Genomes and Genes
Experts and Doctors on amino acid sequence in Hyderabad, Telangana, India
Locale: Hyderabad, Telangana, India
Topic: amino acid sequence
- Kumari G, Mahalingam S. Extracellular signal-regulated kinase 2 (ERK-2) mediated phosphorylation regulates nucleo-cytoplasmic shuttling and cell growth control of Ras-associated tumor suppressor protein, RASSF2. Exp Cell Res. 2009;315:2775-90 pubmed publisher..Taken together, the present study suggests that active transport between nucleus and cytoplasm may constitute an important regulatory mechanism for RASSF2 function. ..
- Jobby M, Sharma Y. Caulollins from Caulobacter crescentus, a pair of partially unstructured proteins of betagamma-crystallin superfamily, gain structure upon binding calcium. Biochemistry. 2007;46:12298-307 pubmed..This work further suggests that Ca2+ binding is a widespread feature of the betagamma-crystallin superfamily. ..
- Sivaramakrishna Y, Amancha P, Siva Kumar N. Reptilian MPR 300 is also the IGF-IIR: cloning, sequencing and functional characterization of the IGF-II binding domain. Int J Biol Macromol. 2009;44:435-40 pubmed publisher..We present here the first report on the calotes MPR 300/IGF-IIR binds IGF-II with K(d) of 12.02 nM; these findings provide new and strong evidence that MPR 300/IGF-IIR in Calotes versicolor binds IGFII with high affinity. ..
- Kumar P, Joshi D, Akif M, Akhter Y, Hasnain S, Mande S. Mapping conformational transitions in cyclic AMP receptor protein: crystal structure and normal-mode analysis of Mycobacterium tuberculosis apo-cAMP receptor protein. Biophys J. 2010;98:305-14 pubmed publisher..The structure of the cAMP-free protein and results of the normal-mode analysis therefore highlight an elegant mechanism of the allosteric changes effected by the binding of cAMP. ..
- Jafurulla M, Tiwari S, Chattopadhyay A. Identification of cholesterol recognition amino acid consensus (CRAC) motif in G-protein coupled receptors. Biochem Biophys Res Commun. 2011;404:569-73 pubmed publisher..These results constitute the first report of the presence of CRAC motifs in GPCRs and provide novel insight in the molecular nature of GPCR-cholesterol interaction. ..
- Bhargavi R, Sastry G, Murty U, Sastry G. Structural and active site analysis of plasmepsins of Plasmodium falciparum: potential anti-malarial targets. Int J Biol Macromol. 2005;37:73-84 pubmed..Large buried side chain area of residues located at M15 and I289 of PMII (and corresponding residues of PMI and PMIV) corroborates well with increase in specificity constant for hydrophobic substrates. ..
- Aparoy P, Leela T, Reddy R, Reddanna P. Computational analysis of R and S isoforms of 12-lipoxygenases: homology modeling and docking studies. J Mol Graph Model. 2009;27:744-50 pubmed publisher..The docking of the substrate, arachidonic acid was also performed. Our results show that the Gly441 and substrate orientation within the active site play an important role in stereo specificity of 12R-LOX. ..
- Vijayan S, Imani J, Tanneeru K, Guruprasad L, Kogel K, Kirti P. Enhanced antifungal and insect ?-amylase inhibitory activities of Alpha-TvD1, a peptide variant of Tephrosia villosa defensin (TvD1) generated through in vitro mutagenesis. Peptides. 2012;33:220-9 pubmed publisher..Present study shows that the variant Alpha-TvD1 exhibits enhanced antifungal as well as insect ?-amylase inhibitory activity...
- Sitaram N, Nagaraj R. Interaction of antimicrobial peptides with biological and model membranes: structural and charge requirements for activity. Biochim Biophys Acta. 1999;1462:29-54 pubmed..In this review, we discuss structural and charge requirements for the interaction of endogenous antimicrobial peptides and short peptides that have been derived from them, with membranes. ..
- Mitra K, Shivaji S. Novel tyrosine-phosphorylated post-pyruvate metabolic enzyme, dihydrolipoamide dehydrogenase, involved in capacitation of hamster spermatozoa. Biol Reprod. 2004;70:887-99 pubmed publisher..The essentiality of pyruvate-lactate during hyperactivation and acrosome reaction necessitates the involvement of the post-pyruvate-lactate enzyme, dihydrolipoamide dehydrogenase...
