Experts and Doctors on freezing in Sapporo, Hokkaido, Japan


Locale: Sapporo, Hokkaido, Japan
Topic: freezing

Top Publications

  1. Uchida T, Takeya S. Powder X-ray diffraction observations of ice crystals formed from disaccharide solutions. Phys Chem Chem Phys. 2010;12:15034-9 pubmed publisher
  2. Katayama T, Tanaka M, Moriizumi J, Nakamura T, Brouchkov A, Douglas T, et al. Phylogenetic analysis of bacteria preserved in a permafrost ice wedge for 25,000 years. Appl Environ Microbiol. 2007;73:2360-3 pubmed
    ..The radiocarbon age of the ice wedge was determined. Our results suggest that the bacteria in the ice wedge adapted to the frozen conditions have survived for 25,000 years. ..
  3. Miura K, Ohgiya S, Hoshino T, Nemoto N, Suetake T, Miura A, et al. NMR analysis of type III antifreeze protein intramolecular dimer. Structural basis for enhanced activity. J Biol Chem. 2001;276:1304-10 pubmed
    ..This manner of ice binding caused by the unique structural topology of RD3 is thought to be crucial for the significant enhancement of antifreeze activity, especially at low AFP concentrations. ..
  4. Suzuki K, Asano A, Eriksson B, Niwa K, Nagai T, Rodriguez Martinez H. Capacitation status and in vitro fertility of boar spermatozoa: effects of seminal plasma, cumulus-oocyte-complexes-conditioned medium and hyaluronan. Int J Androl. 2002;25:84-93 pubmed
    ..The unclear effects of frozen-thawed CCM and a low dose of HA on penetration rates of boar spermatozoa call for further researches of their function in vivo. ..
  5. Matsumoto S, Matsusita M, Morita T, Kamachi H, Tsukiyama S, Furukawa Y, et al. Effects of synthetic antifreeze glycoprotein analogue on islet cell survival and function during cryopreservation. Cryobiology. 2006;52:90-8 pubmed
    ..In conclusion, the attenuation of ice crystallization by syAFGP improves islet survival and function following cryopreservation and thawing. ..
  6. Tohyama H, Yasuda K. The effects of stress enhancement on the extracellular matrix and fibroblasts in the patellar tendon. J Biomech. 2000;33:559-65 pubmed
  7. Kasuga J, Takata N, Yamane K, Kuroda K, Arakawa K, Fujikawa S. Larch (Larix kaempferi) xylem parenchyma cells respond to subfreezing temperature by deep supercooling. Cryo Letters. 2007;28:77-81 pubmed
    ..The results showed that XPCs in larch adapts to subfreezing temperature by deep supercooling throughout all seasons. Such freezing behavior is the same as that of XPCs in boreal hardwood species. ..
  8. Utsumi Y, Sano Y, Funada R, Ohtani J, Fujikawa S. Seasonal and perennial changes in the distribution of water in the sapwood of conifers in a sub-frigid zone. Plant Physiol. 2003;131:1826-33 pubmed
    ..The region involved in water transport in conifers did not include the entire sapwood and differed among annual rings. ..
  9. Ju Y, Tohyama H, Kondo E, Yoshikawa T, Muneta T, Shinomiya K, et al. Effects of local administration of vascular endothelial growth factor on properties of the in situ frozen-thawed anterior cruciate ligament in rabbits. Am J Sports Med. 2006;34:84-91 pubmed
    ..An application of the recombinant anterior cruciate ligament is a potential future strategy to enhance revascularization of the autograft in anterior cruciate ligament reconstruction. ..

More Information


  1. Koike M, Takezawa D, Arakawa K, Yoshida S. Accumulation of 19-kDa plasma membrane polypeptide during induction of freezing tolerance in wheat suspension-cultured cells by abscisic acid. Plant Cell Physiol. 1997;38:707-16 pubmed
    ..2). Expression of WPM-1 mRNA was dramatically induced by 50 microM ABA within a few hours. These results suggest that the AWPM-19 might be closely associated with the ABA-induced increase in freezing tolerance in wheat cultured cells...
  2. Minami A, Nagao M, Arakawa K, Fujikawa S, Takezawa D. Abscisic acid-induced freezing tolerance in the moss Physcomitrella patens is accompanied by increased expression of stress-related genes. J Plant Physiol. 2003;160:475-83 pubmed publisher
    ..These results suggest that ABA and environmental stresses positively affect the expression of common genes that participate in protection of protonema cells leading to the development of freezing tolerance...
  3. Yamada T, Kuroda K, Jitsuyama Y, Takezawa D, Arakawa K, Fujikawa S. Roles of the plasma membrane and the cell wall in the responses of plant cells to freezing. Planta. 2002;215:770-8 pubmed
    ..It appears, therefore, that not only the plasma membrane but also the cell wall greatly influences the freezing behavior of plant cells...
  4. Jitsuyama Y, Suzuki T, Harada T, Fujikawa S. Sucrose incubation increases freezing tolerance of asparagus (Asparagus officinalis L.) embryogenic cell suspensions. Cryo Letters. 2002;23:103-12 pubmed
    ..These results suggest that freezing tolerance of asparagus embryogenic cells is increased by a complex mechanism involving notably changes in cell ultrastructure and accumulation of certain sugars and proteins during sugar incubation...
  5. Shimono K, Furutani Y, Kandori H, Kamo N. A pharaonis phoborhodopsin mutant with the same retinal binding site residues as in bacteriorhodopsin. Biochemistry. 2002;41:6504-9 pubmed
    ..We conclude that the principal factor of the smaller than expected opsin shift in BR/ppR is the strong association of the Schiff base with the surrounding counterion complex. ..
  6. Sugimoto M, Hyodoh H, Rokukawa M, Kanazawa A, Murakami R, Shimizu J, et al. Freezing effect on brain density in postmortem CT. Leg Med (Tokyo). 2016;18:62-5 pubmed publisher
    ..When lower CT density presents in postmortem CT, the freezing effect may need to be considered and the physician should evaluate the cadaver's postmortem condition to prevent misdiagnoses. ..
  7. Iwamoto M, Furutani Y, Kamo N, Kandori H. Proton transfer reactions in the F86D and F86E mutants of pharaonis phoborhodopsin (sensory rhodopsin II). Biochemistry. 2003;42:2790-6 pubmed
    ..Therefore, it is likely that a proton can be conducted in either direction, the Schiff base or the bulk, in the open M-like structure of F86D and F86E...
  8. Kim M, Sasaki K, Imai R. Cold shock domain protein 3 regulates freezing tolerance in Arabidopsis thaliana. J Biol Chem. 2009;284:23454-60 pubmed publisher
    ..Overexpression of AtCSP3 in transgenic plants conferred enhanced freezing tolerance over wild-type plants. Together, the data demonstrated an essential role of RNA chaperones for cold adaptation in higher plants. ..