Experts and Doctors on binding sites in Crete, Greece


Locale: Crete, Greece
Topic: binding sites

Top Publications

  1. Androutsopoulos V, Papakyriakou A, Vourloumis D, Spandidos D. Comparative CYP1A1 and CYP1B1 substrate and inhibitor profile of dietary flavonoids. Bioorg Med Chem. 2011;19:2842-9 pubmed publisher
  2. Polioudaki H, Kourmouli N, Drosou V, Bakou A, Theodoropoulos P, Singh P, et al. Histones H3/H4 form a tight complex with the inner nuclear membrane protein LBR and heterochromatin protein 1. EMBO Rep. 2001;2:920-5 pubmed
  3. Spyridaki A, Matzen C, Lanio T, Jeltsch A, Simoncsits A, Athanasiadis A, et al. Structural and biochemical characterization of a new Mg(2+) binding site near Tyr94 in the restriction endonuclease PvuII. J Mol Biol. 2003;331:395-406 pubmed
    ..These results, for the first time, shed light on the pathway by which metal ions as essential cofactors enter the catalytic center of restriction endonucleases...
  4. Zarifi I, Kiparaki M, Koumbanakis K, Giagtzoglou N, Zacharioudaki E, Alexiadis A, et al. Essential roles of Da transactivation domains in neurogenesis and in E(spl)-mediated repression. Mol Cell Biol. 2012;32:4534-48 pubmed publisher
    ..We present a mechanistic model on the interplay of these bHLH factors in the context of neural fate assignment. ..
  5. Gkountelias K, Papadokostaki M, Javitch J, Liapakis G. Exploring the binding site crevice of a family B G protein-coupled receptor, the type 1 corticotropin releasing factor receptor. Mol Pharmacol. 2010;78:785-93 pubmed publisher
    ..Thus, Cys211, Cys233, and Cys364 at the cytoplasmic ends of the third, fourth, and seventh membrane-spanning segments, respectively, are exposed in the binding site crevice of CRF(1). ..
  6. Daskalakis V, Pinakoulaki E, Stavrakis S, Varotsis C. Probing the environment of cu(b) in heme-copper oxidases. J Phys Chem B. 2007;111:10502-9 pubmed
  7. Kouskouti A, Scheer E, Staub A, Tora L, Talianidis I. Gene-specific modulation of TAF10 function by SET9-mediated methylation. Mol Cell. 2004;14:175-82 pubmed
    ..The promoter-specific effects of SET9-methylated TAF10 may have important implications regarding the biological function of SET domain-containing lysine methylases, whose primary targets have been presumed to be histones. ..
  8. Gounalaki N, Tzamarias D, Vlassi M. Identification of residues in the TPR domain of Ssn6 responsible for interaction with the Tup1 protein. FEBS Lett. 2000;473:37-41 pubmed
    ..According to this model all selected mutations reduce the ability of Ssn6 to interact with Tup1 by affecting the structural integrity of TPR1 and/or the correct spatial arrangement of TPR1 relative to TPR2 and TPR3. ..
  9. Baud C, Karamanou S, Sianidis G, Vrontou E, Politou A, Economou A. Allosteric communication between signal peptides and the SecA protein DEAD motor ATPase domain. J Biol Chem. 2002;277:13724-31 pubmed
    ..Our data reveal the physical determinants and sophisticated intramolecular regulation that allow signal peptides to act as allosteric effectors of the SecA motor. ..

