Experts and Doctors on flow cytometry in Tokushima, Japan


Locale: Tokushima, Japan
Topic: flow cytometry

Top Publications

  1. Yoshioka M, Fukuishi N, Kubo Y, Yamanobe H, Ohsaki K, Kawasoe Y, et al. Human cathelicidin CAP18/LL-37 changes mast cell function toward innate immunity. Biol Pharm Bull. 2008;31:212-6 pubmed
    ..In conclusion, LL-37 may be a candidate modifier of the host defense against bacterial entry by serving as an alarm for sentinels such as mast cells. ..
  2. Abe T, Hirasaka K, Kagawa S, Kohno S, Ochi A, Utsunomiya K, et al. Cbl-b is a critical regulator of macrophage activation associated with obesity-induced insulin resistance in mice. Diabetes. 2013;62:1957-69 pubmed publisher
    ..Therefore, inhibition of TLR4 signaling is an attractive therapeutic strategy for treatment of obesity-related insulin resistance. ..
  3. Ishimaru N, Takagi A, Kohashi M, Yamada A, Arakaki R, Kanno J, et al. Neonatal exposure to low-dose 2,3,7,8-tetrachlorodibenzo-p-dioxin causes autoimmunity due to the disruption of T cell tolerance. J Immunol. 2009;182:6576-86 pubmed publisher
    ..These results suggest that TCDD/aryl hydrocarbon receptor signaling in the neonatal thymus plays an important role in the early thymic differentiation related to autoimmunity. ..
  4. Ueha Ishibashi T, Oyama Y, Nakao H, Umebayashi C, Nishizaki Y, Tatsuishi T, et al. Effect of thimerosal, a preservative in vaccines, on intracellular Ca2+ concentration of rat cerebellar neurons. Toxicology. 2004;195:77-84 pubmed
    ..Results indicate that thimerosal exerts some cytotoxic actions on cerebellar granule neurons dissociated from 2-week-old rats and its potency is almost similar to that of methylmercury. ..
  5. Ueha Ishibashi T, Oyama Y, Nakao H, Umebayashi C, Hirama S, Sakai Y, et al. Flow-cytometric analysis on cytotoxic effect of thimerosal, a preservative in vaccines, on lymphocytes dissociated from rat thymic glands. Toxicol In Vitro. 2005;19:191-8 pubmed
    ..Since the blood concentration of thimerosal after receiving vaccines is theoretically submicromolar, it may be unlikely that thimerosal affects lymphocytes of infants. ..
  6. Tochizawa S, Ohmoto Y, Mori T. A novel modification of a flow cytometric assay of phosphorylated STAT1 in whole blood lymphocytes for rapid detection of interferon-alpha signal in vivo. J Immunol Methods. 2006;313:29-37 pubmed
    ..This new flow cytometric assay of p-STAT1 in peripheral blood leukocytes will be useful for examining IFN-alpha signaling and for monitoring tissue response to IFN-alpha in vivo. ..
  7. Ishimaru N, Yamada A, Kohashi M, Arakaki R, Takahashi T, Izumi K, et al. Development of inflammatory bowel disease in Long-Evans Cinnamon rats based on CD4+CD25+Foxp3+ regulatory T cell dysfunction. J Immunol. 2008;180:6997-7008 pubmed
  8. Matsumori Y, Yano S, Goto H, Nakataki E, Wedge S, Ryan A, et al. ZD6474, an inhibitor of vascular endothelial growth factor receptor tyrosine kinase, inhibits growth of experimental lung metastasis and production of malignant pleural effusions in a non-small cell lung cancer model. Oncol Res. 2006;16:15-26 pubmed
    ..These results indicate that ZD6474, an inhibitor of VEGFR-2, may be useful in controlling the growth of established lung metastasis and pleural effusions by NSCLC. ..
  9. Horimoto K, Nishimura Y, Oyama T, Onoda K, Matsui H, Oyama T, et al. Reciprocal effects of glucose on the process of cell death induced by calcium ionophore or H2O2 in rat lymphocytes. Toxicology. 2006;225:97-108 pubmed
    ..The order in the reactivity cannot explain the protective action of glucose. Glucose at high concentrations exerts reciprocal actions on the process of cell death induced by the oxidative stress and excessive increase in [Ca(2+)](i). ..

More Information


  1. Nakamura M, Ishimura K. Size-controlled, one-pot synthesis, characterization, and biological applications of epoxy-organosilica particles possessing positive zeta potential. Langmuir. 2008;24:12228-34 pubmed publisher
    ..We also discuss the advantages of EpoMS particles, as well as the potential biological applications of these particles. ..