- Paila U, Kondam R, Ranjan A. Genome bias influences amino acid choices: analysis of amino acid substitution and re-compilation of substitution matrices exclusive to an AT-biased genome. Nucleic Acids Res. 2008;36:6664-75 pubmed publisher..This study has important implications on annotation of proteins that are of experimental interest but give poor sequence alignments with standard conventional matrices. ..
- Tanneeru K, Guruprasad L. Structural basis for binding of aurora-AG198N- INCENP complex: MD simulations and free energy calculations. Protein Pept Lett. 2013;20:1246-56 pubmed..Our results provide the structural basis and energetics of the human Aurora-A(G198N) - INCENP complex. ..
- Asthana A, Raman B, Ramakrishna T, Rao C. Structural aspects and chaperone activity of human HspB3: role of the "C-terminal extension". Cell Biochem Biophys. 2012;64:61-72 pubmed publisher..Our study, for the first time, provides the structural and chaperone functional characterization of HspB3 and also sheds light on the role of the C-terminal extension of sHsps. ..
- Kumar K, Kirti P. A mitogen-activated protein kinase, AhMPK6 from peanut localizes to the nucleus and also induces defense responses upon transient expression in tobacco. Plant Physiol Biochem. 2010;48:481-6 pubmed publisher..The AhMPK6 induced gene expression suggests an overlapping or partially redundant function with previously reported AhMPK3. ..
- Sowmya B, Jagannadham M, Nagaraj R. Interaction of synthetic peptides corresponding to the scaffolding domain of Caveolin-3 with model membranes. Biopolymers. 2006;84:615-24 pubmed..Association with lipids decreases considerably when TFT as well as the aromatic-rich segment YWFYR, which occurs at the extreme C-terminus of the scaffolding domain, are deleted. ..
- Thavathiru E, Jana N, De P. Abundant secretory lipocalins displaying male and lactation-specific expression in adult hamster submandibular gland. cDNA cloning and sex hormone-regulated repression. Eur J Biochem. 1999;266:467-76 pubmed..The results of homology search and the male- and lactation-specific pressure of the SMG proteins in adult saliva and urine suggests a possibility of their involvement in olfaction-mediated chemical communication between hamsters. ..
- Mishra S. Computational prediction of protein-protein complexes. BMC Res Notes. 2012;5:495 pubmed publisher..Consensus generated in their predictions lends credence to the use of the various softwares used. ..
- Goyal A, Verma P, Anandhakrishnan M, Gokhale R, Sankaranarayanan R. Molecular basis of the functional divergence of fatty acyl-AMP ligase biosynthetic enzymes of Mycobacterium tuberculosis. J Mol Biol. 2012;416:221-38 pubmed publisher..The work presented here establishes an elegant mechanism wherein an insertion sequence drives the functional divergence of FAALs from canonical FACLs. ..
- Dixit H, Deendayal M, Singh L. Mutational analysis of the mature peptide region of inhibin genes in Indian women with ovarian failure. Hum Reprod. 2004;19:1760-4 pubmed..001) with complete absence of this mutation in controls (none out of 100). The INHalpha gene is a strong candidate gene for ovarian failure. Mutations in INHbetaB and INHbetaA genes are not associated with ovarian failure. ..
- Rajesh A, Madhubabu G, Yenugu S. Identification and characterization of Wfdc gene expression in the male reproductive tract of the rat. Mol Reprod Dev. 2011;78:633-41 pubmed publisher..For the first time we report the induction of Wfdc8 gene expression in LPS-treated rats, indicating their contributions to the innate immune functions of the male reproductive tract. ..
- Rawat A, Harishchandran A, Nagaraj R. Fatty acyl chain-dependent but charge-independent association of the SH4 domain of Lck with lipid membranes. J Biosci. 2013;38:63-71 pubmed..It has an important role in regulating the spatial orientation of the peptide domain with respect to the lipid bilayer, which could be important for the interaction of the other domains of these kinases with their partners. ..
- Pallavi B, Nagaraj R. Palmitoylated peptides from the cysteine-rich domain of SNAP-23 cause membrane fusion depending on peptide length, position of cysteines, and extent of palmitoylation. J Biol Chem. 2003;278:12737-44 pubmed..Our results suggest an important role for the cysteine-rich palmitoylated domain of SNAP-23 in promoting membrane fusion in cells. ..
