Experts and Doctors on kinetics in Bochum, North Rhine Westphalia, Germany


Locale: Bochum, North Rhine Westphalia, Germany
Topic: kinetics

Top Publications

  1. Mullershausen F, Russwurm M, Koesling D, Friebe A. In vivo reconstitution of the negative feedback in nitric oxide/cGMP signaling: role of phosphodiesterase type 5 phosphorylation. Mol Biol Cell. 2004;15:4023-30 pubmed
    ..Phosphorylation of PDE5 seems to act as memory switch for activation leading to long-term desensitization of the signaling pathway. ..
  2. Lehmann T, Pollmann S. Gene expression and characterization of a stress-induced tyrosine decarboxylase from Arabidopsis thaliana. FEBS Lett. 2009;583:1895-900 pubmed publisher
    ..5-9. Recombinant TyrDC protein formed tetramers, as shown by blue native gel electrophoresis. ..
  3. Vöpel T, Kunzelmann S, Herrmann C. Nucleotide dependent cysteine reactivity of hGBP1 uncovers a domain movement during GTP hydrolysis. FEBS Lett. 2009;583:1923-7 pubmed publisher
    ..Our results indicate that nucleotide hydrolysis induces a domain movement in hGBP1, which we suggest enables further assembly of the protein. ..
  4. Doerner J, Hatt H, Ramsey I. Voltage- and temperature-dependent activation of TRPV3 channels is potentiated by receptor-mediated PI(4,5)P2 hydrolysis. J Gen Physiol. 2011;137:271-88 pubmed publisher
    ..PI(4,5)P(2)-dependent modulation of TRPV3 activity represents an attractive mechanism for acute regulation of keratinocyte signaling cascades that control cell proliferation and the release of autocrine and paracrine factors. ..
  5. Syguda A, Bauer M, Benscheid U, Ostler N, Naschberger E, Ince S, et al. Tetramerization of human guanylate-binding protein 1 is mediated by coiled-coil formation of the C-terminal ?-helices. FEBS J. 2012;279:2544-54 pubmed publisher
    ..Furthermore, we can demonstrate the biological relevance of hGBP1 tetramer formation in living cells by chemical cross-link experiments. ..
  6. Bartz H, Büning Pfaue F, Türkel O, Schauer U. Respiratory syncytial virus induces prostaglandin E2, IL-10 and IL-11 generation in antigen presenting cells. Clin Exp Immunol. 2002;129:438-45 pubmed
    ..Since IL-10, IL-11 and PGE2 are known to act immunosuppressive rather than proinflammatory, these mediators might be responsible for the delayed protective RSV specific immune response. ..
  7. Meier J, Nauck M, Kranz D, Holst J, Deacon C, Gaeckler D, et al. Secretion, degradation, and elimination of glucagon-like peptide 1 and gastric inhibitory polypeptide in patients with chronic renal insufficiency and healthy control subjects. Diabetes. 2004;53:654-62 pubmed
    ..Delayed elimination of GLP-1 and GIP in renal insufficiency may influence the pharmacokinetics and pharmacodynamics of dipeptidyl peptidase IV-resistant incretin derivatives to be used for the treatment of patients with type 2 diabetes. ..
  8. Ballweber E, Hannappel E, Huff T, Stephan H, Haener M, Taschner N, et al. Polymerisation of chemically cross-linked actin:thymosin beta(4) complex to filamentous actin: alteration in helical parameters and visualisation of thymosin beta(4) binding on F-actin. J Mol Biol. 2002;315:613-25 pubmed
    ..However, parts of the difference map might represent subtle conformational changes of both proteins occurring upon complex formation. ..
  9. Richardt A, Kemme T, Wagner S, Schwarzer D, Marahiel M, Hovemann B. Ebony, a novel nonribosomal peptide synthetase for beta-alanine conjugation with biogenic amines in Drosophila. J Biol Chem. 2003;278:41160-6 pubmed
    ..We propose that in the Drosophila eye Ebony is involved in the transmission process by inactivation of histamine through beta-alanyl conjugation. ..

