Experts and Doctors on saccharomyces cerevisiae in Suita, Ōsaka, Japan


Locale: Suita, Ōsaka, Japan
Topic: saccharomyces cerevisiae

Top Publications

  1. Kawasaki Y, Hiraga S, Sugino A. Interactions between Mcm10p and other replication factors are required for proper initiation and elongation of chromosomal DNA replication in Saccharomyces cerevisiae. Genes Cells. 2000;5:975-89 pubmed
  2. Maeda Y, Watanabe R, Harris C, Hong Y, Ohishi K, Kinoshita K, et al. PIG-M transfers the first mannose to glycosylphosphatidylinositol on the lumenal side of the ER. EMBO J. 2001;20:250-61 pubmed
  3. Kondo Okamoto N, Noda N, Suzuki S, Nakatogawa H, Takahashi I, Matsunami M, et al. Autophagy-related protein 32 acts as autophagic degron and directly initiates mitophagy. J Biol Chem. 2012;287:10631-8 pubmed publisher
    ..Taken together, our data implicate Atg32 as a bipartite platform recruiting Atg8 and Atg11 to the mitochondrial surface and forming an initiator complex crucial for mitophagy. ..
  4. Akaboshi E, Inoue Y, Ryo H. Cloning of the cDNA and genomic DNA that correspond to the recA-like gene of Drosophila melanogaster. Jpn J Genet. 1994;69:663-70 pubmed
    ..By in situ hybridization to the salivary gland chromosomes, the recA-like gene was cytologically mapped to 99D of the third chromosome. ..
  5. Zhu Z, Mori S, Oshiumi H, Matsuzaki K, Shinohara M, Shinohara A. Cyclin-dependent kinase promotes formation of the synaptonemal complex in yeast meiosis. Genes Cells. 2010;15:1036-50 pubmed publisher
    ..Compromised CDK activity in meiotic prophase leads to defective SC formation without affecting DSB formation. These results suggest that CDK-dependent phosphorylation regulates meiotic chromosome morphogenesis...
  6. Leem S, Chung C, Sunwoo Y, Araki H. Meiotic role of SWI6 in Saccharomyces cerevisiae. Nucleic Acids Res. 1998;26:3154-8 pubmed
    ..These results suggest that SWI6 enhances the expression level of the recombination genes in meiosis in a dosage-dependent manner, which results in an effect on the frequency of meiotic recombination. ..
  7. Matsuzaki K, Shinohara A, Shinohara M. Forkhead-associated domain of yeast Xrs2, a homolog of human Nbs1, promotes nonhomologous end joining through interaction with a ligase IV partner protein, Lif1. Genetics. 2008;179:213-25 pubmed publisher
    ..The interaction between Xrs2 and Lif1 through the FHA domain is conserved in humans; the FHA domain Nbs1 interacts with Xrcc4, a Lif1 homolog of human. ..
  8. Auesukaree C, Tochio H, Shirakawa M, Kaneko Y, Harashima S. Plc1p, Arg82p, and Kcs1p, enzymes involved in inositol pyrophosphate synthesis, are essential for phosphate regulation and polyphosphate accumulation in Saccharomyces cerevisiae. J Biol Chem. 2005;280:25127-33 pubmed
  9. Ohya T, Maki S, Kawasaki Y, Sugino A. Structure and function of the fourth subunit (Dpb4p) of DNA polymerase epsilon in Saccharomyces cerevisiae. Nucleic Acids Res. 2000;28:3846-52 pubmed
    ..cerevisiae, even if it is not essential for cell growth. Structural homologues of DPB4 are present in other eukaryotic genomes, suggesting that the complex structure of S. cerevisiae Polepsilon is conserved in eukaryotes. ..

More Information


  1. Takeshita K, Sakata S, Yamashita E, Fujiwara Y, Kawanabe A, Kurokawa T, et al. X-ray crystal structure of voltage-gated proton channel. Nat Struct Mol Biol. 2014;21:352-7 pubmed publisher
    ..These findings provide a platform for understanding the general principles of voltage sensing and proton permeation. ..
