Experts and Doctors on saccharomyces cerevisiae proteins in Milano, Lombardy, Italy

Summary

Locale: Milano, Lombardy, Italy
Topic: saccharomyces cerevisiae proteins

Top Publications

  1. Ragni E, Fontaine T, Gissi C, Latge J, Popolo L. The Gas family of proteins of Saccharomyces cerevisiae: characterization and evolutionary analysis. Yeast. 2007;24:297-308 pubmed
    ..On the basis of this net distinction, we speculate that the type of C-tail region imposed constraints to the evolution of the N-terminal portion. ..
  2. Giannattasio M, Lazzaro F, Plevani P, Muzi Falconi M. The DNA damage checkpoint response requires histone H2B ubiquitination by Rad6-Bre1 and H3 methylation by Dot1. J Biol Chem. 2005;280:9879-86 pubmed
    ..Such results suggest that histone modifications may have a role in checkpoint function by modulating the interactions of Rad9 with chromatin and active Mec1 kinase. ..
  3. Busti S, Gotti L, Balestrieri C, Querin L, Drovandi G, Felici G, et al. Overexpression of Far1, a cyclin-dependent kinase inhibitor, induces a large transcriptional reprogramming in which RNA synthesis senses Far1 in a Sfp1-mediated way. Biotechnol Adv. 2012;30:185-201 pubmed publisher
  4. Sanvito F, Piatti S, Villa A, Bossi M, Lucchini G, Marchisio P, et al. The beta4 integrin interactor p27(BBP/eIF6) is an essential nuclear matrix protein involved in 60S ribosomal subunit assembly. J Cell Biol. 1999;144:823-37 pubmed
    ..A further function related to the beta4 integrin subunit may have evolved specifically in higher eukaryotic cells. ..
  5. Amara F, Colombo R, Cazzaniga P, Pescini D, Csikasz Nagy A, Falconi M, et al. In vivo and in silico analysis of PCNA ubiquitylation in the activation of the Post Replication Repair pathway in S. cerevisiae. BMC Syst Biol. 2013;7:24 pubmed publisher
    ..Our findings highlighted an intricate functional crosstalk between PRR and other events controlling genome stability, and evidenced that PRR is more complicated and still far less characterized than previously thought. ..
  6. Pessina S, Tsiarentsyeva V, Busnelli S, Vanoni M, Alberghina L, Coccetti P. Snf1/AMPK promotes S-phase entrance by controlling CLB5 transcription in budding yeast. Cell Cycle. 2010;9:2189-200 pubmed
  7. Cotta Ramusino C, Fachinetti D, Lucca C, Doksani Y, Lopes M, Sogo J, et al. Exo1 processes stalled replication forks and counteracts fork reversal in checkpoint-defective cells. Mol Cell. 2005;17:153-9 pubmed
    ..We suggest that Exo1 counteracts fork reversal by resecting newly synthesized chains and resolving the sister chromatid junctions that cause regression of collapsed forks. ..
  8. Anbalagan S, Bonetti D, Lucchini G, Longhese M. Rif1 supports the function of the CST complex in yeast telomere capping. PLoS Genet. 2011;7:e1002024 pubmed publisher
    ..Thus, these data highlight a novel role for Rif1 in assisting the essential telomere protection function of the CST complex. ..
  9. Pescini D, Cazzaniga P, Besozzi D, Mauri G, Amigoni L, Colombo S, et al. Simulation of the Ras/cAMP/PKA pathway in budding yeast highlights the establishment of stable oscillatory states. Biotechnol Adv. 2012;30:99-107 pubmed publisher
    ..In addition, we highlight that the levels of guanine nucleotides pools are able to regulate the pathway, by influencing the transition between stable steady states and oscillatory regimes. ..

More Information

Publications62

  1. Fraschini R, D Ambrosio C, Venturetti M, Lucchini G, Piatti S. Disappearance of the budding yeast Bub2-Bfa1 complex from the mother-bound spindle pole contributes to mitotic exit. J Cell Biol. 2006;172:335-46 pubmed
    ..Moreover, it correlates with the passage of one spindle pole through the bud neck because it needs septin ring formation and bud neck kinases. ..
