Experts and Doctors on saccharomyces cerevisiae proteins in Tokyo, Japan

Summary

Locale: Tokyo, Japan
Topic: saccharomyces cerevisiae proteins

Top Publications

  1. Sakai A, Chibazakura T, Shimizu Y, Hishinuma F. Molecular analysis of POP2 gene, a gene required for glucose-derepression of gene expression in Saccharomyces cerevisiae. Nucleic Acids Res. 1992;20:6227-33 pubmed
  2. Qadota H, Python C, Inoue S, Arisawa M, Anraku Y, Zheng Y, et al. Identification of yeast Rho1p GTPase as a regulatory subunit of 1,3-beta-glucan synthase. Science. 1996;272:279-81 pubmed
    ..Both proteins were localized predominantly at sites of cell wall remodeling. Therefore, it appears that Rho1p is a regulatory subunit of beta(1-->3)glucan synthase. ..
  3. Tanaka H, Tanaka K, Murakami H, Okayama H. Fission yeast cdc24 is a replication factor C- and proliferating cell nuclear antigen-interacting factor essential for S-phase completion. Mol Cell Biol. 1999;19:1038-48 pubmed
    ..These results indicate that Cdc24 is an RFC- and PCNA-interacting factor required for DNA replication and might serve as a target for regulation. ..
  4. Zhang L, Onda K, Imai R, Fukuda R, Horiuchi H, Ohta A. Growth temperature downshift induces antioxidant response in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2003;307:308-14 pubmed
    ..However, deletion of YAP1 did not reduce the elevated mRNA levels of the antioxidant genes. This suggests that the temperature downshift-induced increase in the mRNA level of anti-oxidant genes is YAP1-independent. ..
  5. Sakasegawa Y, Hachiya N, Tsukita S, Kaneko K. Ecm10p localizes in yeast mitochondrial nucleoids and its overexpression induces extensive mitochondrial DNA aggregations. Biochem Biophys Res Commun. 2003;309:217-21 pubmed
    ..Overexpression of ecm10p induces extensive mitochondrial DNA aggregations, which might be due to aberrant mitochondrial DNA cleavages through an altered endonuclease activity in mitochondrial nucleoids. ..
  6. Yamazaki Sato H, Tanida I, Ueno T, Kominami E. The carboxyl terminal 17 amino acids within Apg7 are essential for Apg8 lipidation, but not for Apg12 conjugation. FEBS Lett. 2003;551:71-7 pubmed
    ..These results suggest that the carboxyl terminal 17 amino acids of Apg7 play a specific role in Apg8 lipidation indispensable for the Cvt pathway and autophagy. ..
  7. Hara H, Nakayashiki T, Crist C, Nakamura Y. Prion domain interaction responsible for species discrimination in yeast [PSI+] transmission. Genes Cells. 2003;8:925-39 pubmed
    ..NR as well as NQ alone coalesces into existing [PSI+] aggregates, showing their independent potentials to interact with the identical sequence in the [PSI+] conformer. The role of NQ and NR in [PSI+] prion formation is discussed. ..
  8. Iwamoto K, Kobayashi S, Fukuda R, Umeda M, Kobayashi T, Ohta A. Local exposure of phosphatidylethanolamine on the yeast plasma membrane is implicated in cell polarity. Genes Cells. 2004;9:891-903 pubmed
  9. Moriya H, Shimizu Yoshida Y, Kitano H. In vivo robustness analysis of cell division cycle genes in Saccharomyces cerevisiae. PLoS Genet. 2006;2:e111 pubmed
    ..The data were used to evaluate the current computational model, and refinements to the model were suggested. ..

More Information

Publications149 found, 100 shown here

  1. Yang H, Tatebayashi K, Yamamoto K, Saito H. Glycosylation defects activate filamentous growth Kss1 MAPK and inhibit osmoregulatory Hog1 MAPK. EMBO J. 2009;28:1380-91 pubmed publisher
    ..Thus, the reciprocal inhibitory loop between Kss1 and Hog1 allows only one or the other of these MAPKs to be stably activated under various stress conditions. ..
