Experts and Doctors on coenzyme a ligases in Nagoya, Aichi, Japan
Locale: Nagoya, Aichi, Japan
Topic: coenzyme a ligases
Oba Y, Tanaka K, Inouye S. Catalytic properties of domain-exchanged chimeric proteins between firefly luciferase and Drosophila fatty Acyl-CoA synthetase CG6178. Biosci Biotechnol Biochem. 2006;70:2739-44 pubmed
..These results suggest that the N-terminal domain of firefly luciferase is essential for substrate recognition, and that the C-terminal domain is indispensable but not specialized for the luminescence reaction. ..
Oba Y, Iida K, Inouye S. Functional conversion of fatty acyl-CoA synthetase to firefly luciferase by site-directed mutagenesis: a key substitution responsible for luminescence activity. FEBS Lett. 2009;583:2004-8 pubmed publisher
Oshiro T, Aiba H, Mizuno T. A defect in a fatty acyl-CoA synthetase gene, lcf1+, results in a decrease in viability after entry into the stationary phase in fission yeast. Mol Genet Genomics. 2003;269:437-42 pubmed
..pombe is discussed. ..
Oba Y, Ojika M, Inouye S. Characterization of CG6178 gene product with high sequence similarity to firefly luciferase in Drosophila melanogaster. Gene. 2004;329:137-45 pubmed
..Further, phylogenic analyses strongly suggest that the firefly luciferase gene may have evolved from a fatty acyl-CoA synthetase gene as a common ancestral gene. ..
Oba Y, Sato M, Ojika M, Inouye S. Enzymatic and genetic characterization of firefly luciferase and Drosophila CG6178 as a fatty acyl-CoA synthetase. Biosci Biotechnol Biochem. 2005;69:819-28 pubmed
..These results suggest that insects have a novel type of fatty acyl-CoA synthetase. ..
Oba Y, Yoshida M, Shintani T, Furuhashi M, Inouye S. Firefly luciferase genes from the subfamilies Psilocladinae and Ototretinae (Lampyridae, Coleoptera). Comp Biochem Physiol B Biochem Mol Biol. 2012;161:110-6 pubmed publisher
..These results suggest that the primary structures and enzymatic properties of luciferases are conserved in Lampyridae, but the luminescence colors were red-shifted in nocturnal species compared to diurnal species...
Tatsuki M, Mori H. Phosphorylation of tomato 1-aminocyclopropane-1-carboxylic acid synthase, LE-ACS2, at the C-terminal region. J Biol Chem. 2001;276:28051-7 pubmed
..LE-ACS4, however, was not phosphorylated in vitro. These results suggest that ACS isozymes have different post-translational regulatory mechanisms, such as phosphorylation. ..