- Vindal V, Suma K, Ranjan A. GntR family of regulators in Mycobacterium smegmatis: a sequence and structure based characterization. BMC Genomics. 2007;8:289 pubmed
- Nagarajan T, Nagendrakumar S, Mohanasubramanian B, Rajalakshmi S, Hanumantha N, Ramya R, et al. Phylogenetic analysis of nucleoprotein gene of dog rabies virus isolates from Southern India. Infect Genet Evol. 2009;9:976-82 pubmed publisher..The data indicated that dog rabies virus variants are the major circulating viruses and control of dog rabies would result in overall reduction in the burden and incidence of rabies in India. ..
- Pathak R, Rangaraj N, Kallappagoudar S, Mishra K, Mishra R. Boundary element-associated factor 32B connects chromatin domains to the nuclear matrix. Mol Cell Biol. 2007;27:4796-806 pubmed..These results suggest that boundary elements may function by tethering chromatin to nuclear architectural components and thereby provide a structural basis for compartmentalization of the genome into functionally independent domains...
- Senthilkumar R, Mishra R. Novel motifs distinguish multiple homologues of Polycomb in vertebrates: expansion and diversification of the epigenetic toolkit. BMC Genomics. 2009;10:549 pubmed publisher..The signature motifs of PC homologues and insect specific motif identified in this study pave the way to understand the molecular basis of epigenetic mechanisms. ..
- Koduru S, Vegiraju S, Nadimpalli S, von Figura K, Pohlmann R, Dennes A. The early vertebrate Danio rerio Mr 46000 mannose-6-phosphate receptor: biochemical and functional characterisation. Dev Genes Evol. 2006;216:133-43 pubmed..Interestingly, the serine residue suggested to regulate the interaction between the tail and the GGAs in a phosphorylation-dependent manner is substituted by a proline residue in fish. ..
- Mathew B, Nagaraj R. Variations in the interaction of human defensins with Escherichia coli: Possible implications in bacterial killing. PLoS ONE. 2017;12:e0175858 pubmed publisher..coli is observed. Our data indicate that mammalian defensins do not kill E. coli by a simple mechanism involving membrane permeabilization though their antibacterial potencies are very similar...
- Srinivasan T, Kumar K, Kirti P. Constitutive expression of a trypsin protease inhibitor confers multiple stress tolerance in transgenic tobacco. Plant Cell Physiol. 2009;50:541-53 pubmed publisher..To our knowledge, this is the first report of transgenic plants with constitutive protease inhibitor expression showing tolerance to a wide range variable pH in the culture medium along with other stresses...
- Kannan K, Janiyani K, Shivaji S, Ray M. Histidine utilisation operon (hut) is upregulated at low temperature in the antarctic psychrotrophic bacterium Pseudomonas syringae. FEMS Microbiol Lett. 1998;161:7-14 pubmed..syringae, P. fluorescens and P. putida also suggested that the hut operon is expressed at an elevated level at low temperature...
- Ghosh P, Ramakrishnan C, Chatterji D. Inter-subunit recognition and manifestation of segmental mobility in Escherichia coli RNA polymerase: a case study with omega-beta' interaction. Biophys Chem. 2003;103:223-37 pubmed
- Reddy M, Gowrishankar J. Identification and characterization of ssb and uup mutants with increased frequency of precise excision of transposon Tn10 derivatives: nucleotide sequence of uup in Escherichia coli. J Bacteriol. 1997;179:2892-9 pubmed..The uup gene is also situated immediately upstream of (and is transcribed in the same direction as) the paraquat-inducible SoxRS-regulated pqi-5 gene, two reported promoters for which are situated within the uup coding sequence...
- Divya K, Jami S, Kirti P. Constitutive expression of mustard annexin, AnnBj1 enhances abiotic stress tolerance and fiber quality in cotton under stress. Plant Mol Biol. 2010;73:293-308 pubmed publisher..The transgenic plants maintained normal seed development, fiber quality and cellulose content under stress. ..
- Kumari G, Singhal P, Rao M, Mahalingam S. Nuclear transport of Ras-associated tumor suppressor proteins: different transport receptor binding specificities for arginine-rich nuclear targeting signals. J Mol Biol. 2007;367:1294-311 pubmed..Together, these data suggest that the transport of Ras effector superfamily proteins into the nucleus/nucleolus may play a vital role in modulating Ras-mediated cell proliferation during tumorigenesis. ..