  2. Coccetti P, Zinzalla V, Tedeschi G, Russo G, Fantinato S, Marin O, et al. Sic1 is phosphorylated by CK2 on Ser201 in budding yeast cells. Biochem Biophys Res Commun. 2006;346:786-93 pubmed
    ..These data strongly support the notion that CK2 phosphorylates Sic1 in vivo. ..
  3. Tiranti V, Galimberti C, Nijtmans L, Bovolenta S, Perini M, Zeviani M. Characterization of SURF-1 expression and Surf-1p function in normal and disease conditions. Hum Mol Genet. 1999;8:2533-40 pubmed
    ..However, detection of residual amounts of fully assembled complex suggests a certain degree of redundancy of this system. ..
  4. Ragni E, Coluccio A, Rolli E, Rodriguez Peña J, Colasante G, Arroyo J, et al. GAS2 and GAS4, a pair of developmentally regulated genes required for spore wall assembly in Saccharomyces cerevisiae. Eukaryot Cell. 2007;6:302-16 pubmed
    ..The ectopic expression of GAS2 and GAS4 genes in a gas1 null mutant revealed that these proteins are redundant versions of Gas1p specialized to function in a compartment at a pH value close to neutral. ..
  5. Paciotti V, Lucchini G, Plevani P, Longhese M. Mec1p is essential for phosphorylation of the yeast DNA damage checkpoint protein Ddc1p, which physically interacts with Mec3p. EMBO J. 1998;17:4199-209 pubmed
    ..We suggest that Rad9p and Ddc1p might function in separated branches of the DNA damage checkpoint pathway, playing different roles in determining Mec1p activity and/or substrate specificity. ..
  6. Clerici M, Mantiero D, Lucchini G, Longhese M. The Saccharomyces cerevisiae Sae2 protein promotes resection and bridging of double strand break ends. J Biol Chem. 2005;280:38631-8 pubmed
    ..Finally, SAE2 deletion slows down resection of an HO-induced DSB and impairs DSB end bridging. Thus, Sae2 participates in DSB single strand annealing repair by ensuring both resection and intrachromosomal association of the broken ends. ..
  7. Bonetti D, Martina M, Clerici M, Lucchini G, Longhese M. Multiple pathways regulate 3' overhang generation at S. cerevisiae telomeres. Mol Cell. 2009;35:70-81 pubmed publisher
  8. Colombo S, Ronchetti D, Thevelein J, Winderickx J, Martegani E. Activation state of the Ras2 protein and glucose-induced signaling in Saccharomyces cerevisiae. J Biol Chem. 2004;279:46715-22 pubmed
    ..Strains with reduced feedback inhibition on cAMP synthesis also display elevated basal and induced Ras2 GTP loading consistent with the Ras2 protein acting as a target of the feedback-inhibition mechanism. ..
  9. Longhese M, Paciotti V, Fraschini R, Zaccarini R, Plevani P, Lucchini G. The novel DNA damage checkpoint protein ddc1p is phosphorylated periodically during the cell cycle and in response to DNA damage in budding yeast. EMBO J. 1997;16:5216-26 pubmed
    ..Moreover, Ddc1p is phosphorylated periodically during a normal cell cycle and becomes hyperphosphorylated in response to DNA damage. Both phosphorylation events are at least partially dependent on a functional MEC3 gene. ..
  10. Bonetti D, Clerici M, Anbalagan S, Martina M, Lucchini G, Longhese M. Shelterin-like proteins and Yku inhibit nucleolytic processing of Saccharomyces cerevisiae telomeres. PLoS Genet. 2010;6:e1000966 pubmed publisher
    ..Thus, chromosome end degradation is controlled by telomeric proteins that specifically inhibit the action of different nucleases. ..
  11. Pearson M, Pelicci P. PML interaction with p53 and its role in apoptosis and replicative senescence. Oncogene. 2001;20:7250-6 pubmed
    ..This review discusses recent data, demonstrating that the Promyelocytic Leukemia (PML) protein can physically and functionally interact with both p53 and Rb, suggesting that PML may be a novel regulator of these pathways. ..
  12. Ferrari M, Nachimuthu B, Donnianni R, Klein H, Pellicioli A. Tid1/Rdh54 translocase is phosphorylated through a Mec1- and Rad53-dependent manner in the presence of DSB lesions in budding yeast. DNA Repair (Amst). 2013;12:347-55 pubmed publisher
    ..In summary, Tid1 is a novel target of the DNA damage checkpoint pathway that is also involved in checkpoint adaptation. ..