  2. Kawashima S, Yamagishi Y, Honda T, Ishiguro K, Watanabe Y. Phosphorylation of H2A by Bub1 prevents chromosomal instability through localizing shugoshin. Science. 2010;327:172-7 pubmed publisher
    ..Thus, Bub1 kinase creates a mark for shugoshin localization and the correct partitioning of chromosomes. ..
  3. Yamashita M, Kurokawa K, Sato Y, Yamagata A, Mimura H, Yoshikawa A, et al. Structural basis for the Rho- and phosphoinositide-dependent localization of the exocyst subunit Sec3. Nat Struct Mol Biol. 2010;17:180-6 pubmed publisher
    ..To our knowledge, these are the first structural insights into how an exocyst subunit might interact with both protein and phospholipid factors on the target membrane. ..
  4. Furumizu C, Tsukaya H, Komeda Y. Characterization of EMU, the Arabidopsis homolog of the yeast THO complex member HPR1. RNA. 2010;16:1809-17 pubmed publisher
    ..Furthermore, mutations in another THO-related gene led to embryonic lethality. These findings support the presence and importance of the THO-related complex in plants as well as yeast and vertebrates. ..
  5. Hatakeyama R, Kamiya M, Takahara T, Maeda T. Endocytosis of the aspartic acid/glutamic acid transporter Dip5 is triggered by substrate-dependent recruitment of the Rsp5 ubiquitin ligase via the arrestin-like protein Aly2. Mol Cell Biol. 2010;30:5598-607 pubmed publisher
    ..Importantly, the interaction between Aly2 and Dip5 is accelerated in response to elevated aspartic acid availability. This result indicates that the regulation of Dip5 endocytosis is accomplished by dynamic recruitment of Rsp5 via Aly2. ..
  6. Takagi K, Iwamoto K, Kobayashi S, Horiuchi H, Fukuda R, Ohta A. Involvement of Golgi-associated retrograde protein complex in the recycling of the putative Dnf aminophospholipid flippases in yeast. Biochem Biophys Res Commun. 2012;417:490-4 pubmed publisher
    ..Our results suggest that the GARP complex is involved in the recycling of Dnf flippases. ..
  7. Arai T, Noguchi A, Takano E, Kino K. Application of protein N-terminal amidase in enzymatic synthesis of dipeptides containing acidic amino acids specifically at the N-terminus. J Biosci Bioeng. 2013;115:382-7 pubmed publisher
    ..Acidic dipeptide synthesis was examined by a combination of Lals and NTA1, resulting in the synthesis of 12 kinds of Asp-Xaa, including Asp-Phe, a precursor of aspartame, and 11 kinds of Glu-Xaa. ..
  8. Tanigawa M, Maeda T. An In Vitro TORC1 Kinase Assay That Recapitulates the Gtr-Independent Glutamine-Responsive TORC1 Activation Mechanism on Yeast Vacuoles. Mol Cell Biol. 2017;37: pubmed publisher
    ..Thus, this system was proved to be useful for dissecting the glutamine-responsive TORC1 activation mechanism. ..
  9. Morishita T, Mitsuzawa H, Nakafuku M, Nakamura S, Hattori S, Anraku Y. Requirement of Saccharomyces cerevisiae Ras for completion of mitosis. Science. 1995;270:1213-5 pubmed
    ..These results demonstrate that S. cerevisiae Ras functions in the completion of the M phase in a manner independent of the Ras-cAMP pathway. ..
  10. Amakasu H, Suzuki Y, Nishizawa M, Fukasawa T. Isolation and characterization of SGE1: a yeast gene that partially suppresses the gal11 mutation in multiple copies. Genetics. 1993;134:675-83 pubmed
    ..SGE1-specific mRNA of 1.8 kilonucleotides was detected by Northern analysis along the direction of the open reading frame. The gene mapped near RAD56, at the right end of chromosome 16. ..
