Experts and Doctors on cytokinesis in Singapore, Central Singapore, Singapore


Locale: Singapore, Central Singapore, Singapore
Topic: cytokinesis

Top Publications

  1. Wachtler V, Huang Y, Karagiannis J, Balasubramanian M. Cell cycle-dependent roles for the FCH-domain protein Cdc15p in formation of the actomyosin ring in Schizosaccharomyces pombe. Mol Biol Cell. 2006;17:3254-66 pubmed
    ..This suggests a mechanism where both SIN and Clp1p/Flp1p contribute to maintenance of the actomyosin ring in late mitosis through Cdc15p, possibly by regulating its phosphorylation status. ..
  2. Chew T, Balasubramanian M. Nuc2p, a subunit of the anaphase-promoting complex, inhibits septation initiation network following cytokinesis in fission yeast. PLoS Genet. 2008;4:e17 pubmed publisher
    ..We propose that Nuc2p, by inhibiting the SIN after cell division, prevents further deleterious cytokinetic events, thereby contributing to genome stability. ..
  3. Chin C, Bennett A, Ma W, Hall M, Yeong F. Dependence of Chs2 ER export on dephosphorylation by cytoplasmic Cdc14 ensures that septum formation follows mitosis. Mol Biol Cell. 2012;23:45-58 pubmed publisher
    ..Thus, by requiring declining Cdk1 activity and Cdc14 nuclear release for Chs2 ER export, cells ensure that septum formation is contingent upon chromosome separation and exit from mitosis. ..
  4. Mishra M, Karagiannis J, Trautmann S, Wang H, McCollum D, Balasubramanian M. The Clp1p/Flp1p phosphatase ensures completion of cytokinesis in response to minor perturbation of the cell division machinery in Schizosaccharomyces pombe. J Cell Sci. 2004;117:3897-910 pubmed
    ..We conclude that the Clp1p/Flp1p-dependent cytokinesis checkpoint provides a previously unrecognized cell survival advantage when the cell division apparatus is mildly perturbed. ..
  5. Bimbo A, Liu J, Balasubramanian M. Roles of Pdk1p, a fission yeast protein related to phosphoinositide-dependent protein kinase, in the regulation of mitosis and cytokinesis. Mol Biol Cell. 2005;16:3162-75 pubmed
    ..We also present evidence that the effects of Pdk1p on cytokinesis are likely mediated via the fission yeast anillin-related protein, Mid1p, and the septation initiation network. ..
  6. Mishra M, Kashiwazaki J, Takagi T, Srinivasan R, Huang Y, Balasubramanian M, et al. In vitro contraction of cytokinetic ring depends on myosin II but not on actin dynamics. Nat Cell Biol. 2013;15:853-9 pubmed publisher
    ..Our work provides the beginnings of the definition of a minimal contraction-competent cytokinetic ring apparatus...
  7. Mishra M, Karagiannis J, Sevugan M, Singh P, Balasubramanian M. The 14-3-3 protein rad24p modulates function of the cdc14p family phosphatase clp1p/flp1p in fission yeast. Curr Biol. 2005;15:1376-83 pubmed
    ..Furthermore, SIN-dependent nuclear exclusion of Clp1p is dependent on Rad24p function. We conclude that Rad24p-mediated cytoplasmic retention of Clp1p/Flp1p is important for cell viability upon stress to the division apparatus. ..
  8. Huang Y, Chew T, Ge W, Balasubramanian M. Polarity determinants Tea1p, Tea4p, and Pom1p inhibit division-septum assembly at cell ends in fission yeast. Dev Cell. 2007;12:987-96 pubmed
    ..Since dual regulation of division-site placement is also seen in nematodes, such regulation might be a general feature of eukaryotic cytokinesis. ..
  9. Ge W, Balasubramanian M. Pxl1p, a paxillin-related protein, stabilizes the actomyosin ring during cytokinesis in fission yeast. Mol Biol Cell. 2008;19:1680-92 pubmed publisher
    ..Collectively, these results suggest that Pxl1p might cooperate with type II myosin and Rng2p-IQGAP to regulate actomyosin ring constriction as well as to maintain its integrity during constriction. ..

