Genomes and Genes
Experts and Doctors on caenorhabditis elegans in Utrecht, Netherlands
Locale: Utrecht, Netherlands
Topic: caenorhabditis elegans
- Kamminga L, Van Wolfswinkel J, Luteijn M, Kaaij L, Bagijn M, Sapetschnig A, et al. Differential impact of the HEN1 homolog HENN-1 on 21U and 26G RNAs in the germline of Caenorhabditis elegans. PLoS Genet. 2012;8:e1002702 pubmed publisher..These studies further refine our understanding of endogenous RNAi in C. elegans and the roles for Hen1 like enzymes in these pathways. ..
- Van Wolfswinkel J, Claycomb J, Batista P, Mello C, Berezikov E, Ketting R. CDE-1 affects chromosome segregation through uridylation of CSR-1-bound siRNAs. Cell. 2009;139:135-48 pubmed publisher..The conserved nature of CDE-1 suggests that similar sorting mechanisms may operate in other animals, including mammals. ..
- Yang P, Lorenowicz M, Silhankova M, Coudreuse D, Betist M, Korswagen H. Wnt signaling requires retromer-dependent recycling of MIG-14/Wntless in Wnt-producing cells. Dev Cell. 2008;14:140-7 pubmed..We propose that MIG-14/Wls cycles between the Golgi and the plasma membrane to mediate Wnt secretion. Regulation of this transport pathway may enable Wnt-producing cells to control the range of Wnt signaling in the tissue. ..
- Tops B, Plasterk R, Ketting R. The Caenorhabditis elegans Argonautes ALG-1 and ALG-2: almost identical yet different. Cold Spring Harb Symp Quant Biol. 2006;71:189-94 pubmed..Here, we present genetic and biochemical data that hint at individual nonredundant functions for ALG-1 and ALG-2 in the processing of precursor miRNAs to mature miRNAs. ..
- Middelkoop T, Williams L, Yang P, Luchtenberg J, Betist M, Ji N, et al. The thrombospondin repeat containing protein MIG-21 controls a left-right asymmetric Wnt signaling response in migrating C. elegans neuroblasts. Dev Biol. 2012;361:338-48 pubmed publisher..We conclude that MIG-21 and UNC-40 control the asymmetry in Wnt signaling response by restricting posterior polarization to one of the two Q neuroblasts. ..
- Harterink M, Port F, Lorenowicz M, McGough I, Silhankova M, Betist M, et al. A SNX3-dependent retromer pathway mediates retrograde transport of the Wnt sorting receptor Wntless and is required for Wnt secretion. Nat Cell Biol. 2011;13:914-923 pubmed publisher..These results demonstrate that SNX3 is part of an alternative retromer pathway that functionally separates the retrograde transport of Wls from other retromer cargo. ..
- Frische E, Pellis van Berkel W, van Haaften G, Cuppen E, Plasterk R, Tijsterman M, et al. RAP-1 and the RAL-1/exocyst pathway coordinate hypodermal cell organization in Caenorhabditis elegans. EMBO J. 2007;26:5083-92 pubmed..Genetic interactions show that the RAP-1 and RAL-1/exocyst pathway also act in parallel during larval stages. Together these data provide in vivo evidence for the exocyst complex as a downstream RAL-1 effector in cell migration. ..
- Stout G, Stigter E, Essers P, Mulder K, Kolkman A, Snijders D, et al. Insulin/IGF-1-mediated longevity is marked by reduced protein metabolism. Mol Syst Biol. 2013;9:679 pubmed publisher..elegans lifespan confirming the importance of these processes in Insulin/IGF-1-mediated longevity. Together, the results demonstrate a role for the metabolism of proteins in the Insulin/IGF-1-mediated extension of life. ..
- Ketting R, Fischer S, Bernstein E, Sijen T, Hannon G, Plasterk R. Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans. Genes Dev. 2001;15:2654-9 pubmed..dcr-1 mutants show a defect in RNAi. Furthermore, a combination of phenotypic abnormalities and RNA analysis suggests a role for dcr-1 in a regulatory pathway comprised of small temporal RNA (let-7) and its target (e.g., lin-41). ..
- Waaijers S, Portegijs V, Kerver J, Lemmens B, Tijsterman M, van den Heuvel S, et al. CRISPR/Cas9-targeted mutagenesis in Caenorhabditis elegans. Genetics. 2013;195:1187-91 pubmed publisher..Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at specific sites in the C. elegans genome. ..
- Van Der Linden A, Simmer F, Cuppen E, Plasterk R. The G-protein beta-subunit GPB-2 in Caenorhabditis elegans regulates the G(o)alpha-G(q)alpha signaling network through interactions with the regulator of G-protein signaling proteins EGL-10 and EAT-16. Genetics. 2001;158:221-35 pubmed..Taken together, our results suggest that GPB-2 works in concert with the RGS proteins EGL-10 and EAT-16 to regulate GOA-1 (G(o)alpha) and EGL-30 (G(q)alpha) signaling. ..
- Medema R, Kops G, Bos J, Burgering B. AFX-like Forkhead transcription factors mediate cell-cycle regulation by Ras and PKB through p27kip1. Nature. 2000;404:782-7 pubmed..We conclude that AFX-like proteins are involved in cell-cycle regulation and that inactivation of these proteins is an important step in oncogenic transformation. ..
- Coudreuse D, Roël G, Betist M, Destree O, Korswagen H. Wnt gradient formation requires retromer function in Wnt-producing cells. Science. 2006;312:921-4 pubmed..These results demonstrate that the ability of Wnt to regulate long-range patterning events is dependent on a critical and conserved function of the retromer complex within Wnt-producing cells. ..