  11. Muratsubaki H, Enomoto K. One of the fumarate reductase isoenzymes from Saccharomyces cerevisiae is encoded by the OSM1 gene. Arch Biochem Biophys. 1998;352:175-81 pubmed
    ..The N-terminal extension had the characteristics of a typical signal sequence required for targeting and sorting to a noncytosolic destination. In fact, FRDS2 was found to be located in promitochondria. ..
  12. Takeda N, Shibuya M, Maru Y. The BCR-ABL oncoprotein potentially interacts with the xeroderma pigmentosum group B protein. Proc Natl Acad Sci U S A. 1999;96:203-7 pubmed
    ..The persistent dysfunction of XPB may in part underlie genomic instability in blastic crisis. ..
  13. Tone Y, Tanahashi N, Tanaka K, Fujimuro M, Yokosawa H, Toh e A. Nob1p, a new essential protein, associates with the 26S proteasome of growing saccharomyces cerevisiae cells. Gene. 2000;243:37-45 pubmed
    ..These results suggest that association of Nob1p with the proteasomes is essential for the function of the proteasomes in growing cells. ..
  14. Nakanishi H, Nakayama K, Yokota A, Tachikawa H, Takahashi N, Jigami Y. Hut1 proteins identified in Saccharomyces cerevisiae and Schizosaccharomyces pombe are functional homologues involved in the protein-folding process at the endoplasmic reticulum. Yeast. 2001;18:543-54 pubmed
    ..cerevisiae cells. Our results indicate that scHut1p and spHut1p are functional homologues, and their physiological function is to maintain the optimal environment for the folding of secretory pathway proteins in the ER. ..
  15. Tanaka K, Hao Z, Kai M, Okayama H. Establishment and maintenance of sister chromatid cohesion in fission yeast by a unique mechanism. EMBO J. 2001;20:5779-90 pubmed
    ..Eso1p interacts physically with Pds5p via its Ctf7p/Eco1p-homologous domain. ..
  16. Nakanishi T, Sekimizu K. SDT1/SSM1, a multicopy suppressor of S-II null mutant, encodes a novel pyrimidine 5'-nucleotidase. J Biol Chem. 2002;277:22103-6 pubmed
    ..e. 6- or 5-modified UMP. This is the first description of a highly specific pyrimidine 5'-nucleotidase in S. cerevisiae. ..
  17. Kosodo Y, Noda Y, Adachi H, Yoda K. Binding of Sly1 to Sed5 enhances formation of the yeast early Golgi SNARE complex. J Cell Sci. 2002;115:3683-91 pubmed
    ..In vitro formation of this complex on different membranes in yeast lysate was enhanced by the addition of recombinant Sly1. These results indicate that binding of Sly1 to Sed5 enhances trans-SNARE complex formation. ..
  18. Miki H. [Reorganization of the actin cytoskeleton by WASP family proteins]. Seikagaku. 2002;74:1149-61 pubmed
  19. Tatebayashi K, Takekawa M, Saito H. A docking site determining specificity of Pbs2 MAPKK for Ssk2/Ssk22 MAPKKKs in the yeast HOG pathway. EMBO J. 2003;22:3624-34 pubmed
    ..Thus, the docking site contributes to both the efficiency and specificity of signaling. During these analyses, we also found a nuclear export signal and a possible nuclear localization signal in Pbs2. ..
  20. Kim B, Ueda M, Nakamura Y, Kohsaka S, Akazawa C. Expression of the mammalian homologue of vacuolar protein sorting 16 (Vps16p) in the mouse and rat brain. Neurosci Lett. 2004;355:217-20 pubmed
    ..In the primary culture of cortical neurons, mVps16p immunoreactivities were observed in the cell body and the neuronal processes, and highly enriched in the axonal outgrowths. ..
  21. Isono E, Saeki Y, Yokosawa H, Toh e A. Rpn7 Is required for the structural integrity of the 26 S proteasome of Saccharomyces cerevisiae. J Biol Chem. 2004;279:27168-76 pubmed
    ..From these results, we concluded that Rpn7 is required for the integrity of the 26 S complex by establishing a correct lid structure. ..