  2. Kagawa K, Nakano A, Miki H, Oda A, Amou H, Takeuchi K, et al. Inhibition of TACE activity enhances the susceptibility of myeloma cells to TRAIL. PLoS ONE. 2012;7:e31594 pubmed publisher
    ..It can be envisaged that TACE inhibition augments clinical efficacy of TRAIL-based immunotherapy against MM, which eventually becomes resistant to the present therapeutic modalities. ..
  3. Wang W, Nishioka Y, Ozaki S, Jalili A, Abe S, Kakiuchi S, et al. HM1.24 (CD317) is a novel target against lung cancer for immunotherapy using anti-HM1.24 antibody. Cancer Immunol Immunother. 2009;58:967-76 pubmed publisher
    ..24 antibody showed the enhanced antitumor effects even in the delayed treatment schedule. HM1.24 antigen is a novel immunological target for the treatment of lung cancer with anti-HM1.24 antibody. ..
  4. Oyama T, Ishida S, Okano Y, Seo H, Oyama Y. Clioquinol-induced increase and decrease in the intracellular Zn2+ level in rat thymocytes. Life Sci. 2012;91:1216-20 pubmed publisher
    ..The effect of clioquinol on the intracellular Zn(2+) level varies, depending on the extracellular Zn(2+) concentration and the clioquinol concentration. Clioquinol may therefore exert various types of Zn(2+)-dependent cytotoxicity. ..
  5. Nakano A, Tsuji D, Miki H, Cui Q, El Sayed S, Ikegame A, et al. Glycolysis inhibition inactivates ABC transporters to restore drug sensitivity in malignant cells. PLoS ONE. 2011;6:e27222 pubmed publisher
  6. Ikemoto T, Shimada M, Komatsu M, Yamada S, Saito Y, Mori H, et al. Indoleamine 2,3-dioxygenase affects the aggressiveness of intraductal papillary mucinous neoplasms through Foxp3+CD4+CD25+ T cells in peripheral blood. Pancreas. 2013;42:130-4 pubmed publisher
    ..01, log-rank test). Peripheral Foxp3(+) Tregs accurately reflect the aggressiveness of IPMNs. An increase in Foxp3(+) Tregs can be induced by local IDO-positive cells in IPMN. ..
  7. Sakai T, Kogiso M, Mitsuya K, Komatsu T, Yamamoto S. Genistein enhances antigen-specific cytokine production in female DO11.10 transgenic mice. J Nutr Sci Vitaminol (Tokyo). 2006;52:327-32 pubmed
    ..In contrast to the female mice, genistein did not increase cytokine production in male mice, suggesting that the effect of genistein on cytokine production is gender-dependent. ..
  8. Ueha Ishibashi T, Tatsuishi T, Iwase K, Nakao H, Umebayashi C, Nishizaki Y, et al. Property of thimerosal-induced decrease in cellular content of glutathione in rat thymocytes: a flow cytometric study with 5-chloromethylfluorescein diacetate. Toxicol In Vitro. 2004;18:563-9 pubmed
  9. Cossarizza A, Chang H, Radbruch A, Akdis M, Andrä I, Annunziato F, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies. Eur J Immunol. 2017;47:1584-1797 pubmed publisher
  10. Arakaki R, Nagaoka A, Ishimaru N, Yamada A, Yoshida S, Hayashi Y. Role of plasmacytoid dendritic cells for aberrant class II expression in exocrine glands from estrogen-deficient mice of healthy background. Am J Pathol. 2009;174:1715-24 pubmed publisher
  11. Ohigashi I, Zuklys S, Sakata M, Mayer C, Zhanybekova S, Murata S, et al. Aire-expressing thymic medullary epithelial cells originate from ?5t-expressing progenitor cells. Proc Natl Acad Sci U S A. 2013;110:9885-90 pubmed publisher
    ..These results indicate that the majority of mTECs, including autoimmune regulator-expressing mTECs, are derived from ?5t-expressing progenitor cells...
  12. Abe S, Morita Y, Kaneko M, Hanibuchi M, Tsujimoto Y, Goto H, et al. A novel targeting therapy of malignant mesothelioma using anti-podoplanin antibody. J Immunol. 2013;190:6239-49 pubmed publisher
    ..These results strongly suggest that targeting therapy to podoplanin with therapeutic Abs (i.e., NZ-8) derived from NZ-1 might be useful as a novel immunotherapy against MPM...
