Experts and Doctors on saccharomyces cerevisiae in Bochum, North Rhine Westphalia, Germany


Locale: Bochum, North Rhine Westphalia, Germany
Topic: saccharomyces cerevisiae

Top Publications

  1. Debelyy M, Platta H, Saffian D, Hensel A, Thoms S, Meyer H, et al. Ubp15p, a ubiquitin hydrolase associated with the peroxisomal export machinery. J Biol Chem. 2011;286:28223-34 pubmed publisher
    ..The results merge into a picture in which removal of ubiquitin from the PTS1 receptor Pex5p is a specific event and might represent a vital step in receptor recycling...
  2. Grunau S, Lay D, Mindthoff S, Platta H, Girzalsky W, Just W, et al. The phosphoinositide 3-kinase Vps34p is required for pexophagy in Saccharomyces cerevisiae. Biochem J. 2011;434:161-70 pubmed publisher
    ..This demonstrates that PtdIns3P-mediated signalling is required for pexophagy. ..
  3. Agne B, Meindl N, Niederhoff K, Einwächter H, Rehling P, Sickmann A, et al. Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery. Mol Cell. 2003;11:635-46 pubmed
    ..We conclude that Pex8p organizes the formation of the larger import complex from the trans side of the peroxisomal membrane and thus might enable functional communication between both sides of the membrane. ..
  4. Eckert J, Johnsson N. Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome. J Cell Sci. 2003;116:3623-34 pubmed
    ..A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions. ..
  5. Rottensteiner H, Kramer A, Lorenzen S, Stein K, Landgraf C, Volkmer Engert R, et al. Peroxisomal membrane proteins contain common Pex19p-binding sites that are an integral part of their targeting signals. Mol Biol Cell. 2004;15:3406-17 pubmed
    ..By adding the heterologous binding site of Pex11p, peroxisomal targeting of the Pex13p fragment was restored. We conclude that Pex19p-binding sites are well-defined entities that represent an essential part of the mPTS. ..
  6. Halbach A, Lorenzen S, Landgraf C, Volkmer Engert R, Erdmann R, Rottensteiner H. Function of the PEX19-binding site of human adrenoleukodystrophy protein as targeting motif in man and yeast. PMP targeting is evolutionarily conserved. J Biol Chem. 2005;280:21176-82 pubmed
    ..Our data clearly revealed a role for PEX19-binding sites as PMP-targeting motifs across species, thereby demonstrating the evolutionary conservation of PMP signal sequences from yeast to man...
  7. Mayrhofer S, Pöggeler S. Functional characterization of an alpha-factor-like Sordaria macrospora peptide pheromone and analysis of its interaction with its cognate receptor in Saccharomyces cerevisiae. Eukaryot Cell. 2005;4:661-72 pubmed
    ..However, loss of the ppg1 gene does not affect vegetative growth or fertility. Finally, we established the yeast assay as an easy and useful system for analyzing pheromone production in developmental mutants of S. macrospora. ..
  8. Erdmann R, Veenhuis M, Mertens D, Kunau W. Isolation of peroxisome-deficient mutants of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1989;86:5419-23 pubmed
    ..These pas mutants may provide useful tools for future studies on the molecular mechanisms involved in peroxisomal assembly. ..
  9. Halbach A, Landgraf C, Lorenzen S, Rosenkranz K, Volkmer Engert R, Erdmann R, et al. Targeting of the tail-anchored peroxisomal membrane proteins PEX26 and PEX15 occurs through C-terminal PEX19-binding sites. J Cell Sci. 2006;119:2508-17 pubmed
    ..We conclude that C-terminal PEX19-binding sites mark tail-anchored proteins for delivery to peroxisomes. ..

More Information


  1. Rucktäschel R, Halbach A, Girzalsky W, Rottensteiner H, Erdmann R. De novo synthesis of peroxisomes upon mitochondrial targeting of Pex3p. Eur J Cell Biol. 2010;89:947-54 pubmed publisher
    ..We conclude that natural or artificial targeting of Pex3p to any endomembrane may initiate peroxisome formation and that also Pex3p-containing mitochondria can serve as source for the de novo synthesis of peroxisomes. ..
  2. Rucktäschel R, Thoms S, Sidorovitch V, Halbach A, Pechlivanis M, Volkmer R, et al. Farnesylation of pex19p is required for its structural integrity and function in peroxisome biogenesis. J Biol Chem. 2009;284:20885-96 pubmed publisher
    ..Thus, isoprenylation of Pex19p contributes to substrate membrane protein recognition for the topogenesis of PMPs, and our results highlight the importance of lipid modifications in protein-protein interactions. ..
  3. Niederhoff K, Meindl Beinker N, Kerssen D, Perband U, Schafer A, Schliebs W, et al. Yeast Pex14p possesses two functionally distinct Pex5p and one Pex7p binding sites. J Biol Chem. 2005;280:35571-8 pubmed
  4. Kohl M, Wiese S, Warscheid B. Cytoscape: software for visualization and analysis of biological networks. Methods Mol Biol. 2011;696:291-303 pubmed publisher
    ..Furthermore, special features of alternative software tools are highlighted in order to assist researchers in the choice of an adequate program for their specific requirements. ..
  5. Grunau S, Schliebs W, Linnepe R, Neufeld C, Cizmowski C, Reinartz B, et al. Peroxisomal targeting of PTS2 pre-import complexes in the yeast Saccharomyces cerevisiae. Traffic. 2009;10:451-60 pubmed publisher
  6. Halbach A, Rucktäschel R, Rottensteiner H, Erdmann R. The N-domain of Pex22p can functionally replace the Pex3p N-domain in targeting and peroxisome formation. J Biol Chem. 2009;284:3906-16 pubmed publisher
    ..The C-terminal domain of Pex3p thus contains the relevant information required for de novo peroxisome formation, thereby challenging the concept of the N terminus of Pex3p being key in that process. ..
  7. Papatheodorou P, Domanska G, Rassow J. Protein targeting to mitochondria of Saccharomyces cerevisiae and Neurospora crassa: in vitro and in vivo studies. Methods Mol Biol. 2007;390:151-66 pubmed
    ..Assays are available for studies on the import pathways and localization of mitochondrial proteins and for the characterization of the components of the protein import machinery. ..
  8. Funke S, Knechten T, Ollesch J, Schünemann D. A unique sequence motif in the 54-kDa subunit of the chloroplast signal recognition particle mediates binding to the 43-kDa subunit. J Biol Chem. 2005;280:8912-7 pubmed
    ..Alignment studies revealed that the cpSRP43-binding site is conserved in chloroplast SRP54 proteins and is not present in any SRP54 subunit of cytosolic SRPs. ..
  9. Will G, Soukupova M, Hong X, Erdmann K, Kiel J, Dodt G, et al. Identification and characterization of the human orthologue of yeast Pex14p. Mol Cell Biol. 1999;19:2265-77 pubmed
    ..The role of HsPex14p in mammalian peroxisome biogenesis makes HsPEX14 a candidate PBD gene for being responsible for an unrecognized complementation group of human peroxisome biogenesis disorders. ..
  10. Henke B, Girzalsky W, Berteaux Lecellier V, Erdmann R. IDP3 encodes a peroxisomal NADP-dependent isocitrate dehydrogenase required for the beta-oxidation of unsaturated fatty acids. J Biol Chem. 1998;273:3702-11 pubmed
    ..cerevisiae contain the enzyme equipment needed for the degradation of unsaturated fatty acids, including an NADP-dependent isocitrate dehydrogenase, a putative constituent of a peroxisomal NADPH-regenerating redox system. ..