  22. Hachiya N, Sakasegawa Y, Jozuka A, Tsukita S, Kaneko K. Interaction of D-lactate dehydrogenase protein 2 (Dld2p) with F-actin: implication for an alternative function of Dld2p. Biochem Biophys Res Commun. 2004;319:78-82 pubmed
    ..These properties suggest that Aip2p may interact with F-actin in vivo and play an important role in the yeast cell morphology. ..
  23. Tanida I, Ueno T, Kominami E. Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes. J Biol Chem. 2004;279:47704-10 pubmed
    ..RNA interference of MAP1LC3B mRNA resulted in a decrease in both endogenous MAP1LC3B-PL and MAP1LC3B. These results indicate that the carboxyl terminus of MAP1LC3B is cleaved to expose Gly(120) for further ubiquitylation-like reactions. ..
  24. Noma S, Iida K, Iida H. Polarized morphogenesis regulator Spa2 is required for the function of putative stretch-activated Ca2+-permeable channel component Mid1 in Saccharomyces cerevisiae. Eukaryot Cell. 2005;4:1353-63 pubmed
    ..Therefore, we suggest that Mid1 function is influenced by Spa2 function through polarized morphogenesis. ..
  25. Kodama H, Ito K, Nakamura Y. The role of N-terminal domain of translational release factor eRF3 for the control of functionality and stability in S. cerevisiae. Genes Cells. 2007;12:639-50 pubmed
    ..These findings suggest that NED functions to switch the functional mode of eRF3 depending on the nature of binding factors. ..
  26. Yamamoto E, Yamaguchi S, Sasaki N, Kim H, Kitamori T, Nagamune T. Artificial chaperone-assisted refolding in a microchannel. Bioprocess Biosyst Eng. 2010;33:171-7 pubmed publisher
    ..50%) was approximately three times compared with the simple dilution method. The ACA refolding method is a suitable approach to use in the refolding of proteins using a microfluidic system. ..
  27. Takeuchi Andoh T, Hayano Oshiro Y, Nishiyoshi E, Mutazono M, Hayashi S, Tani T. Saccharomyces cerevisiae MSA1 mRNA has a sequence for localization at the bud tip. Biosci Biotechnol Biochem. 2017;81:1778-1785 pubmed publisher
    ..Msa1p levels were also affected by SHE2 in cells constitutively expressing MSA1 mRNA. These results suggest the existence of a regulatory mechanism for Msa1p through the localized control of MSA1 mRNA. ..
  28. Sakurai H, Kim Y, Ohishi T, Kornberg R, Fukasawa T. The yeast GAL11 protein binds to the transcription factor IIE through GAL11 regions essential for its in vivo function. Proc Natl Acad Sci U S A. 1996;93:9488-92 pubmed
    ..In light of these findings, we suggest that Gal11p stimulates promoter activity by enhancing an association of TFIIE with the preinitiation complex in the cell. ..
  29. Takahashi S, Araki Y, Sakuno T, Katada T. Interaction between Ski7p and Upf1p is required for nonsense-mediated 3'-to-5' mRNA decay in yeast. EMBO J. 2003;22:3951-9 pubmed
    ..These data suggest that the activation of 3'-to-5' directed NMD is mediated through the interaction between Upf1p and the Ski7p N domain. ..
  30. Anraku Y, Satow Y. Reflections on protein splicing: structures, functions and mechanisms. Proc Jpn Acad Ser B Phys Biol Sci. 2009;85:409-21 pubmed
  31. Kang S, Kuzuhara T, Horikoshi M. Functional interaction of general transcription initiation factor TFIIE with general chromatin factor SPT16/CDC68. Genes Cells. 2000;5:251-63 pubmed
    ..This may well lead to a breakthrough in relationships between the transcription initiation process and structural changes in chromatin. ..
  32. Shimoaraiso M, Nakanishi T, Kubo T, Natori S. Transcription elongation factor S-II confers yeast resistance to 6-azauracil by enhancing expression of the SSM1 gene. J Biol Chem. 2000;275:29623-7 pubmed
    ..These results indicate that S-II confers yeast resistance to 6-azauracil by stimulating transcription elongation of the SSM1 gene. ..
