Genomes and Genes
Experts and Doctors on recombinant fusion proteins in Tokyo, Japan
Locale: Tokyo, Japan
Topic: recombinant fusion proteins
Publications261 found, 100 shown here
- Tanida I, Wakabayashi M, Kanematsu T, Minematsu Ikeguchi N, Sou Y, Hirata M, et al. Lysosomal turnover of GABARAP-phospholipid conjugate is activated during differentiation of C2C12 cells to myotubes without inactivation of the mTor kinase-signaling pathway. Autophagy. 2006;2:264-71 pubmed
- Suzuki R, Katayama T, Kitaoka M, Kumagai H, Wakagi T, Shoun H, et al. Crystallographic and mutational analyses of substrate recognition of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum. J Biochem. 2009;146:389-98 pubmed publisher..Our results provide a structural basis for substrate recognition by GH101 endo-alpha-GalNAc-ases and will help structure-based engineering of these enzymes to produce various kinds of neo-glycoconjugates. ..
- Nishino T, Miyaji K, Ishiwata N, Arai K, Yui M, Asai Y, et al. Ex vivo expansion of human hematopoietic stem cells by a small-molecule agonist of c-MPL. Exp Hematol. 2009;37:1364-1377.e4 pubmed publisher..However, their effects on HSCs have not yet been explored. In this study, we evaluated the effects of NR-101, a novel small-molecule c-MPL agonist, on the ex vivo expansion of human cord blood (hCB) HSCs...
- Suzuki E, Daniels T, Helguera G, Penichet M, Umezawa K, Bonavida B. Inhibition of NF-kappaB and Akt pathways by an antibody-avidin fusion protein sensitizes malignant B-cells to cisplatin-induced apoptosis. Int J Oncol. 2010;36:1299-307 pubmed..1Av. Overall, this study provides evidence of the therapeutic potential of ch128.1Av as a chemo-sensitizing agent in drug-resistant tumor cells. ..
- Shiwaku H, Yoshimura N, Tamura T, Sone M, Ogishima S, Watase K, et al. Suppression of the novel ER protein Maxer by mutant ataxin-1 in Bergman glia contributes to non-cell-autonomous toxicity. EMBO J. 2010;29:2446-60 pubmed publisher..Collectively, these results suggest that the reduction of Maxer mediates functional deficiency of Bergmann glia, and might contribute to the non-cell-autonomous pathology of SCA1. ..
- Tsuizaki M, Ohta A, Horiuchi H. Myosin motor-like domain of class VI chitin synthase CsmB of Aspergillus nidulans is not functionally equivalent to that of class V chitin synthase CsmA. Biosci Biotechnol Biochem. 2013;77:369-74 pubmed
- Edamatsu M. Molecular properties of the N-terminal extension of the fission yeast kinesin-5, Cut7. Genet Mol Res. 2016;15: pubmed publisher..These findings suggest that the N-terminal extension is not required for normal Cut7 intracellular localization or function, but alters the microtubule-binding properties of this protein in vitro. ..
- Iwamoto H, Nakamura Y, Konno O, Tomita K, Ueno T, Yokoyama T, et al. Immunosuppressive Therapy for Elderly Kidney Transplant Recipients. Transplant Proc. 2016;48:799-801 pubmed publisher..4%, respectively, while those in the non-elderly group were 98.1% and 92.5%, respectively. Using our current immunosuppressive protocol, the outcomes of patients in the elderly group were considered acceptable. ..
- Marunaka K, Furukawa C, Fujii N, Kimura T, Furuta T, Matsunaga T, et al. The RING finger- and PDZ domain-containing protein PDZRN3 controls localization of the Mg2+ regulator claudin-16 in renal tube epithelial cells. J Biol Chem. 2017;292:13034-13044 pubmed publisher..These results suggest that PDZRN3 mediates endocytosis of dephosphorylated CLDN16 and represents an important component of the CLDN16-trafficking machinery in the kidney. ..
- Morishita K, Sakano K, Takeda K, Maeda S. Characterization of v-sis protein expressed in silkworm larvae using the Bombyx mori nuclear polyhedrosis virus vector. J Biochem. 1991;109:36-44 pubmed..It possessed chemotactic activity but lacked mitogenic activity. In addition, a small amount (approximately 1%) of monomeric v-sis protein was converted in vitro to the mitogenically active v-sis protein, which could be a homo-dimer. ..