  2. Laviña W, Shahsavarani H, Saidi A, Sugiyama M, Kaneko Y, Harashima S. Suppression mechanism of the calcium sensitivity in Saccharomyces cerevisiae ptp2Δmsg5Δ double disruptant involves a novel HOG-independent function of Ssk2, transcription factor Msn2 and the protein kinase A component Bcy1. J Biosci Bioeng. 2014;117:135-141 pubmed publisher
  3. Hiraga S, Hagihara Hayashi A, Ohya T, Sugino A. DNA polymerases alpha, delta, and epsilon localize and function together at replication forks in Saccharomyces cerevisiae. Genes Cells. 2005;10:297-309 pubmed
    ..These data collectively suggest that bidirectional replication occurs at specific foci in yeast chromosomes and that pol alpha, -delta, and -epsilon localize and function together at multiple replication forks during S phase. ..
  4. Komori K, Sakae S, Shinagawa H, Morikawa K, Ishino Y. A Holliday junction resolvase from Pyrococcus furiosus: functional similarity to Escherichia coli RuvC provides evidence for conserved mechanism of homologous recombination in Bacteria, Eukarya, and Archaea. Proc Natl Acad Sci U S A. 1999;96:8873-8 pubmed
    ..These results support the notion that the formation and resolution of Holliday junction is the common mechanism of homologous recombination in the three domains of life. ..
  5. Shimizu K, Kawasaki Y, Hiraga S, Tawaramoto M, Nakashima N, Sugino A. The fifth essential DNA polymerase phi in Saccharomyces cerevisiae is localized to the nucleolus and plays an important role in synthesis of rRNA. Proc Natl Acad Sci U S A. 2002;99:9133-8 pubmed
    ..Pol5p is localized exclusively to the nucleolus and binds near or at the enhancer region of rRNA-encoding DNA repeating units. ..
  6. Hermansyah -, Laviña W, Sugiyama M, Kaneko Y, Harashima S. Identification of protein kinase disruptions as suppressors of the calcium sensitivity of S. cerevisiae Deltaptp2 Deltamsg5 protein phosphatase double disruptant. Arch Microbiol. 2010;192:157-65 pubmed publisher
  7. Taniura H, Matsumoto K, Yoshikawa K. Physical and functional interactions of neuronal growth suppressor necdin with p53. J Biol Chem. 1999;274:16242-8 pubmed
    ..On the other hand, necdin inhibited p53-induced apoptosis of osteosarcoma U2OS cells. Thus, necdin can be a growth suppressor that targets p53 and modulates its biological functions in postmitotic neurons. ..
  8. Umikawa M, Tanaka K, Kamei T, Shimizu K, Imamura H, Sasaki T, et al. Interaction of Rho1p target Bni1p with F-actin-binding elongation factor 1alpha: implication in Rho1p-regulated reorganization of the actin cytoskeleton in Saccharomyces cerevisiae. Oncogene. 1998;16:2011-6 pubmed
    ..These results suggest that the Rho1p-Bni1p system regulates reorganization of the actin cytoskeleton through the interaction with both EF1alpha and profilin. ..
  9. Izawa R, Onoue T, Furukawa N, Mima J. Distinct contributions of vacuolar Qabc- and R-SNARE proteins to membrane fusion specificity. J Biol Chem. 2012;287:3445-53 pubmed publisher
    ..Thus, our current study establishes that an appropriate assembly of Qabc-SNAREs is crucial for regulating fusion specificity, whereas R-SNARE itself has little contribution to specificity. ..
  10. Laviña W, Hermansyah -, Sugiyama M, Kaneko Y, Harashima S. Functionally redundant protein phosphatase genes PTP2 and MSG5 co-regulate the calcium signaling pathway in Saccharomyces cerevisiae upon exposure to high extracellular calcium concentration. J Biosci Bioeng. 2013;115:138-46 pubmed publisher
    ..Also, we show that PTP2 and MSG5 are key regulatory PPases that prevent over-activation of the calcium-induced signaling cascade under the parallel control of the SLT2 and calcineurin pathways. ..