- Mishra A, Suman S, Srivastava S, Sankaranarayanan R, Sharma Y. Decoding the molecular design principles underlying Ca(2+) binding to ??-crystallin motifs. J Mol Biol. 2012;415:75-91 pubmed publisher..Decoding such design schemes would aid in our understanding of the functional themes underlying differential Ca(2)(+) binding and in predicting these in emerging sequence information...
- Rajesh A, Yenugu S. Genomic organization, tissue distribution and functional characterization of the rat Pate gene cluster. PLoS ONE. 2012;7:e32633 pubmed publisher..Pate expression was induced in the epididymides when challenged with LPS. Based on our results, we conclude that rat PATE proteins may contribute to the reproductive and defense functions. ..
- Bimolata W, Kumar A, Sundaram R, Laha G, Qureshi I, Reddy G, et al. Analysis of nucleotide diversity among alleles of the major bacterial blight resistance gene Xa27 in cultivars of rice (Oryza sativa) and its wild relatives. Planta. 2013;238:293-305 pubmed publisher..However, the absence of functional UPT box in all the alleles except IRBB27 suggests the maintenance of single resistant allele throughout the natural population. ..
- Shukla J, Nagaraju J. Doublesex: a conserved downstream gene controlled by diverse upstream regulators. J Genet. 2010;89:341-56 pubmed..This brief review will focus on the similarities and variations of dsx homologues that have been investigated in insects to date. ..
- Marada A, Allu P, Murari A, Pullareddy B, Tammineni P, Thiriveedi V, et al. Mge1, a nucleotide exchange factor of Hsp70, acts as an oxidative sensor to regulate mitochondrial Hsp70 function. Mol Biol Cell. 2013;24:692-703 pubmed publisher..This novel oxidative sensor capability of yeast Mge1 might represent an evolutionarily conserved function, given that human recombinant dimeric Mge1 is also sensitive to H2O2...
- Sulthana S, Rajyaguru P, Mittal P, Ray M. rnr gene from the antarctic bacterium Pseudomonas syringae Lz4W, encoding a psychrophilic RNase R. Appl Environ Microbiol. 2011;77:7896-904 pubmed publisher..This is the first report on the biochemical property of a psychrophilic RNase R from any bacterium...
- Korupolu R, Achary M, Aneesa F, Sathish K, Wasia R, Sairam M, et al. Profilin oligomerization and its effect on poly (L-proline) binding and phosphorylation. Int J Biol Macromol. 2009;45:265-73 pubmed publisher..In silico studies revealed that PKC phosphorylation site, S137 is buried in dimer while it is accessible in tetramer. ..
- Khandelwal R, Sipani R, Govinda Rajan S, Kumar R, Joshi R. Combinatorial action of Grainyhead, Extradenticle and Notch in regulating Hox mediated apoptosis in Drosophila larval CNS. PLoS Genet. 2017;13:e1007043 pubmed publisher..We propose a mechanism where common players like Exd-Grh-Notch work with different Hox genes through region specific enhancers to pattern respective segments of larval central nervous system. ..
- Ghosh P, Ishihama A, Chatterji D. Escherichia coli RNA polymerase subunit omega and its N-terminal domain bind full-length beta' to facilitate incorporation into the alpha2beta subassembly. Eur J Biochem. 2001;268:4621-7 pubmed..It is shown here that omega participates during the last stage of enzyme assembly when beta' associates with the alpha2beta subassembly. ..
- Reddy G, Kumar P, Kumar M. Chaperone-like activity and hydrophobicity of alpha-crystallin. IUBMB Life. 2006;58:632-41 pubmed..We thus attempted to provide an overview on the role of hydrophobicity in chaperone-like activity of alpha-crystallin, the kind of evaluation done for the first time. ..
- Budatha M, Meur G, Dutta Gupta A. A novel aminopeptidase in the fat body of the moth Achaea janata as a receptor for Bacillus thuringiensis Cry toxins and its comparison with midgut aminopeptidase. Biochem J. 2007;405:287-97 pubmed..These data indicate that the fat body might be a potential alternative Cry toxin target site in the moth...
- Jami S, Swathi Anuradha T, Guruprasad L, Kirti P. Molecular, biochemical and structural characterization of osmotin-like protein from black nightshade (Solanum nigrum). J Plant Physiol. 2007;164:238-52 pubmed..Analysis of the structure and functional relationship was carried out by docking of the beta-(1,3)-glucan onto the acidic cleft region on the surface of the protein (SniOLP)...