  33. Tanida I, Tanida Miyake E, Nishitani T, Komatsu M, Yamazaki H, Ueno T, et al. Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs. Biochem Biophys Res Commun. 2002;292:256-62 pubmed
    ..We also report here on the ubiquitous expression of human APG7 mRNA in human adult and fetal tissues and of rat Apg7p in adult tissues. ..
  34. Komatsu M, Waguri S, Ueno T, Iwata J, Murata S, Tanida I, et al. Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J Cell Biol. 2005;169:425-34 pubmed
    ..Our results indicate the important role of autophagy in starvation response and the quality control of proteins and organelles in quiescent cells...
  35. Nishizawa M, Kawasumi M, Fujino M, Toh e A. Phosphorylation of sic1, a cyclin-dependent kinase (Cdk) inhibitor, by Cdk including Pho85 kinase is required for its prompt degradation. Mol Biol Cell. 1998;9:2393-405 pubmed
    ..Pho85 and other G1 Cdks appear to phosphorylate Sic1 at different sites in vivo. Thus at least two distinct Cdks can participate in phosphorylation of Sic1 and may therefore regulate progression through G1. ..
  36. Watanabe Y, Yokobori S, Inaba T, Yamagishi A, Oshima T, Kawarabayasi Y, et al. Introns in protein-coding genes in Archaea. FEBS Lett. 2002;510:27-30 pubmed
  37. Itoh R, Saint Marc C, Chaignepain S, Katahira R, Schmitter J, Daignan Fornier B. The yeast ISN1 (YOR155c) gene encodes a new type of IMP-specific 5'-nucleotidase. BMC Biochem. 2003;4:4 pubmed
    ..We have identified a new yeast gene, ISN1 (YOR155c), as encoding IMP-specific 5'-nucleotidase activity. The ISN1 gene defines a new type of 5'-nucleotidase which was demonstrated to be functional in vivo. ..
  38. Nishizawa M, Komai T, Morohashi N, Shimizu M, Toh e A. Transcriptional repression by the Pho4 transcription factor controls the timing of SNZ1 expression. Eukaryot Cell. 2008;7:949-57 pubmed publisher
    ..These results imply that Pho4 plays positive and negative roles in transcriptional regulation, with both cases involving structural changes in its target chromatin. ..
  39. Uesono Y, Toh e A, Kikuchi Y, Araki T, Hachiya T, Watanabe C, et al. Local Anesthetics and Antipsychotic Phenothiazines Interact Nonspecifically with Membranes and Inhibit Hexose Transporters in Yeast. Genetics. 2016;202:997-1012 pubmed publisher
    ..When Hxts are scarce, this preference is lost, thereby mitigating the alleviation by additional glucose. These results provide a mechanism that explains how different compounds induce similar effects based on lipid theory. ..
  40. Asakawa K, Toh e A. A defect of Kap104 alleviates the requirement of mitotic exit network gene functions in Saccharomyces cerevisiae. Genetics. 2002;162:1545-56 pubmed
    ..These results suggest that a mutation in Kap104 stimulates exit from mitosis through the activation of Cdc14 and implies a novel role for Kap104 in cell-cycle progression in budding yeast. ..
  41. Hamazaki J, Iemura S, Natsume T, Yashiroda H, Tanaka K, Murata S. A novel proteasome interacting protein recruits the deubiquitinating enzyme UCH37 to 26S proteasomes. EMBO J. 2006;25:4524-36 pubmed
    ..Knockdown of hRpn13 caused no obvious proteolytic defect but loss of UCH37 proteins and decrease in deubiquitinating activity of 26S proteasomes. Our results indicate that hRpn13 is essential for the activity of UCH37. ..
  42. Yashiroda H, Mizushima T, Okamoto K, Kameyama T, Hayashi H, Kishimoto T, et al. Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes. Nat Struct Mol Biol. 2008;15:228-36 pubmed publisher
    ..The structure of the Dmp1-Dmp2-alpha5 complex reveals how this chaperone functions in proteasome assembly and why it dissociates from proteasome precursors before the beta-rings are assembled. ..
