Experts and Doctors on zebrafish in Leiden, South Holland, Netherlands


Locale: Leiden, South Holland, Netherlands
Topic: zebrafish

Top Publications

  1. Meijer A. Protection and pathology in TB: learning from the zebrafish model. Semin Immunopathol. 2016;38:261-73 pubmed publisher
    ..As a result, zebrafish models are now increasingly used to explore strategies for adjunctive therapy of tuberculosis with host-directed drugs. ..
  2. Carvalho R, Kleijn J, Meijer A, Verbeek F. Modeling innate immune response to early Mycobacterium infection. Comput Math Methods Med. 2012;2012:790482 pubmed publisher
    ..Thereby it supports the unraveling of the mechanisms of tuberculosis infection. ..
  3. Schaaf M, Champagne D, van Laanen I, van Wijk D, Meijer A, Meijer O, et al. Discovery of a functional glucocorticoid receptor beta-isoform in zebrafish. Endocrinology. 2008;149:1591-9 pubmed
    ..Thus, for the first time, a GR beta-isoform has been identified in a nonhuman animal species, shedding new light on the relevance of this GR splice variant and providing a versatile animal model for studies on the GR system. ..
  4. Stockhammer O, Zakrzewska A, Hegedus Z, Spaink H, Meijer A. Transcriptome profiling and functional analyses of the zebrafish embryonic innate immune response to Salmonella infection. J Immunol. 2009;182:5641-53 pubmed publisher
    ..Finally, we showed that induction of il1b, mmp9, and irak3 requires Myd88-dependent signaling, while ifn1 and il8 were induced Myd88 independently during Salmonella infection. ..
  5. Champagne D, Hoefnagels C, de Kloet R, Richardson M. Translating rodent behavioral repertoire to zebrafish (Danio rerio): relevance for stress research. Behav Brain Res. 2010;214:332-42 pubmed publisher
    ..These findings are consistent with previous findings in rodents and support the usefulness of such behavioral paradigms in zebrafish. ..
  6. Mohseny A, Xiao W, Carvalho R, Spaink H, Hogendoorn P, Cleton Jansen A. An osteosarcoma zebrafish model implicates Mmp-19 and Ets-1 as well as reduced host immune response in angiogenesis and migration. J Pathol. 2012;227:245-53 pubmed publisher
    ..These findings contribute to the identification of genetic events involved in angiogenesis, migration, and host response providing targets as well as an appropriate model for high-throughput drug screens. ..
  7. Cui C, Benard E, Kanwal Z, Stockhammer O, van der Vaart M, Zakrzewska A, et al. Infectious disease modeling and innate immune function in zebrafish embryos. Methods Cell Biol. 2011;105:273-308 pubmed publisher
    ..Here we provide practical guidelines for the application of these methodologies and review the current state of the art in zebrafish infectious disease research. ..
  8. Marques I, Leito J, Spaink H, Testerink J, Jaspers R, Witte F, et al. Transcriptome analysis of the response to chronic constant hypoxia in zebrafish hearts. J Comp Physiol B. 2008;178:77-92 pubmed
    ..We have identified here many novel genes involved in the response to CCH in the heart, which may have potential clinical implications in the future. ..
  9. Woltering J, Durston A. MiR-10 represses HoxB1a and HoxB3a in zebrafish. PLoS ONE. 2008;3:e1396 pubmed publisher

