be a potential drug target for inhibiting corneal fibrosis, and the details of moesin-related signaling pathways would be critical for understanding corneal fibrosis. ..
  3. Wang S, Low T, Nishimura Y, Golé L, Yu W, Motegi F. Cortical forces and CDC-42 control clustering of PAR proteins for Caenorhabditis elegans embryonic polarization. Nat Cell Biol. 2017;19:988-995 pubmed publisher
    ..The principles described here would apply to other pattern formation processes that rely on local modification of cortical actomyosin and PAR proteins. ..
  4. Tan S, Pan W, Ma G, Cai N, Leong K, Liao K. Viscoelastic behaviour of human mesenchymal stem cells. BMC Cell Biol. 2008;9:40 pubmed publisher
    ..Three other types of non-typical viscoelastic behavior of hMSCs were also seen. hMSCs behave as viscoelastic solid. Its viscoelstic behaviour are dependent on the structural integrity of the F-actin filaments and temperature. ..
  5. Huang B, Cai M. Pan1p: an actin director of endocytosis in yeast. Int J Biochem Cell Biol. 2007;39:1760-4 pubmed
    ..The phosphorylation of Pan1p is possibly reversed by the type 1 phosphatase Glc7p, which will allow Pan1p to be reused for coat assembly in the next round of endocytosis. ..
  6. Beguin P, Mahalakshmi R, Nagashima K, Cher D, Takahashi A, Yamada Y, et al. 14-3-3 and calmodulin control subcellular distribution of Kir/Gem and its regulation of cell shape and calcium channel activity. J Cell Sci. 2005;118:1923-34 pubmed
  7. Rajmohan R, Wong M, Meng L, Munn A, Thanabalu T. Las17p-Vrp1p but not Las17p-Arp2/3 interaction is important for actin patch polarization in yeast. Biochim Biophys Acta. 2009;1793:825-35 pubmed publisher
    ..Using these mutant and truncated forms of yeast WASP we provide novel evidence that WASP interaction with WIP is more important than interaction with Arp2/3 for polarized actin assembly and endocytosis in yeast. ..
  8. Tan I, Lai J, Yong J, Li S, Leung T. Chelerythrine perturbs lamellar actomyosin filaments by selective inhibition of myotonic dystrophy kinase-related Cdc42-binding kinase. FEBS Lett. 2011;585:1260-8 pubmed publisher
    ..Its ability to block cellular activity of MRCK resulted in the specific loss of NM II-associated MLC phosphorylation in the lamella, and the consequential suppression of cell migration. ..
  9. Wang T, Hong W. Interorganellar regulation of lysosome positioning by the Golgi apparatus through Rab34 interaction with Rab-interacting lysosomal protein. Mol Biol Cell. 2002;13:4317-32 pubmed
    ..This biological activity, mediated by Rab34-RILP interaction, is dependent on Lys82 in the switch I region. Our results have uncovered a novel mechanism for the Golgi apparatus to regulate the spatial distribution of another organelle. ..

More Information


  1. Tang H, Xu J, Cai M. Pan1p, End3p, and S1a1p, three yeast proteins required for normal cortical actin cytoskeleton organization, associate with each other and play essential roles in cell wall morphogenesis. Mol Cell Biol. 2000;20:12-25 pubmed
    ..These observations suggest that the Pan1p-End3p-Sla1p complex is required not only for normal actin cytoskeleton organization but also for normal cell wall morphogenesis in yeast. ..
  2. Pontes B, Monzo P, Golé L, Le Roux A, Kosmalska A, Tam Z, et al. Membrane tension controls adhesion positioning at the leading edge of cells. J Cell Biol. 2017;216:2959-2977 pubmed publisher
    ..We propose that the mechanical signal of membrane tension exerts upstream control in mechanotransduction by periodically compressing and relaxing the lamellipodium, leading to the positioning of adhesions at the leading edge of cells. ..
  3. Zhang S, Guo T, Chan H, Sze S, Koh C. Integrative transcriptome and proteome study to identify the signaling network regulated by POPX2 phosphatase. J Proteome Res. 2013;12:2525-36 pubmed publisher
    ..Taken together, our data suggest that POPX2 might regulate cell motility through its regulation of the MAPK1/3, leading to changes in the cytoskeleton and cell motility. ..
  4. Zheng W, Wang Z, Collins J, Andrews R, STEMPLE D, Gong Z. Comparative transcriptome analyses indicate molecular homology of zebrafish swimbladder and mammalian lung. PLoS ONE. 2011;6:e24019 pubmed publisher
    ..Our comparative analyses also provide molecular evidence of the relatedness of the fish swimbladder and mammalian lung...
