Experts and Doctors on transgenes in Moscow, Russia
Locale: Moscow, Russia
Logunov D, Ilyinskaya G, Cherenova L, Verhovskaya L, Shmarov M, Chumakov P, et al
. Restoration of p53 tumor-suppressor activity in human tumor cells in vitro and in their xenografts in vivo by recombinant avian adenovirus CELO-p53. Gene Ther. 2004;11:79-84 pubmed
Rodin S, Kyrchanova O, Pomerantseva E, Parshikov A, Georgiev P. New properties of Drosophila fab-7 insulator. Genetics. 2007;177:113-21 pubmed
..It seems likely that Fab-7 strengthens the relatively weak white promoter, which leads to the efficient enhancer-promoter interaction and insulator bypass...
Berdichevets I, Shimshilashvili H, Gerasymenko I, Sindarovska Y, Sheludko Y, Goldenkova Pavlova I. Multiplex PCR assay for detection of recombinant genes encoding fatty acid desaturases fused with lichenase reporter protein in GM plants. Anal Bioanal Chem. 2010;397:2289-93 pubmed publisher
..Here, we report a multiplex polymerase chain reaction method for detection of desA and desC desaturase genes of cyanobacteria Synechocystis sp. PCC6803 and Synechococcus vulcanus, respectively, fused to licBM3 reporter in GM plants. ..
Olovnikov I, Ryazansky S, Shpiz S, Lavrov S, Abramov Y, Vaury C, et al
. De novo piRNA cluster formation in the Drosophila germ line triggered by transgenes containing a transcribed transposon fragment. Nucleic Acids Res. 2013;41:5757-68 pubmed publisher
..We show that transgene-derived hsp70 piRNAs stimulate in trans cleavage of cognate endogenous transcripts with subsequent processing of the non-homologous parts of these transcripts into piRNAs. ..
Golovnin A, Biryukova I, Birukova I, Romanova O, Silicheva M, Parshikov A, et al
. An endogenous Su(Hw) insulator separates the yellow gene from the Achaete-scute gene complex in Drosophila. Development. 2003;130:3249-58 pubmed
..Genetic analysis shows that at least two proteins, Suppressor of Hairy wing and Modifier of MDG4, required for the activity of this insulator, are involved in the transcriptional regulation of Achaete-scute. ..
Kyrchanova O, Toshchakov S, Parshikov A, Georgiev P. Study of the functional interaction between Mcp insulators from the Drosophila bithorax complex: effects of insulator pairing on enhancer-promoter communication. Mol Cell Biol. 2007;27:3035-43 pubmed
..These results indicate that the relative placement and orientation of insulator-like elements can determine proper enhancer-promoter communication. ..
Melnikova L, Kostuchenko M, Silicheva M, Georgiev P. Drosophila gypsy insulator and yellow enhancers regulate activity of yellow promoter through the same regulatory element. Chromosoma. 2008;117:137-45 pubmed
..These finding suggest that proteins bound to the -100 to -69 sequence are essential for communication between the yellow promoter and upstream regulatory elements. ..
Golovnin A, Melnikova L, Volkov I, Kostuchenko M, Galkin A, Georgiev P. 'Insulator bodies' are aggregates of proteins but not of insulators. EMBO Rep. 2008;9:440-5 pubmed publisher
..Hence, it is not the functional genomic insulators but rather aggregated proteins that make the so-called 'insulator bodies'. ..
Kostyuchenko M, Savitskaya E, Koryagina E, Melnikova L, Karakozova M, Georgiev P. Zeste can facilitate long-range enhancer-promoter communication and insulator bypass in Drosophila melanogaster. Chromosoma. 2009;118:665-74 pubmed publisher
..Zeste is also required for insulator bypass by the eye enhancer. Taken together, these results show that Zeste can support specific long-range interactions between enhancers and promoters. ..