  13. Donnianni R, Ferrari M, Lazzaro F, Clerici M, Tamilselvan Nachimuthu B, Plevani P, et al. Elevated levels of the polo kinase Cdc5 override the Mec1/ATR checkpoint in budding yeast by acting at different steps of the signaling pathway. PLoS Genet. 2010;6:e1000763 pubmed publisher
    ..We propose that Cdc5 interferes with the checkpoint response to DSBs acting at multiple levels in the signal transduction pathway and at an early step required to resect DSB ends. ..
  14. Rolli E, Ragni E, Calderon J, Porello S, Fascio U, Popolo L. Immobilization of the glycosylphosphatidylinositol-anchored Gas1 protein into the chitin ring and septum is required for proper morphogenesis in yeast. Mol Biol Cell. 2009;20:4856-70 pubmed publisher
    ..We present evidence that Crh1p and Crh2p are required for tethering Gas1p to the chitin ring and bud scar. These results reveal a new mechanism of protein immobilization at specific sites of the cell envelope. ..
  15. Rossio V, Galati E, Ferrari M, Pellicioli A, Sutani T, Shirahige K, et al. The RSC chromatin-remodeling complex influences mitotic exit and adaptation to the spindle assembly checkpoint by controlling the Cdc14 phosphatase. J Cell Biol. 2010;191:981-97 pubmed publisher
    ..Our data suggest that fine-tuning regulators of mitotic exit have important functions during mitotic progression in cells treated with microtubule poisons and might be promising targets for cancer treatment. ..
  16. Bonetti D, Clerici M, Manfrini N, Lucchini G, Longhese M. The MRX complex plays multiple functions in resection of Yku- and Rif2-protected DNA ends. PLoS ONE. 2010;5:e14142 pubmed publisher
    ..Thus, resection of DNA ends that are protected by Yku and Rif2 involves multiple functions of the MRX complex that do not necessarily require its nuclease activity. ..
  17. Raspelli E, Cassani C, Lucchini G, Fraschini R. Budding yeast Dma1 and Dma2 participate in regulation of Swe1 levels and localization. Mol Biol Cell. 2011;22:2185-97 pubmed publisher
    ..Taken together, the data highlight a previously unknown role of these proteins in the complex regulation of Swe1 and suggest that they might contribute to control, directly or indirectly, Swe1 ubiquitylation. ..
  18. Adamo G, Brocca S, Passolunghi S, Salvato B, Lotti M. Laboratory evolution of copper tolerant yeast strains. Microb Cell Fact. 2012;11:1 pubmed publisher
    ..However, copper elicits different physiological and molecular reactions in yeasts with different backgrounds. ..
  19. Visintin C, Tomson B, Rahal R, Paulson J, Cohen M, Taunton J, et al. APC/C-Cdh1-mediated degradation of the Polo kinase Cdc5 promotes the return of Cdc14 into the nucleolus. Genes Dev. 2008;22:79-90 pubmed publisher
  20. Bonetti D, Anbalagan S, Lucchini G, Clerici M, Longhese M. Tbf1 and Vid22 promote resection and non-homologous end joining of DNA double-strand break ends. EMBO J. 2013;32:275-89 pubmed publisher
    ..Finally, inactivation of either Tbf1 or Vid22 impairs nucleosome eviction around the DSB, suggesting that these proteins promote efficient repair of the break by influencing chromatin identity in its surroundings. ..
  21. Querin L, Sanvito R, Magni F, Busti S, Van Dorsselaer A, Alberghina L, et al. Proteomic analysis of a nutritional shift-up in Saccharomyces cerevisiae identifies Gvp36 as a BAR-containing protein involved in vesicular traffic and nutritional adaptation. J Biol Chem. 2008;283:4730-43 pubmed
    ..We thus propose that Gvp36 represents a novel yeast BAR protein involved in vesicular traffic and in nutritional adaptation. ..
  22. Biffo S, Sanvito F, Costa S, Preve L, Pignatelli R, Spinardi L, et al. Isolation of a novel beta4 integrin-binding protein (p27(BBP)) highly expressed in epithelial cells. J Biol Chem. 1997;272:30314-21 pubmed
    ..These results suggest that the p27(BBP) is an in vivo interactor of beta4, possibly linking beta4 to the intermediate filament cytoskeleton. ..