  5. Feng Z, Chen B, Tang S, Liao K, Chen W, Chan V. Effect of cytoskeleton inhibitors on deadhesion kinetics of HepG2 cells on biomimetic surface. Colloids Surf B Biointerfaces. 2010;75:67-74 pubmed publisher
    ..Overall, our biophysical approaches have been proven as a highly quantitative platform for elucidating the interfacial properties of adherent cells on biomimetic surfaces under cytoskeleton disruption. ..
  6. Chu J, Ng M. Infectious entry of West Nile virus occurs through a clathrin-mediated endocytic pathway. J Virol. 2004;78:10543-55 pubmed publisher
    ..In situ hybridization of DNA probes specific for viral RNA demonstrated the trafficking of uncoated viral RNA genomes to the endoplasmic reticulum...
  7. Kausalya P, Phua D, Hunziker W. Association of ARVCF with zonula occludens (ZO)-1 and ZO-2: binding to PDZ-domain proteins and cell-cell adhesion regulate plasma membrane and nuclear localization of ARVCF. Mol Biol Cell. 2004;15:5503-15 pubmed
    ..Interactions with ZO-1 and ZO-2, in particular, may mediate recruitment of ARVCF to the plasma membrane and the nucleus, respectively, possibly in response to cell-cell adhesion cues. ..
  8. Fu D, Thomson C, Lunny D, Dopping Hepenstal P, McGrath J, Smith F, et al. Keratin 9 is required for the structural integrity and terminal differentiation of the palmoplantar epidermis. J Invest Dermatol. 2014;134:754-763 pubmed publisher
    ..Together, our data demonstrate that complete ablation of K9 is not tolerable in vivo and that K9 is required for terminal differentiation and maintaining the mechanical integrity of palmoplantar epidermis. ..
  9. Harden N, Ricos M, Ong Y, Chia W, Lim L. Participation of small GTPases in dorsal closure of the Drosophila embryo: distinct roles for Rho subfamily proteins in epithelial morphogenesis. J Cell Sci. 1999;112 ( Pt 3):273-84 pubmed
    ..Rather, our results suggest that while all &Rgr; subfamily p21s tested are required for dorsal closure, they act largely in parallel. ..
  10. Zhou Y, Guy G, Low B. BNIP-Salpha induces cell rounding and apoptosis by displacing p50RhoGAP and facilitating RhoA activation via its unique motifs in the BNIP-2 and Cdc42GAP homology domain. Oncogene. 2006;25:2393-408 pubmed
    ..This concerted mechanism could allow effective propagation of the RhoA pathway for cell rounding and apoptosis. ..
  11. Srinivasan R, Mishra M, Murata Hori M, Balasubramanian M. Filament formation of the Escherichia coli actin-related protein, MreB, in fission yeast. Curr Biol. 2007;17:266-72 pubmed
    ..The cell ends appeared to provide a physical barrier for bundle elongation. These experiments provide new insights into the mechanism of assembly and organization of the bacterial actin cytoskeleton. ..
  12. Horn H, Kim D, Wright G, Wong E, Stewart C, Burke B, et al. A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton. J Cell Biol. 2013;202:1023-39 pubmed publisher
    ..These findings demonstrate that telomere attachment to the NE is insufficient to promote pairing and that telomere attachment sites must be coupled to cytoplasmic dynein and the microtubule system to ensure meiotic progression...
  13. Yow S, Quek C, Yim E, Lim C, Leong K. Collagen-based fibrous scaffold for spatial organization of encapsulated and seeded human mesenchymal stem cells. Biomaterials. 2009;30:1133-42 pubmed publisher
    ..It lays the foundation for building fibrous scaffold that permits 3D spatial cellular organization and multi-cellular tissue development. ..
  14. Yang W, Guo X, Thein S, Xu F, Sugii S, Baas P, et al. Regulation of adipogenesis by cytoskeleton remodelling is facilitated by acetyltransferase MEC-17-dependent acetylation of ?-tubulin. Biochem J. 2013;449:605-12 pubmed publisher
  15. Balasubramanian M, Srinivasan R, Huang Y, Ng K. Comparing contractile apparatus-driven cytokinesis mechanisms across kingdoms. Cytoskeleton (Hoboken). 2012;69:942-56 pubmed publisher
    ..Intriguingly, there are life forms on this planet that appear to lack molecules currently known to participate in cytokinesis and how these cells perform cytokinesis remains a mystery waiting to be unravelled...
