Experts and Doctors on thermus thermophilus in Hyōgo, Japan


Locale: Hyōgo, Japan
Topic: thermus thermophilus

Top Publications

  1. Ebihara A, Yao M, Masui R, Tanaka I, Yokoyama S, Kuramitsu S. Crystal structure of hypothetical protein TTHB192 from Thermus thermophilus HB8 reveals a new protein family with an RNA recognition motif-like domain. Protein Sci. 2006;15:1494-9 pubmed publisher
    ..This report shows that TTHB192 and its sequence homologs adopt an RNA recognition motif-like domain and provides the first testable functional hypothesis for this protein family...
  2. Staals R, Agari Y, Maki Yonekura S, Zhu Y, Taylor D, van Duijn E, et al. Structure and activity of the RNA-targeting Type III-B CRISPR-Cas complex of Thermus thermophilus. Mol Cell. 2013;52:135-145 pubmed publisher
    ..Despite having a backbone of only four Cmr4 subunits and being both longer and narrower, the overall architecture of TtCmr resembles that of Type I Cascade complexes. ..
  3. Kobayashi S, Masui R, Yokoyama S, Kuramitsu S, Takagi H. A novel metal-activated L-serine O-acetyltransferase from Thermus thermophilus HB8. J Biochem. 2004;136:629-34 pubmed
    ..These results indicate that the T. thermophilus SAT is a novel type of enzyme different from other members of this protein family. ..
  4. Bagautdinov B, Kunishima N. Crystal structures of shikimate dehydrogenase AroE from Thermus thermophilus HB8 and its cofactor and substrate complexes: insights into the enzymatic mechanism. J Mol Biol. 2007;373:424-38 pubmed publisher
    ..The ternary complex TtAroE:NADP(H):shikimate allows unambiguous visualization of the SDH permitting elucidation of the roles of conserved residues Lys64 and Asp100 in the hydride ion transfer between NADP(H) and shikimate...
  5. Lokanath N, Ohshima N, Takio K, Shiromizu I, Kuroishi C, Okazaki N, et al. Crystal structure of novel NADP-dependent 3-hydroxyisobutyrate dehydrogenase from Thermus thermophilus HB8. J Mol Biol. 2005;352:905-17 pubmed publisher
    ..A kinetic study confirms that this enzyme has strict substrate specificity for 3-hydroxyisobutyrate and serine, but it cannot distinguish the chirality of the substrates. Lys165 is likely the catalytic residue of the enzyme...
  6. Agari Y, Sakamoto K, Tamakoshi M, Oshima T, Kuramitsu S, Shinkai A. Transcription profile of Thermus thermophilus CRISPR systems after phage infection. J Mol Biol. 2010;395:270-81 pubmed publisher
    ..These findings will facilitate understanding of the host response mechanism following phage infection. ..
  7. Agari Y, Sakamoto K, Yutani K, Kuramitsu S, Shinkai A. Structure and function of a TetR family transcriptional regulator, SbtR, from thermus thermophilus HB8. Proteins. 2013;81:1166-78 pubmed publisher
    ..The formation and reduction of the disulfide bond might function in controlling the ligand-binding affinity of this transcriptional regulator...
  8. Rehse P, Kitao T, Tahirov T. Structure of a closed-form uroporphyrinogen-III C-methyltransferase from Thermus thermophilus. Acta Crystallogr D Biol Crystallogr. 2005;61:913-9 pubmed publisher
    ..The T. thermophilus structure has this site restricted by the interaction of a flexible loop structure with a highly conserved residue, suggesting a mechanistic role. This structure represents the ;closed' form of the protein...
  9. Rehse P, Kuroishi C, Tahirov T. Structure of the RNA-processing inhibitor RraA from Thermus thermophilis. Acta Crystallogr D Biol Crystallogr. 2004;60:1997-2002 pubmed
    ..The fold does not support the postulated methylation function. Genomic analysis, specifically a lack of an RNase E homologue in cases where homologues to RraA exist, indicates that the function is still obscure. ..

More Information


  1. Nakai T, Nakagawa N, Maoka N, Masui R, Kuramitsu S, Kamiya N. Ligand-induced conformational changes and a reaction intermediate in branched-chain 2-oxo acid dehydrogenase (E1) from Thermus thermophilus HB8, as revealed by X-ray crystallography. J Mol Biol. 2004;337:1011-33 pubmed publisher
    ..MOPA and of E1(ceim).lipoamide built from the above structures suggest that His273alpha and His129beta' are potential proton donors to the carbonyl group of a BCOA and to the proximal sulfur atom on the lipoamide, respectively...