- Simmer F, Moorman C, van der Linden A, Kuijk E, van den Berghe P, Kamath R, et al. Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions. PLoS Biol. 2003;1:E12 pubmed..elegans genome. The resulting dataset will be valuable in conjunction with other functional genomics approaches, as well as in other model organisms. ..
- Vastenhouw N, Fischer S, Robert V, Thijssen K, Fraser A, Kamath R, et al. A genome-wide screen identifies 27 genes involved in transposon silencing in C. elegans. Curr Biol. 2003;13:1311-6 pubmed..Since most of the newly identified genes have clear homologs in other species, and since transposons are found from protozoa to human, it seems likely that they also protect other genomes against transposon activity in the germline. ..
- Pothof J, van Haaften G, Thijssen K, Kamath R, Fraser A, Ahringer J, et al. Identification of genes that protect the C. elegans genome against mutations by genome-wide RNAi. Genes Dev. 2003;17:443-8 pubmed..Because known mutator genes are causally involved in many hereditary and sporadic human cancers, it is likely that some of these new mutators are equally relevant in cancer etiology. ..
- Tijsterman M, Okihara K, Thijssen K, Plasterk R. PPW-1, a PAZ/PIWI protein required for efficient germline RNAi, is defective in a natural isolate of C. elegans. Curr Biol. 2002;12:1535-40 pubmed..Our data indicate that at least two members of this family are required for complete and effective RNAi in C. elegans. ..
- Korzelius J, The I, Ruijtenberg S, Portegijs V, Xu H, Horvitz H, et al. C. elegans MCM-4 is a general DNA replication and checkpoint component with an epidermis-specific requirement for growth and viability. Dev Biol. 2011;350:358-69 pubmed publisher..Thus, C. elegans mcm-4 has conserved functions in DNA replication and replication checkpoint control but also shows unexpected tissue-specific requirements. ..
- Consonni S, Maurice M, Bos J. DEP domains: structurally similar but functionally different. Nat Rev Mol Cell Biol. 2014;15:357-62 pubmed publisher..DEP domains can interact with various partners at the membrane, including phospholipids and membrane receptors, and their binding is subject to regulation. ..
- Silhankova M, Port F, Harterink M, Basler K, Korswagen H. Wnt signalling requires MTM-6 and MTM-9 myotubularin lipid-phosphatase function in Wnt-producing cells. EMBO J. 2010;29:4094-105 pubmed publisher..We conclude that regulation of endosomal trafficking by the MTM-6/MTM-9 myotubularin complex is required for the retromer-dependent recycling of MIG-14/Wls and Wnt secretion. ..
- Simmer F, Tijsterman M, Parrish S, Koushika S, Nonet M, Fire A, et al. Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi. Curr Biol. 2002;12:1317-9 pubmed..The rrf-3 strain can be useful to study genes that, in wild-type, do not show a phenotype after RNAi, and it is probably the strain of choice for genome-wide RNAi screens. ..
- van der Voet M, Lorson M, Srinivasan D, Bennett K, van den Heuvel S. C. elegans mitotic cyclins have distinct as well as overlapping functions in chromosome segregation. Cell Cycle. 2009;8:4091-102 pubmed
- Steiner F, Okihara K, Hoogstrate S, Sijen T, Ketting R. RDE-1 slicer activity is required only for passenger-strand cleavage during RNAi in Caenorhabditis elegans. Nat Struct Mol Biol. 2009;16:207-11 pubmed publisher..These results uncouple the role of the RDE-1 RNase H activity in small RNA maturation from its role in target-mRNA silencing in vivo. ..
- Oosterveen T, Coudreuse D, Yang P, Fraser E, Bergsma J, Dale T, et al. Two functionally distinct Axin-like proteins regulate canonical Wnt signaling in C. elegans. Dev Biol. 2007;308:438-48 pubmed..We conclude that Axin function in C. elegans is divided over two different Axin orthologs that have specific functions in negatively regulating canonical Wnt signaling. ..
- van den Berg M, van Gogh I, Smits A, van Triest M, Dansen T, Visscher M, et al. The small GTPase RALA controls c-Jun N-terminal kinase-mediated FOXO activation by regulation of a JIP1 scaffold complex. J Biol Chem. 2013;288:21729-41 pubmed publisher..Furthermore, regulation of FOXO by RALA and JIP1 is conserved in C. elegans, where both ral-1 and jip-1 depletion impairs heat shock-induced nuclear translocation of the FOXO orthologue DAF16. ..
- Soete G, Betist M, Korswagen H. Regulation of Caenorhabditis elegans body size and male tail development by the novel gene lon-8. BMC Dev Biol. 2007;7:20 pubmed..The novel gene lon-8 encodes a secreted product of the hypodermis that controls body size and male ray morphology in C. elegans. lon-8 genetically interacts with enzymes that affect the composition of the cuticle. ..
- van Haaften G, Romeijn R, Pothof J, Koole W, Mullenders L, Pastink A, et al. Identification of conserved pathways of DNA-damage response and radiation protection by genome-wide RNAi. Curr Biol. 2006;16:1344-50 pubmed
- Tijsterman M, Pothof J, Plasterk R. Frequent germline mutations and somatic repeat instability in DNA mismatch-repair-deficient Caenorhabditis elegans. Genetics. 2002;161:651-60 pubmed..elegans as a model system to discover genes involved in maintaining DNA stability by large-scale RNAi screens. ..
- de Vet E, van den Bosch H. Alkyl-dihydroxyacetonephosphate synthase. Cell Biochem Biophys. 2000;32 Spring:117-21 pubmed..A clone encoding a Caenorhabditis elegans homolog of the mammalian enzymes was characterized. In contrast to the mammalian enzymes, this C. elegans enzyme lacks a N-terminal PTS type 2 motif, but carries a C-terminal PTS type 1. ..