- Akhter Y, Yellaboina S, Farhana A, Ranjan A, Ahmed N, Hasnain S. Genome scale portrait of cAMP-receptor protein (CRP) regulons in mycobacteria points to their role in pathogenesis. Gene. 2008;407:148-58 pubmed..The conservation of the CRP regulated genes in pathogenic mycobacteria, as opposed to non-pathogenic ones, highlights the importance of CRP-regulated genes in pathogenesis. ..
- Adindla S, Guruprasad K, Guruprasad L. Three-dimensional models and structure analysis of corynemycolyltransferases in Corynebacterium glutamicum and Corynebacterium efficiens. Int J Biol Macromol. 2004;34:181-9 pubmed
- Regha K, Satapathy A, Ray M. RecD plays an essential function during growth at low temperature in the antarctic bacterium Pseudomonas syringae Lz4W. Genetics. 2005;170:1473-84 pubmed..Additional results suggest that a truncated recD encoding the N-terminal segment of (1-576) amino acids is sufficient to support growth of P. syringae at low temperature. ..
- Bharathi V, Pallavi S, Bajpai R, Emerald B, Shashidhara L. Genetic characterization of the Drosophila homologue of coronin. J Cell Sci. 2004;117:1911-22 pubmed..Our results provide a genetic link between endocytosis/exocytosis events involving F actin-coated vesicles and the establishment of morphogen gradient. ..
- Kumar K, Ravi Kumar B, Siddavattam D, Subramanyam C. Characterization of calcineurin-dependent response element binding protein and its involvement in copper-metallothionein gene expression in Neurospora. Biochem Biophys Res Commun. 2006;345:1010-3 pubmed publisher..Parallel identification of nuclear localization signals in this protein by in silico analysis suggests a putative role for calcineurin in the regulation of CuMT gene expression...
- Bashyam M, Purushotham G, Chaudhary A, Rao K, Acharya V, Mohammad T, et al. A low prevalence of MYH7/MYBPC3 mutations among familial hypertrophic cardiomyopathy patients in India. Mol Cell Biochem. 2012;360:373-82 pubmed publisher..The study has revealed a greater frequency of occurrence of MYBPC3 mutations when compared to MYH7 mutations. ..
- Narmadha G, Muneswararao K, Rajesh A, Yenugu S. Characterization of a novel lysozyme-like 4 gene in the rat. PLoS ONE. 2011;6:e27659 pubmed publisher..To the best of our knowledge, for the first time we report the characterization of Lyzl genes in the rat. Results of our study indicate that rat LYZL proteins may have an important role in male reproductive tract function. ..
- Sharma M, Khanna S, Bulusu G, Mitra A. Comparative modeling of thioredoxin glutathione reductase from Schistosoma mansoni: a multifunctional target for antischistosomal therapy. J Mol Graph Model. 2009;27:665-75 pubmed publisher..This also augments recent observations reported on the basis of X-ray structure data on SmTGR monomer lacking the C-terminal selenocysteine tail. ..
- Singh B, Suresh A, UmaPrasad G, Subramanian S, Sultana M, Goel S, et al. A highly conserved human gene encoding a novel member of WD-repeat family of proteins (WDR13). Genomics. 2003;81:315-28 pubmed..The subcellular localization of the WDR13 protein in the nucleus suggests that it may also have a regulatory role in nuclear function along with protein-protein interaction like other members of the WD family of proteins. ..
- Khattri A, Reddy V, Pandey R, Sudhakar D, Gupta N, Chakravarty B, et al. Novel mutations in calcium/calmodulin-dependent protein kinase IV (CAMK4) gene in infertile men. Int J Androl. 2012;35:810-8 pubmed publisher..Our study suggests that the mutations in CAMK4 may lead to abnormal semen parameters. ..
- Prakash A, Kasbekar D. Genes encoding chimeras of Neurospora crassa erg-3 and human TM7SF2 proteins fail to complement Neurospora and yeast sterol C-14 reductase mutants. J Biosci. 2002;27:105-12 pubmed..A few of the chimeric proteins were also tested against the yeast erg24 mutant, but again there was no complementation. We discuss some reasons that might account for these unexpected findings. ..
- Janiyani K, Ray M. Cloning, sequencing, and expression of the cold-inducible hutU gene from the antarctic psychrotrophic bacterium Pseudomonas syringae. Appl Environ Microbiol. 2002;68:1-10 pubmed..The psychrotrophic enzyme, however, has an extended N-terminal end...
