Experts and Doctors on dna directed dna polymerase in Suita, Ōsaka, Japan


Locale: Suita, Ōsaka, Japan
Topic: dna directed dna polymerase

Top Publications

  1. Akagi J, Masutani C, Kataoka Y, Kan T, Ohashi E, Mori T, et al. Interaction with DNA polymerase eta is required for nuclear accumulation of REV1 and suppression of spontaneous mutations in human cells. DNA Repair (Amst). 2009;8:585-99 pubmed publisher
    ..Thus, Poleta-REV1 interactions prevent spontaneous mutations, probably by promoting accurate TLS past endogenous DNA lesions, while the interaction is dispensable for accurate Poleta-mediated TLS of UV-induced lesions. ..
  2. Yuasa M, Masutani C, Eki T, Hanaoka F. Genomic structure, chromosomal localization and identification of mutations in the xeroderma pigmentosum variant (XPV) gene. Oncogene. 2000;19:4721-8 pubmed
    ..These findings provide a basis for diagnosis and therapy of XP-V patients. ..
  3. Ishino S, Oyama T, Yuasa M, Morikawa K, Ishino Y. Mutational analysis of Pyrococcus furiosus replication factor C based on the three-dimensional structure. Extremophiles. 2003;7:169-75 pubmed
    ..These results contribute to our general understanding of the structure-function relationship of the RFC molecule for the clamp-loading event. ..
  4. Kanao R, Hanaoka F, Masutani C. A novel interaction between human DNA polymerase eta and MutLalpha. Biochem Biophys Res Commun. 2009;389:40-5 pubmed publisher
    ..MMR proteins were more abundant in the Poleta complex on the chromatin of S phase-synchronized cells than of asynchronous cells, suggesting that the interaction between Poleta and MLH1 is involved in DNA replication. ..
  5. Masutani C, Kusumoto R, Iwai S, Hanaoka F. Mechanisms of accurate translesion synthesis by human DNA polymerase eta. EMBO J. 2000;19:3100-9 pubmed
  6. Nishino T, Komori K, Tsuchiya D, Ishino Y, Morikawa K. Crystal structure and functional implications of Pyrococcus furiosus hef helicase domain involved in branched DNA processing. Structure. 2005;13:143-53 pubmed
    ..Hence, our findings provide a structural basis for the functional mechanisms of this helicase/nuclease family. ..
  7. Hashimoto H, Nishioka M, Fujiwara S, Takagi M, Imanaka T, Inoue T, et al. Crystal structure of DNA polymerase from hyperthermophilic archaeon Pyrococcus kodakaraensis KOD1. J Mol Biol. 2001;306:469-77 pubmed publisher
    ..The stabilization of the melted DNA structure at the forked-point may be correlated with the high PCR performance of KOD DNA polymerase, which is due to low error rate, high elongation rate and processivity...
  8. Bocquier A, Liu L, Cann I, Komori K, Kohda D, Ishino Y. Archaeal primase: bridging the gap between RNA and DNA polymerases. Curr Biol. 2001;11:452-6 pubmed
    ..Pfup41 is the first DNA polymerase that does not require primers for the synthesis of long DNA strands...
  9. Hashimoto K, Nakashima N, Ohara T, Maki S, Sugino A. The second subunit of DNA polymerase III (delta) is encoded by the HYS2 gene in Saccharomyces cerevisiae. Nucleic Acids Res. 1998;26:477-85 pubmed
    ..cerevisiae and is required for highly processive DNA synthesis catalyzed by DNA polymerase III in the presence of PCNA, RF-A and RF-C. ..

