Experts and Doctors on recombinant proteins in Ikoma, Nara, Japan


Locale: Ikoma, Nara, Japan
Topic: recombinant proteins

Top Publications

  1. Yamamoto T, Matsui T, Nakafuku M, Iwamatsu A, Kaibuchi K. A novel GTPase-activating protein for R-Ras. J Biol Chem. 1995;270:30557-61 pubmed
    ..These results clearly indicate that p98 is a novel GAP for R-Ras. Thus, we designated this protein as R-Ras GAP. ..
  2. Matsui T, Amano M, Yamamoto T, Chihara K, Nakafuku M, Ito M, et al. Rho-associated kinase, a novel serine/threonine kinase, as a putative target for small GTP binding protein Rho. EMBO J. 1996;15:2208-16 pubmed
    ..Thus it is likely that Rho-kinase is a putative target serine/threonine kinase for Rho and serves as a mediator of the Rho-dependent signaling pathway. ..
  3. Kobayashi K, Kuroda S, Fukata M, Nakamura T, Nagase T, Nomura N, et al. p140Sra-1 (specifically Rac1-associated protein) is a novel specific target for Rac1 small GTPase. J Biol Chem. 1998;273:291-5 pubmed
    ..Sra-1 was cosedimented with filamentous actin (F-actin), indicating that Sra-1 directly interacts with F-actin. These results suggest that Sra-1 is a novel and specific target for Rac1. ..
  4. Fukata Y, Oshiro N, Kinoshita N, Kawano Y, Matsuoka Y, Bennett V, et al. Phosphorylation of adducin by Rho-kinase plays a crucial role in cell motility. J Cell Biol. 1999;145:347-61 pubmed
  5. Nakamura N, Oshiro N, Fukata Y, Amano M, Fukata M, Kuroda S, et al. Phosphorylation of ERM proteins at filopodia induced by Cdc42. Genes Cells. 2000;5:571-81 pubmed
    ..The formation of filopodia induced by Cdc42 is accompanied by phosphorylation of ERM proteins, and MRCK is a candidate for the kinase that phosphorylates ERM proteins at filopodia. ..
  6. Harigai M, Imamoto Y, Kamikubo H, Yamazaki Y, Kataoka M. Role of an N-terminal loop in the secondary structural change of photoactive yellow protein. Biochemistry. 2003;42:13893-900 pubmed
    ..The electrostatic interaction between this region and the beta-scaffold is likely to promote the conformational change of PYP(M) for recovery of PYP...
  7. Nishimura K, Ashida H, Ogawa T, Yokota A. A DEAD box protein is required for formation of a hidden break in Arabidopsis chloroplast 23S rRNA. Plant J. 2010;63:766-77 pubmed publisher
    ..We propose a molecular mechanism for the RH39-mediated fragmentation processing of 23S rRNA in chloroplasts. ..
  8. Nawata M, Tsutsumi H, Kobayashi Y, Unzai S, Mine S, Nakamura T, et al. Heat-induced native dimerization prevents amyloid formation by variable domain from immunoglobulin light-chain REI. FEBS J. 2017;284:3114-3127 pubmed publisher
    ..Structural data are available in the Protein Data Bank under the accession numbers 5XP1 and 5XQY. ..
  9. Shimizu T, Toumoto A, Ihara K, Shimizu M, Kyogoku Y, Ogawa N, et al. Crystal structure of PHO4 bHLH domain-DNA complex: flanking base recognition. EMBO J. 1997;16:4689-97 pubmed
    ..The residues involved in the E-box recognition are His5, Glu9 and Arg13, as already reported for bHLH/Zip proteins MAX and USF, and are different from those recognized by bHLH proteins MyoD and E47, although PHO4 is a bHLH protein. ..

More Information


  1. Nakajima K, Furutani I, Tachimoto H, Matsubara H, Hashimoto T. SPIRAL1 encodes a plant-specific microtubule-localized protein required for directional control of rapidly expanding Arabidopsis cells. Plant Cell. 2004;16:1178-90 pubmed
    ..These observations suggest that SPR1 is a plant-specific MT-localized protein required for the maintenance of growth anisotropy in rapidly elongating cells. ..
  2. Lieberherr D, Thao N, Nakashima A, Umemura K, Kawasaki T, Shimamoto K. A sphingolipid elicitor-inducible mitogen-activated protein kinase is regulated by the small GTPase OsRac1 and heterotrimeric G-protein in rice 1[w]. Plant Physiol. 2005;138:1644-52 pubmed publisher
    ..These results indicate that these two G-proteins regulate an elicitor-inducible MAPK in rice at the protein level...
  3. Shirai K, Yamazaki Y, Kamikubo H, Imamoto Y, Kataoka M. Attempt to simplify the amino-acid sequence of photoactive yellow protein with a set of simple rules. Proteins. 2007;67:821-33 pubmed
    ..The structural characterization of each chimeric protein indicates that the important information on the structure formation is encoded in the beta-scaffold region. ..
  4. Saito Y, Ashida H, Kojima C, Tamura H, Matsumura H, Kai Y, et al. Enzymatic characterization of 5-methylthioribose 1-phosphate isomerase from Bacillus subtilis. Biosci Biotechnol Biochem. 2007;71:2021-8 pubmed
    ..7 kJ mol(-1). The enzyme, with a molecular mass of 76 kDa, was composed of two subunits. The equilibrium constant in the reversible isomerase reaction [MTRu-1-P]/[MTR-1-P] was 6. We discuss the possible reaction mechanism...
  5. Ohki I, Amida H, Yamada R, Sugihara M, Ishigaki T, Tate S. Surface plasmon resonance study on functional significance of clustered organization of lectin-like oxidized LDL receptor (LOX-1). Biochim Biophys Acta. 2011;1814:345-54 pubmed publisher
    ..In combination with the analyses on the loss-of-binding mutant W150A, we concluded that the clustered organization of the properly formed homodimeric CTLD is essential for the strong binding of LOX-1 to OxLDL. ..