  11. Sugino A, Ohara T, Sebastian J, Nakashima N, Araki H. DNA polymerase epsilon encoded by cdc20+ is required for chromosomal DNA replication in the fission yeast Schizosaccharomyces pombe. Genes Cells. 1998;3:99-110 pubmed
    ..These data strongly suggest that Pol epsilon is required for normal chromosomal DNA replication in S. pombe, as is PolII in S. cerevisiae. Thus, eukaryotic chromosomal DNA is replicated differently from that of viral SV40 DNA. ..
  12. Ohya T, Kawasaki Y, Hiraga S, Kanbara S, Nakajo K, Nakashima N, et al. The DNA polymerase domain of pol(epsilon) is required for rapid, efficient, and highly accurate chromosomal DNA replication, telomere length maintenance, and normal cell senescence in Saccharomyces cerevisiae. J Biol Chem. 2002;277:28099-108 pubmed
    ..These results indicate that the DNA polymerase domain of Pol2p is required for rapid, efficient, and highly accurate chromosomal DNA replication in yeast. ..
  13. Hiyama H, Yokoi M, Masutani C, Sugasawa K, Maekawa T, Tanaka K, et al. Interaction of hHR23 with S5a. The ubiquitin-like domain of hHR23 mediates interaction with S5a subunit of 26 S proteasome. J Biol Chem. 1999;274:28019-25 pubmed
    ..From these results, we propose that part of hHR23 proteins are involved in the proteolytic pathway in cells. ..
  14. Sugiyama M, Nugroho S, Iida N, Sakai T, Kaneko Y, Harashima S. Genetic interactions of ribosome maturation factors Yvh1 and Mrt4 influence mRNA decay, glycogen accumulation, and the expression of early meiotic genes in Saccharomyces cerevisiae. J Biochem. 2011;150:103-11 pubmed publisher
    ..Since a defect in ribosome biogenesis in general can be related to other various defects, the ribosome biogenesis defect caused by absence of Yvh1 might be an indirect cause of observed phenotypes. ..
  15. Ohishi K, Inoue N, Kinoshita T. PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8. EMBO J. 2001;20:4088-98 pubmed
    ..Saccharomyces cerevisiae Gpi16p (YHR188C) and Gpi17p (YDR434W) are orthologues of PIG-T and PIG-S, respectively. ..
  16. Osada S, Kurita M, Nishikawa J, Nishihara T. Chromatin assembly factor Asf1p-dependent occupancy of the SAS histone acetyltransferase complex at the silent mating-type locus HMLalpha. Nucleic Acids Res. 2005;33:2742-50 pubmed
    ..These data suggest that the HAT activity and ASF1-dependent localization of the SAS complex are required for SIR1-dependent HMLalpha silencing. ..
  17. Nonaka H, Tanaka K, Hirano H, Fujiwara T, Kohno H, Umikawa M, et al. A downstream target of RHO1 small GTP-binding protein is PKC1, a homolog of protein kinase C, which leads to activation of the MAP kinase cascade in Saccharomyces cerevisiae. EMBO J. 1995;14:5931-8 pubmed
    ..These results indicate that there are at least two signaling pathways regulated by Rho1p and that one of the downstream targets is Pkc1p, leading to the activation of the MAP kinase cascade. ..
  18. Gothwal S, Patel N, Colletti M, Sasanuma H, Shinohara M, Hochwagen A, et al. The Double-Strand Break Landscape of Meiotic Chromosomes Is Shaped by the Paf1 Transcription Elongation Complex in Saccharomyces cerevisiae. Genetics. 2016;202:497-512 pubmed publisher
    ..These observations suggest that Rtf1, and by extension the Paf1C, modulate the genomic DSB landscape independently of H3K4 methylation. ..