  16. Srinivasan R, Mishra M, Wu L, Yin Z, Balasubramanian M. The bacterial cell division protein FtsZ assembles into cytoplasmic rings in fission yeast. Genes Dev. 2008;22:1741-6 pubmed publisher
    ..We conclude that FtsZ rings can assemble in the absence of all other bacterial cytokinetic proteins and that the process might involve hydrolysis of FtsZ-bound GTP and lateral associations between FtsZ filaments. ..
  17. Vjestica A, Tang X, Oliferenko S. The actomyosin ring recruits early secretory compartments to the division site in fission yeast. Mol Biol Cell. 2008;19:1125-38 pubmed publisher
    ..Cdc15p overexpression was sufficient to induce tER polarization in interphase. Thus, fission yeast polarizes its entire secretory machinery to the cell division site by utilizing molecular cues provided by the actomyosin ring. ..
  18. Mishra M, D souza V, Chang K, Huang Y, Balasubramanian M. Hsp90 protein in fission yeast Swo1p and UCS protein Rng3p facilitate myosin II assembly and function. Eukaryot Cell. 2005;4:567-76 pubmed
    ..Our analyses of Myo2p, Swo1p, and the UCS domain protein Rng3p establish that Swo1p and Rng3p collaborate in vivo to modulate myosin II function. ..
  19. Tang H, Munn A, Cai M. EH domain proteins Pan1p and End3p are components of a complex that plays a dual role in organization of the cortical actin cytoskeleton and endocytosis in Saccharomyces cerevisiae. Mol Cell Biol. 1997;17:4294-304 pubmed
  20. Qiu W, Neo S, Yu X, Cai M. A novel septin-associated protein, Syp1p, is required for normal cell cycle-dependent septin cytoskeleton dynamics in yeast. Genetics. 2008;180:1445-57 pubmed publisher
    ..These results suggest that Syp1p is involved in the regulation of cell cycle-dependent dynamics of the septin cytoskeleton in yeast. ..
  21. Radhakrishnan A, Jokhun D, Venkatachalapathy S, Shivashankar G. Nuclear Positioning and Its Translational Dynamics Are Regulated by Cell Geometry. Biophys J. 2017;112:1920-1928 pubmed publisher
    ..These biophysical signatures have the potential to be used as an ultrasensitive single-cell assay for early disease diagnostics. ..
  22. Mirza H, Wu Z, Teo J, Tan K. Statin pleiotropy prevents rho kinase-mediated intestinal epithelial barrier compromise induced by Blastocystis cysteine proteases. Cell Microbiol. 2012;14:1474-84 pubmed publisher
    ..Simvastatin prevented parasite-induced barrier-compromise, suggesting a therapeutic potential of statins in intestinal infections. ..
  23. Patkar R, Suresh A, Naqvi N. MoTea4-mediated polarized growth is essential for proper asexual development and pathogenesis in Magnaporthe oryzae. Eukaryot Cell. 2010;9:1029-38 pubmed publisher
    ..We show that MoTea4 plays an important role in organizing the F-actin cytoskeleton and is essentially required for polarized growth and morphogenesis during asexual and pathogenic development in Magnaporthe...
  24. Huang B, Zeng G, Ng A, Cai M. Identification of novel recognition motifs and regulatory targets for the yeast actin-regulating kinase Prk1p. Mol Biol Cell. 2003;14:4871-84 pubmed
    ..These results allowed us to conclude that Scd5p is likely another regulatory target of Prk1p. ..
  25. Zeng L, Si X, Yu W, Le H, Ng K, Teng R, et al. PTP alpha regulates integrin-stimulated FAK autophosphorylation and cytoskeletal rearrangement in cell spreading and migration. J Cell Biol. 2003;160:137-46 pubmed
    ..Our paper establishes that PTP alpha is required for early integrin-proximal events, acting upstream of FAK to affect the timely and efficient phosphorylation of FAK Tyr-397. ..
  26. Veronika M, Welsch R, Ng A, Matsudaira P, Rajapakse J. Correlation of cell membrane dynamics and cell motility. BMC Bioinformatics. 2011;12 Suppl 13:S19 pubmed publisher
    ..Our findings are consistent with published literature and we also identified that motility patterns are influenced by edge features from initial time points compared to later sampling intervals. ..
  27. Zeng G, Yu X, Cai M. Regulation of yeast actin cytoskeleton-regulatory complex Pan1p/Sla1p/End3p by serine/threonine kinase Prk1p. Mol Biol Cell. 2001;12:3759-72 pubmed
    ..These results lend a strong support to the model that the phosphorylation of the Pan1p/Sla1p/End3p complex by Prk1p is one of the important mechanisms by which the organization and functions of the actin cytoskeleton are regulated. ..