  23. Chiroli E, Rossio V, Lucchini G, Piatti S. The budding yeast PP2ACdc55 protein phosphatase prevents the onset of anaphase in response to morphogenetic defects. J Cell Biol. 2007;177:599-611 pubmed
    ..We find that the protein phosphatase PP2A bound to its regulatory subunit Cdc55 plays a key role in this process, uncovering a new function for PP2A(Cdc55) in controlling a noncanonical pathway of chromatid cohesion removal. ..
  24. Nespoli A, Vercillo R, di Nola L, Diani L, Giannattasio M, Plevani P, et al. Alk1 and Alk2 are two new cell cycle-regulated haspin-like proteins in budding yeast. Cell Cycle. 2006;5:1464-71 pubmed
    ..Overexpression of ALK2, but not of ALK1, causes a mitotic arrest, which is correlated to the kinase activity of the protein. This finding, together with its cell cycle regulation, suggests a role for Alk2 in the control of mitosis. ..
  25. Giannattasio M, Sabbioneda S, Minuzzo M, Plevani P, Muzi Falconi M. Correlation between checkpoint activation and in vivo assembly of the yeast checkpoint complex Rad17-Mec3-Ddc1. J Biol Chem. 2003;278:22303-8 pubmed
  26. Fraschini R, Formenti E, Lucchini G, Piatti S. Budding yeast Bub2 is localized at spindle pole bodies and activates the mitotic checkpoint via a different pathway from Mad2. J Cell Biol. 1999;145:979-91 pubmed
    ..Altogether, our data suggest that activation of the mitotic checkpoint blocks progression through mitosis by independent and partially redundant mechanisms. ..
  27. Buscemi G, Saracino F, Masnada D, Carbone M. The Saccharomyces cerevisiae SDA1 gene is required for actin cytoskeleton organization and cell cycle progression. J Cell Sci. 2000;113 ( Pt 7):1199-211 pubmed
    ..Altogether our results suggest that disruption of F-actin structure can block cell cycle progression in G(1) and that Sda1 is involved in the control of the actin cytoskeleton. ..
  28. Chiroli E, Fraschini R, Beretta A, Tonelli M, Lucchini G, Piatti S. Budding yeast PAK kinases regulate mitotic exit by two different mechanisms. J Cell Biol. 2003;160:857-74 pubmed
    ..In addition, inhibition of PAK kinases by Cla4t prevents mitotic exit also by a Swe1-independent mechanism impinging directly on the MEN activator Tem1. ..
  29. Orlandi I, Bettiga M, Alberghina L, Vai M. Transcriptional profiling of ubp10 null mutant reveals altered subtelomeric gene expression and insurgence of oxidative stress response. J Biol Chem. 2004;279:6414-25 pubmed
    ..These results argue in favor of an involvement of deubiquitination in transcriptional control and suggest a linkage between oxidative stress and apoptotic pathway in budding yeast. ..
  30. Puddu F, Piergiovanni G, Plevani P, Muzi Falconi M. Sensing of replication stress and Mec1 activation act through two independent pathways involving the 9-1-1 complex and DNA polymerase ?. PLoS Genet. 2011;7:e1002022 pubmed publisher
  31. Lottersberger F, Rubert F, Baldo V, Lucchini G, Longhese M. Functions of Saccharomyces cerevisiae 14-3-3 proteins in response to DNA damage and to DNA replication stress. Genetics. 2003;165:1717-32 pubmed
    ..Finally, the bmh1-169 bmh2Delta and bmh1-170 bmh2Delta mutants show increased rates of spontaneous gross chromosomal rearrangements, indicating that Bmh proteins are required to suppress genome instability. ..
  32. Giannattasio M, Lazzaro F, Longhese M, Plevani P, Muzi Falconi M. Physical and functional interactions between nucleotide excision repair and DNA damage checkpoint. EMBO J. 2004;23:429-38 pubmed
    ..Conversely, elimination of transcription-coupled or global genome repair alone does not affect checkpoints, suggesting a possible interpretation for the heterogeneity in cancer susceptibility observed in different NER syndrome patients. ..