- Shivakrupa -, Singh R, Swarup G. Identification of a novel splice variant of C3G which shows tissue-specific expression. DNA Cell Biol. 1999;18:701-8 pubmed..Thus, in rat cells, we have identified a novel splice variant of C3G. The expression pattern indicates that the form of C3G described here is likely to serve a tissue- or cell-specific physiological function. ..
- Chalissery J, Banerjee S, Bandey I, Sen R. Transcription termination defective mutants of Rho: role of different functions of Rho in releasing RNA from the elongation complex. J Mol Biol. 2007;371:855-72 pubmed..The rate of ATP hydrolysis regulates the processivity of translocation along the RNA and is directly correlated with the efficiency of RNA release. NusG improves the speed of RNA release and is not involved in any other step. ..
- Ponnam S, Ramesha K, Tejwani S, Ramamurthy B, Kannabiran C. Mutation of the gap junction protein alpha 8 (GJA8) gene causes autosomal recessive cataract. J Med Genet. 2007;44:e85 pubmed..A single base insertion leading to frameshift at codon 203 of connexin 50 was found to co-segregate with disease in the family. These results confirm involvement of GJA8 in autosomal recessive cataract. ..
- Sebastian J, Ravi M, Andreuzza S, Panoli A, Marimuthu M, Siddiqi I. The plant adherin AtSCC2 is required for embryogenesis and sister-chromatid cohesion during meiosis in Arabidopsis. Plant J. 2009;59:1-13 pubmed publisher..These results demonstrate a role for AtSCC2 in sister-chromatid cohesion and centromere organization, and show that the machinery responsible for the establishment of cohesion is conserved in plants. ..
- Amancha P, Nadimpalli S. Molecular cloning, expression and functional characterization of the chicken cation dependent mannose 6-phosphate receptor protein. Int J Biol Macromol. 2008;43:300-6 pubmed publisher..3%) as compared to mock transfected cells (50.2%), establishing a functional role for the chicken receptor. ..
- Korripally P, Tiwari A, Haritha A, Kiranmayi P, Bhanoori M. Characterization of Ctr family genes and the elucidation of their role in the life cycle of Neurospora crassa. Fungal Genet Biol. 2010;47:237-45 pubmed publisher..Functional analysis of their gene knockout mutants showed that tcu-1 is essential for saprophytic conidial germination, vegetative growth and perithecia development under copper limited conditions while conidiation remained unaffected...
- Mrinal N, Nagaraju J. Intron loss is associated with gain of function in the evolution of the gloverin family of antibacterial genes in Bombyx mori. J Biol Chem. 2008;283:23376-87 pubmed publisher..As a result, the N-terminal pro-part of BmGlv2, but not of BmGlv1, gets processed in an infection-dependent manner suggesting that prepro-processing is an evolved feature in Gloverins...
- Kesanakurti D, Sareddy G, Babu P, Kirti P. Mustard NPR1, a mammalian IkappaB homologue inhibits NF-kappaB activation in human GBM cell lines. Biochem Biophys Res Commun. 2009;390:427-33 pubmed publisher..These results suggested that inhibition of NF-kappaB activation by BjNPR1 can be a promising therapy in NF-kappaB dependent pathologies. ..
- Reddy R, Swarup G. Alternative splicing generates four different forms of a non-transmembrane protein tyrosine phosphatase mRNA. DNA Cell Biol. 1995;14:1007-15 pubmed..Two of these forms, PTP-S1 and PTP-S3, were not found in human cells, possibly due to the loss of an internal splice acceptor site in one of the exons, suggesting the occurrence of species-specific splicing in this gene. ..
- Muralikrishna T, Begum Z, Swamy C, Khar A. Molecular cloning and characterization of a tumor rejection antigen from rat histiocytoma, AK-5. DNA Cell Biol. 1998;17:603-12 pubmed..These observations suggest the recombinant protein to be a strong candidate for the tumor rejection antigen that is involved in the spontaneous regression of the AK-5 tumor in syngeneic hosts. ..
- Chiliveri S, Aute R, Rai U, Deshmukh M. DRB4 dsRBD1 drives dsRNA recognition in Arabidopsis thaliana tasi/siRNA pathway. Nucleic Acids Res. 2017;45:8551-8563 pubmed publisher..Furthermore, we identify a potential role of the C-terminal region of DRB4 in protein:protein interaction as it possesses six PxxP motifs, binds to Zn2+ and contains a small structural domain. ..