  19. Yabuta N, Kajimura N, Mayanagi K, Sato M, Gotow T, Uchiyama Y, et al. Mammalian Mcm2/4/6/7 complex forms a toroidal structure. Genes Cells. 2003;8:413-21 pubmed
    ..The Mcm2/4/6/7 tetramer forms a toroidal structure that is distinct from that of the Mcm4/6/7 hexamer in size and shape. ..
  20. Ogi H, Wang C, Nakai W, Kawasaki Y, Masumoto H. The role of the Saccharomyces cerevisiae Cdc7-Dbf4 complex in the replication checkpoint. Gene. 2008;414:32-40 pubmed publisher
    ..These results suggest that Cdc7-Dbf4 is required for full activation of Rad53 in response to replication stress. ..
  21. Ohashi Kobayashi A, Ohashi K, Du W, Omote H, Nakamoto R, Al Shawi M, et al. Examination of drug resistance activity of human TAP-like (ABCB9) expressed in yeast. Biochem Biophys Res Commun. 2006;343:597-601 pubmed
    ..These findings suggest that the transport activity of hTAPL is important for conferring high valinomycin-sensitive phenotype to yeast...
  22. Kawasaki Y, Kim H, Kojima A, Seki T, Sugino A. Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro. Genes Cells. 2006;11:745-56 pubmed
  23. Hirasawa T, Ashitani K, Yoshikawa K, Nagahisa K, Furusawa C, Katakura Y, et al. Comparison of transcriptional responses to osmotic stresses induced by NaCl and sorbitol additions in Saccharomyces cerevisiae using DNA microarray. J Biosci Bioeng. 2006;102:568-71 pubmed
    ..Our results suggest that the difference in the transcriptional responses of the two strains between NaCl and sorbitol additions is small when the dynamics of the total change in gene expression are similar...
  24. Kinoshita M, Kamagata K, Maeda A, Goto Y, Komatsuzaki T, Takahashi S. Development of a technique for the investigation of folding dynamics of single proteins for extended time periods. Proc Natl Acad Sci U S A. 2007;104:10453-8 pubmed
    ..The technique was expected to reveal dynamics of proteins along the folding processes without artifacts caused by immobilization. ..
  25. Shingai T, Ikeda W, Kakunaga S, Morimoto K, Takekuni K, Itoh S, et al. Implications of nectin-like molecule-2/IGSF4/RA175/SgIGSF/TSLC1/SynCAM1 in cell-cell adhesion and transmembrane protein localization in epithelial cells. J Biol Chem. 2003;278:35421-7 pubmed
    ..These results indicate the unique localization of Necl-2 and its possible involvement in localization of a transmembrane protein(s) through Pals2. ..
  26. Kosaka H, Shinohara M, Shinohara A. Csm4-dependent telomere movement on nuclear envelope promotes meiotic recombination. PLoS Genet. 2008;4:e1000196 pubmed publisher
    ..We propose that chromosome movement and associated telomere dynamics at the nuclear envelope promotes the completion of key biochemical steps during meiotic recombination. ..
  27. Shinohara Y, Koyama Y, Ukai Tadenuma M, Hirokawa T, Kikuchi M, Yamada R, et al. Temperature-Sensitive Substrate and Product Binding Underlie Temperature-Compensated Phosphorylation in the Clock. Mol Cell. 2017;67:783-798.e20 pubmed publisher
    ..Surprisingly, this domain can confer temperature compensation on a temperature-sensitive TTBK1. These findings suggest the temperature-sensitive substrate- and product-binding mechanisms underlie temperature compensation. ..
  28. Watanabe R, Ohishi K, Maeda Y, Nakamura N, Kinoshita T. Mammalian PIG-L and its yeast homologue Gpi12p are N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential in glycosylphosphatidylinositol biosynthesis. Biochem J. 1999;339 ( Pt 1):185-92 pubmed
    ..The disruption of the gene caused lethality in S. cerevisiae. These results indicate that GlcNAc-PI de-N-acetylase is conserved between mammals and yeasts and that the de-N-acetylation step is also indispensable in yeasts. ..
