Experts and Doctors on bicarbonates in Utrecht, Netherlands
Locale: Utrecht, Netherlands
Gadella B, Harrison R. The capacitating agent bicarbonate induces protein kinase A-dependent changes in phospholipid transbilayer behavior in the sperm plasma membrane. Development. 2000;127:2407-20 pubmed
..We suspect that the changes result from an activation of the non- specific bidirectional translocase ('scramblase'). They have important implications with respect to sperm fertilizing function. ..
de Vries K, Wiedmer T, Sims P, Gadella B. Caspase-independent exposure of aminophospholipids and tyrosine phosphorylation in bicarbonate responsive human sperm cells. Biol Reprod. 2003;68:2122-34 pubmed
..The proportion of live cells with PL scrambling corresponded with that showing capacitation-specific chlortetracyclin staining. ..
Leemans B, Gadella B, Stout T, Sostaric E, De Schauwer C, Nelis H, et al
. Combined albumin and bicarbonate induces head-to-head sperm agglutination which physically prevents equine sperm-oviduct binding. Reproduction. 2016;151:313-30 pubmed publisher
..In conclusion, a combination of albumin and HCO3(-) markedly induced sperm head-to-head agglutination which physically prevented stallion sperm to bind to oviduct epithelium. ..
Flesch F, Brouwers J, Nievelstein P, Verkleij A, van Golde L, Colenbrander B, et al
. Bicarbonate stimulated phospholipid scrambling induces cholesterol redistribution and enables cholesterol depletion in the sperm plasma membrane. J Cell Sci. 2001;114:3543-55 pubmed
..A model is proposed in which phospholipid scrambling induces the formation of an apical membrane raft in the sperm head surface that enables albumin mediated efflux of cholesterol. ..
Tsai P, Brewis I, van Maaren J, Gadella B. Involvement of complexin 2 in docking, locking and unlocking of different SNARE complexes during sperm capacitation and induced acrosomal exocytosis. PLoS ONE. 2012;7:e32603 pubmed publisher
..The possibility to study separate and dynamic interactions between SNARE proteins, complexin and Ca(2+) which are all involved in AE make sperm an ideal model for studying exocytosis...