- Mimori T, Suganuma K, Tanami Y, Nojima T, Matsumura M, Fujii T, et al. Autoantibodies to calpastatin (an endogenous inhibitor for calcium-dependent neutral protease, calpain) in systemic rheumatic diseases. Proc Natl Acad Sci U S A. 1995;92:7267-71 pubmed..Thus, anti-calpastatin antibodies are present most frequently in patients with RA and may participate in pathogenic mechanisms of rheumatic diseases. ..
- Sano Y, Kobayashi M, Kageyama M. Functional domains of S-type pyocins deduced from chimeric molecules. J Bacteriol. 1993;175:6179-85 pubmed..Pyocins AP41 and S2 have a fourth domain between the receptor-binding and the translocation domains, which is dispensable for their killing functions. ..
- Aoki K, Ishida N, Kawakita M. Substrate recognition by UDP-galactose and CMP-sialic acid transporters. Different sets of transmembrane helices are utilized for the specific recognition of UDP-galactose and CMP-sialic acid. J Biol Chem. 2001;276:21555-61 pubmed
- Yoshikawa N, Makino Y, Okamoto K, Morimoto C, Makino I, Tanaka H. Distinct interaction of cortivazol with the ligand binding domain confers glucocorticoid receptor specificity: cortivazol is a specific ligand for the glucocorticoid receptor. J Biol Chem. 2002;277:5529-40 pubmed..Thus, it is indicated that CVZ may not be only a molecular probe for the analysis of the redundancy between the GR and MR in vivo but also a useful reagent to clarify structure-function relationship of the GR LBD. ..
- Tsuruta F, Masuyama N, Gotoh Y. The phosphatidylinositol 3-kinase (PI3K)-Akt pathway suppresses Bax translocation to mitochondria. J Biol Chem. 2002;277:14040-7 pubmed..Collectively, these results suggest that the PI3K-Akt pathway inhibits Bax translocation from cytoplasm to mitochondria and have revealed a novel mechanism by which the PI3K-Akt pathway promotes survival. ..
- Fukamachi K, Matsuoka Y, Ohno H, Hamaguchi T, Tsuda H. Neuronal leucine-rich repeat protein-3 amplifies MAPK activation by epidermal growth factor through a carboxyl-terminal region containing endocytosis motifs. J Biol Chem. 2002;277:43549-52 pubmed..We propose that NLRR-3 potentiates Ras-MAPK signaling by facilitating internalization of EGF in clathrin-coated vesicles. ..
- Ohara Imaizumi M, Nakamichi Y, Nishiwaki C, Nagamatsu S. Transduction of MIN6 beta cells with TAT-syntaxin SNARE motif inhibits insulin exocytosis in biphasic insulin release in a distinct mechanism analyzed by evanescent wave microscopy. J Biol Chem. 2002;277:50805-11 pubmed..Thus, syntaxin-mediated SNARE assembly modulates insulin exocytosis in biphasic insulin release in a distinct way. ..
- Shinkawa T, Nakamura K, Yamane N, Shoji Hosaka E, Kanda Y, Sakurada M, et al. The absence of fucose but not the presence of galactose or bisecting N-acetylglucosamine of human IgG1 complex-type oligosaccharides shows the critical role of enhancing antibody-dependent cellular cytotoxicity. J Biol Chem. 2003;278:3466-73 pubmed
- Fujise M, Takeo S, Kamimura K, Matsuo T, Aigaki T, Izumi S, et al. Dally regulates Dpp morphogen gradient formation in the Drosophila wing. Development. 2003;130:1515-22 pubmed..In addition, dally affects the shape of the Dpp ligand gradient as well as its activity gradient. We propose that Dally serves as a co-receptor for Dpp and contributes to shaping the Dpp morphogen gradient. ..