  2. Tanaka T, Niwa H, Yutani K, Kuramitsu S, Yokoyama S, Kumarevel T. Crystal structure of TTHA0061, an uncharacterized protein from Thermus thermophilus HB8, reveals a novel fold. Biochem Biophys Res Commun. 2010;400:258-64 pubmed publisher
    ..Differential scanning calorimetry analysis suggested that the thermostability of TTHA0061 increased at pH ranges of 5.8-6.2, perhaps due to the abundance of glutamic acid residues. ..
  3. Rehse P, Ohshima N, Nodake Y, Tahirov T. Crystallographic structure and biochemical analysis of the Thermus thermophilus osmotically inducible protein C. J Mol Biol. 2004;338:959-68 pubmed publisher
    ..The active site consists of two cysteine residues from one monomer and an arginine and glutamic acid from the other. Enzymatic assays have revealed that T.thermophilus OsmC has a hydroperoxide peroxidase activity...
  4. Shinkai A, Ohbayashi N, Terada T, Shirouzu M, Kuramitsu S, Yokoyama S. Identification of promoters recognized by RNA polymerase-sigmaE holoenzyme from Thermus thermophilus HB8. J Bacteriol. 2007;189:8758-64 pubmed
    ..With the combination of in vitro transcription assay and GeneChip technology, we identified three promoters recognized by sigma(E). The predicted consensus promoter sequence for sigma(E) is 5'-CA(A/T)(A/C)C(A/C)-N(15)-CCGTA-3'. ..
  5. Sugahara M, Asada Y, Morikawa Y, Kageyama Y, Kunishima N. Nucleant-mediated protein crystallization with the application of microporous synthetic zeolites. Acta Crystallogr D Biol Crystallogr. 2008;64:686-95 pubmed publisher
    ..The hetero-epitaxic growth of the zeolite-mediated crystals was confirmed by a crystal-packing analysis which revealed a layer-like structure in the crystal lattice...
  6. Hayashida M, Kim S, Takeda K, Hisano T, Miki K. Crystal structure of N-acylamino acid racemase from Thermus thermophilus HB8. Proteins. 2008;71:519-23 pubmed publisher
  7. Inagaki E, Ohshima N, Takahashi H, Kuroishi C, Yokoyama S, Tahirov T. Crystal structure of Thermus thermophilus Delta1-pyrroline-5-carboxylate dehydrogenase. J Mol Biol. 2006;362:490-501 pubmed publisher
    ..8 A, 1.9 A, and 1.4 A resolution, respectively. The solved structures suggest an overall view of the P5CDh catalytic mechanism and provide insights into the P5CDh deficiencies in the case of the human type II hyperprolinemia...
  8. Asada Y, Sawano M, Ogasahara K, Nakamura J, Ota M, Kuroishi C, et al. Stabilization mechanism of the tryptophan synthase alpha-subunit from Thermus thermophilus HB8: X-ray crystallographic analysis and calorimetry. J Biochem. 2005;138:343-53 pubmed publisher
    ..The results of calorimetry suggest that the residual structure of the Tt-alpha-subunit in the denatured state contributes to the stabilization...
  9. Fukui K, Takahata Y, Nakagawa N, Kuramitsu S, Masui R. Analysis of a nuclease activity of catalytic domain of Thermus thermophilus MutS2 by high-accuracy mass spectrometry. Nucleic Acids Res. 2007;35:e100 pubmed
    ..The simultaneous identification of the innumerable fragments was achieved by the extremely high-accuracy of ESI-FT ICR MS. ..
  10. Kim S, Miyatake H, Hisano T, Iwasaki W, Ebihara A, Miki K. Crystallization and preliminary X-ray analysis of the oxygenase component (HpaB) of 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007;63:556-9 pubmed
    ..50 A. MAD data were successfully collected for structural determination using these crystals. ..
