Experts and Doctors on saccharomyces cerevisiae in Dortmund, North Rhine Westphalia, Germany


Locale: Dortmund, North Rhine Westphalia, Germany
Topic: saccharomyces cerevisiae

Top Publications

  1. Lavie A, Ostermann N, Brundiers R, Goody R, Reinstein J, Konrad M, et al. Structural basis for efficient phosphorylation of 3'-azidothymidine monophosphate by Escherichia coli thymidylate kinase. Proc Natl Acad Sci U S A. 1998;95:14045-50 pubmed
    ..coli seems to use Arg-153 from a region termed Lid instead. Thus, the binding of AZT-MP to the yeast TmpK results in the shift of a catalytic residue, which is not the case for the bacterial kinase...
  2. Vetter I, Arndt A, Kutay U, Gorlich D, Wittinghofer A. Structural view of the Ran-Importin beta interaction at 2.3 A resolution. Cell. 1999;97:635-46 pubmed
    ..They form an irregular crescent, the concave site of which forms the interface with Ran-triphosphate. The importin-binding site of Ran does not overlap with that of the Ran-binding domain of RanBP2. ..
  3. Seewald M, Kraemer A, Farkasovsky M, Körner C, Wittinghofer A, Vetter I. Biochemical characterization of the Ran-RanBP1-RanGAP system: are RanBP proteins and the acidic tail of RanGAP required for the Ran-RanGAP GTPase reaction?. Mol Cell Biol. 2003;23:8124-36 pubmed
    ..Rather, genetic experiments in Saccharomyces cerevisiae demonstrate a profound effect of the acidic tail on microtubule organization during mitosis. We propose that the acidic tail of RanGAP is required for a process during mitosis. ..
  4. Schrader N, Stelter P, Flemming D, Kunze R, Hurt E, Vetter I. Structural basis of the nic96 subcomplex organization in the nuclear pore channel. Mol Cell. 2008;29:46-55 pubmed publisher
    ..Notably, Nic96DeltaN binds in vitro to FG repeats of the Nsp1 complex. These data suggest a model of how Nic96 could organize a transport module with coiled-coil domains and FG repeats in the central pore channel. ..
  5. Sirajuddin M, Farkasovsky M, Zent E, Wittinghofer A. GTP-induced conformational changes in septins and implications for function. Proc Natl Acad Sci U S A. 2009;106:16592-7 pubmed publisher
    ..These data also offer a first rationale for subdividing human septins into different functional subgroups. ..
  6. Grzegorczyk M, Husmeier D. Improvements in the reconstruction of time-varying gene regulatory networks: dynamic programming and regularization by information sharing among genes. Bioinformatics. 2011;27:693-9 pubmed publisher
    ..A MATLAB implementation of the algorithm and a supplementary paper with algorithmic details and further results for the Arabidopsis data can be downloaded from: ..
  7. Blank L, Desphande R, Schmid A, Hayen H. Analysis of carbon and nitrogen co-metabolism in yeast by ultrahigh-resolution mass spectrometry applying 13C- and 15N-labeled substrates simultaneously. Anal Bioanal Chem. 2012;403:2291-305 pubmed publisher
    ..The results indicate that not only glutamate but also alanine acts as an amino donor during alanine and valine synthesis. Metabolic studies using FTICR-MS can exploit new possibilities by the use of multiple-labeled elemental isotopes...
  8. Becker J, Melchior F, Gerke V, Bischoff F, Ponstingl H, Wittinghofer A. RNA1 encodes a GTPase-activating protein specific for Gsp1p, the Ran/TC4 homologue of Saccharomyces cerevisiae. J Biol Chem. 1995;270:11860-5 pubmed
    ..The GTP hydrolysis of Ran is induced by RanGAP1 and rna1p but not by Rna1p. Implications for the suggested functions of Ran/TC4/Gsp1p in nuclear transport and mitotic control are discussed. ..
  9. Dursina B, Thoma N, Sidorovitch V, Niculae A, Iakovenko A, Rak A, et al. Interaction of yeast Rab geranylgeranyl transferase with its protein and lipid substrates. Biochemistry. 2002;41:6805-16 pubmed
    ..Despite the ability to function in concert with yRabGGTase in vitro, expression of mammalian REP-1 could not complement deletion of MRS6 gene in S. cerevisiae in vivo. The implications of these findings are discussed. ..

More Information


  1. Pylypenko O, Schönichen A, Ludwig D, Ungermann C, Goody R, Rak A, et al. Farnesylation of the SNARE protein Ykt6 increases its stability and helical folding. J Mol Biol. 2008;377:1334-45 pubmed publisher
    ..Together, these observations support a previously suggested closed conformation of cytosolic Ykt6, where the C-terminal farnesyl moiety folds onto a hydrophobic groove in the N-terminal longin domain. ..
  2. Ignatev A, Kravchenko S, Rak A, Goody R, Pylypenko O. A structural model of the GDP dissociation inhibitor rab membrane extraction mechanism. J Biol Chem. 2008;283:18377-84 pubmed publisher
    ..We suggest a model for the interaction of GDI with prenylated Rab proteins that incorporates a stepwise increase in affinity as the three different partial interactions are successively formed. ..
  3. Sonntag T, Mootz H. An intein-cassette integration approach used for the generation of a split TEV protease activated by conditional protein splicing. Mol Biosyst. 2011;7:2031-9 pubmed publisher
  4. Venne A, Vögtle F, Meisinger C, Sickmann A, Zahedi R. Novel highly sensitive, specific, and straightforward strategy for comprehensive N-terminal proteomics reveals unknown substrates of the mitochondrial peptidase Icp55. J Proteome Res. 2013;12:3823-30 pubmed publisher
    ..Thus, ChaFRADIC is a powerful and practicable tool for protease and peptidase research, providing the sensitivity to characterize even samples that can be obtained only in small quantities. ..
  5. Czarnotta E, Dianat M, Korf M, Granica F, Merz J, Maury J, et al. Fermentation and purification strategies for the production of betulinic acid and its lupane-type precursors in Saccharomyces cerevisiae. Biotechnol Bioeng. 2017;114:2528-2538 pubmed publisher
    ..This study highlights the potential of microbial production of plant derived triterpenoids in S. cerevisiae by combining metabolic and process engineering. ..
  6. Itzen A, Rak A, Goody R. Sec2 is a highly efficient exchange factor for the Rab protein Sec4. J Mol Biol. 2007;365:1359-67 pubmed