Experts and Doctors on cricetulus in Hiroshima, Japan
Locale: Hiroshima, Japan
Shimizu N, Hanada N, Utani K, Sekiguchi N. Interconversion of intra- and extra-chromosomal sites of gene amplification by modulation of gene expression and DNA methylation. J Cell Biochem. 2007;102:515-29 pubmed
..These results have important implications for both protein production technology, and the generation of chromosomal abnormalities found in human cancer cells. ..
Sato A, Yamamoto H, Sakane H, Koyama H, Kikuchi A. Wnt5a regulates distinct signalling pathways by binding to Frizzled2. EMBO J. 2010;29:41-54 pubmed publisher
..These results suggest that Wnt5a regulates distinct pathways through receptor internalization-dependent and -independent mechanisms. ..
Fujimoto N, Kitamura S, Kanno J. Androgen dependent transcription of a mouse prostatic protein gene, PSP94: involvement of estrogen receptors. J Steroid Biochem Mol Biol. 2011;127:301-6 pubmed publisher
..Collectively, these data suggest that the androgen-dependent transcription of the mPSP94 gene was co-regulated/modulated by the presence of ER? via ERE-like motifs. ..
Araki Y, Hamafuji T, Noguchi C, Shimizu N. Efficient recombinant production in mammalian cells using a novel IR/MAR gene amplification method. PLoS ONE. 2012;7:e41787 pubmed publisher
..In conclusion, the IR/MAR gene amplification method is a novel and efficient platform for recombinant antibody production in mammalian cells, which rapidly and easily enables the establishment of stable high-producer cell clone. ..
Tange S, Fujimoto N, Uramaru N, Wong F, Sugihara K, Ohta S, et al
. In vitro metabolism of methiocarb and carbaryl in rats, and its effect on their estrogenic and antiandrogenic activities. Environ Toxicol Pharmacol. 2016;41:289-97 pubmed publisher
..Thus, hydrolysis of methiocarb and carbaryl and oxidation of methiocarb to the sulfoxide markedly modified the estrogenic and antiandrogenic activities of methiocarb and carbaryl. ..
Fukuma M, Ganmyo Y, Miura O, Ohyama T, Shimizu N. Cloning and Characterization of a Human Genomic Sequence that Alleviates Repeat-Induced Gene Silencing. PLoS ONE. 2016;11:e0153338 pubmed publisher
..Because B-3-31 effectively and reproducibly alleviated RIGS of repeated genes, it could be used to increase recombinant protein production. ..
Yamada T, Yamamoto K, Ishihara T, Ohta S. Purification of monoclonal antibodies entirely in flow-through mode. J Chromatogr B Analyt Technol Biomed Life Sci. 2017;1061-1062:110-116 pubmed publisher
..These results represent the possibility of an antibody manufacturing process using an entirely flow-through mode. ..
Fumoto K, Hoogenraad C, Kikuchi A. GSK-3beta-regulated interaction of BICD with dynein is involved in microtubule anchorage at centrosome. EMBO J. 2006;25:5670-82 pubmed
..These results imply that GSK-3beta may function in transporting centrosomal proteins to the centrosome by stabilizing the BICD1 and dynein complex, resulting in the regulation of a focused microtubule organization. ..
Okada N, Shimizu N. Dissection of the beta-globin replication-initiation region reveals specific requirements for replicator elements during gene amplification. PLoS ONE. 2013;8:e77350 pubmed publisher
..The differential sequence requirement might be a consequence of extrachromosomal replication. ..