Experts and Doctors on tobacco in Ikoma, Nara, Japan


Locale: Ikoma, Nara, Japan
Topic: tobacco

Top Publications

  1. Nagaya S, Yoshida K, Kato K, Akasaka K, Shinmyo A. An insulator element from the sea urchin Hemicentrotus pulcherrimus suppresses variation in transgene expression in cultured tobacco cells. Mol Genet Genomics. 2001;265:405-13 pubmed
    ..These results suggest that the insulator functions to suppress the variation normally associated with transgene expression in tobacco BY2 cells. ..
  2. Katoh A, Hashimoto T. Molecular biology of pyridine nucleotide and nicotine biosynthesis. Front Biosci. 2004;9:1577-86 pubmed
    ..Gene expression studies in tobacco suggest that part of the NAD pathway is coordinately regulated with nicotine biosynthesis. ..
  3. Sekine M, Ito M, Uemukai K, Maeda Y, Nakagami H, Shinmyo A. Isolation and characterization of the E2F-like gene in plants. FEBS Lett. 1999;460:117-22 pubmed
    ..This is the first report of the identification of a functionally equivalent E2F-like gene in plants...
  4. Yang S, Berberich T, Sano H, Kusano T. Specific association of transcripts of tbzF and tbz17, tobacco genes encoding basic region leucine zipper-type transcriptional activators, with guard cells of senescing leaves and/or flowers. Plant Physiol. 2001;127:23-32 pubmed
    ..These results suggest that TBZF and TBZ17 are both involved in controlling gene transcription related to functions of guard cells in senescing leaves and that TBZF bifunctionally acts in floral development...
  5. Yoda H, Ogawa M, Yamaguchi Y, Koizumi N, Kusano T, Sano H. Identification of early-responsive genes associated with the hypersensitive response to tobacco mosaic virus and characterization of a WRKY-type transcription factor in tobacco plants. Mol Genet Genomics. 2002;267:154-61 pubmed
    ..These results indicate the presence of salicylic acid-independent pathways for HR signal transduction, in which a novel type of WRKY protein(s) may play a critical role for the activation of defense. ..
  6. Shoji T, Winz R, Iwase T, Nakajima K, Yamada Y, Hashimoto T. Expression patterns of two tobacco isoflavone reductase-like genes and their possible roles in secondary metabolism in tobacco. Plant Mol Biol. 2002;50:427-40 pubmed
    ..The A622 expression patterns were qualitatively similar to those of putrescine N-methyltransferase, the first enzyme in nicotine biosynthesis, suggesting that A622 may function in the metabolism of nicotine or related alkaloids. ..
  7. Yang S, Berberich T, Miyazaki A, Sano H, Kusano T. Ntdin, a tobacco senescence-associated gene, is involved in molybdenum cofactor biosynthesis. Plant Cell Physiol. 2003;44:1037-44 pubmed
    ..Abscisic acid and indole-3-acidic acid, whose biosynthetic pathways require Moco, up-regulated Ntdin expression. Taken together, it is concluded that Ntdin functions in a certain step in Moco biosynthesis. ..
  8. Shoji T, Inai K, Yazaki Y, Sato Y, Takase H, Shitan N, et al. Multidrug and toxic compound extrusion-type transporters implicated in vacuolar sequestration of nicotine in tobacco roots. Plant Physiol. 2009;149:708-18 pubmed publisher
    ..The results imply that these MATE-type proteins transport tobacco alkaloids from the cytosol into the vacuole in exchange for protons in alkaloid-synthesizing root cells. ..
  9. Komaki S, Abe T, Coutuer S, Inz D, Russinova E, Hashimoto T. Nuclear-localized subtype of end-binding 1 protein regulates spindle organization in Arabidopsis. J Cell Sci. 2010;123:451-9 pubmed publisher
    ..These results suggest that a plant-specific EB1 subtype has evolved to function preferentially on the spindle microtubules by accumulating in the prophase nucleus...

More Information


  1. Shoji T, Kajikawa M, Hashimoto T. Clustered transcription factor genes regulate nicotine biosynthesis in tobacco. Plant Cell. 2010;22:3390-409 pubmed publisher
  2. Matsui T, Tabayashi A, Iwano M, Shinmyo A, Kato K, Nakayama H. Activity of the C-terminal-dependent vacuolar sorting signal of horseradish peroxidase C1a is enhanced by its secondary structure. Plant Cell Physiol. 2011;52:413-20 pubmed publisher
    ..We also found that the function of C6 is enhanced by the remaining N-terminal part of C15 which probably folds into an amphiphilic ?-helix. ..
  3. Kajikawa M, Shoji T, Kato A, Hashimoto T. Vacuole-localized berberine bridge enzyme-like proteins are required for a late step of nicotine biosynthesis in tobacco. Plant Physiol. 2011;155:2010-22 pubmed publisher
    ..These results indicate that BBLs are involved in a late oxidation step subsequent to the pyridine ring condensation reaction in the biosynthesis of tobacco alkaloids...
  4. Hara K, Yagi M, Koizumi N, Kusano T, Sano H. Screening of wound-responsive genes identifies an immediate-early expressed gene encoding a highly charged protein in mechanically wounded tobacco plants. Plant Cell Physiol. 2000;41:684-91 pubmed
    ..The KED polypeptide is composed of a highly hydrophilic N-terminal region and a relatively hydrophobic C-terminal region, suggesting that KED may function through electrostatic interactions with cellular components. ..