Experts and Doctors on cattle in Kalyani, West Bengal, India
Locale: Kalyani, West Bengal, India
Chakraborti S, Mandal A, Das S, Chakraborti T. Inhibition of Na+/Ca2+ exchanger by peroxynitrite in microsomes of pulmonary smooth muscle: role of matrix metalloproteinase-2. Biochim Biophys Acta. 2004;1671:70-8 pubmed
Mandal A, Chakraborti T, Choudhury R, Ghosh B, Ghosh A, Das S, et al
. Role of MMP-2 in inhibiting Na+ dependent Ca2+ uptake by H2O2 in microsomes isolated from pulmonary smooth muscle. Mol Cell Biochem. 2005;270:79-87 pubmed
..Thus, one of the mechanisms by which H2O2 activates MMP-2 could be due to inactivation of TIMP-2 by the oxidant. The resulting activation of MMP-2 subsequently inhibits Na+ dependent Ca2+ uptake in the microsomes. ..
Kar P, Chakraborti T, Samanta K, Chakraborti S. Submitochondrial localization of associated mu-calpain and calpastatin. Arch Biochem Biophys. 2008;470:176-86 pubmed
Samanta K, Kar P, Chakraborti T, Chakraborti S. Calcium-dependent cleavage of the Na(+)/Ca(2+) exchanger by m-calpain in isolated endoplasmic reticulum. J Biochem. 2010;147:225-35 pubmed publisher
..Cleavage of NCX1 by m-calpain in the ER may be interpreted as the main cause of intracellular Ca(2+) overload in the smooth muscle, which could be important for the manifestation of pulmonary hypertension. ..
Samanta K, Kar P, Chakraborti T, Shaikh S, Chakraborti S. Characteristic properties of endoplasmic reticulum membrane m-calpain, calpastatin and lumen m-calpain: a comparative study between membrane and lumen m-calpains. J Biochem. 2010;147:765-79 pubmed publisher
..6 and 4.7, respectively) and IC(50) (0.52 and 0.8 nM, respectively) values of 110 and 70 kDa calpastatins. For first time, we have determined the characteristic properties, regulation and functional activity of LCp in the ER lumen. ..
Shaikh S, Samanta K, Kar P, Roy S, Chakraborti T, Chakraborti S. m-Calpain-mediated cleavage of Na+/Ca2+ exchanger-1 in caveolae vesicles isolated from pulmonary artery smooth muscle. Mol Cell Biochem. 2010;341:167-80 pubmed publisher
Mandal M, Das S, Chakraborti T, Mandal A, Chakraborti S. Identification, purification and partial characterization of tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in bovine pulmonary artery smooth muscle. Mol Cell Biochem. 2003;254:145-55 pubmed
..It was also found to be inactive against the serine proteases trypsin and plasmin. The inhibitor was heat and acid resistant and it had the sensitivity to trypsin degradation and reduction-alkylation. ..
Mandal M, Mandal A, Das S, Chakraborti T, Chakraborti S. Identification, purification and partial characterization of tissue inhibitor of matrix metalloproteinase-2 in bovine pulmonary artery smooth muscle. Mol Cell Biochem. 2003;254:275-87 pubmed
..Treatment of the inhibitor with hydrogen peroxide, superoxide generating system (hypoxanthine plus xanthine oxidase) and peroxynitrite inactivated the inhibitor. ..
Das S, Mandal M, Chakraborti T, Mandal A, Chakraborti S. Isolation of MMP-2 from MMP-2/TIMP-2 complex: characterization of the complex and the free enzyme in pulmonary vascular smooth muscle plasma membrane. Biochim Biophys Acta. 2004;1674:158-74 pubmed
..We have also presented additional data indicating that the isolated preparation of 72-kDa gelatinase exhibited properties that are identical with MMP-2 obtained from different sources. ..