  11. Tanaka T, Sawano M, Ogasahara K, Sakaguchi Y, Bagautdinov B, Katoh E, et al. Hyper-thermostability of CutA1 protein, with a denaturation temperature of nearly 150 degrees C. FEBS Lett. 2006;580:4224-30 pubmed
  12. Yamamoto H, Miwa H, Kunishima N. Crystal structure of glucose-6-phosphate isomerase from Thermus thermophilus HB8 showing a snapshot of active dimeric state. J Mol Biol. 2008;382:747-62 pubmed publisher
    ..From these results, it is suggested that transient dimer formation is sufficient for the catalytic function and that the TtGPI protomer itself has intrinsically higher thermal stability. ..
  13. Sugahara M, Nodake Y, Sugahara M, Kunishima N. Crystal structure of dehydroquinate synthase from Thermus thermophilus HB8 showing functional importance of the dimeric state. Proteins. 2005;58:249-52 pubmed publisher
  14. Kichise T, Hisano T, Takeda K, Miki K. Crystal structure of phenylacetic acid degradation protein PaaG from Thermus thermophilus HB8. Proteins. 2009;76:779-86 pubmed publisher
  15. Hisanaga Y, Ago H, Nakagawa N, Hamada K, Ida K, Yamamoto M, et al. Structural basis of the substrate-specific two-step catalysis of long chain fatty acyl-CoA synthetase dimer. J Biol Chem. 2004;279:31717-26 pubmed publisher
    ..Based on these high resolution crystal structures, we propose a unidirectional Bi Uni Uni Bi Ping-Pong mechanism for the two-step acylation by ttLC-FACS...
  16. Nishio K, Nodake Y, Hamada K, Suto K, Nakagawa N, Kuramitsu S, et al. Expression, purification, crystallization and preliminary X-ray studies of geranylgeranyl diphosphate synthase from Thermus thermophilus HB8. Acta Crystallogr D Biol Crystallogr. 2004;60:178-80 pubmed
    ..88, c = 73.37 A. There were two homodimers in the asymmetric unit. A native data set was collected to 1.55 A resolution and a data set suitable for MAD phasing was collected to 2.40 A resolution on beamline BL40B2 at SPring-8. ..
  17. Iino H, Shimizu N, Goto M, Ebihara A, Fukui K, Hirotsu K, et al. Crystal structure of the tandem-type universal stress protein TTHA0350 from Thermus thermophilus HB8. J Biochem. 2011;150:295-302 pubmed publisher
    ..The loop interacting with ATP in the C-terminal domain is in a conformation quite different from that in the N-terminal domain...
  18. Vassylyev D, Sekine S, Laptenko O, Lee J, Vassylyeva M, Borukhov S, et al. Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 A resolution. Nature. 2002;417:712-9 pubmed publisher
    ..The holoenzyme structure provides insight into the structural organization of transcription intermediate complexes and into the mechanism of transcription initiation...
  19. Kawaguchi S, Muller J, Linde D, Kuramitsu S, Shibata T, Inoue Y, et al. The crystal structure of the ttCsaA protein: an export-related chaperone from Thermus thermophilus. EMBO J. 2001;20:562-9 pubmed
    ..These results raise the intriguing possibility that CsaA proteins possess a second, tRNA-binding activity in addition to their export-related function. ..
  20. Agari Y, Kuramitsu S, Shinkai A. Identification of novel genes regulated by the oxidative stress-responsive transcriptional activator SdrP in Thermus thermophilus HB8. FEMS Microbiol Lett. 2010;313:127-34 pubmed publisher
    ..These results indicate that the main function of SdrP is in the oxidative stress response. ..
  21. Agari Y, Agari K, Sakamoto K, Kuramitsu S, Shinkai A. TetR-family transcriptional repressor Thermus thermophilus FadR controls fatty acid degradation. Microbiology. 2011;157:1589-601 pubmed publisher
    ..thermophilus HB8, medium-to-long straight-chain fatty acids can be used for metabolic energy under the control of FadR, although the major fatty acids found in this strain are iso- and anteiso-branched-chain (C15 and 17) fatty acids...
  22. Agari Y, Sakamoto K, Kuramitsu S, Shinkai A. Transcriptional repression mediated by a TetR family protein, PfmR, from Thermus thermophilus HB8. J Bacteriol. 2012;194:4630-41 pubmed publisher
    ..The center of the PfmR molecule contains a tunnel-like pocket, which may be the ligand-binding site of this regulator...