More Information


  1. Halbach A, Lorenzen S, Landgraf C, Volkmer Engert R, Erdmann R, Rottensteiner H. Function of the PEX19-binding site of human adrenoleukodystrophy protein as targeting motif in man and yeast. PMP targeting is evolutionarily conserved. J Biol Chem. 2005;280:21176-82 pubmed
    ..Our data clearly revealed a role for PEX19-binding sites as PMP-targeting motifs across species, thereby demonstrating the evolutionary conservation of PMP signal sequences from yeast to man...
  2. Winkler M, K ck U. The group IIB intron from the green alga Scenedesmus obliquus mitochondrion: molecular characterization of the in vitro splicing products. Curr Genet. 1991;20:495-502 pubmed
    ..The predicted secondary and tertiary structure of intron rI1 of S. obliquus contains all the motifs necessary for optimal self-splicing and which are characteristic of other group IIB introns from different species...
  3. Russwurm M, Mullershausen F, Friebe A, Jäger R, Russwurm C, Koesling D. Design of fluorescence resonance energy transfer (FRET)-based cGMP indicators: a systematic approach. Biochem J. 2007;407:69-77 pubmed
    ..The in vivo performance of these new indicators is demonstrated in living cells and validated by comparison with cGMP dynamics as measured by radioimmunoassays. ..
  4. Gisk B, Yasui Y, Kohchi T, Frankenberg Dinkel N. Characterization of the haem oxygenase protein family in Arabidopsis thaliana reveals a diversity of functions. Biochem J. 2010;425:425-34 pubmed publisher
    ..However, HO2 shows strong binding of proto IX (protoporphyrin IX), a precursor for both haem and chlorophyll biosynthesis. A possible function of HO2 in the regulation of tetrapyrrole metabolism is discussed. ..
  5. Reuber B, Karl C, Reimann S, Mihalik S, Dodt G. Cloning and functional expression of a mammalian gene for a peroxisomal sarcosine oxidase. J Biol Chem. 1997;272:6766-76 pubmed
  6. Sch kermann M, Langklotz S, Narberhaus F. FtsH-mediated coordination of lipopolysaccharide biosynthesis in Escherichia coli correlates with the growth rate and the alarmone (p)ppGpp. J Bacteriol. 2013;195:1912-9 pubmed publisher
    ..Our data provide new insights into the essential control of LPS biosynthesis in E. coli...
  7. Kunzelmann S, Praefcke G, Herrmann C. Transient kinetic investigation of GTP hydrolysis catalyzed by interferon-gamma-induced hGBP1 (human guanylate binding protein 1). J Biol Chem. 2006;281:28627-35 pubmed
    ..The assembled forms of the GDP- and GMP-bound states of hGBP1 are accessible only through GTP binding and hydrolysis and achieve a lifetime of a few seconds. ..
  8. Gavriljuk K, Gazdag E, Itzen A, Kötting C, Goody R, Gerwert K. Catalytic mechanism of a mammalian Rab·RabGAP complex in atomic detail. Proc Natl Acad Sci U S A. 2012;109:21348-53 pubmed publisher
    ..The combination of X-ray structure analysis and time-resolved FTIR spectroscopy provides detailed insight in the catalysis of human Rab GTPases. ..
  9. Esimone C, Eck G, Duong T, Uberla K, Proksch P, Grunwald T. Potential anti-respiratory syncytial virus lead compounds from Aglaia species. Pharmazie. 2008;63:768-73 pubmed
    ..Therefore, dammarenolic acid, aglaiol and niloticin demonstrate potent anti-RSV activity that shouldbe explored further in the current search for anti-RSV therapeutic agents...
  10. Jenrich R, Trompetter I, Bak S, Olsen C, Møller B, Piotrowski M. Evolution of heteromeric nitrilase complexes in Poaceae with new functions in nitrile metabolism. Proc Natl Acad Sci U S A. 2007;104:18848-53 pubmed
    ..Through combinatorial biochemistry and neofunctionalizations, the small family of nitrilases has gained diverse biological functions in nitrile metabolism...
  11. Mannherz H, Ballweber E, Galla M, Villard S, Granier C, Steegborn C, et al. Mapping the ADF/cofilin binding site on monomeric actin by competitive cross-linking and peptide array: evidence for a second binding site on monomeric actin. J Mol Biol. 2007;366:745-55 pubmed
    ..In the absence of any crystal structures of ADF or cofilin in complex with actin, these studies provide further information about the binding sites on F-actin for these important actin regulatory proteins. ..
  12. Schinkel C, Wick M, Muhr G, Koller M. Analysis of systemic interleukin-11 after major trauma. Shock. 2005;23:30-4 pubmed
    ..In contrast to IL-6, IL-11 in plasma does not correlate with trauma severity and seems to have no clinical relevance to outcome prediction after trauma. ..
  13. Ohm T, Wegner A. Random copolymerization of ATP-actin and ADP-actin. Biochemistry. 1991;30:11193-7 pubmed
    ..According to the analysis of the critical concentrations, the equilibrium constants for incorporation of ATP-actin or ADP-actin into filaments were independent of the type of nucleotide bound to contiguous subunits. ..
  14. Lillig C, Prior A, Schwenn J, Aslund F, Ritz D, Vlamis Gardikas A, et al. New thioredoxins and glutaredoxins as electron donors of 3'-phosphoadenylylsulfate reductase. J Biol Chem. 1999;274:7695-8 pubmed
    ..9 microM), whereas the Km trx2 was considerably higher (34.2 microM). The results agree with previous in vivo data suggesting that Trx1 or Grx1 is essential for sulfate reduction but not for ribonucleotide reduction in E. coli. ..