  33. Rolli E, Ragni E, Rodriguez Peña J, Arroyo J, Popolo L. GAS3, a developmentally regulated gene, encodes a highly mannosylated and inactive protein of the Gas family of Saccharomyces cerevisiae. Yeast. 2010;27:597-610 pubmed publisher
  34. Coccetti P, Rossi R, Sternieri F, Porro D, Russo G, Di Fonzo A, et al. Mutations of the CK2 phosphorylation site of Sic1 affect cell size and S-Cdk kinase activity in Saccharomyces cerevisiae. Mol Microbiol. 2004;51:447-60 pubmed
    ..A strong reduction of the same parameters is observed in cells harbouring Sic1S201E. The deregulated coordination between cell size and cell cycle is also apparent at the level of S-Cdk activity. ..
  35. Carotti C, Ragni E, Palomares O, Fontaine T, Tedeschi G, Rodriguez R, et al. Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding. Eur J Biochem. 2004;271:3635-45 pubmed
    ..cerevisiae. The Gas1-C74S protein was totally unable to complement the phenotype of the gas1 null mutant. We found that C74 is an essential residue for the proper folding and maturation of Gas1p. ..
  36. Diani L, Colombelli C, Nachimuthu B, Donnianni R, Plevani P, Muzi Falconi M, et al. Saccharomyces CDK1 phosphorylates Rad53 kinase in metaphase, influencing cellular morphogenesis. J Biol Chem. 2009;284:32627-34 pubmed publisher
    ..Moreover, the Ser-to-Asp mutation, mimicking a constitutive phosphorylation state at site 774, causes sensitivity to calcofluor, supporting a functional linkage between Rad53 and cellular morphogenesis. ..
  37. Barberis M, De Gioia L, Ruzzene M, Sarno S, Coccetti P, Fantucci P, et al. The yeast cyclin-dependent kinase inhibitor Sic1 and mammalian p27Kip1 are functional homologues with a structurally conserved inhibitory domain. Biochem J. 2005;387:639-47 pubmed
    ..Taken together, these findings strongly indicate that budding yeast Sic1 and mammalian p27(Kip1) are functional homologues with a structurally conserved inhibitory domain. ..
  38. Sabbioneda S, Minesinger B, Giannattasio M, Plevani P, Muzi Falconi M, Jinks Robertson S. The 9-1-1 checkpoint clamp physically interacts with polzeta and is partially required for spontaneous polzeta-dependent mutagenesis in Saccharomyces cerevisiae. J Biol Chem. 2005;280:38657-65 pubmed
    ..These results suggest that, in addition to its checkpoint signaling role, the 9-1-1 clamp may physically regulate Polzeta-dependent mutagenesis by controlling the access of Polzeta to damaged DNA. ..
  39. Carotti C, Ferrario L, Roncero C, Valdivieso M, Duran A, Popolo L. Maintenance of cell integrity in the gas1 mutant of Saccharomyces cerevisiae requires the Chs3p-targeting and activation pathway and involves an unusual Chs3p localization. Yeast. 2002;19:1113-24 pubmed
    ..Control of the position of chitin synthesis could be important in protecting the bud from lysis. ..
  40. Trovesi C, Falcettoni M, Lucchini G, Clerici M, Longhese M. Distinct Cdk1 requirements during single-strand annealing, noncrossover, and crossover recombination. PLoS Genet. 2011;7:e1002263 pubmed publisher
    ..Thus, Cdk1 promotes DSB repair by single-strand annealing and noncrossover recombination by acting mostly at the resection level, whereas additional events require Cdk1-dependent regulation in order to generate crossover outcomes. ..
  41. Fraschini R, Beretta A, Sironi L, Musacchio A, Lucchini G, Piatti S. Bub3 interaction with Mad2, Mad3 and Cdc20 is mediated by WD40 repeats and does not require intact kinetochores. EMBO J. 2001;20:6648-59 pubmed
    ..We suggest that Bub3 could serve as a platform for interactions between kinetochore checkpoint proteins, and its association with Mad2, Mad3 and Cdc20 might be instrumental for checkpoint activation. ..