  43. Nishizawa M, Tanigawa M, Hayashi M, Maeda T, Yazaki Y, Saeki Y, et al. Pho85 kinase, a cyclin-dependent kinase, regulates nuclear accumulation of the Rim101 transcription factor in the stress response of Saccharomyces cerevisiae. Eukaryot Cell. 2010;9:943-51 pubmed publisher
    ..These findings illustrate novel roles for the regulators of the PHO system when yeast cells cope with various environmental stresses potentially threatening their survival. ..
  44. Ferjani A, Segami S, Horiguchi G, Muto Y, Maeshima M, Tsukaya H. Keep an eye on PPi: the vacuolar-type H+-pyrophosphatase regulates postgerminative development in Arabidopsis. Plant Cell. 2011;23:2895-908 pubmed publisher
    ..Plant cells optimize their metabolic function by eliminating PPi in the cytosol for efficient postembryonic heterotrophic growth. ..
  45. Yamamoto T, Horikoshi M. Novel substrate specificity of the histone acetyltransferase activity of HIV-1-Tat interactive protein Tip60. J Biol Chem. 1997;272:30595-8 pubmed
    ..These results indicate that Tip60 is a histone acetyltransferase with a novel property, suggesting that Tip60 and its related factors may introduce a distinct alteration on chromatin. ..
  46. Sekiya Kawasaki M, Botstein D, Ohya Y. Identification of functional connections between calmodulin and the yeast actin cytoskeleton. Genetics. 1998;150:43-58 pubmed
    ..CAX4 is identical to the ORF YGR036c, and CAX5 is identical to MNN10/SLC2/BED1. We discuss possible roles for Cax proteins in the regulation of the actin cytoskeleton. ..
  47. Nonaka N, Kitajima T, Yokobayashi S, Xiao G, Yamamoto M, Grewal S, et al. Recruitment of cohesin to heterochromatic regions by Swi6/HP1 in fission yeast. Nat Cell Biol. 2002;4:89-93 pubmed
    ..These data define a conserved function of Swi6/HP1 in recruitment of cohesin to heterochromatic regions, promoting the proper segregation of chromosomes. ..
  48. Iiizumi M, Arakawa H, Mori T, Ando A, Nakamura Y. Isolation of a novel gene, CABC1, encoding a mitochondrial protein that is highly homologous to yeast activity of bc1 complex. Cancer Res. 2002;62:1246-50 pubmed
    ..These results suggest that CABC1 may play an important role in mediating p53-inducible apoptosis through the mitochondrial pathway. ..
  49. Sasaki M, Kobayashi T. Ctf4 Prevents Genome Rearrangements by Suppressing DNA Double-Strand Break Formation and Its End Resection at Arrested Replication Forks. Mol Cell. 2017;66:533-545.e5 pubmed publisher
    ..End resection also occurs during physiological rDNA amplification even in the presence of Ctf4. Suppression of end resection is thus important for protecting DSBs at arrested forks from chromosome rearrangements. ..
  50. Nakabayashi J. Optimal ratio of scaffold complex to free Fus3 to maximise the accumulation of phosphorylated Fus3 in yeast pheromone signalling pathway. IET Syst Biol. 2012;6:9-21 pubmed publisher
    ..The proposed model provides further insight into the signalling network from the point of view of not only its function but also its optimality. ..
  51. Kurita T, Noda Y, Yoda K. Action of multiple endoplasmic reticulum chaperon-like proteins is required for proper folding and polarized localization of Kre6 protein essential in yeast cell wall ?-1,6-glucan synthesis. J Biol Chem. 2012;287:17415-24 pubmed publisher
    ..In conclusion, the action of multiple ER chaperon-like proteins is required for proper folding and localization of Kre6 and probably Skn1 to function in ?-1,6-glucan synthesis. ..
  52. Katayama T, Uchida H, Ohta A, Horiuchi H. Involvement of protein kinase C in the suppression of apoptosis and in polarity establishment in Aspergillus nidulans under conditions of heat stress. PLoS ONE. 2012;7:e50503 pubmed publisher
    ..This suggests that PkcA also plays role in polarity establishment during hyphal growth independent of the MAP kinase cascade under these conditions. ..