- Aravind P, Wistow G, Sharma Y, Sankaranarayanan R. Exploring the limits of sequence and structure in a variant betagamma-crystallin domain of the protein absent in melanoma-1 (AIM1). J Mol Biol. 2008;381:509-18 pubmed publisher..This structure illustrates the resilience of the betagamma fold to considerable sequence changes and its remarkable ability to adapt for novel functions. ..
- Kumar C, Khare G, Srikanth C, Tyagi A, Sardesai A, Mande S. Facilitated oligomerization of mycobacterial GroEL: evidence for phosphorylation-mediated oligomerization. J Bacteriol. 2009;191:6525-38 pubmed publisher..tuberculosis GroEL1 revealed that the tetradecameric form is phosphorylated on serine-393, while the heptameric form is not, indicating that the switch between the single- and double-ring variants is mediated by phosphorylation. ..
- Jagannadham M, Sharadadevi A, Nagaraj R. Effects of deleting a tripeptide sequence observed in muscular dystrophy patients on the conformation of synthetic peptides corresponding to the scaffolding domain of caveolin-3. Biochem Biophys Res Commun. 2002;298:203-6 pubmed..It is likely that the structural changes arising as a result of TFT deletion distrupt oligomerization and consequently mistargeting and degradation. ..
- Kumar K, Kirti P. Novel role for a serine/arginine-rich splicing factor, AdRSZ21 in plant defense and HR-like cell death. Plant Mol Biol. 2012;80:461-76 pubmed publisher..The cell death induced by AdRSZ21 was found to be associated with specific upregulation of patatin-like protein gene and other defense related gene transcripts suggesting a role for AdRSZ21 in plant defense and HR-like cell death. ..
- Rajini B, Graham C, Wistow G, Sharma Y. Stability, homodimerization, and calcium-binding properties of a single, variant betagamma-crystallin domain of the protein absent in melanoma 1 (AIM1). Biochemistry. 2003;42:4552-9 pubmed..These results show that, despite the sequence variation, AIM1-g1 folds such as a betagamma domain, binds calcium and undergoes dimerization. ..
- RamaDevi A, Reddy E, Ranjan S, Bashyam M. Molecular genetic analysis of patients from India with hypohidrotic ectodermal dysplasia reveals novel mutations in the EDA and EDAR genes. Br J Dermatol. 2008;158:163-7 pubmed
- Jobby M, Sharma Y. Calcium-binding to lens betaB2- and betaA3-crystallins suggests that all beta-crystallins are calcium-binding proteins. FEBS J. 2007;274:4135-47 pubmed..Our results have important implications for understanding the calcium-related cataractogenesis and maintenance of ionic homeostasis in the lens. ..
- Savithri B, Khar A. A transmembrane-anchored rat RAE-1-like transcript as a ligand for NKR-P2, the rat ortholog of human and mouse NKG2D. Eur J Immunol. 2006;36:107-17 pubmed..Ectopic expression of the ligand induces NK cell activation, and renders the target cells susceptible to NK cell killing. In addition, the role of RRLT-NKR-P2 interaction in tumor killing has been demonstrated. ..
- Suman S, Mishra A, Ravindra D, Yeramala L, Sharma Y. Evolutionary remodeling of ??-crystallins for domain stability at cost of Ca2+ binding. J Biol Chem. 2011;286:43891-901 pubmed publisher..Thus, this motif acts as a "central tuning knob" for innate as well as Ca2+-induced gain in stability, incorporating a stability gradient across ??-crystallin members critical for their specialized functions...
- Babu P, Rao K, Reddy V. Structural organization and classification of cytochrome P450 genes in flax (Linum usitatissimum L.). Gene. 2013;513:156-62 pubmed publisher..About 59% of the flax CYP450 genes were present in the EST libraries...
- Pasta S, Raman B, Ramakrishna T, Rao C. Role of the C-terminal extensions of alpha-crystallins. Swapping the C-terminal extension of alpha-crystallin to alphaB-crystallin results in enhanced chaperone activity. J Biol Chem. 2002;277:45821-8 pubmed..Our study demonstrates that the unstructured C-terminal extensions play a crucial role in the structure and chaperone activity, in addition to generally believed electrostatic "solubilizer" function. ..