  23. Sekine S, Nureki O, Shimada A, Vassylyev D, Yokoyama S. Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase. Nat Struct Biol. 2001;8:203-6 pubmed publisher
    ..The mutation of Arg 358 to Gln resulted in a GluRS that does not discriminate between the Glu and Gln anticodons. This change mimics the reverse course of GluRS evolution from anticodon 'non-dicsriminating' to 'discriminating'...
  24. Nakai T, Nakagawa N, Maoka N, Masui R, Kuramitsu S, Kamiya N. Structure of P-protein of the glycine cleavage system: implications for nonketotic hyperglycinemia. EMBO J. 2005;24:1523-36 pubmed publisher
    ..These results provide insights into the molecular basis of nonketotic hyperglycinemia...
  25. Lokanath N, Shiromizu I, Ohshima N, Nodake Y, Sugahara M, Yokoyama S, et al. Structure of aldolase from Thermus thermophilus HB8 showing the contribution of oligomeric state to thermostability. Acta Crystallogr D Biol Crystallogr. 2004;60:1816-23 pubmed publisher
    ..From these results, it is concluded that the oligomeric state of 2-deoxyribose-5-phosphate aldolase is important for the thermostability and not for the catalytic function...
  26. Nakai T, Ishijima J, Masui R, Kuramitsu S, Kamiya N. Structure of Thermus thermophilus HB8 H-protein of the glycine-cleavage system, resolved by a six-dimensional molecular-replacement method. Acta Crystallogr D Biol Crystallogr. 2003;59:1610-8 pubmed
    ..Meanwhile, the lipoyl-lysine arm of the T. thermophilus H-protein was found to be more flexible than that of the pea H-protein, supporting the hypothesis that H-protein does not form a stable complex with L-protein during the reaction. ..
  27. Hoseki J, Okamoto A, Masui R, Shibata T, Inoue Y, Yokoyama S, et al. Crystal structure of a family 4 uracil-DNA glycosylase from Thermus thermophilus HB8. J Mol Biol. 2003;333:515-26 pubmed
    ..coli MUG (family 2). These results suggest that the mechanism by which family 4 UDGs remove uracils from DNA is similar to that of family 1 enzymes...
  28. Agari Y, Kashihara A, Yokoyama S, Kuramitsu S, Shinkai A. Global gene expression mediated by Thermus thermophilus SdrP, a CRP/FNR family transcriptional regulator. Mol Microbiol. 2008;70:60-75 pubmed publisher
    ..coli CRP. Based on the properties of the SdrP-regulated genes found in this study, it is speculated that SdrP is involved in nutrient and energy supply, redox control, and polyadenylation of mRNA. ..
  29. Kitamura Y, Ebihara A, Agari Y, Shinkai A, Hirotsu K, Kuramitsu S. Structure of D-alanine-D-alanine ligase from Thermus thermophilus HB8: cumulative conformational change and enzyme-ligand interactions. Acta Crystallogr D Biol Crystallogr. 2009;65:1098-106 pubmed publisher
    ..The catalytic process accompanied by the cumulative conformational change has been elucidated based on the intermediate models in order to provide new insights regarding the details of the catalytic mechanism. ..
  30. Fukui K, Nishida M, Nakagawa N, Masui R, Kuramitsu S. Bound nucleotide controls the endonuclease activity of mismatch repair enzyme MutL. J Biol Chem. 2008;283:12136-45 pubmed publisher
    ..Complementation experiments revealed that the endonuclease activity of ttMutL and its regulation by ATP binding are necessary for DNA repair in vivo. ..
  31. Tahirov T, Inagaki E, Ohshima N, Kitao T, Kuroishi C, Ukita Y, et al. Crystal structure of purine nucleoside phosphorylase from Thermus thermophilus. J Mol Biol. 2004;337:1149-60 pubmed publisher
    ..The obtained experimental data suggest that the catalytic properties of the T.thermophilus enzyme are reminiscent of the trimeric rather than hexameric purine nucleoside phosphorylases...
  32. Takahashi H, Inagaki E, Fujimoto Y, Kuroishi C, Nodake Y, Nakamura Y, et al. Structure and implications for the thermal stability of phosphopantetheine adenylyltransferase from Thermus thermophilus. Acta Crystallogr D Biol Crystallogr. 2004;60:97-104 pubmed
    ..coli PPAT. Comparative analysis also revealed that the higher stability of Tt PPAT arises from stabilization of each subunit by hydrophobic effects, hydrogen bonds and entropic effects...