  42. Lazzaro F, Novarina D, Amara F, Watt D, Stone J, Costanzo V, et al. RNase H and postreplication repair protect cells from ribonucleotides incorporated in DNA. Mol Cell. 2012;45:99-110 pubmed publisher
  43. Neecke H, Lucchini G, Longhese M. Cell cycle progression in the presence of irreparable DNA damage is controlled by a Mec1- and Rad53-dependent checkpoint in budding yeast. EMBO J. 1999;18:4485-97 pubmed
  44. Tripodi F, Zinzalla V, Vanoni M, Alberghina L, Coccetti P. In CK2 inactivated cells the cyclin dependent kinase inhibitor Sic1 is involved in cell-cycle arrest before the onset of S phase. Biochem Biophys Res Commun. 2007;359:921-7 pubmed
    ..These findings indicate that Sic1 plays a major role during G1 arrest of CK2-inactivated cells. ..
  45. Fasoli E, Aldini G, Regazzoni L, Kravchuk A, Citterio A, Righetti P. Les Maîtres de l'Orge: the proteome content of your beer mug. J Proteome Res. 2010;9:5262-9 pubmed publisher
    ..The knowledge of the residual proteome in beers might help brewers in selecting proper proteinaceous components that might enrich beer flavor and texture. ..
  46. Bertoli G, Simmen T, Anelli T, Molteni S, Fesce R, Sitia R. Two conserved cysteine triads in human Ero1alpha cooperate for efficient disulfide bond formation in the endoplasmic reticulum. J Biol Chem. 2004;279:30047-52 pubmed
    ..These findings suggest that interactions between the two triads, dependent on Cys85 and Cys391, are important for Ero1alpha function, possibly stabilizing a platform for efficient PDI oxidation. ..
  47. Gualtieri T, Ragni E, Mizzi L, Fascio U, Popolo L. The cell wall sensor Wsc1p is involved in reorganization of actin cytoskeleton in response to hypo-osmotic shock in Saccharomyces cerevisiae. Yeast. 2004;21:1107-20 pubmed
    ..Wsc1p appears to be an important regulator of the actin network rearrangements in conditions of cell wall expansion and membrane stretching. ..
  48. Cipollina C, van den Brink J, Daran Lapujade P, Pronk J, Porro D, de Winde J. Saccharomyces cerevisiae SFP1: at the crossroads of central metabolism and ribosome biogenesis. Microbiology. 2008;154:1686-99 pubmed publisher
    ..Finally, our data suggest a role for Sfp1 in the regulation of glycolysis, further underlining its involvement in the network that links ribosome biogenesis and cell metabolism. ..
  49. Lazzaro F, Sapountzi V, Granata M, Pellicioli A, Vaze M, Haber J, et al. Histone methyltransferase Dot1 and Rad9 inhibit single-stranded DNA accumulation at DSBs and uncapped telomeres. EMBO J. 2008;27:1502-12 pubmed publisher
  50. Coccetti P, Tripodi F, Tedeschi G, Nonnis S, Marin O, Fantinato S, et al. The CK2 phosphorylation of catalytic domain of Cdc34 modulates its activity at the G1 to S transition in Saccharomyces cerevisiae. Cell Cycle. 2008;7:1391-401 pubmed
  51. Cipollina C, van den Brink J, Daran Lapujade P, Pronk J, Vai M, de Winde J. Revisiting the role of yeast Sfp1 in ribosome biogenesis and cell size control: a chemostat study. Microbiology. 2008;154:337-46 pubmed publisher
    ..Our results suggest that Sfp1 plays a role in transcriptional and cell size control, operating at two different levels of the regulatory network linking growth, metabolism and cell size. ..
  52. Merlini L, Fraschini R, Boettcher B, Barral Y, Lucchini G, Piatti S. Budding yeast dma proteins control septin dynamics and the spindle position checkpoint by promoting the recruitment of the Elm1 kinase to the bud neck. PLoS Genet. 2012;8:e1002670 pubmed publisher
    ..Altogether, our data indicate that septin dynamics and SPOC function are intimately linked and support the idea that integrity of the bud neck is crucial for SPOC signalling. ..
  53. Panigada D, Grianti P, Nespoli A, Rotondo G, Castro D, Quadri R, et al. Yeast haspin kinase regulates polarity cues necessary for mitotic spindle positioning and is required to tolerate mitotic arrest. Dev Cell. 2013;26:483-95 pubmed publisher
    ..The evolutionary conservation of haspin and of the polarization mechanisms suggests that this function of haspin is likely shared with other eukaryotes, in which haspin may regulate asymmetric cell division. ..