  29. Masutani C, Kusumoto R, Yamada A, Dohmae N, Yokoi M, Yuasa M, et al. The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase eta. Nature. 1999;399:700-4 pubmed
    ..Together, these results indicate that DNA polymerase eta could be the XPV gene product. ..
  30. Hermansyah -, Sugiyama M, Kaneko Y, Harashima S. Yeast protein phosphatases Ptp2p and Msg5p are involved in G1-S transition, CLN2 transcription, and vacuole morphogenesis. Arch Microbiol. 2009;191:721-33 pubmed publisher
    ..These findings suggest that both Ptp2p and Msg5p are involved in the G1 to S transition and vacuole morphogenesis possibly through their regulation of Slt2 pathway. ..
  31. Fujita Y, Sasaki T, Fukui K, Kotani H, Kimura T, Hata Y, et al. Phosphorylation of Munc-18/n-Sec1/rbSec1 by protein kinase C: its implication in regulating the interaction of Munc-18/n-Sec1/rbSec1 with syntaxin. J Biol Chem. 1996;271:7265-8 pubmed
  32. Usui T, Kanehara Y. Elevated Rad53 kinase activity influences formation and interhomolog repair of meiotic DNA double-strand breaks in budding yeast. Biochem Biophys Res Commun. 2013;441:593-9 pubmed publisher
    ..The overexpression led to partial activation of Rad53, uncovering that the negative impacts of Rad53 kinase activation on meiotic progression, and formation and interhomolog repair of meiotic programmed DSBs. ..
  33. Matsuda S, Okada N, Kodama T, Honda T, Iida T. A cytotoxic type III secretion effector of Vibrio parahaemolyticus targets vacuolar H+-ATPase subunit c and ruptures host cell lysosomes. PLoS Pathog. 2012;8:e1002803 pubmed publisher
    ..Therefore, our data suggest that the bacterial effector VepA targets subunit c of V-ATPase and induces the rupture of host cell lysosomes and subsequent cell death. ..
  34. Matsuzaki K, Terasawa M, Iwasaki D, Higashide M, Shinohara M. Cyclin-dependent kinase-dependent phosphorylation of Lif1 and Sae2 controls imprecise nonhomologous end joining accompanied by double-strand break resection. Genes Cells. 2012;17:473-93 pubmed publisher
    ..CDK-dependent modification of the NHEJ pathway might make DSB ends compatible for NHEJ and thus prevent competition between HR and NHEJ in hierarchy on the choice of DSB repair pathways. ..
  35. Rao H, Shinohara M, Shinohara A. Mps3 SUN domain is important for chromosome motion and juxtaposition of homologous chromosomes during meiosis. Genes Cells. 2011;16:1081-96 pubmed publisher
    ..These findings suggest that in meiosis, the Mps3 SUN domain is important for modulating chromosome motion events that act in meiotic chromosome juxtaposition and by extension, promoting proper morphogenesis of the synaptonemal complex. ..
  36. Inoue Y, Kawai Noma S, Koike Takeshita A, Taguchi H, Yoshida M. Yeast prion protein New1 can break Sup35 amyloid fibrils into fragments in an ATP-dependent manner. Genes Cells. 2011;16:545-56 pubmed publisher
    ..Thus, New1 potentially has a regulatory role in prion state in yeast, working independently of the Hsp104 system. ..
  37. Hirasaki M, Horiguchi M, Numamoto M, Sugiyama M, Kaneko Y, Nogi Y, et al. Saccharomyces cerevisiae protein phosphatase Ppz1 and protein kinases Sat4 and Hal5 are involved in the control of subcellular localization of Gln3 by likely regulating its phosphorylation state. J Biosci Bioeng. 2011;111:249-54 pubmed publisher
    ..From these observations, we conclude that Ppz1 controls Gln3 localization by regulating its phosphorylation state in combination with Sat4 and Hal5. ..