- Matsuoka S, Tsurui H, Abe M, Terashima K, Nakamura K, Hamano Y, et al. A monoclonal antibody to the alpha2 domain of murine major histocompatibility complex class I that specifically kills activated lymphocytes and blocks liver damage in the concanavalin A hepatitis model. J Exp Med. 2003;198:497-503 pubmed
- Seimiya H, Muramatsu Y, Smith S, Tsuruo T. Functional subdomain in the ankyrin domain of tankyrase 1 required for poly(ADP-ribosyl)ation of TRF1 and telomere elongation. Mol Cell Biol. 2004;24:1944-55 pubmed..This work provides evidence that specific ARC-TRF1 interactions play roles in the essential catalytic function of tankyrase 1. ..
- Ohira K, Kumanogoh H, Sahara Y, Homma K, Hirai H, Nakamura S, et al. A truncated tropomyosin-related kinase B receptor, T1, regulates glial cell morphology via Rho GDP dissociation inhibitor 1. J Neurosci. 2005;25:1343-53 pubmed..Therefore, these findings indicate that the T1 signaling cascade can alter astrocytic morphology via regulation of Rho GTPase activity. ..
- Hayashi M, Takahashi H, Tamura K, Huang J, Yu L, Kawai Yamada M, et al. Enhanced dihydroflavonol-4-reductase activity and NAD homeostasis leading to cell death tolerance in transgenic rice. Proc Natl Acad Sci U S A. 2005;102:7020-5 pubmed..Thus, a strategy was proposed that the combination of dihydroflavonol-4-reductase activity and the elevated level of NAD(P)H pool may confer the prevention of induced cell death in planta. ..
- Natsume A, Wakitani M, Yamane Ohnuki N, Shoji Hosaka E, Niwa R, Uchida K, et al. Fucose removal from complex-type oligosaccharide enhances the antibody-dependent cellular cytotoxicity of single-gene-encoded antibody comprising a single-chain antibody linked the antibody constant region. J Immunol Methods. 2005;306:93-103 pubmed..These results prove that ADCC enhancement by fucose-removal is effective in not only whole IgG1, but also scFv-Fc, and thus increases the potential of Fc-fusion proteins as therapeutic candidates. ..
- Tsujita K, Suetsugu S, Sasaki N, Furutani M, Oikawa T, Takenawa T. Coordination between the actin cytoskeleton and membrane deformation by a novel membrane tubulation domain of PCH proteins is involved in endocytosis. J Cell Biol. 2006;172:269-79 pubmed..Furthermore, knockdown of endogenous FBP17 and CIP4 impaired endocytosis. Our data indicate that PCH protein family members couple membrane deformation to actin cytoskeleton reorganization in various cellular processes. ..
- Nakajima M, Shimada A, Takashi Y, Kim Y, Park S, Ueguchi Tanaka M, et al. Identification and characterization of Arabidopsis gibberellin receptors. Plant J. 2006;46:880-9 pubmed..The expression of each AtGID1 clone in the rice gid1-1 mutant rescued the GA-insensitive dwarf phenotype. These results demonstrate that all three AtGID1s functioned as GA receptors in Arabidopsis. ..
- Maeda K, Horikoshi T, Nakashima E, Miyamoto Y, Mabuchi A, Ikegawa S. MATN and LAPTM are parts of larger transcription units produced by intergenic splicing: intergenic splicing may be a common phenomenon. DNA Res. 2005;12:365-72 pubmed..Given these findings, the number of gene in the human genome may be smaller than present estimates suggest. ..
- Terauchi K, Asakura T, Ueda H, Tamura T, Tamura K, Matsumoto I, et al. Plant-specific insertions in the soybean aspartic proteinases, soyAP1 and soyAP2, perform different functions of vacuolar targeting. J Plant Physiol. 2006;163:856-62 pubmed..These observations indicated that, in the case of soyAP1, the PSI is not involved in vacuolar targeting, also suggesting that the function of the PSI differs depending on its origin. ..
- Hosokawa N, Sasaki T, Iemura S, Natsume T, Hara T, Mizushima N. Atg101, a novel mammalian autophagy protein interacting with Atg13. Autophagy. 2009;5:973-9 pubmed..Furthermore, Atg101 is important for the stability and basal phosphorylation of Atg13 and ULK1. These data suggest that Atg101 is a novel Atg protein that functions together with ULK, Atg13 and FIP200. ..