  53. Ishimi Y, Kikuchi A. Identification and molecular cloning of yeast homolog of nucleosome assembly protein I which facilitates nucleosome assembly in vitro. J Biol Chem. 1991;266:7025-9 pubmed
    ..Proteins recognized by the anti-NAP-I antibody were also present in Xenopus oocytes and Drosophila cultured cells. Possible roles of NAP-I are discussed in relation to other nucleosome assembly proteins. ..
  54. Fujimura H. Molecular cloning of Saccharomyces cerevisiae MGC1/YDR473c gene which is essential for cell growth. Antonie Van Leeuwenhoek. 2000;77:65-9 pubmed
    ..Protein-protein interaction of Mgc1 protein was shown by using yeast two-hybrid system. Mgc1 protein was also proposed to be localized in the nucleus in yeast cells. ..
  55. Hashimoto K, Saito M, Matsuoka H, Iida K, Iida H. Functional analysis of a rice putative voltage-dependent Ca2+ channel, OsTPC1, expressed in yeast cells lacking its homologous gene CCH1. Plant Cell Physiol. 2004;45:496-500 pubmed
    ..5 micro M, was comparable to that of intrinsic CCH1, a candidate VDCC in yeast. Ca(2+) permeation by OsTPC1 was inhibited by verapamil, a VDCC blocker. These findings indicate for the first time that OsTPC1 is a putative VDCC in rice...
  56. Kobayashi T, Funakoshi Y, Hoshino S, Katada T. The GTP-binding release factor eRF3 as a key mediator coupling translation termination to mRNA decay. J Biol Chem. 2004;279:45693-700 pubmed
    ..The termination reaction itself is not sufficient, but eRF3 is essential for triggering mRNA decay. Thus, eRF3 is a key mediator that transduces termination signal to mRNA decay. ..
  57. Onishi M, Iida M, Koga T, Yamada S, Hirata A, Iwaki T, et al. Schizosaccharomyces pombe Sst4p, a conserved Vps27/Hrs homolog, functions downstream of phosphatidylinositol 3-kinase Pik3p to mediate proper spore formation. Eukaryot Cell. 2007;6:2343-53 pubmed
    ..These results suggest that Sst4p is a downstream factor of PtdIns 3-kinase and functions in forespore membrane formation. ..
  58. Takahashi S, Araki Y, Ohya Y, Sakuno T, Hoshino S, Kontani K, et al. Upf1 potentially serves as a RING-related E3 ubiquitin ligase via its association with Upf3 in yeast. RNA. 2008;14:1950-8 pubmed publisher
    ..These results suggest that Upf1 may serve as an E3 ubiquitin ligase upon its association with Upf3 and play an important role in signaling to the NMD pathway. ..
  59. Koyama H, Sumiya E, Nagata M, Ito T, Sekimizu K. Transcriptional repression of the IMD2 gene mediated by the transcriptional co-activator Sub1. Genes Cells. 2008;13:1113-26 pubmed publisher
    ..These results suggest that the transcriptional co-activator Sub1 also has a role in transcriptional repression during transcription initiation in vivo. ..
  60. Nagano M, Ihara Ohori Y, Imai H, Inada N, Fujimoto M, Tsutsumi N, et al. Functional association of cell death suppressor, Arabidopsis Bax inhibitor-1, with fatty acid 2-hydroxylation through cytochrome b?. Plant J. 2009;58:122-34 pubmed publisher
    ..Furthermore, the overexpression of AtBI-1 increased the level of 2-hydroxy fatty acids in Arabidopsis, indicating that AtBI-1 is involved in fatty acid 2-hydroxylation. ..
  61. Ono Y, Fukuda R, Ohta A. Involvement of LEM3/ROS3 in the uptake of phosphatidylcholine with short acyl chains in Saccharomyces cerevisiae. Biosci Biotechnol Biochem. 2009;73:750-2 pubmed
    ..Mutations in LEM3/ROS3 impaired growth in that medium, indicating that Lem3p is involved in the utilization of extracellular phosphatidylcholine (PC) with short acyl chains. ..