  28. Huang M, Jongejan L, Zheng H, Zhang L, Bol J. Intracellular localization and movement phenotypes of alfalfa mosaic virus movement protein mutants. Mol Plant Microbe Interact. 2001;14:1063-74 pubmed
    ..Evidence was obtained that tubular structures on the surface of transfected protoplasts contain ER- or plasmalemma-derived material...
  29. Biswas K, Zaidel Bar R. Early events in the assembly of E-cadherin adhesions. Exp Cell Res. 2017;358:14-19 pubmed publisher
    ..Here, we describe an updated view of the process of E-cadherin adhesion formation with an emphasis on the role of molecular mobility, clustering, and active cellular processes. ..
  30. Kirilly D, Gu Y, Huang Y, Wu Z, Bashirullah A, Low B, et al. A genetic pathway composed of Sox14 and Mical governs severing of dendrites during pruning. Nat Neurosci. 2009;12:1497-505 pubmed publisher
    ..Thus, our findings indicate that a previously unknown pathway composed of Sox14 and its cytoskeletal target Mical governs dendrite severing. ..
  31. Zhao Z, Manser E, Loo T, Lim L. Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal complex disassembly. Mol Cell Biol. 2000;20:6354-63 pubmed
    ..We propose that GIT1 and FAK cooperate to promote motility both by directly regulating focal complex dynamics and by the activation of Rac. ..
  32. Ng K, Srinivas V, Srinivasan R, Balasubramanian M. The Nitrosopumilus maritimus CdvB, but not FtsZ, assembles into polymers. Archaea. 2013;2013:104147 pubmed publisher
    ..maritimus. Thus, we propose that it is Cdv proteins, rather than FtsZ, that function as the cell division apparatus in N. maritimus. ..
  33. Leung T, Chen X, Tan I, Manser E, Lim L. Myotonic dystrophy kinase-related Cdc42-binding kinase acts as a Cdc42 effector in promoting cytoskeletal reorganization. Mol Cell Biol. 1998;18:130-40 pubmed
    ..These results suggest that MRCK alpha may act as a downstream effector of Cdc42 in cytoskeletal reorganization. ..
  34. Gore A, Sampath K. Localization of transcripts of the zebrafish morphogen Squint is dependent on egg activation and the microtubule cytoskeleton. Mech Dev. 2002;112:153-6 pubmed
    ..These results indicate that sqt transcripts are translocated by an RNA localization pathway which is initiated upon egg activation, and that sqt RNA localization through this pathway is mediated via the microtubule cytoskeleton. ..
  35. Ricos M, Harden N, Sem K, Lim L, Chia W. Dcdc42 acts in TGF-beta signaling during Drosophila morphogenesis: distinct roles for the Drac1/JNK and Dcdc42/TGF-beta cascades in cytoskeletal regulation. J Cell Sci. 1999;112 ( Pt 8):1225-35 pubmed
    ..Signaling in the transforming-growth-factor-beta-like pathway is mediated by Dcdc42, and acts during the closure process to control the mechanics of the migration process, most likely via its putative effector kinase DPAK. ..
  36. Zeng G, Cai M. Regulation of the actin cytoskeleton organization in yeast by a novel serine/threonine kinase Prk1p. J Cell Biol. 1999;144:71-82 pubmed
    ..Based on these findings, we propose that the Prk1 protein kinase regulates the actin cytoskeleton organization by modulating the activities of some actin cytoskeleton-related proteins such as Pan1p/End3p. ..
  37. Yu X, Cai M. The yeast dynamin-related GTPase Vps1p functions in the organization of the actin cytoskeleton via interaction with Sla1p. J Cell Sci. 2004;117:3839-53 pubmed
    ..These findings suggest that the functions of the dynamin-related protein Vps1p in actin cytoskeleton dynamics and vacuolar protein sorting are probably related to each other. ..
  38. Moh M, Tian Q, Zhang T, Lee L, Shen S. The immunoglobulin-like cell adhesion molecule hepaCAM modulates cell adhesion and motility through direct interaction with the actin cytoskeleton. J Cell Physiol. 2009;219:382-91 pubmed publisher
    ..The data suggest that an intact hepaCAM protein is critical for establishing a stable physical association with the actin cytoskeleton; and such association is important for modulating hepaCAM-mediated cell adhesion and motility. ..