More Information


  1. Singh N, Shao N, McLean J, Sevugan M, Ren L, Chew T, et al. SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast. Curr Biol. 2011;21:1968-78 pubmed publisher
    ..Because Cdc7p does not bind to GDP-Spg1p, we propose that the SIP-mediated Cdc11p dephosphorylation and the resulting recruitment of Byr4p are among the earliest steps in the establishment of SIN asymmetry. ..
  2. Oliferenko S, Chew T, Balasubramanian M. Positioning cytokinesis. Genes Dev. 2009;23:660-74 pubmed publisher
    ..We also describe cytokinesis-positioning mechanisms that are not adequately explained by studies in model organisms and model cell types. ..
  3. Zhang D, Vjestica A, Oliferenko S. The cortical ER network limits the permissive zone for actomyosin ring assembly. Curr Biol. 2010;20:1029-34 pubmed publisher
    ..We propose that the cortical ER network restricts the lateral motion of Mid1p and hence generates a permissive zone for actomyosin ring assembly precisely at the cell equator. ..
  4. Zhu X, Wang J, Moriguchi K, Liow L, Ahmed S, Kaverina I, et al. Proper regulation of Cdc42 activity is required for tight actin concentration at the equator during cytokinesis in adherent mammalian cells. Exp Cell Res. 2011;317:2384-9 pubmed publisher
    ..Notably, our observations also suggest that tight actin concentration is not essential for cytokinesis in adherent mammalian cells. ..
  5. Zhang D, Bidone T, Vavylonis D. ER-PM Contacts Define Actomyosin Kinetics for Proper Contractile Ring Assembly. Curr Biol. 2016;26:647-53 pubmed publisher
    ..We propose that ER-PM contacts have evolved as important physical modulators to ensure robust ring assembly. ..
  6. Subramanian D, Huang J, Sevugan M, Robinson R, Balasubramanian M, Tang X. Insight into actin organization and function in cytokinesis from analysis of fission yeast mutants. Genetics. 2013;194:435-46 pubmed publisher
    ..This mutant bank should be a valuable resource to study the physiological and biochemical functions of actin...
  7. Huang J, Huang Y, Yu H, Subramanian D, Padmanabhan A, Thadani R, et al. Nonmedially assembled F-actin cables incorporate into the actomyosin ring in fission yeast. J Cell Biol. 2012;199:831-47 pubmed publisher
    ..Our results, together with findings in animal cells, suggest that de novo F-actin assembly at the division site and directed transport of F-actin cables assembled elsewhere can contribute to ring assembly. ..
  8. Yan H, Ge W, Chew T, Chow J, McCollum D, Neiman A, et al. The meiosis-specific Sid2p-related protein Slk1p regulates forespore membrane assembly in fission yeast. Mol Biol Cell. 2008;19:3676-90 pubmed publisher
    ..These studies thereby provide a novel link between the SIN and vesicle trafficking during cytokinesis. ..
  9. Vjestica A, Tang X, Oliferenko S. The actomyosin ring recruits early secretory compartments to the division site in fission yeast. Mol Biol Cell. 2008;19:1125-38 pubmed publisher
    ..Cdc15p overexpression was sufficient to induce tER polarization in interphase. Thus, fission yeast polarizes its entire secretory machinery to the cell division site by utilizing molecular cues provided by the actomyosin ring. ..
  10. Karagiannis J, Bimbo A, Rajagopalan S, Liu J, Balasubramanian M. The nuclear kinase Lsk1p positively regulates the septation initiation network and promotes the successful completion of cytokinesis in response to perturbation of the actomyosin ring in Schizosaccharomyces pombe. Mol Biol Cell. 2005;16:358-71 pubmed
    ..Our studies also establish that actomyosin ring maintenance and nuclear cycle delay in response to cytokinetic perturbation can be genetically resolved into independent pathways. ..
  11. Loo T, Balasubramanian M. Schizosaccharomyces pombe Pak-related protein, Pak1p/Orb2p, phosphorylates myosin regulatory light chain to inhibit cytokinesis. J Cell Biol. 2008;183:785-93 pubmed publisher
    ..This study reveals a new mechanism involving Pak1p kinase that helps ensure the fidelity of cytokinesis. ..
  12. Calvert M, Wright G, Leong F, Chiam K, Chen Y, Jedd G, et al. Myosin concentration underlies cell size-dependent scalability of actomyosin ring constriction. J Cell Biol. 2011;195:799-813 pubmed publisher
    ..Because the mechanical characteristics of contractile rings are conserved, we predict that these findings will be relevant to actomyosin ring constriction in other cell types. ..
  13. Balasubramanian M, Srinivasan R, Huang Y, Ng K. Comparing contractile apparatus-driven cytokinesis mechanisms across kingdoms. Cytoskeleton (Hoboken). 2012;69:942-56 pubmed publisher
    ..Intriguingly, there are life forms on this planet that appear to lack molecules currently known to participate in cytokinesis and how these cells perform cytokinesis remains a mystery waiting to be unravelled...
  14. Ge W, Chew T, Wachtler V, Naqvi S, Balasubramanian M. The novel fission yeast protein Pal1p interacts with Hip1-related Sla2p/End4p and is involved in cellular morphogenesis. Mol Biol Cell. 2005;16:4124-38 pubmed
    ..Thus, overlapping mechanisms involving Pal1p, Tea1p, and Sla2p contribute to the establishment of cylindrical cellular morphology, which is important for proper spatial regulation of cytokinesis. ..
  15. McNamara C, Lee M, Lim C, Lim S, Roland J, Simon O, et al. Targeting Plasmodium PI(4)K to eliminate malaria. Nature. 2013;504:248-253 pubmed publisher
    ..Collectively, our data define PI(4)K as a key Plasmodium vulnerability, opening up new avenues of target-based discovery to identify drugs with an ideal activity profile for the prevention, treatment and elimination of malaria. ..