  13. Ashrin M, Arakaki R, Yamada A, Kondo T, Kurosawa M, Kudo Y, et al. A critical role for thymic stromal lymphopoietin in nickel-induced allergy in mice. J Immunol. 2014;192:4025-31 pubmed publisher
    ..These results suggest that Ni allergy may be triggered by a TSLP/TSLPR-mediated interaction between epithelial and immune cells. ..
  14. Kozai M, Kubo Y, Katakai T, Kondo H, Kiyonari H, Schaeuble K, et al. Essential role of CCL21 in establishment of central self-tolerance in T cells. J Exp Med. 2017;214:1925-1935 pubmed publisher
    ..These results indicate a nonredundant role of CCL21Ser in the establishment of self-tolerance in T cells in the thymic medulla, and reveal a functional inequality among CCR7 ligands in vivo. ..
  15. Ikemoto T, Shimada M, Ishikawa D, Kawashita Y, Teraoku H, Yoshikawa M, et al. Peripheral Tr1 and Foxp3+ Treg as Markers of Recurrent Malignancies in Patients with Hepato-Biliary Pancreatic Cancers. Anticancer Res. 2017;37:5541-5552 pubmed
    ..Thus, this study aimed to investigate whether Treg and Tr1 cells participate in immunological status against cancer...
  16. Hosokawa Y, Nakanishi T, Yamaguchi D, Takahashi K, Yumoto H, Ozaki K, et al. Macrophage inflammatory protein 3alpha-CC chemokine receptor 6 interactions play an important role in CD4+ T-cell accumulation in periodontal diseased tissue. Clin Exp Immunol. 2002;128:548-54 pubmed
    ..Thus, these findings suggested that CCR6 and the corresponding chemokine, MIP-3alpha may have an important regulatory role in specific lymphocyte migration into inflamed periodontal tissue. ..
  17. Tamura I, Kanbara Y, Saito M, Horimoto K, Satoh M, Yamamoto H, et al. Triclosan, an antibacterial agent, increases intracellular Zn(2+) concentration in rat thymocytes: its relation to oxidative stress. Chemosphere. 2012;86:70-5 pubmed publisher
    ..Since recent studies show many physiological roles of intracellular Zn(2+) in cellular functions, the triclosan-induced disturbance of cellular Zn(2+) homeostasis may induce adverse actions on the cells. ..
  18. Ishifune C, Maekawa Y, Nishida J, Kitamura A, Tanigaki K, Yagita H, et al. Notch signaling regulates the development of a novel type of Thy1-expressing dendritic cell in the thymus. Eur J Immunol. 2011;41:1309-20 pubmed publisher
    ..Furthermore, our findings suggest that Notch signaling regulates T-cell development not only by intrinsically inducing T-cell lineage-specific gene programs, but also by regulating negative selection through Thy1(+) DCs. ..
  19. Okazaki T, Okazaki I, Wang J, Sugiura D, Nakaki F, Yoshida T, et al. PD-1 and LAG-3 inhibitory co-receptors act synergistically to prevent autoimmunity in mice. J Exp Med. 2011;208:395-407 pubmed publisher
    ..In addition, LAG-3 deficiency alone accelerated type 1 diabetes mellitus in nonobese diabetic mice. These results demonstrate that LAG-3 acts synergistically with PD-1 and/or other immunoregulatory genes to prevent autoimmunity in mice. ..
  20. Saha A, Kuzuhara T, Echigo N, Fujii A, Suganuma M, Fujiki H. Apoptosis of human lung cancer cells by curcumin mediated through up-regulation of "growth arrest and DNA damage inducible genes 45 and 153". Biol Pharm Bull. 2010;33:1291-9 pubmed
    ..All the results with PC-9 cells suggest that the up-regulation of GADD45 and 153 by curcumin is a prime mechanism in the anticancer activity of curcumin. ..
  21. Yan H, Shinka T, Sato Y, Yang X, Chen G, Sakamoto K, et al. Overexpression of SOX15 inhibits proliferation of NT2/D1 cells derived from a testicular embryonal cell carcinoma. Mol Cells. 2007;24:323-8 pubmed
    ..Among the up-regulated genes, IGFBP5, S100A4, ID2, FABP5, MTSS1, PDCD4 have been shown to be related to cell proliferation and/or the cell cycle. ..
  22. Nakamura M, Shono M, Ishimura K. Synthesis, characterization, and biological applications of multifluorescent silica nanoparticles. Anal Chem. 2007;79:6507-14 pubmed
    ..We discuss the advantages and demonstrate the usefulness of our nanoparticles in relation to commercially available fluorescent nanoparticles including quantum dots. ..