  38. Fujiwara T, Tanaka K, Mino A, Kikyo M, Takahashi K, Shimizu K, et al. Rho1p-Bni1p-Spa2p interactions: implication in localization of Bni1p at the bud site and regulation of the actin cytoskeleton in Saccharomyces cerevisiae. Mol Biol Cell. 1998;9:1221-33 pubmed
    ..These results indicate that both Rho1p and Spa2p are involved in the localization of Bni1p at the growth sites where Rho1p regulates reorganization of the actin cytoskeleton through Bni1p. ..
  39. Noskov V, Araki H, Sugino A. The RFC2 gene, encoding the third-largest subunit of the replication factor C complex, is required for an S-phase checkpoint in Saccharomyces cerevisiae. Mol Cell Biol. 1998;18:4914-23 pubmed
    ..These results suggest that the RFC2 gene product interacts with the CDC44/RFC1 and RFC5 gene products in the RF-C complex and with both DNA polymerases delta and epsilon during chromosomal DNA replication. ..
  40. Auesukaree C, Homma T, Kaneko Y, Harashima S. Transcriptional regulation of phosphate-responsive genes in low-affinity phosphate-transporter-defective mutants in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2003;306:843-50 pubmed
  41. Sakumoto N, Matsuoka I, Mukai Y, Ogawa N, Kaneko Y, Harashima S. A series of double disruptants for protein phosphatase genes in Saccharomyces cerevisiae and their phenotypic analysis. Yeast. 2002;19:587-99 pubmed
    ..3 M CaCl(2). The synthetic phenotypes in the two latter cases where each of the PPases is categorized in a different phosphatase family led us to discuss the novel mechanism involved in the functional redundancy of the PPases. ..
  42. Nagamune K, Nozaki T, Maeda Y, Ohishi K, Fukuma T, Hara T, et al. Critical roles of glycosylphosphatidylinositol for Trypanosoma brucei. Proc Natl Acad Sci U S A. 2000;97:10336-41 pubmed
    ..Infectivity to tsetse flies is partially impaired, particularly in the early stage. Therefore, parasitespecific inhibition of GPI biosynthesis should be an effective chemotherapy target against African trypanosomiasis. ..
  43. Ozaki Kuroda K, Yamamoto Y, Nohara H, Kinoshita M, Fujiwara T, Irie K, et al. Dynamic localization and function of Bni1p at the sites of directed growth in Saccharomyces cerevisiae. Mol Cell Biol. 2001;21:827-39 pubmed
    ..These results indicate that Bni1p regulates polarized growth within the bud through its unique and dynamic pattern of localization, dependent on multiple factors, including Cdc42p, Spa2p, Bud6p, and the actin cytoskeleton. ..
  44. Kang J, Hong Y, Ashida H, Shishioh N, Murakami Y, Morita Y, et al. PIG-V involved in transferring the second mannose in glycosylphosphatidylinositol. J Biol Chem. 2005;280:9489-97 pubmed
    ..PIG-V has two functionally important conserved regions facing the ER lumen. Taken together, we suggest that PIG-V is the second mannosyltransferase in GPI anchor biosynthesis. ..
  45. Mukai Y, Matsuo E, Roth S, Harashima S. Conservation of histone binding and transcriptional repressor functions in a Schizosaccharomyces pombe Tup1p homolog. Mol Cell Biol. 1999;19:8461-8 pubmed
    ..pombe. Furthermore, Tup11p binds specifically to histones H3 and H4 in vitro, indicating that both the repression and histone binding functions of Tup1p-related proteins are conserved across species. ..
  46. Hayase A, Takagi M, Miyazaki T, Oshiumi H, Shinohara M, Shinohara A. A protein complex containing Mei5 and Sae3 promotes the assembly of the meiosis-specific RecA homolog Dmc1. Cell. 2004;119:927-40 pubmed
  47. Hotta K, Tanaka K, Mino A, Kohno H, Takai Y. Interaction of the Rho family small G proteins with kinectin, an anchoring protein of kinesin motor. Biochem Biophys Res Commun. 1996;225:69-74 pubmed
    ..This protein is kinectin, known to be a vesicle membrane anchoring protein of kinesin, which is an ATPase motor transporting vesicles along microtubules. ..