More Information


  1. Rauwerda H, Pagano J, de Leeuw W, Ensink W, Nehrdich U, de Jong M, et al. Transcriptome dynamics in early zebrafish embryogenesis determined by high-resolution time course analysis of 180 successive, individual zebrafish embryos. BMC Genomics. 2017;18:287 pubmed publisher
  2. Masud S, Torraca V, Meijer A. Modeling Infectious Diseases in the Context of a Developing Immune System. Curr Top Dev Biol. 2017;124:277-329 pubmed publisher
    ..These studies are providing a mechanistic basis for developing novel therapeutic approaches to control infectious diseases. ..
  3. Basters A, Geurink P, Röcker A, Witting K, Tadayon R, Hess S, et al. Structural basis of the specificity of USP18 toward ISG15. Nat Struct Mol Biol. 2017;24:270-278 pubmed publisher
    ..Our results may provide a structural basis for the development of new drugs modulating ISG15 linkage. ..
  4. Naber H, Drabsch Y, Snaar Jagalska B, Ten Dijke P, van Laar T. Snail and Slug, key regulators of TGF-?-induced EMT, are sufficient for the induction of single-cell invasion. Biochem Biophys Res Commun. 2013;435:58-63 pubmed publisher
    ..Slug and Snail are sufficient for the induction of single-cell invasion in an in vitro invasion assay and in an embryonic zebrafish xenograft model. ..
  5. Ali S, Champagne D, Alia A, Richardson M. Large-scale analysis of acute ethanol exposure in zebrafish development: a critical time window and resilience. PLoS ONE. 2011;6:e20037 pubmed publisher
    ..We also identify a critical time window (prim-6 and prim-16) for ethanol sensitivity. Finally, our identification of a wide phenotypic spectrum is reminiscent of human FAS, and may provide a useful model for studying disease resilience. ..
  6. Wiweger M, Zhao Z, van Merkesteyn R, Roehl H, Hogendoorn P. HSPG-deficient zebrafish uncovers dental aspect of multiple osteochondromas. PLoS ONE. 2012;7:e29734 pubmed publisher
    ..The presence of the malformed and/or displaced teeth with abnormal enamel was declared by half of the respondents indicating that MO might indeed be also associated with dental problems. ..
  7. Carvalho R, de Sonneville J, Stockhammer O, Savage N, Veneman W, Ottenhoff T, et al. A high-throughput screen for tuberculosis progression. PLoS ONE. 2011;6:e16779 pubmed publisher
    ..Importantly, we use reference compounds to validate our system in the testing of molecules that prevent tuberculosis progression, making it highly suited for investigating novel anti-tuberculosis compounds in vivo. ..
  8. Ali S, van Mil H, Richardson M. Large-scale assessment of the zebrafish embryo as a possible predictive model in toxicity testing. PLoS ONE. 2011;6:e21076 pubmed publisher
    ..However, the correlation between zebrafish and rodent toxicity varies considerably between individual compounds and compound class. We discuss the strengths and limitations of the zebrafish model in light of these findings. ..
  9. Sharif F, Porta F, Meijer A, Kros A, Richardson M. Mesoporous silica nanoparticles as a compound delivery system in zebrafish embryos. Int J Nanomedicine. 2012;7:1875-90 pubmed publisher
    ..Our results show that MSNPs can be used to deliver bioactive compounds into zebrafish larvae without producing higher mortality or gross evidence of teratogenicity...
  10. Orlova V, Drabsch Y, Freund C, Petrus Reurer S, van den Hil F, Muenthaisong S, et al. Functionality of endothelial cells and pericytes from human pluripotent stem cells demonstrated in cultured vascular plexus and zebrafish xenografts. Arterioscler Thromb Vasc Biol. 2014;34:177-86 pubmed publisher
  11. Meijer A, Spaink H. Host-pathogen interactions made transparent with the zebrafish model. Curr Drug Targets. 2011;12:1000-17 pubmed
    ..We summarize recent insights gained from the use of bacterial infection models, particularly the Mycobacterium marinum model that illustrate the potential of the zebrafish model for high-throughput antimicrobial drug screening. ..
  12. Tulotta C, Stefanescu C, Beletkaia E, Bussmann J, Tarbashevich K, Schmidt T, et al. Inhibition of signaling between human CXCR4 and zebrafish ligands by the small molecule IT1t impairs the formation of triple-negative breast cancer early metastases in a zebrafish xenograft model. Dis Model Mech. 2016;9:141-53 pubmed publisher
    ..In conclusion, we confirm the validity of the zebrafish as a xenotransplantation model and propose a pharmacological approach to target CXCR4 in TNBC. ..
  13. Zakrzewska A, Cui C, Stockhammer O, Benard E, Spaink H, Meijer A. Macrophage-specific gene functions in Spi1-directed innate immunity. Blood. 2010;116:e1-11 pubmed publisher
    ..Therefore, based on our combined transcriptome analyses, we discovered novel early macrophage-specific marker genes, including a signal transducer pivotal for macrophage migration in the innate immune response. ..
  14. Ott E, Van Den Akker N, Sakalis P, Gittenberger de Groot A, te Velthuis A, Bagowski C. The lim domain only protein 7 is important in zebrafish heart development. Dev Dyn. 2008;237:3940-52 pubmed publisher
    ..We reveal the temporal and spatial expression patterns of this important gene during mouse, chicken and fish development and our findings suggest essential functions for LMO7 during vertebrate heart development. ..
  15. Zhang L, Huang H, Zhou F, Schimmel J, Pardo C, Zhang T, et al. RNF12 controls embryonic stem cell fate and morphogenesis in zebrafish embryos by targeting Smad7 for degradation. Mol Cell. 2012;46:650-61 pubmed publisher
    ..The gastrulation defects induced by ectopic and depleted Smad7 were rescued in part by RNF12 gain and loss of function, respectively. These findings demonstrate that RNF12 plays a critical role in TGF-? family signaling. ..
  16. Henkel C, Dirks R, Jansen H, Forlenza M, Wiegertjes G, Howe K, et al. Comparison of the exomes of common carp (Cyprinus carpio) and zebrafish (Danio rerio). Zebrafish. 2012;9:59-67 pubmed publisher
    ..This supports the hypothesis of an additional genome duplication event in the carp evolutionary history, which--due to a higher degree of compactness--did not result in a genome larger than that of zebrafish. ..
  17. de Andrea C, Prins F, Wiweger M, Hogendoorn P. Growth plate regulation and osteochondroma formation: insights from tracing proteoglycans in zebrafish models and human cartilage. J Pathol. 2011;224:160-8 pubmed publisher
    ..g., lack of PEI gradients). The cells in the reduced PEI-stained areas are likely associated with loss-of-function mutations in the EXT genes, and they might contribute to tumour initiation by disrupting the gradients...
  18. van der Vaart M, van Soest J, Spaink H, Meijer A. Functional analysis of a zebrafish myd88 mutant identifies key transcriptional components of the innate immune system. Dis Model Mech. 2013;6:841-54 pubmed publisher
    ..This zebrafish myd88 mutant will be a valuable tool for further study of the role of IL1R and TLR signaling in the innate immunity processes underlying infectious diseases, inflammatory disorders and cancer...
  19. Marques I, Weiss F, Vlecken D, Nitsche C, Bakkers J, Lagendijk A, et al. Metastatic behaviour of primary human tumours in a zebrafish xenotransplantation model. BMC Cancer. 2009;9:128 pubmed publisher
    ..Our results show that the zebrafish is a useful in vivo animal model for rapid analysis of invasion and metastatic behaviour of primary human tumour specimen. ..
  20. Zhou F, Xie F, Jin K, Zhang Z, Clerici M, Gao R, et al. USP4 inhibits SMAD4 monoubiquitination and promotes activin and BMP signaling. EMBO J. 2017;36:1623-1639 pubmed publisher
    ..Moreover, zebrafish depleted of USP4 exhibited defective cell migration and slower coordinated cell movement known as epiboly, both of which could be rescued by SMAD4. Therefore, USP4 is a critical determinant of SMAD4 activity. ..