  23. Hirasaka K, Kohno S, Goto J, Furochi H, Mawatari K, Harada N, et al. Deficiency of Cbl-b gene enhances infiltration and activation of macrophages in adipose tissue and causes peripheral insulin resistance in mice. Diabetes. 2007;56:2511-22 pubmed
    ..Cbl-b is a negative regulator of macrophage infiltration and activation, and macrophage activation by Cbl-b deficiency contributes to the peripheral insulin resistance and glucose intolerance via cytokines secreted from macrophages. ..
  24. Saegusa K, Ishimaru N, Yanagi K, Haneji N, Nishino M, Azuma M, et al. Autoantigen-specific CD4+CD28low T cell subset prevents autoimmune exocrinopathy in murine Sjögren's syndrome. J Immunol. 2000;165:2251-7 pubmed
    ..These results suggest that a CD4+CD28low T cell subset that is continuously activated by an organ-specific autoantigen may play a regulatory role in the development of organ-specific autoimmune disease in an animal model of SS. ..
  25. Kawanai T. Triclosan, an environmental pollutant from health care products, evokes charybdotoxin-sensitive hyperpolarization in rat thymocytes. Environ Toxicol Pharmacol. 2011;32:417-22 pubmed publisher
    ..Since the change in membrane potential of lymphocytes influence cellular immune functions, triclosan may exert adverse actions on immune system in human and wild animals. ..
  26. Mouri Y, Yano M, Shinzawa M, Shimo Y, Hirota F, Nishikawa Y, et al. Lymphotoxin signal promotes thymic organogenesis by eliciting RANK expression in the embryonic thymic stroma. J Immunol. 2011;186:5047-57 pubmed publisher
    ..Thus, the LT?R signal exhibits a novel and unique function in promoting RANK activity for mTEC organization, indicating a link between thymic organogenesis mediated by multiple cytokine signals and the control of autoimmunity. ..
  27. Lei Y, Ripen A, Ishimaru N, Ohigashi I, Nagasawa T, Jeker L, et al. Aire-dependent production of XCL1 mediates medullary accumulation of thymic dendritic cells and contributes to regulatory T cell development. J Exp Med. 2011;208:383-94 pubmed publisher
    ..These results indicate that the XCL1-mediated medullary accumulation of tDCs contributes to nT reg cell development and is regulated by Aire. ..
  28. Uchida D, Kuribayashi N, Kinouchi M, Ohe G, Tamatani T, Nagai H, et al. Expression and function of CXCR4 in human salivary gland cancers. Clin Exp Metastasis. 2013;30:133-42 pubmed publisher
    ..These results indicate that CXCR4 expression contributes to the metastatic potential of SGCs. ..
  29. Izawa T, Kondo T, Kurosawa M, Oura R, Matsumoto K, Tanaka E, et al. Fas-independent T-cell apoptosis by dendritic cells controls autoimmune arthritis in MRL/lpr mice. PLoS ONE. 2012;7:e48798 pubmed publisher
    ..These results strongly suggest that a novel Fas-independent apoptosis pathway in T cells maintains peripheral tolerance and thus controls autoimmunity in MRL/lpr mice. ..
  30. Nakanishi T, Mukai K, Yumoto H, Hirao K, Hosokawa Y, Matsuo T. Anti-inflammatory effect of catechin on cultured human dental pulp cells affected by bacteria-derived factors. Eur J Oral Sci. 2010;118:145-50 pubmed publisher
    ..These findings suggest that green tea catechins may prevent the exacerbation of pulpitis. ..
  31. Uchida D, Onoue T, Begum N, Kuribayashi N, Tomizuka Y, Tamatani T, et al. Vesnarinone downregulates CXCR4 expression via upregulation of Krüppel-like factor 2 in oral cancer cells. Mol Cancer. 2009;8:62 pubmed publisher
    ..The use of siRNA against KLF2 led to an upregulation of CXCR4 mRNA. These Results indicate that vesnarinone downregulates CXCR4 via the upregulation of KLF2 in oral cancer. ..
  32. Sakai T, Liu L, Teng X, Ishimaru N, Mukai Sakai R, Tran N, et al. Inflammatory disease and cancer with a decrease in Kupffer cell numbers in Nucling-knockout mice. Int J Cancer. 2010;126:1079-94 pubmed publisher
    ..We propose that Nucling deficiency could be a powerful tool to reveal the NF-kappaB-related molecular networks leading to hepatitis and HCC development. ..