- Maruyama Y, Sou Y, Kageyama S, Takahashi T, Ueno T, Tanaka K, et al. LC3B is indispensable for selective autophagy of p62 but not basal autophagy. Biochem Biophys Res Commun. 2014;446:309-15 pubmed publisher..Our results suggest that while mammalian Atg8 homologs are functionally redundant with regard to autophagosome formation, selective autophagy is regulated by specific Atg8 homologs. ..
- Kanai Y, Ochiai A, Shibata T, Oyama T, Ushijima S, Akimoto S, et al. c-erbB-2 gene product directly associates with beta-catenin and plakoglobin. Biochem Biophys Res Commun. 1995;208:1067-72 pubmed..These data suggest that c-erbB-2-induced signaling is also directly liked to the cadherin-mediated cell adhesion and "invasion-suppressor" system through beta-catenin and plakoglobin in cancers. ..
- Shimoaraiso M, Nakanishi T, Kubo T, Natori S. Identification of the region in yeast S-II that defines species specificity in its interaction with RNA polymerase II. J Biol Chem. 1997;272:26550-4 pubmed..This region is known to be less conserved than the N- and C-terminal regions in the S-II family suggesting that it is important in the interaction with transcription machinery proteins in a tissue and/or species-specific manner. ..
- Honda H, Oda H, Suzuki T, Takahashi T, Witte O, Ozawa K, et al. Development of acute lymphoblastic leukemia and myeloproliferative disorder in transgenic mice expressing p210bcr/abl: a novel transgenic model for human Ph1-positive leukemias. Blood. 1998;91:2067-75 pubmed
- Gamou T, Kitamura E, Hosoda F, Shimizu K, Shinohara K, Hayashi Y, et al. The partner gene of AML1 in t(16;21) myeloid malignancies is a novel member of the MTG8(ETO) family. Blood. 1998;91:4028-37 pubmed..Thus, the AML1-MTG16 gene fusion in t(16;21) leukemia results in the production of a protein that is very similar to the AML1-MTG8 chimeric protein. ..
- Okada Y, Hirokawa N. A processive single-headed motor: kinesin superfamily protein KIF1A. Science. 1999;283:1152-7 pubmed..The movement along the microtubules was stochastic and fitted a biased Brownian-movement model. ..
- Kawasaki Y, Senda T, Ishidate T, Koyama R, Morishita T, Iwayama Y, et al. Asef, a link between the tumor suppressor APC and G-protein signaling. Science. 2000;289:1194-7 pubmed..These results suggest that the APC-Asef complex may regulate the actin cytoskeletal network, cell morphology and migration, and neuronal function. ..
- Kurimoto K, Fukai S, Nureki O, Muto Y, Yokoyama S. Crystal structure of human AUH protein, a single-stranded RNA binding homolog of enoyl-CoA hydratase. Structure. 2001;9:1253-63 pubmed..The clefts between the trimers may provide spaces sufficient to accommodate the RNA bases. ..
- Ohneda M, Arioka M, Nakajima H, Kitamoto K. Visualization of vacuoles in Aspergillus oryzae by expression of CPY-EGFP. Fungal Genet Biol. 2002;37:29-38 pubmed..oryzae. Further, visualization of vacuoles revealed the presence of peculiar ring- or tube-like structures as distinct from normal spherical-shaped vacuoles. ..
- Itoh T, Watabe A, Toh e A, Matsui Y. Complex formation with Ypt11p, a rab-type small GTPase, is essential to facilitate the function of Myo2p, a class V myosin, in mitochondrial distribution in Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:7744-57 pubmed..These results indicate that complex formation of Ypt11p with Myo2p accelerates the function of Myo2p for mitochondrial distribution toward the bud. ..
- Kondo T, Kurihara S, Yoshikawa T, Mizukami H. Effect of N- and C-terminal deletions on the RNA N-glycosidase activity and the antigenicity of karasurin-A, a ribosome-inactivating protein from Trichosanthes kirilowii var. japonica. Biotechnol Lett. 2004;26:1873-8 pubmed..The antigenicities of the recombinant proteins were considerably reduced by deleting 20 amino acid residues from either N- or C-terminal regions. ..