  48. Kohno H, Tanaka K, Mino A, Umikawa M, Imamura H, Fujiwara T, et al. Bni1p implicated in cytoskeletal control is a putative target of Rho1p small GTP binding protein in Saccharomyces cerevisiae. EMBO J. 1996;15:6060-8 pubmed
    ..Furthermore, this synthetic lethality was caused by the incapability of RhoA to activate Pkc1p, but not glucan synthase. These results suggest that Rho1p regulates cytoskeletal reorganization at least through Bni1p and Pkc1p. ..
  49. Ueta R, Fujiwara N, Iwai K, Yamaguchi Iwai Y. Iron-induced dissociation of the Aft1p transcriptional regulator from target gene promoters is an initial event in iron-dependent gene suppression. Mol Cell Biol. 2012;32:4998-5008 pubmed publisher
  50. Matsumura H, Kusaka N, Nakamura T, Tanaka N, Sagegami K, Uegaki K, et al. Crystal structure of the N-terminal domain of the yeast general corepressor Tup1p and its functional implications. J Biol Chem. 2012;287:26528-38 pubmed publisher
    ..This altered structure explains why the mutant cannot bind Cyc8p. The data presented herein highlight the importance of the architecture of the Tup1p N-terminal domain for self-association. ..
  51. Yamashita K, Shinohara M, Shinohara A. Rad6-Bre1-mediated histone H2B ubiquitylation modulates the formation of double-strand breaks during meiosis. Proc Natl Acad Sci U S A. 2004;101:11380-5 pubmed
    ..Histone tail modifications might play a role in DSB formation during meiosis. ..
  52. Iwahashi H, Eguchi Y, Yasuhara N, Hanafusa T, Matsuzawa Y, Tsujimoto Y. Synergistic anti-apoptotic activity between Bcl-2 and SMN implicated in spinal muscular atrophy. Nature. 1997;390:413-7 pubmed
    ..Our results indicate that an absent or decreased anti-apoptotic activity of SMN in concert with Bcl-2 underlies the pathogenesis of SMA. ..
  53. Hashimoto K, Nakashima N, Ohara T, Maki S, Sugino A. The second subunit of DNA polymerase III (delta) is encoded by the HYS2 gene in Saccharomyces cerevisiae. Nucleic Acids Res. 1998;26:477-85 pubmed
    ..cerevisiae and is required for highly processive DNA synthesis catalyzed by DNA polymerase III in the presence of PCNA, RF-A and RF-C. ..
  54. Kihara M, Nakai W, Asano S, Suzuki A, Kitada K, Kawasaki Y, et al. Characterization of the yeast Cdc7p/Dbf4p complex purified from insect cells. Its protein kinase activity is regulated by Rad53p. J Biol Chem. 2000;275:35051-62 pubmed
    ..This result suggests that Rad53p controls the initiation of chromosomal DNA replication by regulating the protein kinase activity associated with the Cdc7p/Dbf4p complex. ..
  55. Asakura T, Sasaki T, Nagano F, Satoh A, Obaishi H, Nishioka H, et al. Isolation and characterization of a novel actin filament-binding protein from Saccharomyces cerevisiae. Oncogene. 1998;16:121-30 pubmed
    ..These results indicate that ABP140 is not required for cell growth but may be involved in the reorganization of F-actin in the budding yeast. ..
  56. Sakumoto N, Yamashita H, Mukai Y, Kaneko Y, Harashima S. Dual-specificity protein phosphatase Yvh1p, which is required for vegetative growth and sporulation, interacts with yeast pescadillo homolog in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2001;289:608-15 pubmed
    ..These observations indicate that Yph1p plays a role in sporulation in addition to cell cycle progression, and is a candidate for a substrate or a regulatory subunit of Yvh1p. ..