More Information


  1. Shimizu K, Kawasaki Y, Hiraga S, Tawaramoto M, Nakashima N, Sugino A. The fifth essential DNA polymerase phi in Saccharomyces cerevisiae is localized to the nucleolus and plays an important role in synthesis of rRNA. Proc Natl Acad Sci U S A. 2002;99:9133-8 pubmed
    ..Pol5p is localized exclusively to the nucleolus and binds near or at the enhancer region of rRNA-encoding DNA repeating units. ..
  2. Daimon K, Kawarabayasi Y, Kikuchi H, Sako Y, Ishino Y. Three proliferating cell nuclear antigen-like proteins found in the hyperthermophilic archaeon Aeropyrum pernix: interactions with the two DNA polymerases. J Bacteriol. 2002;184:687-94 pubmed
    ..Therefore, it is very interesting that three active PCNAs were found in one archaeal cell. Further analyses are necessary to determine whether each PCNA has specific roles, and moreover, how they reveal different functions in the cells...
  3. Yuasa M, Masutani C, Hirano A, Cohn M, Yamaizumi M, Nakatani Y, et al. A human DNA polymerase eta complex containing Rad18, Rad6 and Rev1; proteomic analysis and targeting of the complex to the chromatin-bound fraction of cells undergoing replication fork arrest. Genes Cells. 2006;11:731-44 pubmed
    ..These results suggest that arrested replication forks strengthen interactions among Poleta, Rad18/Rad6 and Rev1, consistent with the requirement for effective TLS by Poleta at sites of DNA lesions. ..
  4. Matsumura H, Takahashi H, Inoue T, Yamamoto T, Hashimoto H, Nishioka M, et al. Crystal structure of intein homing endonuclease II encoded in DNA polymerase gene from hyperthermophilic archaeon Thermococcus kodakaraensis strain KOD1. Proteins. 2006;63:711-5 pubmed publisher
  5. Kusumoto R, Masutani C, Shimmyo S, Iwai S, Hanaoka F. DNA binding properties of human DNA polymerase eta: implications for fidelity and polymerase switching of translesion synthesis. Genes Cells. 2004;9:1139-50 pubmed
    ..Our study provides mechanistic explanations for polymerase switching at TT dimer sites. ..
  6. Kusumoto R, Masutani C, Iwai S, Hanaoka F. Translesion synthesis by human DNA polymerase eta across thymine glycol lesions. Biochemistry. 2002;41:6090-9 pubmed
    ..The possible mechanisms of pol eta-catalyzed TLS are discussed on the basis of these results. ..
  7. Ohya T, Maki S, Kawasaki Y, Sugino A. Structure and function of the fourth subunit (Dpb4p) of DNA polymerase epsilon in Saccharomyces cerevisiae. Nucleic Acids Res. 2000;28:3846-52 pubmed
    ..cerevisiae, even if it is not essential for cell growth. Structural homologues of DPB4 are present in other eukaryotic genomes, suggesting that the complex structure of S. cerevisiae Polepsilon is conserved in eukaryotes. ..
  8. Cann I, Ishino S, Hayashi I, Komori K, Toh H, Morikawa K, et al. Functional interactions of a homolog of proliferating cell nuclear antigen with DNA polymerases in Archaea. J Bacteriol. 1999;181:6591-9 pubmed
    ..This is the first stage in elucidating the archaeal DNA replication mechanism...
  9. Nishioka M, Fujiwara S, Takagi M, Imanaka T. Characterization of two intein homing endonucleases encoded in the DNA polymerase gene of Pyrococcus kodakaraensis strain KOD1. Nucleic Acids Res. 1998;26:4409-12 pubmed
    ..kodakaraensis KOD1 was used as substrate. Therefore, it is suggested that these endonucleases are switching endonucleases whose function lies in the rearrangement of chromosomal DNA...
  10. Ogata N, Miura T. Elongation of tandem repetitive DNA by the DNA polymerase of the hyperthermophilic archaeon Thermococcus litoralis at a hairpin-coil transitional state: a model of amplification of a primordial simple DNA sequence. Biochemistry. 2000;39:13993-4001 pubmed
    ..We discuss the implication of this phenomenon, "hairpin elongation", from the standpoint of potential amplification of simple DNA sequences during the evolution of the genome...
  11. Ohkumo T, Kondo Y, Yokoi M, Tsukamoto T, Yamada A, Sugimoto T, et al. UV-B radiation induces epithelial tumors in mice lacking DNA polymerase eta and mesenchymal tumors in mice deficient for DNA polymerase iota. Mol Cell Biol. 2006;26:7696-706 pubmed
    ..These results suggest the involvement of the Pol eta and Pol iota proteins in UV-induced skin carcinogenesis. ..
  12. Masutani C, Kusumoto R, Yamada A, Dohmae N, Yokoi M, Yuasa M, et al. The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase eta. Nature. 1999;399:700-4 pubmed
    ..Together, these results indicate that DNA polymerase eta could be the XPV gene product. ..