- Kawahara M, Inoue T, Ren X, Sogo T, Yamada H, Katoh M, et al. Antigen-mediated growth control of hybridoma cells via a human artificial chromosome. Biochim Biophys Acta. 2007;1770:206-12 pubmed..The AMEGA system in combination with HAC technology will be useful for increasing the efficacy of gene therapy by conferring a growth advantage on the genetically modified cells. ..
- Kondo K, Ochi H, Matsumoto T, Yoshikawa H, Kanda T. Modification of human papillomavirus-like particle vaccine by insertion of the cross-reactive L2-epitopes. J Med Virol. 2008;80:841-6 pubmed publisher..Although the cross-neutralizing titers were lower than the type-specific neutralizing titer, the results suggest that the chimeric VLPs have potential to serve as a vaccine candidate for a broad spectrum of high-risk HPVs. ..
- Masamizu Y, Okada T, Ishibashi H, Takeda S, Yuasa S, Nakahara K. Efficient gene transfer into neurons in monkey brain by adeno-associated virus 8. Neuroreport. 2010;21:447-51 pubmed publisher..The results indicate a preferential tropism of AAV8 for subsets of neurons, but not for glia, in monkey brains. ..
- Hirakawa H, Nagamune T. Nanoscale-engineered cytochrome p450 system with a branch structure. Methods Mol Biol. 2011;743:1-16 pubmed publisher..Because some components of bacterial cytochrome P450 system are interchangeable, this self-sufficient system can be applied to non-natural combination of P450 and electron transfer proteins from different species of bacteria. ..
- Tanigawa M, Kihara A, Terashima M, Takahara T, Maeda T. Sphingolipids regulate the yeast high-osmolarity glycerol response pathway. Mol Cell Biol. 2012;32:2861-70 pubmed publisher..These observations reveal the sphingolipid-mediated regulation of the osmosensing machinery of the HOG pathway...
- Lee S, Takeda Y, Kawano H, Hosoya H, Nomoto M, Fujimoto D, et al. Expression and regulation of a gene encoding neural recognition molecule NB-3 of the contactin/F3 subgroup in mouse brain. Gene. 2000;245:253-66 pubmed..2kb fragment upstream of the putative transcription initiation site. This study provides a basis for elucidating the biological significance of the contactin/F3 subgroup molecules. ..
- Yamamoto A, Hashimoto Y, Kohri K, Ogata E, Kato S, Ikeda K, et al. Cyclin E as a coactivator of the androgen receptor. J Cell Biol. 2000;150:873-80 pubmed..These results suggest that cyclin E functions as a coactivator of the AR, and that aberrant expression of cyclin E in tumors may contribute to persistent activation of AR function, even during androgen ablation therapy. ..
- Uchida S, Tanaka Y, Ito H, Saitoh Ohara F, Inazawa J, Yokoyama K, et al. Transcriptional regulation of the CLC-K1 promoter by myc-associated zinc finger protein and kidney-enriched Krüppel-like factor, a novel zinc finger repressor. Mol Cell Biol. 2000;20:7319-31 pubmed..These results suggest that a novel set of zinc finger proteins may help regulate the strict tissue- and nephron segment-specific expression of the CLC-K1 and CLC-K2 channel genes through their GA cis element. ..
- Inokuchi K, Yamaguchi H, Tarusawa M, Futaki M, Hanawa H, Tanosaki S, et al. Abnormality of c-kit oncoprotein in certain patients with chronic myelogenous leukemia--potential clinical significance. Leukemia. 2002;16:170-7 pubmed..These mutations and in vitro functional analyses raise the possibility that the KIT abnormalities influence the white blood cell counts (P < 0.05) and survival (P < 0.04) of CML patients. ..
- Takeshita N, Ohta A, Horiuchi H. csmA, a gene encoding a class V chitin synthase with a myosin motor-like domain of Aspergillus nidulans, is translated as a single polypeptide and regulated in response to osmotic conditions. Biochem Biophys Res Commun. 2002;298:103-9 pubmed
- Hara H, Nakayashiki T, Crist C, Nakamura Y. Prion domain interaction responsible for species discrimination in yeast [PSI+] transmission. Genes Cells. 2003;8:925-39 pubmed..NR as well as NQ alone coalesces into existing [PSI+] aggregates, showing their independent potentials to interact with the identical sequence in the [PSI+] conformer. The role of NQ and NR in [PSI+] prion formation is discussed. ..
