Experts and Doctors on sequence alignment in Dortmund, North Rhine Westphalia, Germany


Locale: Dortmund, North Rhine Westphalia, Germany
Topic: sequence alignment

Top Publications

  1. Scheffzek K, Klebe C, Fritz Wolf K, Kabsch W, Wittinghofer A. Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form. Nature. 1995;374:378-81 pubmed
    ..The amino-terminal (amino-acid residues MAAQGEP) stretch and the acidic tail (DEDDDL) appear to be flexible in the crystal structure. ..
  2. Vetter I, Arndt A, Kutay U, Gorlich D, Wittinghofer A. Structural view of the Ran-Importin beta interaction at 2.3 A resolution. Cell. 1999;97:635-46 pubmed
    ..They form an irregular crescent, the concave site of which forms the interface with Ran-triphosphate. The importin-binding site of Ran does not overlap with that of the Ran-binding domain of RanBP2. ..
  3. Scrima A, Vetter I, Armengod M, Wittinghofer A. The structure of the TrmE GTP-binding protein and its implications for tRNA modification. EMBO J. 2005;24:23-33 pubmed publisher
    ..We propose a reaction mechanism whereby TrmE actively participates in the formylation reaction of uridine and regulates the ensuing hydrogenation reaction of a Schiff's base intermediate...
  4. Harjes S, Bayer P, Scheidig A. The crystal structure of human PAPS synthetase 1 reveals asymmetry in substrate binding. J Mol Biol. 2005;347:623-35 pubmed
    ..These observations strongly indicate structural changes during the reaction cycle. Furthermore crystals soaked with ADP and APS could be prepared and the corresponding structures could be solved. ..
  5. Lavie A, Ostermann N, Brundiers R, Goody R, Reinstein J, Konrad M, et al. Structural basis for efficient phosphorylation of 3'-azidothymidine monophosphate by Escherichia coli thymidylate kinase. Proc Natl Acad Sci U S A. 1998;95:14045-50 pubmed
    ..coli seems to use Arg-153 from a region termed Lid instead. Thus, the binding of AZT-MP to the yeast TmpK results in the shift of a catalytic residue, which is not the case for the bacterial kinase...
  6. Liu Y, Petrovic A, Rombaut P, Mosalaganti S, Keller J, Raunser S, et al. Insights from the reconstitution of the divergent outer kinetochore of Drosophila melanogaster. Open Biol. 2016;6:150236 pubmed publisher
    ..Our studies shed light on the structural and functional organization of a highly divergent kinetochore particle. ..
  7. Kucera N, Schmalen I, Hennig S, Ollinger R, Strauss H, Grudziecki A, et al. Unwinding the differences of the mammalian PERIOD clock proteins from crystal structure to cellular function. Proc Natl Acad Sci U S A. 2012;109:3311-6 pubmed publisher
    ..Our study reveals structural and quantitative differences between the homodimeric interactions of the three mouse PERIOD homologues, which are likely to contribute to their distinct clock functions. ..
  8. Bigalke J, Czudnochowski N, Vollmuth F, Vogel Bachmayr K, Anand K, Geyer M. Formation of Tat-TAR containing ribonucleoprotein complexes for biochemical and structural analyses. Methods. 2011;53:78-84 pubmed publisher
    ..Here, we describe purification methods of the different factors employed and chromatography techniques that yield highly specific complex assemblies suitable for crystallization. ..
  9. Zahedi R, V lzing C, Schmitt A, Frien M, Jung M, Dudek J, et al. Analysis of the membrane proteome of canine pancreatic rough microsomes identifies a novel Hsp40, termed ERj7. Proteomics. 2009;9:3463-73 pubmed publisher
    ..Our results indicate that the Hsp70-chaperone network that is present in the pancreatic ER is even more complex than previously thought, and suggest that the pancreatic rER has a significant capacity for protein degradation...

More Information


  1. Hennig S, Strauss H, Vanselow K, Yildiz O, Schulze S, Arens J, et al. Structural and functional analyses of PAS domain interactions of the clock proteins Drosophila PERIOD and mouse PERIOD2. PLoS Biol. 2009;7:e94 pubmed publisher
    ..In addition, we identify the PAS-B beta-sheet surface as a versatile interaction site mediating mPER2 homodimerization in the mammalian system and dPER-dTIM heterodimer formation in the Drosophila system. ..
  2. Blankenfeldt W, Thoma N, Wray J, Gautel M, Schlichting I. Crystal structures of human cardiac beta-myosin II S2-Delta provide insight into the functional role of the S2 subfragment. Proc Natl Acad Sci U S A. 2006;103:17713-7 pubmed
    ..The observation that many disease-associated mutations affect the second negatively charged ring further suggests that charge interactions play an important role in regulation of cardiac muscle activity through myosin-binding protein C. ..
  3. Hemsath L, Dvorsky R, Fiegen D, Carlier M, Ahmadian M. An electrostatic steering mechanism of Cdc42 recognition by Wiskott-Aldrich syndrome proteins. Mol Cell. 2005;20:313-24 pubmed
    ..This process is a prerequisite for WASp activation and a critical step in temporal regulation and integration of WASp-mediated cellular responses. ..
  4. Dursina B, Thoma N, Sidorovitch V, Niculae A, Iakovenko A, Rak A, et al. Interaction of yeast Rab geranylgeranyl transferase with its protein and lipid substrates. Biochemistry. 2002;41:6805-16 pubmed
    ..Despite the ability to function in concert with yRabGGTase in vitro, expression of mammalian REP-1 could not complement deletion of MRS6 gene in S. cerevisiae in vivo. The implications of these findings are discussed. ..
  5. Kühnel K, Veltel S, Schlichting I, Wittinghofer A. Crystal structure of the human retinitis pigmentosa 2 protein and its interaction with Arl3. Structure. 2006;14:367-78 pubmed
    ..The abilitities of RP2 to bind Arl3 and cause retinitis pigmentosa seem to be correlated, since both the R118H and E138G mutants show a drastically reduced affinity to Arl3. ..
  6. Ismail S, Vetter I, Sot B, Wittinghofer A. The structure of an Arf-ArfGAP complex reveals a Ca2+ regulatory mechanism. Cell. 2010;141:812-21 pubmed publisher
    ..Calcium stimulates the GAP activity of ASAPs, but not other members of the ArfGAP family. This type of regulation is unique for GAPs and any other calcium-regulated processes and hints at a crosstalk between Ca(2+) and Arf signaling. ..
  7. Schwender H, Ickstadt K. Empirical Bayes analysis of single nucleotide polymorphisms. BMC Bioinformatics. 2008;9:144 pubmed publisher
    ..Moreover, the posterior probabilities resulting from the empirical Bayes analysis of (prespecified) interactions/genotypes can also be used to quantify the importance of these interactions. ..
  8. Würtele M, Wolf E, Pederson K, Buchwald G, Ahmadian M, Barbieri J, et al. How the Pseudomonas aeruginosa ExoS toxin downregulates Rac. Nat Struct Biol. 2001;8:23-6 pubmed
    ..Considering the intrinsic resistance of P. aeruginosa to antibiotics, this might open up a new avenue towards blocking its pathogenicity. ..
  9. Friese A, Faesen A, Huis In t Veld P, Fischböck J, Prumbaum D, Petrovic A, et al. Molecular requirements for the inter-subunit interaction and kinetochore recruitment of SKAP and Astrin. Nat Commun. 2016;7:11407 pubmed publisher
    ..Collectively, our studies represent the first thorough mechanistic analysis of SKAP and Astrin, and significantly advance our functional understanding of these important mitotic proteins. ..