Experts and Doctors on sperm capacitation in Utrecht, Netherlands


Locale: Utrecht, Netherlands
Topic: sperm capacitation

Top Publications

  1. de Vries K, Wiedmer T, Sims P, Gadella B. Caspase-independent exposure of aminophospholipids and tyrosine phosphorylation in bicarbonate responsive human sperm cells. Biol Reprod. 2003;68:2122-34 pubmed
    ..The proportion of live cells with PL scrambling corresponded with that showing capacitation-specific chlortetracyclin staining. ..
  2. Gadella B, Harrison R. The capacitating agent bicarbonate induces protein kinase A-dependent changes in phospholipid transbilayer behavior in the sperm plasma membrane. Development. 2000;127:2407-20 pubmed
    ..We suspect that the changes result from an activation of the non- specific bidirectional translocase ('scramblase'). They have important implications with respect to sperm fertilizing function. ..
  3. Gadella B. Sperm membrane physiology and relevance for fertilization. Anim Reprod Sci. 2008;107:229-36 pubmed publisher
    ..This paper emphasizes biochemical models for membrane surface modelling and their potential involvement for remodelling the sperm surface in the above described processes. ..
  4. Leemans B, Gadella B, Stout T, Sostaric E, De Schauwer C, Nelis H, et al. Combined albumin and bicarbonate induces head-to-head sperm agglutination which physically prevents equine sperm-oviduct binding. Reproduction. 2016;151:313-30 pubmed publisher
    ..In conclusion, a combination of albumin and HCO3(-) markedly induced sperm head-to-head agglutination which physically prevented stallion sperm to bind to oviduct epithelium. ..
  5. Flesch F, Wijnand E, van de Lest C, Colenbrander B, van Golde L, Gadella B. Capacitation dependent activation of tyrosine phosphorylation generates two sperm head plasma membrane proteins with high primary binding affinity for the zona pellucida. Mol Reprod Dev. 2001;60:107-15 pubmed
    ..Plasma membrane proteins from freshly ejaculated sperm cells did not exhibit any zona pellucida binding proteins, likely because these proteins were not tyrosine phosphorylated. ..
  6. Flesch F, Brouwers J, Nievelstein P, Verkleij A, van Golde L, Colenbrander B, et al. Bicarbonate stimulated phospholipid scrambling induces cholesterol redistribution and enables cholesterol depletion in the sperm plasma membrane. J Cell Sci. 2001;114:3543-55 pubmed
    ..A model is proposed in which phospholipid scrambling induces the formation of an apical membrane raft in the sperm head surface that enables albumin mediated efflux of cholesterol. ..
  7. Sostaric E, Dieleman S, van de Lest C, Colenbrander B, Vos P, Garcia Gil N, et al. Sperm binding properties and secretory activity of the bovine oviduct immediately before and after ovulation. Mol Reprod Dev. 2008;75:60-74 pubmed
    ..This indicates, that systemic endocrine changes around ovulation rather than specific oviduct changes at the ipsi-lateral oviduct induce secretion in oviduct epithelial cells, and thus induce sperm release. ..
  8. Sostaric E, Aalberts M, Gadella B, Stout T. The roles of the epididymis and prostasomes in the attainment of fertilizing capacity by stallion sperm. Anim Reprod Sci. 2008;107:237-48 pubmed publisher
    ..This article reviews current information on the roles of epididymal and accessory gland fluids on the acquisition of fertilizing capacity by stallion sperm. ..
  9. Gadella B. The assembly of a zona pellucida binding protein complex in sperm. Reprod Domest Anim. 2008;43 Suppl 5:12-9 pubmed publisher
    ..The relevance for this to predict and identify the proteins involved in sperm zona binding in vivo is discussed...

More Information


  1. Tsai P, Garcia Gil N, van Haeften T, Gadella B. How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane. PLoS ONE. 2010;5:e11204 pubmed publisher
    ..Thus, sperm can be considered a valuable model for studying exocytosis...
  2. Tsai P, Brewis I, van Maaren J, Gadella B. Involvement of complexin 2 in docking, locking and unlocking of different SNARE complexes during sperm capacitation and induced acrosomal exocytosis. PLoS ONE. 2012;7:e32603 pubmed publisher
    ..The possibility to study separate and dynamic interactions between SNARE proteins, complexin and Ca(2+) which are all involved in AE make sperm an ideal model for studying exocytosis...