- Kim Y, Sato K, Asagiri M, Morita I, Soma K, Takayanagi H. Contribution of nuclear factor of activated T cells c1 to the transcriptional control of immunoreceptor osteoclast-associated receptor but not triggering receptor expressed by myeloid cells-2 during osteoclastogenesis. J Biol Chem. 2005;280:32905-13 pubmed..These mechanisms, possibly together with the delicate regulation of their ligands on osteoblasts, may provide the exquisite machinery for the modulation of osteoclastogenesis in the maintenance of bone homeostasis. ..
- Nakano K, Arai R, Mabuchi I. Small GTPase Rho5 is a functional homologue of Rho1, which controls cell shape and septation in fission yeast. FEBS Lett. 2005;579:5181-6 pubmed..Thus, it is likely that Rho5 has an overlapping function with Rho1 in controlling cell growth and division in S. pombe. ..
- Kato A, Yamamoto M, Ohno T, Tanaka M, Sata T, Nishiyama Y, et al. Herpes simplex virus 1-encoded protein kinase UL13 phosphorylates viral Us3 protein kinase and regulates nuclear localization of viral envelopment factors UL34 and UL31. J Virol. 2006;80:1476-86 pubmed publisher..These results indicate that UL13 phosphorylates Us3 in infected cells and regulates UL34 and UL31 localization, either by phosphorylating Us3 or by a Us3-independent mechanism...
- Ito T, Saso K, Arimitsu N, Sekimizu K. Defective FESTA/EAF2-mediated transcriptional activation in S-II-deficient embryonic stem cells. Biochem Biophys Res Commun. 2007;363:603-9 pubmed..These results suggest that S-II is selectively required for positive transcriptional regulation of a subset of genes in murine ES cells. ..
- Asai R, Ohno T, Kato A, Kawaguchi Y. Identification of proteins directly phosphorylated by UL13 protein kinase from herpes simplex virus 1. Microbes Infect. 2007;9:1434-8 pubmed..We also identified UL41 as a previously unreported and novel substrate of UL13. These data will serve as a basis to clarify the mechanism by which UL13 influences viral replication. ..
- Kohsaka H, Takasu E, Nose A. In vivo induction of postsynaptic molecular assembly by the cell adhesion molecule Fasciclin2. J Cell Biol. 2007;179:1289-300 pubmed..We propose that Fas2 mediates trans-synaptic adhesion, which contributes to postsynaptic molecular assembly at the onset of synaptogenesis. ..
- Murakami Y, Tatebayashi K, Saito H. Two adjacent docking sites in the yeast Hog1 mitogen-activated protein (MAP) kinase differentially interact with the Pbs2 MAP kinase kinase and the Ptp2 protein tyrosine phosphatase. Mol Cell Biol. 2008;28:2481-94 pubmed publisher..Thus, the CD and the PBD-2 docking sites play critical roles in both the activation and inactivation of Hog1. ..
- Urakami E, Yamaguchi I, Asami T, Conrad U, Suzuki Y. Immunomodulation of gibberellin biosynthesis using an anti-precursor gibberellin antibody confers gibberellin-deficient phenotypes. Planta. 2008;228:863-73 pubmed publisher..These results demonstrate that the scFv trapped GA(24) in ER and inhibited metabolism of GA(24) to bioactive GA(4). ..
- Zhou S, Fushinobu S, Kim S, Nakanishi Y, Maruyama J, Kitamoto K, et al. Functional analysis and subcellular location of two flavohemoglobins from Aspergillus oryzae. Fungal Genet Biol. 2011;48:200-7 pubmed publisher..These results suggest that FHb1 and FHb2 may play a role in protecting cells from external and internal NO stress, respectively...
- Nishimoto N, Arai S, Ichikawa M, Nakagawa M, Goyama S, Kumano K, et al. Loss of AML1/Runx1 accelerates the development of MLL-ENL leukemia through down-regulation of p19ARF. Blood. 2011;118:2541-50 pubmed publisher..These results provide a new insight into a role for AML1 in the progression of leukemia. ..