More Information


  1. Gagou M, Kapsetaki M, Turberg A, Kafetzopoulos D. Stage-specific expression of the chitin synthase DmeChSA and DmeChSB genes during the onset of Drosophila metamorphosis. Insect Biochem Mol Biol. 2002;32:141-6 pubmed
    ..Transcripts of either gene are barely detected prior to and during the late-larval ecdysone pulse. Once the late-larval ecdysone pulse is ceased completely, both DmeChSA and DmeChSB genes show a remarkable up-regulation. ..
  2. Hatzis P, Talianidis I. Regulatory mechanisms controlling human hepatocyte nuclear factor 4alpha gene expression. Mol Cell Biol. 2001;21:7320-30 pubmed
    ..We present evidence that this sequence is a bona fide retinoic acid response element and that HNF-4alpha expression is upregulated in vivo upon retinoic acid signaling. ..
  3. Voutsina A, Fragiadakis G, Boutla A, Alexandraki D. The second cysteine-rich domain of Mac1p is a potent transactivator that modulates DNA binding efficiency and functionality of the protein. FEBS Lett. 2001;494:38-43 pubmed
    ..The efficiency of binding to both copper-response promoter elements can determine the in vivo functionality of Mac1p independent of homodimerization. ..
  4. Chroni S, Ghanotakis D. Accessibility of tyrosine Y(.)(Z) to exogenous reductants and Mn(2+) in various Photosystem II preparations. Biochim Biophys Acta. 2001;1504:432-7 pubmed
    ..Z) to exogenous reductants, such as benzidine. In addition, it was shown that in the presence of the native Mn complex exogenous Mn(2+) does not reduce Y(.)(Z). ..
  5. Kourmouli N, Theodoropoulos P, Dialynas G, Bakou A, Politou A, Cowell I, et al. Dynamic associations of heterochromatin protein 1 with the nuclear envelope. EMBO J. 2000;19:6558-68 pubmed
    ..A His(6)-tagged peptide representing this region inhibited recruitment of LAP2beta and B-type lamins around the surfaces of condensed chromosomes, suggesting involvement of HP1 proteins in nuclear envelope reassembly. ..
  6. Spilianakis C, Papamatheakis J, Kretsovali A. Acetylation by PCAF enhances CIITA nuclear accumulation and transactivation of major histocompatibility complex class II genes. Mol Cell Biol. 2000;20:8489-98 pubmed
    ..These results support a novel function for acetylation, i.e., to regulate gene expression by stimulating the nuclear accumulation of an activator. ..
  7. Kardassis D, Pardali K, Zannis V. SMAD proteins transactivate the human ApoCIII promoter by interacting physically and functionally with hepatocyte nuclear factor 4. J Biol Chem. 2000;275:41405-14 pubmed
  8. Pinakoulaki E, Pfitzner U, Ludwig B, Varotsis C. The role of the cross-link His-Tyr in the functional properties of the binuclear center in cytochrome c oxidase. J Biol Chem. 2002;277:13563-8 pubmed
    ..Oxygenated intermediates are formed by mixing oxygen with the CO-bound mixed-valence wild type and Y280H enzymes with similar Soret maxima at 438 nm. ..
  9. Fragiadakis G, Tzamarias D, Alexandraki D. Nhp6 facilitates Aft1 binding and Ssn6 recruitment, both essential for FRE2 transcriptional activation. EMBO J. 2004;23:333-42 pubmed
    ..Therefore, transcriptional activation in response to iron availability involves multiple protein interactions between the Aft1 iron-responsive DNA-binding factor and global regulators such as Nhp6 and Ssn6. ..
  10. Fadouloglou V, Deli A, Glykos N, Psylinakis E, Bouriotis V, Kokkinidis M. Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus. FEBS J. 2007;274:3044-54 pubmed publisher
    ..The functional implications and a possible catalytic mechanism are discussed...
  11. Alcock F, Grossmann J, Gentle I, Likic V, Lithgow T, Tokatlidis K. Conserved substrate binding by chaperones in the bacterial periplasm and the mitochondrial intermembrane space. Biochem J. 2008;409:377-87 pubmed
  12. Kasotakis E, Mossou E, Adler Abramovich L, Mitchell E, Forsyth V, Gazit E, et al. Design of metal-binding sites onto self-assembled peptide fibrils. Biopolymers. 2009;92:164-72 pubmed publisher
    ..Short, biologically inspired self-assembling peptide scaffolds derived from natural fibrous proteins with known three-dimensional structure may provide a viable approach towards the rational design of inorganic nanowires. ..
  13. Sideris D, Petrakis N, Katrakili N, Mikropoulou D, Gallo A, Ciofi Baffoni S, et al. A novel intermembrane space-targeting signal docks cysteines onto Mia40 during mitochondrial oxidative folding. J Cell Biol. 2009;187:1007-22 pubmed publisher