  57. Yoshida R, Tamura T, Takaoka C, Harada K, Kobayashi A, Mukai Y, et al. Metabolomics-based systematic prediction of yeast lifespan and its application for semi-rational screening of ageing-related mutants. Aging Cell. 2010;9:616-25 pubmed publisher
    ..The model facilitated the identification of putative longevity mutants. This work represents a novel approach to evaluate and screen complicated and quantitative phenotype by means of metabolomics. ..
  58. Hishida T, Kubota Y, Carr A, Iwasaki H. RAD6-RAD18-RAD5-pathway-dependent tolerance to chronic low-dose ultraviolet light. Nature. 2009;457:612-5 pubmed publisher
    ..Thus, the error-free PRR pathway is specifically important during chronic low-dose ultraviolet exposure to prevent counter-productive DNA checkpoint activation and allow cells to proliferate normally. ..
  59. Jung J, Ishida K, Nishihara T. Anti-estrogenic activity of fifty chemicals evaluated by in vitro assays. Life Sci. 2004;74:3065-74 pubmed
    ..These findings suggested that three of fifty chemicals could inhibit estrogen activity by competitive binding with 17beta-estradiol to the estrogen receptor. ..
  60. Kamimura Y, Masumoto H, Sugino A, Araki H. Sld2, which interacts with Dpb11 in Saccharomyces cerevisiae, is required for chromosomal DNA replication. Mol Cell Biol. 1998;18:6102-9 pubmed
    ..These results strongly suggest the involvement of the Dpb11-Sld2 complex in a step close to the initiation of DNA replication. ..
  61. Koch G, Tanaka K, Masuda T, Yamochi W, Nonaka H, Takai Y. Association of the Rho family small GTP-binding proteins with Rho GDP dissociation inhibitor (Rho GDI) in Saccharomyces cerevisiae. Oncogene. 1997;15:417-22 pubmed
    ..These results indicate that yeast Rho GDI possesses properties similar to those of mammalian Rho GDI, and that there is a cytosolic factor which functionally substitutes for Rho GDI in yeast. ..
  62. Auesukaree C, Homma T, Tochio H, Shirakawa M, Kaneko Y, Harashima S. Intracellular phosphate serves as a signal for the regulation of the PHO pathway in Saccharomyces cerevisiae. J Biol Chem. 2004;279:17289-94 pubmed
  63. Hishida T, Ohya T, Kubota Y, Kamada Y, Shinagawa H. Functional and physical interaction of yeast Mgs1 with PCNA: impact on RAD6-dependent DNA damage tolerance. Mol Cell Biol. 2006;26:5509-17 pubmed
    ..The proposed roles for Mgs1, Srs2, and modified PCNA during replication arrest highlight the importance of modulating the RAD6 and RAD52 pathways to avoid genome instability. ..
  64. Kunoh T, Kaneko Y, Harashima S. YHP1 encodes a new homeoprotein that binds to the IME1 promoter in Saccharomyces cerevisiae. Yeast. 2000;16:439-49 pubmed
    ..We suggest that the homeoprotein Yhp1 that binds directly to the 28 bp region of the IME1 promoter is a new repressor acting under glucose growth conditions. ..
  65. Fujiwara T, Tanaka K, Inoue E, Kikyo M, Takai Y. Bni1p regulates microtubule-dependent nuclear migration through the actin cytoskeleton in Saccharomyces cerevisiae. Mol Cell Biol. 1999;19:8016-27 pubmed
    ..Bni1p lacking the Rho-binding region did not suppress the pac1 bni1 growth defect, suggesting a requirement for the Rho1p-Bni1p interaction in microtubule function. ..
  66. Magbanua J, Ogawa N, Harashima S, Oshima Y. The transcriptional activators of the PHO regulon, Pho4p and Pho2p, interact directly with each other and with components of the basal transcription machinery in Saccharomyces cerevisiae. J Biochem. 1997;121:1182-9 pubmed