Experts and Doctors on molecular sequence data in Wuppertal, North Rhine Westphalia, Germany
Locale: Wuppertal, North Rhine Westphalia, Germany
Topic: molecular sequence data
Ehlert K, Schr der W, Labischinski H. Specificities of FemA and FemB for different glycine residues: FemB cannot substitute for FemA in staphylococcal peptidoglycan pentaglycine side chain formation. J Bacteriol. 1997;179:7573-6 pubmed
..Although FemB has 39% identity with FemA, it cannot substitute for FemA. The FemA and FemB proteins seem to be highly specific in regard to the position of the glycine residues that they attach...
Rumsfeld J, Ziegelbauer K, Spaltmann F. High-throughput assay for inorganic pyrophosphatases using the cytosolic enzymes of Saccharomyces cerevisiae and human as an example. Protein Expr Purif. 2000;18:303-9 pubmed
..The resulting complex was quantified spectrophotometrically. The assay was adapted to a microtiter plate format. Thus, it is possible to screen a large compound pool for iPPase inhibitors in a short period of time. ..
Freiberg C, Wieland B, Spaltmann F, Ehlert K, Brötz H, Labischinski H. Identification of novel essential Escherichia coli genes conserved among pathogenic bacteria. J Mol Microbiol Biotechnol. 2001;3:483-9 pubmed
..These findings demonstrate the potential of such proteins to be used in screening of large chemical libraries for inhibitors which could be further developed to novel broad-spectrum antibiotics. ..
Kaschabek S, Kuhn B, Müller D, Schmidt E, Reineke W. Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: purification and characterization of 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase. J Bacteriol. 2002;184:207-15 pubmed
Göbel M, Kassel Cati K, Schmidt E, Reineke W. Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: cloning, characterization, and analysis of sequences encoding 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase. J Bacteriol. 2002;184:216-23 pubmed
..An incomplete ORF6 of 1,183 bp downstream of ORF5 and oriented in the opposite direction was found. The protein sequence deduced from ORF6 showed a putative AMP-binding domain signature. ..
Hortner M, Nielsch U, Mayr L, Heinrich P, Haan S. A new high affinity binding site for suppressor of cytokine signaling-3 on the erythropoietin receptor. Eur J Biochem. 2002;269:2516-26 pubmed
Oehme F, Ellinghaus P, Kolkhof P, Smith T, Ramakrishnan S, Hütter J, et al
. Overexpression of PH-4, a novel putative proline 4-hydroxylase, modulates activity of hypoxia-inducible transcription factors. Biochem Biophys Res Commun. 2002;296:343-9 pubmed
..Suppression of transactivation was correlated with decrease of cellular contents of HIF. Thus, PH-4 might be related to cellular oxygen sensing. ..
Möhlmann S, Bringmann P, Greven S, Harrenga A. Site-specific modification of ED-B-targeting antibody using intein-fusion technology. BMC Biotechnol. 2011;11:76 pubmed publisher
..The described protocols can be used to prepare immunoconjugates of high homogeneity and with a defined drug load of two. The attachment to the C-termini is expected to retain the affinity and effector functions of the antibodies. ..
Hoffmann L, Schmidt P, Keim Y, Hoffmann C, Schmidt H, Stasch J. Fluorescence dequenching makes haem-free soluble guanylate cyclase detectable in living cells. PLoS ONE. 2011;6:e23596 pubmed publisher
..Nevertheless, it allows to monitor sGC's redox regulation in living cells and future enhancements might be able to extend this approach to in vivo conditions. ..