Experts and Doctors on coenzyme a in Tokyo, Japan
Locale: Tokyo, Japan
Topic: coenzyme a
Luo J, Fukuda E, Takase H, Fushinobu S, Shoun H, Wakagi T. Identification of the lysine residue responsible for coenzyme A binding in the heterodimeric 2-oxoacid:ferredoxin oxidoreductase from Sulfolobus tokodaii, a thermoacidophilic archaeon, using 4-fluoro-7-nitrobenzofurazan as an affinity label. Biochim Biophys Acta. 2009;1794:335-40 pubmed publisher
..K125A showed a large increase in the K(m) value for CoA and showed poor inactivation by NBD-F, compared with K173A and wild type StOFOR, indicating Lys125 in subunit-b is the critical residue that interacts with CoA. ..
Tokutake Y, Onizawa N, Katoh H, Toyoda A, Chohnan S. Coenzyme A and its thioester pools in fasted and fed rat tissues. Biochem Biophys Res Commun. 2010;402:158-62 pubmed publisher
..Thus, a comprehensive analysis of thirteen types of rat tissue revealed that CoA pools have different sizes and showed a different response to fasting and feeding depending on the tissue. ..
Hiyama T, Zhao M, Kitago Y, Yao M, Sekine S, Terada T, et al
. Structural basis of CoA recognition by the Pyrococcus single-domain CoA-binding proteins. J Struct Funct Genomics. 2006;7:119-29 pubmed
..The CoA interaction of the single-domain CoA-binding proteins is significantly different and much more extensive than that of the multi-subunit/multi-domain CoA-binding protein succinyl-CoA synthetase. ..
Hishikawa D, Shindou H, Kobayashi S, Nakanishi H, Taguchi R, Shimizu T. Discovery of a lysophospholipid acyltransferase family essential for membrane asymmetry and diversity. Proc Natl Acad Sci U S A. 2008;105:2830-5 pubmed publisher
..Our findings constitute a critical milestone for our understanding about how membrane diversity and asymmetry are established and their biological significance. ..
Kurihara M, Ohmuro Matsuyama Y, Ayabe K, Yamashita T, Yamaji H, Ueda H. Ultra sensitive firefly luciferase-based protein-protein interaction assay (FlimPIA) attained by hinge region engineering and optimized reaction conditions. Biotechnol J. 2016;11:91-9 pubmed publisher
..As a result, a significantly improved maximal S/B ratio from 2.5 to âˆ¼40 was attained, which promises wider use of the assay in in vitro diagnostics, drug discovery, and expanding our knowledge of various biological phenomena. ..