- Hoshi M, Ohki Y, Ito K, Tomita T, Iwatsubo T, Ishimaru Y, et al. Experimental detection of proteolytic activity in a signal peptide peptidase of Arabidopsis thaliana. BMC Biochem. 2013;14:16 pubmed publisher..We concluded that plant SPP possesses proteolytic activity and may be involved in RIP. ..
- Ishiwatari Hayasaka H, Maruya M, Sreedhar A, Nemoto T, Csermely P, Yahara I. Interaction of neuropeptide Y and Hsp90 through a novel peptide binding region. Biochemistry. 2003;42:12972-80 pubmed..Mutation of arginine residues in NPY13-36 to alanine abrogated binding to Hsp90. Our studies indicate that the hinge region after the N-terminal domain of Hsp90 and the positive charges on NPY are important for this interaction. ..
- Tsukamoto S, Kuma A, Murakami M, Kishi C, Yamamoto A, Mizushima N. Autophagy is essential for preimplantation development of mouse embryos. Science. 2008;321:117-20 pubmed publisher..Protein synthesis rates were reduced in the autophagy-null embryos. Thus, autophagic degradation within early embryos is essential for preimplantation development in mammals. ..
- Hirata J, Nakagoshi H, Nabeshima Y, Matsuzaki F. Asymmetric segregation of the homeodomain protein Prospero during Drosophila development. Nature. 1995;377:627-30 pubmed..We propose that asymmetric segregation of transcription factors is an intrinsic mechanism for establishing asymmetry in gene expression between sibling cells. ..
- Ishikawa H, Tachikawa H, Miura Y, Takahashi N. TRIM11 binds to and destabilizes a key component of the activator-mediated cofactor complex (ARC105) through the ubiquitin-proteasome system. FEBS Lett. 2006;580:4784-92 pubmed..These results suggest that TRIM11, with the ubiquitin-proteasome pathway, regulates ARC105 function in TGFbeta signaling. ..
- Nakaoka H, Wakamoto Y. Aging, mortality, and the fast growth trade-off of Schizosaccharomyces pombe. PLoS Biol. 2017;15:e2001109 pubmed publisher..pombe and thus cannot be an appropriate molecular marker or index for replicative aging under both favorable and stressful environmental conditions. ..
- Suenaga N, Mori H, Itoh Y, Seiki M. CD44 binding through the hemopexin-like domain is critical for its shedding by membrane-type 1 matrix metalloproteinase. Oncogene. 2005;24:859-68 pubmed..However, the shedding activity was different depending on the catalytic domains. The conserved binding ability of the HPX domains suggests that CD44H may act as a core molecule assembling multiple MT-MMPs on the cell surface. ..
- Ogawa T, Pan L, Kawai Yamada M, Yu L, Yamamura S, Koyama T, et al. Functional analysis of Arabidopsis ethylene-responsive element binding protein conferring resistance to Bax and abiotic stress-induced plant cell death. Plant Physiol. 2005;138:1436-45 pubmed..Up-regulation of defense genes was observed in transgenic Arabidopsis plants overexpressing AtEBP. Based on the analysis of mRNA accumulation in ethylene-related mutants, the position of AtEBP in signaling pathway is presented. ..
- Ohbayashi I, Konishi M, Ebine K, Sugiyama M. Genetic identification of Arabidopsis RID2 as an essential factor involved in pre-rRNA processing. Plant J. 2011;67:49-60 pubmed publisher..It can be inferred from these findings that RID2 contributes to the nucleolar activity for pre-rRNA processing, probably through some methylation reaction. ..
- Shimura H, Hattori N, Kubo S, Mizuno Y, Asakawa S, Minoshima S, et al. Familial Parkinson disease gene product, parkin, is a ubiquitin-protein ligase. Nat Genet. 2000;25:302-5 pubmed
- Bandoh K, Aoki J, Taira A, Tsujimoto M, Arai H, Inoue K. Lysophosphatidic acid (LPA) receptors of the EDG family are differentially activated by LPA species. Structure-activity relationship of cloned LPA receptors. FEBS Lett. 2000;478:159-65 pubmed..In conclusion, the present study demonstrates that each LPA receptor can be activated differentially by the LPA species. ..