  62. Nagamine K, Kudoh J, Minoshima S, Kawasaki K, Asakawa S, Ito F, et al. Genomic organization and complete nucleotide sequence of the human PWP2 gene on chromosome 21. Genomics. 1997;42:528-31 pubmed
    ..3 region, the information including nucleotide sequence and genomic organization of the PWP2 gene should be invaluable for the mutation analysis of the corresponding genetic disorders. ..
  63. Isono E, Saito N, Kamata N, Saeki Y, Toh e A. Functional analysis of Rpn6p, a lid component of the 26 S proteasome, using temperature-sensitive rpn6 mutants of the yeast Saccharomyces cerevisiae. J Biol Chem. 2005;280:6537-47 pubmed
  64. Tomishige N, Noda Y, Adachi H, Shimoi H, Yoda K. SKG1, a suppressor gene of synthetic lethality of kex2Deltagas1Delta mutations, encodes a novel membrane protein that affects cell wall composition. Yeast. 2005;22:141-55 pubmed
    ..Thus SKG1 encodes a novel protein that affects the cell wall polymer composition in the growing region of the cell. ..
  65. Akahane T, Sahara K, Yashiroda H, Tanaka K, Murata S. Involvement of Bag6 and the TRC pathway in proteasome assembly. Nat Commun. 2013;4:2234 pubmed publisher
    ..These findings indicate that proteasome assembly is not solely mediated by dedicated chaperones but also depends on general chaperones that preserve protein homeostasis. ..
  66. Sugimoto N, Tatsumi Y, Tsurumi T, Matsukage A, Kiyono T, Nishitani H, et al. Cdt1 phosphorylation by cyclin A-dependent kinases negatively regulates its function without affecting geminin binding. J Biol Chem. 2004;279:19691-7 pubmed
    ..Taken together, these findings suggest that Cdt1 function is also negatively regulated by the Cdk phosphorylation independent of geminin binding. ..
  67. Kanuka H, Hiratou T, Igaki T, Kanda H, Kuranaga E, Sawamoto K, et al. Gain-of-function screen identifies a role of the Sec61alpha translocon in Drosophila postmitotic neurotoxicity. Biochim Biophys Acta. 2005;1726:225-37 pubmed
  68. Noda Y, Yamagishi T, Yoda K. Specific membrane recruitment of Uso1 protein, the essential endoplasmic reticulum-to-Golgi tethering factor in yeast vesicular transport. J Cell Biochem. 2007;101:686-94 pubmed
    ..The C-terminal acidic region was not required in membrane binding. The presence of membranes to which Uso1 could bind in the yeast cell lysate was detected using the current method. ..
  69. Shinoda J, Kikuchi Y. Rod1, an arrestin-related protein, is phosphorylated by Snf1-kinase in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2007;364:258-63 pubmed
    ..Furthermore, the Rod1 protein was directly phosphorylated by Snf1-kinase in vitro. The substitution of the serine residue 447 to alanine slightly enhanced the resistance to the drug. We discuss possible functions of Rod1. ..
  70. Ishiwata M, Kurahashi H, Nakamura Y. A G-protein gamma subunit mimic is a general antagonist of prion propagation in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2009;106:791-6 pubmed publisher
    ..Assuming the ability of Gpg1 to form G-protein heterotrimeric complexes, Gpg1 is likely to play a versatile function of reversing the prion state and modulating the G-protein signaling pathway. ..
  71. Michimoto T, Aoki T, Toh e A, Kikuchi Y. Yeast Pdr13p and Zuo1p molecular chaperones are new functional Hsp70 and Hsp40 partners. Gene. 2000;257:131-7 pubmed
    ..Taken together, we propose that Pdr13p and Zuo1p are a new pair of Hsp70:Hsp40 molecular chaperones. In addition, Pdr13p co-sedimented with translating ribosomes and this association was independent of the presence of Zuo1p. ..
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