  33. Fukui K, Wakamatsu T, Agari Y, Masui R, Kuramitsu S. Inactivation of the DNA repair genes mutS, mutL or the anti-recombination gene mutS2 leads to activation of vitamin B1 biosynthesis genes. PLoS ONE. 2011;6:e19053 pubmed publisher
    ..These results suggested that bacterial cells sense the accumulation of oxidative DNA damage or absence of DNA repair activity, and signal the information to the transcriptional regulation machinery for an ROS-detoxifying system...
  34. Kim S, Hisano T, Takeda K, Iwasaki W, Ebihara A, Miki K. Crystal structure of the oxygenase component (HpaB) of the 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8. J Biol Chem. 2007;282:33107-17 pubmed publisher
    ..Arg-100 is located adjacent to the putative oxygen-binding site and may be involved in the formation and stabilization of the C4a-hydroperoxyflavin intermediate...
  35. Kim S, Hisano T, Iwasaki W, Ebihara A, Miki K. Crystal structure of the flavin reductase component (HpaC) of 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8: Structural basis for the flavin affinity. Proteins. 2008;70:718-30 pubmed
  36. Sekine S, Nureki O, Dubois D, Bernier S, Ch nevert R, Lapointe J, et al. ATP binding by glutamyl-tRNA synthetase is switched to the productive mode by tRNA binding. EMBO J. 2003;22:676-88 pubmed publisher
    ..Therefore, tRNA binding to GluRS switches the ATP-binding mode. The interactions of the three tRNA(Glu) regions with GluRS cause conformational changes around the ATP-binding site, and allow ATP to bind to the 'productive' subsite...
  37. Iwasaki W, Miki K. Crystal structure of the stationary phase survival protein SurE with metal ion and AMP. J Mol Biol. 2007;371:123-36 pubmed publisher
    ..The structural features and the absence of negative cooperativity imply the half-of-the-sites reactivity mechanism resulting from a pre-existing tendency toward structural asymmetry...
  38. Shinkai A, Kira S, Nakagawa N, Kashihara A, Kuramitsu S, Yokoyama S. Transcription activation mediated by a cyclic AMP receptor protein from Thermus thermophilus HB8. J Bacteriol. 2007;189:3891-901 pubmed
    ..thermophilus-specific proteins of unknown function. These results suggest a role for cAMP signal transduction in T. thermophilus and imply the T. thermophilus CRP is a cAMP-responsive regulator. ..
  39. Hamada K, Ago H, Sugahara M, Nodake Y, Kuramitsu S, Miyano M. Oxyanion hole-stabilized stereospecific isomerization in ribose-5-phosphate isomerase (Rpi). J Biol Chem. 2003;278:49183-90 pubmed publisher
    ..R5P bound as the ring-opened reaction intermediate clarified the high stereoselectivity of the catalysis and is consistent with an aldose-ketose conversion by Rpi that proceeds via a cis-enediolate intermediate...
  40. Sakamoto K, Agari Y, Yokoyama S, Kuramitsu S, Shinkai A. Functional identification of an anti-sigmaE factor from Thermus thermophilus HB8. Gene. 2008;423:153-9 pubmed publisher
    ..Thus, TTHB212 protein was identified as an anti-sigma(E) factor. These findings indicate that T. thermophilus HB8 has a regulatory system involving sigma(E) and anti-sigma(E) factors. ..
  41. Takahashi H, Inagaki E, Kuroishi C, Tahirov T. Structure of the Thermus thermophilus putative periplasmic glutamate/glutamine-binding protein. Acta Crystallogr D Biol Crystallogr. 2004;60:1846-54 pubmed
    ..thermophilus HB8 molecule is most likely to be an L-glutamate and/or an L-glutamine-binding protein related to the cluster 3 periplasmic receptors. However, the geometry of ligand binding is unique to the T. thermophilus HB8 molecule. ..