- Mori H, Tomari T, Koshikawa N, Kajita M, Itoh Y, Sato H, et al. CD44 directs membrane-type 1 matrix metalloproteinase to lamellipodia by associating with its hemopexin-like domain. EMBO J. 2002;21:3949-59 pubmed..The PEX domain of MT1-MMP was indispensable in promoting cell migration and CD44H shedding. ..
- Hu Q, Saegusa H, Hayashi Y, Tanabe T. The carboxy-terminal tail region of human Cav2.1 (P/Q-type) channel is not an essential determinant for its subcellular localization in cultured neurones. Genes Cells. 2005;10:87-96 pubmed..Thus, it is possible that the localization mechanism of Ca(v)2.1 channel is different from that of Ca(v)2.2, though these channels share various structural and functional characteristics. ..
- Matsuda K, Miura E, Miyazaki T, Kakegawa W, Emi K, Narumi S, et al. Cbln1 is a ligand for an orphan glutamate receptor delta2, a bidirectional synapse organizer. Science. 2010;328:363-8 pubmed publisher..These results indicate that the Cbln1-GluD2 complex is a unique synapse organizer that acts bidirectionally on both pre- and postsynaptic components. ..
- Mizuguchi R, Hatakeyama M. Conditional activation of Janus kinase (JAK) confers factor independence upon interleukin-3-dependent cells. Essential role of Ras in JAK-triggered mitogenesis. J Biol Chem. 1998;273:32297-303 pubmed..These results indicate that activation of JAK through membrane-proximal dimerization is sufficient to induce cell cycle progression and that Ras is essentially involved in JAK-triggered mitogenesis. ..
- Takahashi Y, Tanaka Y, Yamashita A, Koyanagi Y, Nakamura M, Yamamoto N. OX40 stimulation by gp34/OX40 ligand enhances productive human immunodeficiency virus type 1 infection. J Virol. 2001;75:6748-57 pubmed
- Jishage M, Fujino T, Yamazaki Y, Kuroda H, Nakamura T. Identification of target genes for EWS/ATF-1 chimeric transcription factor. Oncogene. 2003;22:41-9 pubmed..Moreover, induction of POSH brought apoptotic cell death to KAS, the clear cell sarcoma (CCS) cell line, suggesting that repressed expression of POSH in CCS may be relevant to the normal signaling pathway in apoptosis. ..
- Katane M, Furuchi T, Sekine M, Homma H. Molecular cloning of a cDNA encoding mouse D-aspartate oxidase and functional characterization of its recombinant proteins by site-directed mutagenesis. Amino Acids. 2007;32:69-78 pubmed..These results suggest that the Arg-216 and Arg-237 residues of mDASPO are catalytically important for full enzyme activity. ..
- Uchida T, Suzuki K, Hayashi N, Iida F, Hara T, Oo S, et al. Hepatitis E virus: cDNA cloning and expression. Microbiol Immunol. 1992;36:67-79 pubmed..The data indicate that the cDNA fragments are useful for immunodiagnosis of hepatitis E. ..
- Yamauchi J, Miyamoto Y, Sanbe A, Tanoue A. JNK phosphorylation of paxillin, acting through the Rac1 and Cdc42 signaling cascade, mediates neurite extension in N1E-115 cells. Exp Cell Res. 2006;312:2954-61 pubmed
- Fujimoto M, Arimura S, Nakazono M, Tsutsumi N. Arabidopsis dynamin-related protein DRP2B is co-localized with DRP1A on the leading edge of the forming cell plate. Plant Cell Rep. 2008;27:1581-6 pubmed publisher..These results indicate that Arabidopsis dynamin-related proteins, DRP1A and DRP2B, from two different families, participate in membrane remodeling at a similar place in the cell plate. ..
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- Sagou K, Uema M, Kawaguchi Y. Nucleolin is required for efficient nuclear egress of herpes simplex virus type 1 nucleocapsids. J Virol. 2010;84:2110-21 pubmed publisher..These results indicated that nucleolin is a cellular factor required for efficient nuclear egress of HSV-1 nucleocapsids in infected cells. ..