  42. Kunishima N, Asada Y, Sugahara M, Ishijima J, Nodake Y, Sugahara M, et al. A novel induced-fit reaction mechanism of asymmetric hot dog thioesterase PAAI. J Mol Biol. 2005;352:212-28 pubmed publisher
  43. Sawano M, Yamamoto H, Ogasahara K, Kidokoro S, Katoh S, Ohnuma T, et al. Thermodynamic basis for the stabilities of three CutA1s from Pyrococcus horikoshii,Thermus thermophilus, and Oryza sativa, with unusually high denaturation temperatures. Biochemistry. 2008;47:721-30 pubmed
  44. Omi R, Goto M, Miyahara I, Manzoku M, Ebihara A, Hirotsu K. Crystal structure of monofunctional histidinol phosphate phosphatase from Thermus thermophilus HB8. Biochemistry. 2007;46:12618-27 pubmed
  45. Sugahara M, Ohshima N, Ukita Y, Sugahara M, Kunishima N. Structure of ATP-dependent phosphoenolpyruvate carboxykinase from Thermus thermophilus HB8 showing the structural basis of induced fit and thermostability. Acta Crystallogr D Biol Crystallogr. 2005;61:1500-7 pubmed
    ..A bound calcium observed in the N-terminal domain of TtPEPCK probably contributes to the thermal stability. A combination of hydrophobic effects, ion pairs and entropic effects might also contribute to the thermostability of TtPEPCK. ..
  46. Nakabayashi M, Shibata N, Komori H, Ueda Y, Iino H, Ebihara A, et al. Structure of a conserved hypothetical protein, TTHA0849 from Thermus thermophilus HB8, at 2.4 A resolution: a putative member of the StAR-related lipid-transfer (START) domain superfamily. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005;61:1027-31 pubmed
    ..However, the size of the ligand-binding cavity is distinctly smaller than other START domain-containing proteins, suggesting that it catalyses the transfer of smaller ligand molecules. ..
  47. Nakano N, Okazaki N, Satoh S, Takio K, Kuramitsu S, Shinkai A, et al. Structure of the stand-alone RAM-domain protein from Thermus thermophilus HB8. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006;62:855-60 pubmed
    ..This decameric structure is unique compared with the octameric and dodecameric structures found for other stand-alone RAM-domain proteins and the C-terminal RAM domains of Lrp/AsnC-family proteins. ..
  48. Taka J, Ogasahara K, Jeyakanthan J, Kunishima N, Kuroishi C, Sugahara M, et al. Stabilization due to dimer formation of phosphoribosyl anthranilate isomerase from Thermus thermophilus HB8: X-ray Analysis and DSC experiments. J Biochem. 2005;137:569-78 pubmed
  49. Kanagawa M, Baba S, Ebihara A, Shinkai A, Hirotsu K, Mega R, et al. Structures of hypoxanthine-guanine phosphoribosyltransferase (TTHA0220) from Thermus thermophilus HB8. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010;66:893-8 pubmed publisher
    ..The group II enzymes are characterized by an N-terminal extension with additional secondary elements and a long loop connecting the second alpha-helix and beta-strand compared with the group I enzymes. ..
  50. Inagaki E, Ukita Y, Kumei M, Kajihara Y, Tahirov T. Crystallization and preliminary crystallographic analysis of 2-keto-3-deoxygluconate kinase from Thermus thermophilus. Acta Crystallogr D Biol Crystallogr. 2004;60:761-3 pubmed
    ..72, c = 321.61 A and with bound KDG plus the ATP analogue AMP-PNP (TtKDGK-ATP-KDG), with unit-cell parameters a = b = 84.32, c = 168.7 A, were also prepared and characterized. ..
  51. Iwasaki W, Miyatake H, Miki K. Crystal structure of the small form of glucose-inhibited division protein A from Thermus thermophilus HB8. Proteins. 2005;61:1121-6 pubmed
  52. Jeyakanthan J, Taka J, Kikuchi A, Kuroishi C, Yutani K, Shiro Y. Purification, crystallization and preliminary X-ray crystallographic study of the L-fuculose-1-phosphate aldolase (FucA) from Thermus thermophilus HB8. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005;61:1075-7 pubmed
    ..2%(v/v). Three-wavelength diffraction MAD data were collected to 2.3 A from zinc-containing crystals. Native diffraction data to 1.9 A resolution have been collected using synchrotron radiation at SPring-8. ..
  53. Kumar S, Pampa K, Manjula M, Abdoh M, Kunishima N, Lokanath N. Crystal structure studies of NADP+ dependent isocitrate dehydrogenase from Thermus thermophilus exhibiting a novel terminal domain. Biochem Biophys Res Commun. 2014;449:107-13 pubmed publisher
    ..Overall, the TtIDH structure with novel terminal domain may be categorized as a first structure of subfamily of type IV. ..